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Protein

Beta-glucuronidase

Gene

uidA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Substrates for this enzyme are generally water-soluble.

Catalytic activityi

A beta-D-glucuronoside + H2O = D-glucuronate + an alcohol.

pH dependencei

Optimum pH is 5.0-7.5.

Temperature dependencei

Resistant to thermal inactivation at 50 degrees Celsius.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei413Proton donorBy similarity1

GO - Molecular functioni

  • beta-glucuronidase activity Source: EcoCyc
  • carbohydrate binding Source: GO_Central
  • identical protein binding Source: EcoCyc

GO - Biological processi

  • glucuronoside catabolic process Source: EcoCyc
  • protein homotetramerization Source: EcoCyc

Keywordsi

Molecular functionGlycosidase, Hydrolase

Enzyme and pathway databases

BioCyciEcoCyc:BETA-GLUCURONID-MONOMER
MetaCyc:BETA-GLUCURONID-MONOMER
BRENDAi3.2.1.31 2026
SABIO-RKiP05804

Protein family/group databases

CAZyiGH2 Glycoside Hydrolase Family 2

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-glucuronidase (EC:3.2.1.31)
Short name:
GUS
Alternative name(s):
Beta-D-glucuronoside glucuronosohydrolase
Gene namesi
Name:uidA
Synonyms:gurA, gusA
Ordered Locus Names:b1617, JW1609
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11055 uidA

Subcellular locationi

GO - Cellular componenti

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3217380

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000576801 – 603Beta-glucuronidaseAdd BLAST603

Proteomic databases

PaxDbiP05804
PRIDEiP05804

Interactioni

Subunit structurei

Homotetramer.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi4263483, 12 interactors
DIPiDIP-11086N
IntActiP05804, 2 interactors
STRINGi316385.ECDH10B_1750

Chemistry databases

BindingDBiP05804

Structurei

Secondary structure

1603
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP05804
SMRiP05804
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP05804

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.Curated

Phylogenomic databases

eggNOGiENOG4105CNT Bacteria
COG3250 LUCA
HOGENOMiHOG000120896
InParanoidiP05804
KOiK01195
PhylomeDBiP05804

Family and domain databases

Gene3Di2.60.120.260, 1 hit
2.60.40.10, 1 hit
InterProiView protein in InterPro
IPR036156 Beta-gal/glucu_dom_sf
IPR008979 Galactose-bd-like_sf
IPR006101 Glyco_hydro_2
IPR023232 Glyco_hydro_2_AS
IPR006103 Glyco_hydro_2_cat
IPR023230 Glyco_hydro_2_CS
IPR006102 Glyco_hydro_2_Ig-like
IPR006104 Glyco_hydro_2_N
IPR017853 Glycoside_hydrolase_SF
IPR013783 Ig-like_fold
PfamiView protein in Pfam
PF00703 Glyco_hydro_2, 1 hit
PF02836 Glyco_hydro_2_C, 1 hit
PF02837 Glyco_hydro_2_N, 1 hit
PRINTSiPR00132 GLHYDRLASE2
SUPFAMiSSF49303 SSF49303, 1 hit
SSF49785 SSF49785, 1 hit
SSF51445 SSF51445, 1 hit
PROSITEiView protein in PROSITE
PS00719 GLYCOSYL_HYDROL_F2_1, 1 hit
PS00608 GLYCOSYL_HYDROL_F2_2, 1 hit

Sequencei

Sequence statusi: Complete.

P05804-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLRPVETPTR EIKKLDGLWA FSLDRENCGI DQRWWESALQ ESRAIAVPGS
60 70 80 90 100
FNDQFADADI RNYAGNVWYQ REVFIPKGWA GQRIVLRFDA VTHYGKVWVN
110 120 130 140 150
NQEVMEHQGG YTPFEADVTP YVIAGKSVRI TVCVNNELNW QTIPPGMVIT
160 170 180 190 200
DENGKKKQSY FHDFFNYAGI HRSVMLYTTP NTWVDDITVV THVAQDCNHA
210 220 230 240 250
SVDWQVVANG DVSVELRDAD QQVVATGQGT SGTLQVVNPH LWQPGEGYLY
260 270 280 290 300
ELCVTAKSQT ECDIYPLRVG IRSVAVKGEQ FLINHKPFYF TGFGRHEDAD
310 320 330 340 350
LRGKGFDNVL MVHDHALMDW IGANSYRTSH YPYAEEMLDW ADEHGIVVID
360 370 380 390 400
ETAAVGFNLS LGIGFEAGNK PKELYSEEAV NGETQQAHLQ AIKELIARDK
410 420 430 440 450
NHPSVVMWSI ANEPDTRPQG AREYFAPLAE ATRKLDPTRP ITCVNVMFCD
460 470 480 490 500
AHTDTISDLF DVLCLNRYYG WYVQSGDLET AEKVLEKELL AWQEKLHQPI
510 520 530 540 550
IITEYGVDTL AGLHSMYTDM WSEEYQCAWL DMYHRVFDRV SAVVGEQVWN
560 570 580 590 600
FADFATSQGI LRVGGNKKGI FTRDRKPKSA AFLLQKRWTG MNFGEKPQQG

