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Protein

Guanyl-specific ribonuclease Sa

Gene

rnaSA

Organism
Kitasatospora aureofaciens (Streptomyces aureofaciens)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

Two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in G-P with 2',3'-cyclic phosphate intermediates.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei54Proton acceptor1
Active sitei85Proton donor1

GO - Molecular functioni

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease

Enzyme and pathway databases

BRENDAi3.1.27.3 5978

Names & Taxonomyi

Protein namesi
Recommended name:
Guanyl-specific ribonuclease Sa (EC:3.1.27.3)
Short name:
RNase Sa
Gene namesi
Name:rnaSA
OrganismiKitasatospora aureofaciens (Streptomyces aureofaciens)
Taxonomic identifieri1894 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeKitasatospora

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi39N → A, D or S: Decreases protein stability. 1

Chemistry databases

DrugBankiDB01937 Guanosine-2'-Monophosphate

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001373671 – 96Guanyl-specific ribonuclease SaAdd BLAST96

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi7 ↔ 96

Keywords - PTMi

Disulfide bond

Interactioni

Protein-protein interaction databases

IntActiP05798, 1 interactor

Structurei

Secondary structure

196
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP05798
SMRiP05798
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP05798

Family & Domainsi

Sequence similaritiesi

Belongs to the ribonuclease N1/T1 family.Curated

Family and domain databases

InterProiView protein in InterPro
IPR000026 Gua-sp_ribonuclease_N1/T1/U2
IPR016191 Ribonuclease/ribotoxin
PfamiView protein in Pfam
PF00545 Ribonuclease, 1 hit
SUPFAMiSSF53933 SSF53933, 1 hit

Sequencei

Sequence statusi: Complete.

P05798-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
DVSGTVCLSA LPPEATDTLN LIASDGPFPY SQDGVVFQNR ESVLPTQSYG
60 70 80 90
YYHEYTVITP GARTRGTRRI ITGEATQEDY YTGDHYATFS LIDQTC
Length:96
Mass (Da):10,576
Last modified:April 27, 2001 - v2
Checksum:iFA4DB2FD0BA8A7E4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti72T → C AA sequence (PubMed:3098582).Curated1
Sequence conflicti72T → C AA sequence (PubMed:3118883).Curated1

Sequence databases

PIRiA25655 NRSM

Similar proteinsi

Cross-referencesi

Sequence databases

PIRiA25655 NRSM

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AY7X-ray1.70A1-96[»]
1BOXX-ray1.60A1-96[»]
1C54NMR-A1-96[»]
1GMPX-ray1.70A/B1-96[»]
1GMQX-ray1.80A/B1-96[»]
1GMRX-ray1.77A/B1-96[»]
1I70X-ray1.70A/B1-96[»]
1I8VX-ray1.25A/B1-96[»]
1LNIX-ray1.00A/B1-96[»]
1RGEX-ray1.15A/B1-96[»]
1RGFX-ray1.20A/B1-96[»]
1RGGX-ray1.20A/B1-96[»]
1RGHX-ray1.20A/B1-96[»]
1RSNX-ray2.00A/B1-96[»]
1SARX-ray1.80A/B1-96[»]
1T2HX-ray1.00A/B1-96[»]
1T2IX-ray1.10A1-96[»]
1UCIX-ray1.80A/B1-96[»]
1UCJX-ray1.81A/B1-96[»]
1UCKX-ray1.80A/B1-96[»]
1UCLX-ray1.82A/B1-96[»]
1YNVX-ray1.20X1-96[»]
1ZGXX-ray1.13A1-63[»]
B64-96[»]
2SARX-ray1.80A/B1-96[»]
3A5EX-ray1.60A1-96[»]
4GHOX-ray1.10A/B1-96[»]
4J5GX-ray1.31A/B1-94[»]
4J5KX-ray1.23A/B1-96[»]
ProteinModelPortaliP05798
SMRiP05798
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP05798, 1 interactor

Chemistry databases

DrugBankiDB01937 Guanosine-2'-Monophosphate

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi3.1.27.3 5978

Miscellaneous databases

EvolutionaryTraceiP05798

Family and domain databases

InterProiView protein in InterPro
IPR000026 Gua-sp_ribonuclease_N1/T1/U2
IPR016191 Ribonuclease/ribotoxin
PfamiView protein in Pfam
PF00545 Ribonuclease, 1 hit
SUPFAMiSSF53933 SSF53933, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRNSA_KITAU
AccessioniPrimary (citable) accession number: P05798
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: April 27, 2001
Last modified: February 28, 2018
This is version 112 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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