UniProtKB - P05653 (GYRA_BACSU)
Protein
DNA gyrase subunit A
Gene
gyrA
Organism
Bacillus subtilis (strain 168)
Status
Functioni
A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.UniRule annotation
Miscellaneous
Few gyrases are as efficient as E.coli at forming negative supercoils. Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes.UniRule annotation
Catalytic activityi
- ATP-dependent breakage, passage and rejoining of double-stranded DNA.UniRule annotation EC:5.6.2.2
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 123 | O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation | 1 |
GO - Molecular functioni
- ATP binding Source: GO_Central
- DNA binding Source: GO_Central
- DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity Source: UniProtKB-UniRule
GO - Biological processi
- DNA-dependent DNA replication Source: UniProtKB-UniRule
- DNA topological change Source: GO_Central
Keywordsi
Molecular function | DNA-binding, Isomerase, Topoisomerase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | BSUB:BSU00070-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:gyrAUniRule annotation Synonyms:cafB, nalA Ordered Locus Names:BSU00070 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm UniRule annotation
GO - Cellular componenti
- chromosome Source: InterPro
- cytoplasm Source: GO_Central
- DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex Source: GO_Central
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000145221 | 1 – 821 | DNA gyrase subunit AAdd BLAST | 821 |
Proteomic databases
jPOSTi | P05653 |
PaxDbi | P05653 |
PRIDEi | P05653 |
Interactioni
Subunit structurei
Heterotetramer, composed of two GyrA and two GyrB chains. In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis.
UniRule annotationProtein-protein interaction databases
IntActi | P05653, 5 interactors |
MINTi | P05653 |
STRINGi | 224308.BSU00070 |
Chemistry databases
BindingDBi | P05653 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P05653 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 527 – 533 | GyrA-boxUniRule annotation | 7 |
Sequence similaritiesi
Belongs to the type II topoisomerase GyrA/ParC subunit family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0188, Bacteria |
InParanoidi | P05653 |
OMAi | THHWLLF |
PhylomeDBi | P05653 |
Family and domain databases
CDDi | cd00187, TOP4c, 1 hit |
Gene3Di | 1.10.268.10, 1 hit 2.120.10.90, 1 hit 3.90.199.10, 1 hit |
HAMAPi | MF_01897, GyrA, 1 hit |
InterProi | View protein in InterPro IPR005743, GyrA IPR006691, GyrA/parC_rep IPR035516, Gyrase/topoIV_suA_C IPR013760, Topo_IIA-like_dom_sf IPR002205, Topo_IIA_A/C IPR013758, Topo_IIA_A/C_ab IPR013757, Topo_IIA_A_a_sf |
Pfami | View protein in Pfam PF03989, DNA_gyraseA_C, 6 hits PF00521, DNA_topoisoIV, 1 hit |
SMARTi | View protein in SMART SM00434, TOP4c, 1 hit |
SUPFAMi | SSF101904, SSF101904, 1 hit SSF56719, SSF56719, 1 hit |
i Sequence
Sequence statusi: Complete.