GKQ
Length:603
Mass (Da):68,447
Last modified:October 1, 1994 - v2
Checksum:iE769C8D61A3B9A76
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14641 Genomic DNA Translation: AAA68923.1 Sequence problems.
S69414 Genomic DNA Translation: AAB30197.1
U00096 Genomic DNA Translation: AAC74689.1
AP009048 Genomic DNA Translation: BAA15368.1
PIRiC64918 GBECGC
RefSeqiNP_416134.1, NC_000913.3
WP_000945878.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74689; AAC74689; b1617
BAA15368; BAA15368; BAA15368
GeneIDi946149
KEGGiecj:JW1609
eco:b1617
PATRICifig|1411691.4.peg.644

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14641 Genomic DNA Translation: AAA68923.1 Sequence problems.
S69414 Genomic DNA Translation: AAB30197.1
U00096 Genomic DNA Translation: AAC74689.1
AP009048 Genomic DNA Translation: BAA15368.1
PIRiC64918 GBECGC
RefSeqiNP_416134.1, NC_000913.3
WP_000945878.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3K46X-ray2.50A/B1-603[»]
3K4AX-ray2.90A/B1-603[»]
3K4DX-ray2.39A/B1-603[»]
3LPFX-ray2.26A/B1-603[»]
3LPGX-ray2.42A/B1-603[»]
4JHZX-ray2.83A/B1-601[»]
5CZKX-ray2.39A/B1-603[»]
ProteinModelPortaliP05804
SMRiP05804
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263483, 12 interactors
DIPiDIP-11086N
IntActiP05804, 2 interactors
STRINGi316385.ECDH10B_1750

Chemistry databases

BindingDBiP05804
ChEMBLiCHEMBL3217380

Protein family/group databases

CAZyiGH2 Glycoside Hydrolase Family 2

Proteomic databases

PaxDbiP05804
PRIDEiP05804

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74689; AAC74689; b1617
BAA15368; BAA15368; BAA15368
GeneIDi946149
KEGGiecj:JW1609
eco:b1617
PATRICifig|1411691.4.peg.644

Organism-specific databases

EchoBASEiEB1048
EcoGeneiEG11055 uidA

Phylogenomic databases

eggNOGiENOG4105CNT Bacteria
COG3250 LUCA
HOGENOMiHOG000120896
InParanoidiP05804
KOiK01195
PhylomeDBiP05804

Enzyme and pathway databases

BioCyciEcoCyc:BETA-GLUCURONID-MONOMER
MetaCyc:BETA-GLUCURONID-MONOMER
BRENDAi3.2.1.31 2026
SABIO-RKiP05804

Miscellaneous databases

EvolutionaryTraceiP05804
PROiPR:P05804

Family and domain databases

Gene3Di2.60.120.260, 1 hit
2.60.40.10, 1 hit
InterProiView protein in InterPro
IPR036156 Beta-gal/glucu_dom_sf
IPR008979 Galactose-bd-like_sf
IPR006101 Glyco_hydro_2
IPR023232 Glyco_hydro_2_AS
IPR006103 Glyco_hydro_2_cat
IPR023230 Glyco_hydro_2_CS
IPR006102 Glyco_hydro_2_Ig-like
IPR006104 Glyco_hydro_2_N
IPR017853 Glycoside_hydrolase_SF
IPR013783 Ig-like_fold
PfamiView protein in Pfam
PF00703 Glyco_hydro_2, 1 hit
PF02836 Glyco_hydro_2_C, 1 hit
PF02837 Glyco_hydro_2_N, 1 hit
PRINTSiPR00132 GLHYDRLASE2
SUPFAMiSSF49303 SSF49303, 1 hit
SSF49785 SSF49785, 1 hit
SSF51445 SSF51445, 1 hit
PROSITEiView protein in PROSITE
PS00719 GLYCOSYL_HYDROL_F2_1, 1 hit
PS00608 GLYCOSYL_HYDROL_F2_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiBGLR_ECOLI
AccessioniPrimary (citable) accession number: P05804
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: October 1, 1994
Last modified: November 7, 2018
This is version 152 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  4. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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