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Protein

Integrin alpha-M

Gene

Itgam

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration (PubMed:8986723, PubMed:9382884, PubMed:9862668). In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (By similarity). May regulate phagocytosis-induced apoptosis in extravasated neutrophils (PubMed:8986723). May play a role in mast cell development (PubMed:9862668).By similarity3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi465 – 473Sequence analysis9
Calcium bindingi529 – 537Sequence analysis9
Calcium bindingi592 – 600Sequence analysis9

GO - Molecular functioni

  • complement component C3b binding Source: ARUK-UCL
  • heat shock protein binding Source: MGI
  • heparan sulfate proteoglycan binding Source: MGI
  • heparin binding Source: MGI
  • metal ion binding Source: UniProtKB-KW
  • opsonin binding Source: MGI
  • protein heterodimerization activity Source: MGI

GO - Biological processi

Keywordsi

Molecular functionIntegrin, Receptor
Biological processCell adhesion
LigandCalcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Integrin alpha-M
Alternative name(s):
CD11 antigen-like family member B
CR-3 alpha chain
Cell surface glycoprotein MAC-1 subunit alpha
Leukocyte adhesion receptor MO1
CD_antigen: CD11b
Gene namesi
Name:Itgam
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:96607 Itgam

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini17 – 1105ExtracellularSequence analysisAdd BLAST1089
Transmembranei1106 – 1129HelicalSequence analysisAdd BLAST24
Topological domaini1130 – 1153CytoplasmicSequence analysisAdd BLAST24

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice are born at the expected Mendelian rate (PubMed:8986723). The numbers of blood leukocytes are normal and neutrophils rolling on blood vessels is not affected (PubMed:8986723). Impaired LTB4-induced neutrophil adhesion to venules (PubMed:8986723). Numbers of mast cells in the peritoneal cavity, peritoneal wall and in the dorsal skin are reduced (PubMed:9862668). In an experimental model of thioglycolate-induced peritonitis, abnormal accumulation of recruited neutrophils due to impaired apoptosis (PubMed:8986723). In an experimental model of acute septic peritonitis, 5-fold increase in mortality rate, reduced neutrophil recruitment in the peritoneum, reduced histamine production and impaired bacterial clearence (PubMed:9862668). In experimental model of immune complex-mediated glomerulonephritis, reduced neutrophil accumulation without affecting their initial recruitment and reduced proteinuria (PubMed:9382884). Enhanced susceptibility to high fat diet-induced obesity characterized by a weight increase and higher levels of white and brown fat (PubMed:9207125).4 Publications

Chemistry databases

ChEMBLiCHEMBL3603

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 161 PublicationAdd BLAST16
ChainiPRO_000001629017 – 1153Integrin alpha-MAdd BLAST1137

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi58N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi66 ↔ 73By similarity
Glycosylationi86N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi105 ↔ 123By similarity
Glycosylationi391N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi654 ↔ 711By similarity
Glycosylationi696N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi734N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi770 ↔ 776By similarity
Glycosylationi772N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi801N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi881N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi907N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi941N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi980N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi994N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi999 ↔ 1023By similarity
Glycosylationi1022N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi1028 ↔ 1033By similarity
Glycosylationi1045N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1051N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1076N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP05555
PaxDbiP05555
PeptideAtlasiP05555
PRIDEiP05555

PTM databases

PhosphoSitePlusiP05555

Expressioni

Tissue specificityi

Predominantly expressed in monocytes and granulocytes (PubMed:3887182, PubMed:8986723). Expressed in a subset of peritoneal mast cells (PubMed:9862668).3 Publications

Inductioni

Induced by lipopolysaccharide (LPS) in mast cells.1 Publication

Gene expression databases

CleanExiMM_ITGAM

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta chain. ITGAM associates with ITGB2. Found in a complex with CD177 and ITGB2/CD18. Interacts with JAM3. Interacts with THBD.By similarity

GO - Molecular functioni

Protein-protein interaction databases

ComplexPortaliCPX-3129 Integrin alphaM-beta2 complex
IntActiP05555, 2 interactors
MINTiP05555
STRINGi10090.ENSMUSP00000101849

Structurei

3D structure databases

ProteinModelPortaliP05555
SMRiP05555
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati18 – 75FG-GAP 1PROSITE-ProRule annotationAdd BLAST58
Repeati76 – 135FG-GAP 2PROSITE-ProRule annotationAdd BLAST60
Domaini164 – 338VWFAPROSITE-ProRule annotationAdd BLAST175
Repeati339 – 390FG-GAP 3PROSITE-ProRule annotationAdd BLAST52
Repeati391 – 442FG-GAP 4PROSITE-ProRule annotationAdd BLAST52
Repeati443 – 503FG-GAP 5PROSITE-ProRule annotationAdd BLAST61
Repeati506 – 564FG-GAP 6PROSITE-ProRule annotationAdd BLAST59
Repeati569 – 629FG-GAP 7PROSITE-ProRule annotationAdd BLAST61

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi1132 – 1136GFFKR motif5

Domaini

The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.