P05653-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSEQNTPQVR EINISQEMRT SFLDYAMSVI VSRALPDVRD GLKPVHRRIL
60 70 80 90 100
YAMNDLGMTS DKPYKKSARI VGEVIGKYHP HGDSAVYESM VRMAQDFNYR
110 120 130 140 150
YMLVDGHGNF GSVDGDSAAA MRYTEARMSK ISMEILRDIT KDTIDYQDNY
160 170 180 190 200
DGSEREPVVM PSRFPNLLVN GAAGIAVGMA TNIPPHQLGE IIDGVLAVSE
210 220 230 240 250
NPDITIPELM EVIPGPDFPT AGQILGRSGI RKAYESGRGS ITIRAKAEIE
260 270 280 290 300
QTSSGKERII VTELPYQVNK AKLIEKIADL VRDKKIEGIT DLRDESDRTG
310 320 330 340 350
MRIVIEIRRD ANANVILNNL YKQTALQTSF GINLLALVDG QPKVLTLKQC
360 370 380 390 400
LEHYLDHQKV VIRRRTAYEL RKAEARAHIL EGLRVALDHL DAVISLIRNS
410 420 430 440 450
QTAEIARTGL IEQFSLTEKQ AQAILDMRLQ RLTGLEREKI EEEYQSLVKL
460 470 480 490 500
IAELKDILAN EYKVLEIIRE ELTEIKERFN DERRTEIVTS GLETIEDEDL
510 520 530 540 550
IERENIVVTL THNGYVKRLP ASTYRSQKRG GKGVQGMGTN EDDFVEHLIS
560 570 580 590 600
TSTHDTILFF SNKGKVYRAK GYEIPEYGRT AKGIPIINLL EVEKGEWINA
610 620 630 640 650
IIPVTEFNAE LYLFFTTKHG VSKRTSLSQF ANIRNNGLIA LSLREDDELM
660 670 680 690 700
GVRLTDGTKQ IIIGTKNGLL IRFPETDVRE MGRTAAGVKG ITLTDDDVVV
710 720 730 740 750
GMEILEEESH VLIVTEKGYG KRTPAEEYRT QSRGGKGLKT AKITENNGQL
760 770 780 790 800
VAVKATKGEE DLMIITASGV LIRMDINDIS ITGRVTQGVR LIRMAEEEHV
810 820
ATVALVEKNE EDENEEEQEE V
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X02369 Genomic DNA Translation: CAA26222.1 D26185 Genomic DNA Translation: BAA05243.1 AL009126 Genomic DNA Translation: CAB11783.1 |
PIRi | F22930 |
RefSeqi | NP_387888.1, NC_000964.3 WP_003244540.1, NZ_JNCM01000034.1 |
Genome annotation databases
EnsemblBacteriai | CAB11783; CAB11783; BSU00070 |
GeneIDi | 51993863 940002 |
KEGGi | bsu:BSU00070 |
PATRICi | fig|224308.179.peg.7 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X02369 Genomic DNA Translation: CAA26222.1 D26185 Genomic DNA Translation: BAA05243.1 AL009126 Genomic DNA Translation: CAB11783.1 |
PIRi | F22930 |
RefSeqi | NP_387888.1, NC_000964.3 WP_003244540.1, NZ_JNCM01000034.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4DDQ | X-ray | 3.30 | A/B/C/D/E/F | 1-502 | [»] | |
SMRi | P05653 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | P05653, 5 interactors |
MINTi | P05653 |
STRINGi | 224308.BSU00070 |
Chemistry databases
BindingDBi | P05653 |
DrugBanki | DB00537, Ciprofloxacin |
Proteomic databases
jPOSTi | P05653 |
PaxDbi | P05653 |
PRIDEi | P05653 |
Genome annotation databases
EnsemblBacteriai | CAB11783; CAB11783; BSU00070 |
GeneIDi | 51993863 940002 |
KEGGi | bsu:BSU00070 |
PATRICi | fig|224308.179.peg.7 |
Phylogenomic databases
eggNOGi | COG0188, Bacteria |
InParanoidi | P05653 |
OMAi | THHWLLF |
PhylomeDBi | P05653 |
Enzyme and pathway databases
BioCyci | BSUB:BSU00070-MONOMER |
Family and domain databases
CDDi | cd00187, TOP4c, 1 hit |
Gene3Di | 1.10.268.10, 1 hit 2.120.10.90, 1 hit 3.90.199.10, 1 hit |
HAMAPi | MF_01897, GyrA, 1 hit |
InterProi | View protein in InterPro IPR005743, GyrA IPR006691, GyrA/parC_rep IPR035516, Gyrase/topoIV_suA_C IPR013760, Topo_IIA-like_dom_sf IPR002205, Topo_IIA_A/C IPR013758, Topo_IIA_A/C_ab IPR013757, Topo_IIA_A_a_sf |
Pfami | View protein in Pfam PF03989, DNA_gyraseA_C, 6 hits PF00521, DNA_topoisoIV, 1 hit |
SMARTi | View protein in SMART SM00434, TOP4c, 1 hit |
SUPFAMi | SSF101904, SSF101904, 1 hit SSF56719, SSF56719, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GYRA_BACSU | |
Accessioni | P05653Primary (citable) accession number: P05653 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1988 |
Last sequence update: | November 1, 1988 | |
Last modified: | December 2, 2020 | |
This is version 152 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families