UniProtKB - P05791 (ILVD_ECOLI)
Protein
Dihydroxy-acid dehydratase
Gene
ilvD
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalytic activityi
- EC:4.2.1.9UniRule annotation2 Publications
Cofactori
[4Fe-4S] clusterUniRule annotation2 PublicationsNote: Binds 1 [4Fe-4S] cluster.UniRule annotation2 Publications
: L-isoleucine biosynthesis Pathwayi
This protein is involved in step 3 of the subpathway that synthesizes L-isoleucine from 2-oxobutanoate.UniRule annotation1 PublicationProteins known to be involved in the 4 steps of the subpathway in this organism are:
- Acetolactate synthase isozyme 1 small subunit (ilvN), Putative acetolactate synthase isozyme 2 large subunit (ilvG), Acetolactate synthase isozyme 1 large subunit (ilvB), Acetolactate synthase (ilvB), Acetolactate synthase (FAZ83_14160), Acetolactate synthase (ilvI), Acetolactate synthase isozyme 2 small subunit (ilvM), Acetolactate synthase isozyme 2 large subunit (ilvG), Acetolactate synthase isozyme 3 large subunit (ilvI), Acetolactate synthase (ilvN), Acetolactate synthase isozyme 3 small subunit (ilvH), Acetolactate synthase (ilvN)
- Ketol-acid reductoisomerase (NADP(+)) (ilvC), Ketol-acid reductoisomerase (NADP(+)) (ilvC)
- Dihydroxy-acid dehydratase (ilvD), Dihydroxy-acid dehydratase (ilvD)
- Branched-chain-amino-acid aminotransferase (ilvE), Branched-chain-amino-acid aminotransferase (ilvE)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-isoleucine from 2-oxobutanoate, the pathway L-isoleucine biosynthesis and in Amino-acid biosynthesis.
Pathwayi: L-valine biosynthesis
This protein is involved in step 3 of the subpathway that synthesizes L-valine from pyruvate.UniRule annotation1 PublicationProteins known to be involved in the 4 steps of the subpathway in this organism are:
- Acetolactate synthase isozyme 1 small subunit (ilvN), Putative acetolactate synthase isozyme 2 large subunit (ilvG), Acetolactate synthase isozyme 1 large subunit (ilvB), Acetolactate synthase (ilvB), Acetolactate synthase (FAZ83_14160), Acetolactate synthase (ilvI), Acetolactate synthase isozyme 2 small subunit (ilvM), Acetolactate synthase isozyme 2 large subunit (ilvG), Acetolactate synthase isozyme 3 large subunit (ilvI), Acetolactate synthase (ilvN), Acetolactate synthase isozyme 3 small subunit (ilvH), Acetolactate synthase (ilvN)
- Ketol-acid reductoisomerase (NADP(+)) (ilvC), Ketol-acid reductoisomerase (NADP(+)) (ilvC)
- Dihydroxy-acid dehydratase (ilvD), Dihydroxy-acid dehydratase (ilvD)
- Branched-chain-amino-acid aminotransferase (ilvE), Branched-chain-amino-acid aminotransferase (ilvE)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-valine from pyruvate, the pathway L-valine biosynthesis and in Amino-acid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 122 | Iron-sulfur (4Fe-4S)UniRule annotation | 1 | |
Metal bindingi | 195 | Iron-sulfur (4Fe-4S)UniRule annotation | 1 |
GO - Molecular functioni
- 4 iron, 4 sulfur cluster binding Source: EcoCyc
- dihydroxy-acid dehydratase activity Source: EcoCyc
- hydro-lyase activity Source: GO_Central
- iron-sulfur cluster binding Source: EcoCyc
