UniProtKB - P05852 (TSAD_ECOLI)
tRNA N6-adenosine threonylcarbamoyltransferase
tsaD
Functioni
Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction. May also be involved in the metabolism of glycated proteins, but does not show sialoglycoprotease activity against glycophorin A.
3 PublicationsMiscellaneous
Caution
Catalytic activityi
- adenosine37 in tRNA + L-threonylcarbamoyladenylate = AMP + H+ + N6-L-threonylcarbamoyladenosine37 in tRNAUniRule annotation1 PublicationEC:2.3.1.234UniRule annotation1 Publication
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 111 | IronUniRule annotation | 1 | |
Metal bindingi | 115 | IronUniRule annotation | 1 | |
Binding sitei | 167 | SubstrateUniRule annotation | 1 | |
Binding sitei | 180 | Substrate; via amide nitrogenUniRule annotation | 1 | |
Binding sitei | 272 | SubstrateUniRule annotation | 1 | |
Metal bindingi | 300 | IronUniRule annotation | 1 |
GO - Molecular functioni
- glycosylation-dependent protein binding Source: EcoCyc
- identical protein binding Source: EcoCyc
- iron ion binding Source: EcoCyc
- magnesium ion binding Source: EcoCyc
- N(6)-L-threonylcarbamoyladenine synthase activity Source: UniProtKB-EC
GO - Biological processi
- tRNA threonylcarbamoyladenosine modification Source: ComplexPortal
Keywordsi
Molecular function | Acyltransferase, Transferase |
Biological process | tRNA processing |
Ligand | Iron, Metal-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11171-MONOMER |
BRENDAi | 2.3.1.234, 2026 |
Names & Taxonomyi
Protein namesi | Recommended name: tRNA N6-adenosine threonylcarbamoyltransferaseUniRule annotation (EC:2.3.1.234UniRule annotation1 Publication)Alternative name(s): N6-L-threonylcarbamoyladenine synthaseUniRule annotation Short name: t(6)A synthaseUniRule annotation t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaDUniRule annotation tRNA threonylcarbamoyladenosine biosynthesis protein TsaDUniRule annotation |
Gene namesi | Name:tsaDUniRule annotation Synonyms:gcp, ygjD Ordered Locus Names:b3064, JW3036 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation1 Publication
Cytosol
- cytosol Source: EcoCyc
Other locations
- EKC/KEOPS complex Source: ComplexPortal
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000096962 | 1 – 337 | tRNA N6-adenosine threonylcarbamoyltransferaseAdd BLAST | 337 |
Post-translational modificationi
Proteomic databases
jPOSTi | P05852 |
PaxDbi | P05852 |
PRIDEi | P05852 |
Interactioni
Subunit structurei
Homodimer.
Interacts with TsaB; may form a heterodimer with TsaB.
Also interacts with TsaC.
4 PublicationsBinary interactionsi
P05852
With | #Exp. | IntAct |
---|---|---|
tsaB [P76256] | 8 | EBI-561994,EBI-560669 |
GO - Molecular functioni
- glycosylation-dependent protein binding Source: EcoCyc
- identical protein binding Source: EcoCyc
Protein-protein interaction databases
BioGRIDi | 4261402, 87 interactors 851894, 3 interactors |
ComplexPortali | CPX-1094, YgjD-YeaZ-YjeE complex |
DIPi | DIP-9749N |
IntActi | P05852, 14 interactors |
STRINGi | 511145.b3064 |
Structurei
Secondary structure
3D structure databases
SMRi | P05852 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 134 – 138 | Substrate bindingUniRule annotation | 5 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0533, Bacteria |
HOGENOMi | CLU_023208_0_2_6 |
InParanoidi | P05852 |
PhylomeDBi | P05852 |
Family and domain databases
HAMAPi | MF_01445, TsaD, 1 hit |
InterProi | View protein in InterPro IPR043129, ATPase_NBD IPR000905, Gcp-like_dom IPR017861, KAE1/TsaD IPR017860, Peptidase_M22_CS IPR022450, TsaD |
PANTHERi | PTHR11735, PTHR11735, 1 hit PTHR11735:SF6, PTHR11735:SF6, 1 hit |
Pfami | View protein in Pfam PF00814, TsaD, 1 hit |
