Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Cyclomaltodextrin glucanotransferase

Gene

cgt

Organism
Bacillus sp. (strain 1011)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

Cyclizes part of a (1->4)-alpha-D-glucan chain by formation of a (1->4)-alpha-D-glucosidic bond.

Cofactori

Ca2+Note: Binds 2 calcium ions per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi54Calcium 11
Metal bindingi56Calcium 1; via carbonyl oxygen1
Metal bindingi59Calcium 11
Metal bindingi60Calcium 11
Metal bindingi78Calcium 1; via carbonyl oxygen1
Metal bindingi80Calcium 11
Metal bindingi166Calcium 21
Binding sitei167SubstrateBy similarity1
Metal bindingi217Calcium 2; via carbonyl oxygen1
Metal bindingi226Calcium 21
Binding sitei254SubstrateBy similarity1
Active sitei256Nucleophile1
Metal bindingi260Calcium 2; via carbonyl oxygen1
Active sitei284Proton donor1
Binding sitei354SubstrateBy similarity1
Sitei355Transition state stabilizerBy similarity1
Binding sitei398Substrate1
Binding sitei402Substrate1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
LigandCalcium, Metal-binding

Enzyme and pathway databases

BRENDAi2.4.1.19 691

Protein family/group databases

CAZyiCBM20 Carbohydrate-Binding Module Family 20
GH13 Glycoside Hydrolase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclomaltodextrin glucanotransferase (EC:2.4.1.19)
Alternative name(s):
Cyclodextrin-glycosyltransferase
Short name:
CGTase
Gene namesi
Name:cgt
OrganismiBacillus sp. (strain 1011)
Taxonomic identifieri1410 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Chemistry databases

DrugBankiDB02379 Beta-D-Glucose
DB03206 Duvoglustat

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Add BLAST27
ChainiPRO_000000143728 – 713Cyclomaltodextrin glucanotransferaseAdd BLAST686

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi70 ↔ 77

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP05618

Interactioni

Subunit structurei

Monomer.

Structurei

Secondary structure

1713
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP05618
SMRiP05618
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP05618

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini526 – 607IPT/TIGAdd BLAST82
Domaini608 – 713CBM20PROSITE-ProRule annotationAdd BLAST106

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni28 – 165A1Add BLAST138
Regioni127 – 128Substrate binding2
Regioni166 – 229BAdd BLAST64
Regioni220 – 223Substrate binding4
Regioni230 – 433A2Add BLAST204
Regioni259 – 260Substrate bindingBy similarity2
Regioni434 – 522CAdd BLAST89
Regioni523 – 609DAdd BLAST87
Regioni610 – 713EAdd BLAST104

Domaini

May consist of two protein domains: the one in the N-terminal side cleaves the alpha-1,4-glucosidic bond in starch, and the other in the C-terminal side catalyzes other activities, including the reconstitution of an alpha-1,4-glucosidic linkage for cyclizing the maltooligosaccharide produced.