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- isoleucine biosynthetic process Source: EcoCyc
- valine biosynthetic process Source: EcoCyc
Keywordsi
Molecular function | Lyase |
Biological process | Amino-acid biosynthesis, Branched-chain amino acid biosynthesis |
Ligand | 4Fe-4S, Iron, Iron-sulfur, Metal-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:DIHYDROXYACIDDEHYDRAT-MONOMER MetaCyc:DIHYDROXYACIDDEHYDRAT-MONOMER |
UniPathwayi | UPA00047;UER00057 UPA00049;UER00061 |
Names & Taxonomyi
Protein namesi | Recommended name: Dihydroxy-acid dehydrataseUniRule annotation (EC:4.2.1.9UniRule annotation)Short name: DADUniRule annotation |
Gene namesi | Name:ilvDUniRule annotation Ordered Locus Names:b3771, JW5605 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000103465 | 2 – 616 | Dihydroxy-acid dehydrataseAdd BLAST | 615 |
Proteomic databases
jPOSTi | P05791 |
PaxDbi | P05791 |
PRIDEi | P05791 |
2D gel databases
SWISS-2DPAGEi | P05791 |
Interactioni
Subunit structurei
Homodimer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4263329, 5 interactors |
DIPi | DIP-10021N |
IntActi | P05791, 1 interactor |
STRINGi | 511145.b3771 |
Family & Domainsi
Sequence similaritiesi
Belongs to the IlvD/Edd family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0129, Bacteria |
HOGENOMi | CLU_014271_4_2_6 |
InParanoidi | P05791 |
PhylomeDBi | P05791 |
Family and domain databases
Gene3Di | 3.50.30.80, 1 hit |
HAMAPi | MF_00012, IlvD, 1 hit |
InterProi | View protein in InterPro IPR042096, Dihydro-acid_dehy_C IPR004404, DihydroxyA_deHydtase IPR000581, DiOHA_6PGluconate_deHydtase IPR020558, DiOHA_6PGluconate_deHydtase_CS IPR037237, IlvD/EDD_N |
Pfami | View protein in Pfam PF00920, ILVD_EDD, 1 hit |
SUPFAMi | SSF143975, SSF143975, 1 hit |
TIGRFAMsi | TIGR00110, ilvD, 1 hit |
PROSITEi | View protein in PROSITE PS00886, ILVD_EDD_1, 1 hit PS00887, ILVD_EDD_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P05791-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPKYRSATTT HGRNMAGARA LWRATGMTDA DFGKPIIAVV NSFTQFVPGH
60 70 80 90 100
VHLRDLGKLV AEQIEAAGGV AKEFNTIAVD DGIAMGHGGM LYSLPSRELI
110 120 130 140 150
ADSVEYMVNA HCADAMVCIS NCDKITPGML MASLRLNIPV IFVSGGPMEA
160 170 180 190 200
GKTKLSDQII KLDLVDAMIQ GADPKVSDSQ SDQVERSACP TCGSCSGMFT
210 220 230 240 250
ANSMNCLTEA LGLSQPGNGS LLATHADRKQ LFLNAGKRIV ELTKRYYEQN
260 270 280 290 300
DESALPRNIA SKAAFENAMT LDIAMGGSTN TVLHLLAAAQ EAEIDFTMSD
310 320 330 340 350
IDKLSRKVPQ LCKVAPSTQK YHMEDVHRAG GVIGILGELD RAGLLNRDVK
360 370 380 390 400
NVLGLTLPQT LEQYDVMLTQ DDAVKNMFRA GPAGIRTTQA FSQDCRWDTL
410 420 430 440 450
DDDRANGCIR SLEHAYSKDG GLAVLYGNFA ENGCIVKTAG VDDSILKFTG
460 470 480 490 500
PAKVYESQDD AVEAILGGKV VAGDVVVIRY EGPKGGPGMQ EMLYPTSFLK
510 520 530 540 550
SMGLGKACAL ITDGRFSGGT SGLSIGHVSP EAASGGSIGL IEDGDLIAID
560 570 580 590 600
IPNRGIQLQV SDAELAARRE AQDARGDKAW TPKNRERQVS FALRAYASLA
610
TSADKGAVRD KSKLGG
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 2 – 24 | PKYRS…ALWRA → VVIWRVLVRCGP in AAA24023 (PubMed:3550695).CuratedAdd BLAST | 23 | |
Sequence conflicti | 164 | L → P in AAA24023 (PubMed:3550695).Curated | 1 | |