Sequence similaritiesi

Belongs to the integrin alpha chain family.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IPBA Eukaryota
ENOG410ZFBE LUCA
HOGENOMiHOG000113114
HOVERGENiHBG100530
InParanoidiP05555
PhylomeDBiP05555

Family and domain databases

Gene3Di2.130.10.130, 1 hit
3.40.50.410, 1 hit
InterProiView protein in InterPro
IPR013517 FG-GAP
IPR013519 Int_alpha_beta-p
IPR000413 Integrin_alpha
IPR013649 Integrin_alpha-2
IPR018184 Integrin_alpha_C_CS
IPR028994 Integrin_alpha_N
IPR032695 Integrin_dom_sf
IPR002035 VWF_A
IPR036465 vWFA_dom_sf
PfamiView protein in Pfam
PF01839 FG-GAP, 1 hit
PF00357 Integrin_alpha, 1 hit
PF08441 Integrin_alpha2, 1 hit
PF00092 VWA, 1 hit
PRINTSiPR01185 INTEGRINA
SMARTiView protein in SMART
SM00191 Int_alpha, 5 hits
SM00327 VWA, 1 hit
SUPFAMiSSF53300 SSF53300, 1 hit
SSF69179 SSF69179, 3 hits
PROSITEiView protein in PROSITE
PS51470 FG_GAP, 7 hits
PS00242 INTEGRIN_ALPHA, 1 hit
PS50234 VWFA, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P05555-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MTLKALLVTA LALCHGFNLD TEHPMTFQEN AKGFGQNVVQ LGGTSVVVAA
60 70 80 90 100
PQEAKAVNQT GALYQCDYST SRCHPIPLQV PPEAVNMSLG LSLAVSTVPQ
110 120 130 140 150
QLLACGPTVH QNCKENTYVN GLCYLFGSNL LRPPQQFPEA LRECPQQESD
160 170 180 190 200
IVFLIDGSGS INNIDFQKMK EFVSTVMEQF KKSKTLFSLM QYSDEFRIHF
210 220 230 240 250
TFNDFKRNPS PRSHVSPIKQ LNGRTKTASG IRKVVRELFH KTNGARENAA
260 270 280 290 300
KILVVITDGE KFGDPLDYKD VIPEADRAGV IRYVIGVGNA FNKPQSRREL
310 320 330 340 350
DTIASKPAGE HVFQVDNFEA LNTIQNQLQE KIFAIEGTQT GSTSSFEHEM
360 370 380 390 400
SQEGFSASIT SNGPLLGSVG SFDWAGGAFL YTSKDKVTFI NTTRVDSDMN
410 420 430 440 450
DAYLGYASAV ILRNRVQSLV LGAPRYQHIG LVVMFRENFG TWEPHTSIKG
460 470 480 490 500
SQIGSYFGAS LCSVDMDADG NTNLILIGAP HYYEKTRGGQ VSVCPLPRGR
510 520 530 540 550
ARWQCEALLH GDQGHPWGRF GAALTVLGDV NGDKLTDVAI GAPGEQENQG
560 570 580 590 600
AVYIFYGASI ASLSASHSHR IIGAHFSPGL QYFGQSLSGG KDLTMDGLMD
610 620 630 640 650
LAVGAQGHLL LLRAQPVLRL EATMEFSPKK VARSVFACQE QVLKNKDAGE
660 670 680 690 700
VRVCLRVRKN TKDRLREGDI QSTVTYDLAL DPVRSRIRAF FDETKNNTRR
710 720 730 740 750
RTQVFGLMQK CETLKLILPD CVDDSVSPII LRLNYTLVGE PLRSFGNLRP
760 770 780 790 800
VLAMDAQRFF TAMFPFEKNC GNDSICQDDL SITMSAMGLD TLVVGGPQDF
810 820 830 840 850
NMSVTLRNDG EDSYGTQVTV YYPSGLSYRK DSASQNPLTK KPWFVKPAES
860 870 880 890 900
SSSSEGHGAL KSTTWNINHP IFPANSEVTF NVTFDVDSHA SFGNKLLLKA
910 920 930 940 950
IVASENNMSR THKTKFQLEL PVKYAIYMIV TSDESSIRYL NFTASEMTSK
960 970 980 990 1000
VIQHQYQFNN LGQRSLPVSV VFWIPVQINN VTVWDHPQVI FSQNLSSACH
1010 1020 1030 1040 1050
TEQKSPPHSN FRDQLERTPV LNCSVAVCKR IQCDLPSFNT QEIFNVTLKG
1060 1070 1080 1090 1100
NLSFDWYIKT SHGHLLLVSS TEILFNDSAF ALLPGQESYV RSKTETKVEP
1110 1120 1130 1140 1150
YEVHNPVPLI VGSSIGGLVL LALITAGLYK LGFFKRQYKD MMNEAAPQDA

