UniProtKB - P05458 (PTRA_ECOLI)
Protein
Protease 3
Gene
ptrA
Organism
Escherichia coli (strain K12)
Status
Functioni
Endopeptidase that degrades small peptides of less than 7 kDa, such as glucagon and insulin.
Catalytic activityi
- Preferential cleavage of 16-Tyr-|-Leu-17 and 25-Phe-|-Tyr-26 bonds of oxidized insulin B chain. Also acts on other substrates of Mw less than 7 kDa such as insulin and glucagon.PROSITE-ProRule annotation EC:3.4.24.55
Cofactori
Zn2+Note: Binds 1 zinc ion per subunit.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 88 | ZincPROSITE-ProRule annotation1 Publication | 1 | |
Active sitei | 91 | Proton acceptorPROSITE-ProRule annotation2 Publications | 1 | |
Metal bindingi | 92 | ZincPROSITE-ProRule annotation1 Publication | 1 | |
Metal bindingi | 169 | Zinc | 1 |
GO - Molecular functioni
- metalloendopeptidase activity Source: EcoliWiki
- zinc ion binding Source: EcoliWiki
GO - Biological processi
- proteolysis Source: EcoliWiki
Keywordsi
Molecular function | Hydrolase, Metalloprotease, Protease |
Ligand | Magnesium, Metal-binding, Zinc |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10786-MONOMER MetaCyc:EG10786-MONOMER |
Protein family/group databases
MEROPSi | M16.001 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:ptrA Synonyms:ptr Ordered Locus Names:b2821, JW2789 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
GO - Cellular componenti
- cytoplasm Source: EcoliWiki
- outer membrane-bounded periplasmic space Source: EcoCyc
Keywords - Cellular componenti
PeriplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 88 | H → R: Loss of activity and of Zn-binding. | 1 | |
Mutagenesisi | 91 | E → Q: Loss of activity. | 1 | |
Mutagenesisi | 92 | H → R: Loss of activity and of Zn-binding. | 1 | |
Mutagenesisi | 162 | E → Q: 20% loss of activity. | 1 | |
Mutagenesisi | 169 | E → Q: Loss of activity and of Zn-binding. | 1 | |
Mutagenesisi | 204 | E → Q: No loss of activity. | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 23 | Add BLAST | 23 | |
ChainiPRO_0000026758 | 24 – 962 | Protease 3Add BLAST | 939 |
Proteomic databases
jPOSTi | P05458 |
PaxDbi | P05458 |
PRIDEi | P05458 |
Interactioni
Subunit structurei
Monomer.
Protein-protein interaction databases
BioGRIDi | 4262308, 35 interactors |
IntActi | P05458, 12 interactors |
STRINGi | 511145.b2821 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P05458 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P05458 |
Family & Domainsi
Sequence similaritiesi
Belongs to the peptidase M16 family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG1025, Bacteria |
HOGENOMi | CLU_004639_1_3_6 |
InParanoidi | P05458 |
PhylomeDBi | P05458 |
Family and domain databases
InterProi | View protein in InterPro IPR011249, Metalloenz_LuxS/M16 IPR011765, Pept_M16_N IPR001431, Pept_M16_Zn_BS IPR007863, Peptidase_M16_C IPR032632, Peptidase_M16_M |
Pfami | View protein in Pfam PF00675, Peptidase_M16, 1 hit PF05193, Peptidase_M16_C, 2 hits PF16187, Peptidase_M16_M, 1 hit |
SUPFAMi | SSF63411, SSF63411, 4 hits |
PROSITEi | View protein in PROSITE PS00143, INSULINASE, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P05458-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPRSTWFKAL LLLVALWAPL SQAETGWQPI QETIRKSDKD NRQYQAIRLD
60 70 80 90 100
NGMVVLLVSD PQAVKSLSAL VVPVGSLEDP EAYQGLAHYL EHMSLMGSKK
110 120 130 140 150
YPQADSLAEY LKMHGGSHNA STAPYRTAFY LEVENDALPG AVDRLADAIA
160 170 180 190 200
EPLLDKKYAE RERNAVNAEL TMARTRDGMR MAQVSAETIN PAHPGSKFSG
210 220 230 240 250
GNLETLSDKP GNPVQQALKD FHEKYYSANL MKAVIYSNKP LPELAKMAAD
260 270 280 290 300
TFGRVPNKES KKPEITVPVV TDAQKGIIIH YVPALPRKVL RVEFRIDNNS
310 320 330 340 350
AKFRSKTDEL ITYLIGNRSP GTLSDWLQKQ GLVEGISANS DPIVNGNSGV
