Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Bacteriocin microcin B17

Gene

mcbA

Organism
Escherichia coli
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This glycine-rich peptide antibiotic inhibits DNA replication in many enteric bacteria, that leads to induction of the SOS repair system, massive DNA degradation and cell death. B17 inhibits type II topoisomerase by trapping an enzyme - DNA cleavable complex.1 Publication

GO - Biological processi

Keywordsi

Molecular functionAntibiotic, Antimicrobial, Bacteriocin, DNA replication inhibitor

Names & Taxonomyi

Protein namesi
Recommended name:
Bacteriocin microcin B17
Short name:
MccB17
Gene namesi
Name:mcbA
Encoded oniPlasmid IncFII pMccB170 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00000027721 – 262 PublicationsAdd BLAST26
ChainiPRO_000000277327 – 69Bacteriocin microcin B17Add BLAST43

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki39 ↔ 40Oxazole-4-carboxylic acid (Gly-Ser)1 Publication
Cross-linki40 ↔ 41Thiazole-4-carboxylic acid (Ser-Cys)1 Publication
Cross-linki47 ↔ 48Thiazole-4-carboxylic acid (Gly-Cys)1 Publication
Cross-linki50 ↔ 51Thiazole-4-carboxylic acid (Gly-Cys)1 Publication
Cross-linki54 ↔ 55Thiazole-4-carboxylic acid (Gly-Cys)1 Publication
Cross-linki55 ↔ 56Oxazole-4-carboxylic acid (Cys-Ser)1 Publication
Cross-linki61 ↔ 62Oxazole-4-carboxylic acid (Gly-Ser)1 Publication
Cross-linki64 ↔ 65Oxazole-4-carboxylic acid (Gly-Ser)1 Publication

Post-translational modificationi

The processed N-terminus does not resemble a typical secretion signal sequence.
Maturation of thiazole and oxazole containing antibiotics involves the enzymic condensation of a Cys, Ser or Thr with the alpha-carbonyl of the preceding amino acid to form a thioether or ether bond, then dehydration to form a double bond with the alpha-amino nitrogen. Thiazoline or oxazoline rings are dehydrogenated to form thiazole or oxazole rings.

Keywords - PTMi

Thioether bond

Proteomic databases

PRIDEiP05834

Miscellaneous databases

PMAP-CutDBiP05834

Structurei

Secondary structure

169
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi8 – 15Combined sources8
Helixi18 – 21Combined sources4
Beta strandi22 – 24Combined sources3

3D structure databases

ProteinModelPortaliP05834
SMRiP05834
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP05834

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi26 – 39Poly-GlyAdd BLAST14

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P05834-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELKASEFGV VLSVDALKLS RQSPLGVGIG GGGGGGGGGS CGGQGGGCGG
60
CSNGCSGGNG GSGGSGSHI
Length:69
Mass (Da):6,013
Last modified:November 1, 1988 - v1
Checksum:i0B1D159A832638A8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15469 Genomic DNA Translation: AAA24141.1
M24253 Genomic DNA Translation: AAA72741.1
X06417 Genomic DNA Translation: CAA29725.1
PIRiA25219 MIEC77
A58375
RefSeqiWP_001535752.1, NZ_PGJZ01000055.1

Similar proteinsi

Entry informationi

Entry nameiMCBA_ECOLX
AccessioniPrimary (citable) accession number: P05834
Secondary accession number(s): Q7M024
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: July 18, 2018
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health