Sequence conflicti | 408 – 437 | CIRSL…GCIVK → VSARWNTPTAKTAAWRCSTV ILRKRLHRE in AAA24023 (PubMed:3550695).CuratedAdd BLAST | 30 | |
Sequence conflicti | 610 | D → H in AAA24023 (PubMed:3550695).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X04890 Genomic DNA Translation: CAA28576.1 M10313 Genomic DNA Translation: AAB59053.1 M87049 Genomic DNA Translation: AAA67574.1 U00096 Genomic DNA Translation: AAT48208.1 AP009048 Genomic DNA Translation: BAE77526.1 M32253 Genomic DNA Translation: AAA24023.1 X02413 Genomic DNA Translation: CAA26263.1 K03503 Genomic DNA Translation: AAA24013.1 |
PIRi | A27310, DWECDA |
RefSeqi | WP_001127399.1, NZ_SSZK01000025.1 YP_026248.1, NC_000913.3 |
Genome annotation databases
EnsemblBacteriai | AAT48208; AAT48208; b3771 BAE77526; BAE77526; BAE77526 |
GeneIDi | 948277 |
KEGGi | ecj:JW5605 eco:b3771 |
PATRICi | fig|1411691.4.peg.2935 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X04890 Genomic DNA Translation: CAA28576.1 M10313 Genomic DNA Translation: AAB59053.1 M87049 Genomic DNA Translation: AAA67574.1 U00096 Genomic DNA Translation: AAT48208.1 AP009048 Genomic DNA Translation: BAE77526.1 M32253 Genomic DNA Translation: AAA24023.1 X02413 Genomic DNA Translation: CAA26263.1 K03503 Genomic DNA Translation: AAA24013.1 |
PIRi | A27310, DWECDA |
RefSeqi | WP_001127399.1, NZ_SSZK01000025.1 YP_026248.1, NC_000913.3 |
3D structure databases
SMRi | P05791 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4263329, 5 interactors |
DIPi | DIP-10021N |
IntActi | P05791, 1 interactor |
STRINGi | 511145.b3771 |
2D gel databases
SWISS-2DPAGEi | P05791 |
Proteomic databases
jPOSTi | P05791 |
PaxDbi | P05791 |
PRIDEi | P05791 |
Genome annotation databases
EnsemblBacteriai | AAT48208; AAT48208; b3771 BAE77526; BAE77526; BAE77526 |
GeneIDi | 948277 |
KEGGi | ecj:JW5605 eco:b3771 |
PATRICi | fig|1411691.4.peg.2935 |
Organism-specific databases
EchoBASEi | EB0491 |
Phylogenomic databases
eggNOGi | COG0129, Bacteria |
HOGENOMi | CLU_014271_4_2_6 |
InParanoidi | P05791 |
PhylomeDBi | P05791 |
Enzyme and pathway databases
UniPathwayi | UPA00047;UER00057 UPA00049;UER00061 |
BioCyci | EcoCyc:DIHYDROXYACIDDEHYDRAT-MONOMER MetaCyc:DIHYDROXYACIDDEHYDRAT-MONOMER |
Miscellaneous databases
PROi | PR:P05791 |
Family and domain databases
Gene3Di | 3.50.30.80, 1 hit |
HAMAPi | MF_00012, IlvD, 1 hit |
InterProi | View protein in InterPro IPR042096, Dihydro-acid_dehy_C IPR004404, DihydroxyA_deHydtase IPR000581, DiOHA_6PGluconate_deHydtase IPR020558, DiOHA_6PGluconate_deHydtase_CS IPR037237, IlvD/EDD_N |
Pfami | View protein in Pfam PF00920, ILVD_EDD, 1 hit |
SUPFAMi | SSF143975, SSF143975, 1 hit |
TIGRFAMsi | TIGR00110, ilvD, 1 hit |
PROSITEi | View protein in PROSITE PS00886, ILVD_EDD_1, 1 hit PS00887, ILVD_EDD_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ILVD_ECOLI | |
Accessioni | P05791Primary (citable) accession number: P05791 Secondary accession number(s): Q2M880 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1988 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 188 of the entry and version 5 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families