360 370 380 390 400
LAISASLTDK GLANRDQVVA AIFSYLNLLR EKGIDKQYFD ELANVLDIDF
410 420 430 440 450
RYPSITRDMD YVEWLADTMI RVPVEHTLDA VNIADRYDAK AVKERLAMMT
460 470 480 490 500
PQNARIWYIS PKEPHNKTAY FVDAPYQVDK ISAQTFADWQ KKAADIALSL
510 520 530 540 550
PELNPYIPDD FSLIKSEKKY DHPELIVDES NLRVVYAPSR YFASEPKADV
560 570 580 590 600
SLILRNPKAM DSARNQVMFA LNDYLAGLAL DQLSNQASVG GISFSTNANN
610 620 630 640 650
GLMVNANGYT QRLPQLFQAL LEGYFSYTAT EDQLEQAKSW YNQMMDSAEK
660 670 680 690 700
GKAFEQAIMP AQMLSQVPYF SRDERRKILP SITLKEVLAY RDALKSGARP
710 720 730 740 750
EFMVIGNMTE AQATTLARDV QKQLGADGSE WCRNKDVVVD KKQSVIFEKA
760 770 780 790 800
GNSTDSALAA VFVPTGYDEY TSSAYSSLLG QIVQPWFYNQ LRTEEQLGYA
810 820 830 840 850
VFAFPMSVGR QWGMGFLLQS NDKQPSFLWE RYKAFFPTAE AKLRAMKPDE
860 870 880 890 900
FAQIQQAVIT QMLQAPQTLG EEASKLSKDF DRGNMRFDSR DKIVAQIKLL
910 920 930 940 950
TPQKLADFFH QAVVEPQGMA ILSQISGSQN GKAEYVHPEG WKVWENVSAL
960
QQTMPLMSEK NE
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 277 – 284 | IIIHYVPA → HYHSLRPW in AAA24436 (PubMed:3308636).Curated | 8 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X04581 Genomic DNA Translation: CAA28249.1 U29581 Genomic DNA Translation: AAB40468.1 U00096 Genomic DNA Translation: AAC75860.1 AP009048 Genomic DNA Translation: BAE76890.1 X06227 Genomic DNA Translation: CAA29576.1 M17095 Genomic DNA Translation: AAA24436.1 |
PIRi | F65064, SNECPI |
RefSeqi | NP_417298.1, NC_000913.3 WP_001138201.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75860; AAC75860; b2821 BAE76890; BAE76890; BAE76890 |
GeneIDi | 947284 |
KEGGi | ecj:JW2789 eco:b2821 |
PATRICi | fig|1411691.4.peg.3915 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X04581 Genomic DNA Translation: CAA28249.1 U29581 Genomic DNA Translation: AAB40468.1 U00096 Genomic DNA Translation: AAC75860.1 AP009048 Genomic DNA Translation: BAE76890.1 X06227 Genomic DNA Translation: CAA29576.1 M17095 Genomic DNA Translation: AAA24436.1 |
PIRi | F65064, SNECPI |
RefSeqi | NP_417298.1, NC_000913.3 WP_001138201.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1Q2L | X-ray | 2.20 | A | 24-962 | [»] | |
SMRi | P05458 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262308, 35 interactors |
IntActi | P05458, 12 interactors |
STRINGi | 511145.b2821 |
Protein family/group databases
MEROPSi | M16.001 |
Proteomic databases
jPOSTi | P05458 |
PaxDbi | P05458 |
PRIDEi | P05458 |
Genome annotation databases
EnsemblBacteriai | AAC75860; AAC75860; b2821 BAE76890; BAE76890; BAE76890 |
GeneIDi | 947284 |
KEGGi | ecj:JW2789 eco:b2821 |
PATRICi | fig|1411691.4.peg.3915 |
Organism-specific databases
EchoBASEi | EB0779 |
Phylogenomic databases
eggNOGi | COG1025, Bacteria |
HOGENOMi | CLU_004639_1_3_6 |
InParanoidi | P05458 |
PhylomeDBi | P05458 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10786-MONOMER MetaCyc:EG10786-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P05458 |
PROi | PR:P05458 |
Family and domain databases
InterProi | View protein in InterPro IPR011249, Metalloenz_LuxS/M16 IPR011765, Pept_M16_N IPR001431, Pept_M16_Zn_BS IPR007863, Peptidase_M16_C IPR032632, Peptidase_M16_M |
Pfami | View protein in Pfam PF00675, Peptidase_M16, 1 hit PF05193, Peptidase_M16_C, 2 hits PF16187, Peptidase_M16_M, 1 hit |
SUPFAMi | SSF63411, SSF63411, 4 hits |
PROSITEi | View protein in PROSITE PS00143, INSULINASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PTRA_ECOLI | |
Accessioni | P05458Primary (citable) accession number: P05458 Secondary accession number(s): P78106, Q2MA16 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1988 |
Last sequence update: | November 1, 1988 | |
Last modified: | December 2, 2020 | |
This is version 179 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families