PPQ
Length:1,153
Mass (Da):127,481
Last modified:February 1, 1991 - v2
Checksum:i178DB988AECB0343
GO
Isoform 2 (identifier: P05555-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     453-569: Missing.

Note: No experimental confirmation available.
Show »
Length:1,036
Mass (Da):115,196
Checksum:i489843932CDBA7D2
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
G5E8F1G5E8F1_MOUSE
Integrin alpha-M
Itgam
1,154Annotation score:
E9Q604E9Q604_MOUSE
Integrin alpha M, isoform CRA_a
Itgam mCG_133489
1,153Annotation score:
A0A0R4J1B4A0A0R4J1B4_MOUSE
Integrin alpha-M
Itgam
1,036Annotation score:
E9Q5K8E9Q5K8_MOUSE
Integrin alpha-M
Itgam
1,167Annotation score:
D6RJ73D6RJ73_MOUSE
Integrin alpha-M
Itgam
94Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti37N → S in BAC30350 (PubMed:16141072).Curated1
Sequence conflicti683V → G in BAC30350 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_010473453 – 569Missing in isoform 2. 1 PublicationAdd BLAST117

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07640 mRNA Translation: CAA30479.1
AK039444 mRNA Translation: BAC30350.1
M14293 Genomic DNA Translation: AAA39484.1
CCDSiCCDS21889.1 [P05555-1]
PIRiS00551
UniGeneiMm.262106

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07640 mRNA Translation: CAA30479.1
AK039444 mRNA Translation: BAC30350.1
M14293 Genomic DNA Translation: AAA39484.1
CCDSiCCDS21889.1 [P05555-1]
PIRiS00551
UniGeneiMm.262106

3D structure databases

ProteinModelPortaliP05555
SMRiP05555
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

ComplexPortaliCPX-3129 Integrin alphaM-beta2 complex
IntActiP05555, 2 interactors
MINTiP05555
STRINGi10090.ENSMUSP00000101849

Chemistry databases

ChEMBLiCHEMBL3603

PTM databases

PhosphoSitePlusiP05555

Proteomic databases

MaxQBiP05555
PaxDbiP05555
PeptideAtlasiP05555
PRIDEiP05555

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:96607 Itgam

Phylogenomic databases

eggNOGiENOG410IPBA Eukaryota
ENOG410ZFBE LUCA
HOGENOMiHOG000113114
HOVERGENiHBG100530
InParanoidiP05555
PhylomeDBiP05555

Miscellaneous databases

ChiTaRSiItgam mouse
PROiPR:P05555
SOURCEiSearch...

Gene expression databases

CleanExiMM_ITGAM

Family and domain databases

Gene3Di2.130.10.130, 1 hit
3.40.50.410, 1 hit
InterProiView protein in InterPro
IPR013517 FG-GAP
IPR013519 Int_alpha_beta-p
IPR000413 Integrin_alpha
IPR013649 Integrin_alpha-2
IPR018184 Integrin_alpha_C_CS
IPR028994 Integrin_alpha_N
IPR032695 Integrin_dom_sf
IPR002035 VWF_A
IPR036465 vWFA_dom_sf
PfamiView protein in Pfam
PF01839 FG-GAP, 1 hit
PF00357 Integrin_alpha, 1 hit
PF08441 Integrin_alpha2, 1 hit
PF00092 VWA, 1 hit
PRINTSiPR01185 INTEGRINA
SMARTiView protein in SMART
SM00191 Int_alpha, 5 hits
SM00327 VWA, 1 hit
SUPFAMiSSF53300 SSF53300, 1 hit
SSF69179 SSF69179, 3 hits
PROSITEiView protein in PROSITE
PS51470 FG_GAP, 7 hits
PS00242 INTEGRIN_ALPHA, 1 hit
PS50234 VWFA, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiITAM_MOUSE
AccessioniPrimary (citable) accession number: P05555
Secondary accession number(s): Q8CA73
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: February 1, 1991
Last modified: November 7, 2018
This is version 167 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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