PRINTSi | PR00789, OSIALOPTASE |
SUPFAMi | SSF53067, SSF53067, 1 hit |
TIGRFAMsi | TIGR00329, gcp_kae1, 1 hit TIGR03723, T6A_TsaD_YgjD, 1 hit |
PROSITEi | View protein in PROSITE PS01016, GLYCOPROTEASE, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MRVLGIETSC DETGIAIYDD EKGLLANQLY SQVKLHADYG GVVPELASRD
60 70 80 90 100
HVRKTVPLIQ AALKESGLTA KDIDAVAYTA GPGLVGALLV GATVGRSLAF
110 120 130 140 150
AWDVPAIPVH HMEGHLLAPM LEDNPPEFPF VALLVSGGHT QLISVTGIGQ
160 170 180 190 200
YELLGESIDD AAGEAFDKTA KLLGLDYPGG PLLSKMAAQG TAGRFVFPRP
210 220 230 240 250
MTDRPGLDFS FSGLKTFAAN TIRDNGTDDQ TRADIARAFE DAVVDTLMIK
260 270 280 290 300
CKRALDQTGF KRLVMAGGVS ANRTLRAKLA EMMKKRRGEV FYARPEFCTD
310 320 330
NGAMIAYAGM VRFKAGATAD LGVSVRPRWP LAELPAA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 136 | S → C in AAA72575 (PubMed:3297921).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M16194 Genomic DNA Translation: AAA72575.1 U28379 Genomic DNA Translation: AAA89144.1 U00096 Genomic DNA Translation: AAC76100.1 AP009048 Genomic DNA Translation: BAE77115.1 |
PIRi | F65094, QQECR6 |
RefSeqi | NP_417536.1, NC_000913.3 WP_001264352.1, NZ_STEB01000001.1 |
Genome annotation databases
EnsemblBacteriai | AAC76100; AAC76100; b3064 BAE77115; BAE77115; BAE77115 |
GeneIDi | 947578 |
KEGGi | ecj:JW3036 eco:b3064 |
PATRICi | fig|1411691.4.peg.3666 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M16194 Genomic DNA Translation: AAA72575.1 U28379 Genomic DNA Translation: AAA89144.1 U00096 Genomic DNA Translation: AAC76100.1 AP009048 Genomic DNA Translation: BAE77115.1 |
PIRi | F65094, QQECR6 |
RefSeqi | NP_417536.1, NC_000913.3 WP_001264352.1, NZ_STEB01000001.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4WQ4 | X-ray | 2.33 | A/B | 1-337 | [»] | |
4WQ5 | X-ray | 2.33 | A/B | 1-337 | [»] | |
4YDU | X-ray | 2.33 | A/B | 1-337 | [»] | |
6Z81 | X-ray | 2.31 | A/B | 1-337 | [»] | |
SMRi | P05852 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261402, 87 interactors 851894, 3 interactors |
ComplexPortali | CPX-1094, YgjD-YeaZ-YjeE complex |
DIPi | DIP-9749N |
IntActi | P05852, 14 interactors |
STRINGi | 511145.b3064 |
Proteomic databases
jPOSTi | P05852 |
PaxDbi | P05852 |
PRIDEi | P05852 |
Protocols and materials databases
DNASUi | 947578 |
Genome annotation databases
EnsemblBacteriai | AAC76100; AAC76100; b3064 BAE77115; BAE77115; BAE77115 |
GeneIDi | 947578 |
KEGGi | ecj:JW3036 eco:b3064 |
PATRICi | fig|1411691.4.peg.3666 |
Organism-specific databases
EchoBASEi | EB1158 |
Phylogenomic databases
eggNOGi | COG0533, Bacteria |
HOGENOMi | CLU_023208_0_2_6 |
InParanoidi | P05852 |
PhylomeDBi | P05852 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11171-MONOMER |
BRENDAi | 2.3.1.234, 2026 |
Miscellaneous databases
PROi | PR:P05852 |
Family and domain databases
HAMAPi | MF_01445, TsaD, 1 hit |
InterProi | View protein in InterPro IPR043129, ATPase_NBD IPR000905, Gcp-like_dom IPR017861, KAE1/TsaD IPR017860, Peptidase_M22_CS IPR022450, TsaD |
PANTHERi | PTHR11735, PTHR11735, 1 hit PTHR11735:SF6, PTHR11735:SF6, 1 hit |
Pfami | View protein in Pfam PF00814, TsaD, 1 hit |
PRINTSi | PR00789, OSIALOPTASE |
SUPFAMi | SSF53067, SSF53067, 1 hit |
TIGRFAMsi | TIGR00329, gcp_kae1, 1 hit TIGR03723, T6A_TsaD_YgjD, 1 hit |
PROSITEi | View protein in PROSITE PS01016, GLYCOPROTEASE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TSAD_ECOLI | |
Accessioni | P05852Primary (citable) accession number: P05852 Secondary accession number(s): Q2M9E1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1988 |
Last sequence update: | August 29, 2003 | |
Last modified: | February 23, 2022 | |
This is version 184 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families