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.40.10, 2 hits
2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR006048 A-amylase/branching_C
IPR031319 A-amylase_C
IPR006046 Alpha_amylase
IPR013784 Carb-bd-like_fold
IPR002044 CBM_fam20
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
IPR013783 Ig-like_fold
IPR014756 Ig_E-set
IPR002909 IPT_dom
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF02806 Alpha-amylase_C, 1 hit
PF00686 CBM_20, 1 hit
PF01833 TIG, 1 hit
PRINTSiPR00110 ALPHAAMYLASE
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SM00632 Aamy_C, 1 hit
SM01065 CBM_2, 1 hit
SUPFAMiSSF49452 SSF49452, 1 hit
SSF51445 SSF51445, 1 hit
SSF81296 SSF81296, 1 hit
PROSITEiView protein in PROSITE
PS51166 CBM20, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P05618-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKRFMKLTAV WTLWLSLTLG LLSPVHAAPD TSVSNKQNFS TDVIYQIFTD
60 70 80 90 100
RFSDGNPANN PTGAAFDGSC TNLRLYCGGD WQGIINKIND GYLTGMGITA
110 120 130 140 150
IWISQPVENI YSVINYSGVN NTAYHGYWAR DFKKTNPAYG TMQDFKNLID
160 170 180 190 200
TAHAHNIKVI IDFAPNHTSP ASSDDPSFAE NGRLYDNGNL LGGYTNDTQN
210 220 230 240 250
LFHHYGGTDF STIENGIYKN LYDLADLNHN NSSVDVYLKD AIKMWLDLGV
260 270 280 290 300
DGIRVDAVKH MPFGWQKSFM ATINNYKPVF TFGEWFLGVN EISPEYHQFA
310 320 330 340 350
NESGMSLLDF RFAQKARQVF RDNTDNMYGL KAMLEGSEVD YAQVNDQVTF
360 370 380 390 400
IDNHDMERFH TSNGDRRKLE QALAFTLTSR GVPAIYYGSE QYMSGGNDPD
410 420 430 440 450
NRARLPSFST TTTAYQVIQK LAPLRKSNPA IAYGSTHERW INNDVIIYER
460 470 480 490 500
KFGNNVAVVA INRNMNTPAS ITGLVTSLRR ASYNDVLGGI LNGNTLTVGA
510 520 530 540 550
GGAASNFTLA PGGTAVWQYT TDATTPIIGN VGPMMAKPGV TITIDGRGFG
560 570 580 590 600
SGKGTVYFGT TAVTGADIVA WEDTQIQVKI PAVPGGIYDI RVANAAGAAS
610 620 630 640 650
NIYDNFEVLT GDQVTVRFVI NNATTALGQN VFLTGNVSEL GNWDPNNAIG
660 670 680 690 700
PMYNQVVYQY PTWYYDVSVP AGQTIEFKFL KKQGSTVTWE GGANRTFTTP
710
TSGTATVNVN WQP
Length:713
Mass (Da):78,340
Last modified:November 1, 1988 - v1
Checksum:i524B259526B56C52
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17366 Genomic DNA Translation: AAA22308.1
PIRiA26678 ALBSG1

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17366 Genomic DNA Translation: AAA22308.1
PIRiA26678 ALBSG1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1D7FX-ray1.90A/B28-713[»]
1DEDX-ray2.00A/B28-713[»]
1I75X-ray2.00A/B28-713[»]
1PAMX-ray1.80A/B28-713[»]
1UKQX-ray2.00A/B28-713[»]
1UKSX-ray1.90A/B28-713[»]
1UKTX-ray2.20A/B28-713[»]
1V3JX-ray2.00A/B28-713[»]
1V3KX-ray2.00A/B28-713[»]
1V3LX-ray2.10A/B28-713[»]
1V3MX-ray2.00A/B28-713[»]
ProteinModelPortaliP05618
SMRiP05618
ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

DrugBankiDB02379 Beta-D-Glucose
DB03206 Duvoglustat

Protein family/group databases

CAZyiCBM20 Carbohydrate-Binding Module Family 20
GH13 Glycoside Hydrolase Family 13

Proteomic databases

PRIDEiP05618

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi2.4.1.19 691

Miscellaneous databases

EvolutionaryTraceiP05618

Family and domain databases

Gene3Di2.60.40.10, 2 hits
2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR006048 A-amylase/branching_C
IPR031319 A-amylase_C
IPR006046 Alpha_amylase
IPR013784 Carb-bd-like_fold
IPR002044 CBM_fam20
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
IPR013783 Ig-like_fold
IPR014756 Ig_E-set
IPR002909 IPT_dom
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF02806 Alpha-amylase_C, 1 hit
PF00686 CBM_20, 1 hit
PF01833 TIG, 1 hit
PRINTSiPR00110 ALPHAAMYLASE
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SM00632 Aamy_C, 1 hit
SM01065 CBM_2, 1 hit
SUPFAMiSSF49452 SSF49452, 1 hit
SSF51445 SSF51445, 1 hit
SSF81296 SSF81296, 1 hit
PROSITEiView protein in PROSITE
PS51166 CBM20, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCDGT_BACS0
AccessioniPrimary (citable) accession number: P05618
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: May 23, 2018
This is version 127 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again