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Protein

DNA-directed RNA polymerase III subunit RPC4

Gene

POLR3D

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway (By similarity).By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processAntiviral defense, Immunity, Innate immunity, Transcription

Enzyme and pathway databases

ReactomeiR-HSA-1834949 Cytosolic sensors of pathogen-associated DNA
R-HSA-73780 RNA Polymerase III Chain Elongation
R-HSA-73980 RNA Polymerase III Transcription Termination
R-HSA-749476 RNA Polymerase III Abortive And Retractive Initiation
R-HSA-76061 RNA Polymerase III Transcription Initiation From Type 1 Promoter
R-HSA-76066 RNA Polymerase III Transcription Initiation From Type 2 Promoter
R-HSA-76071 RNA Polymerase III Transcription Initiation From Type 3 Promoter

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase III subunit RPC4
Short name:
RNA polymerase III subunit C4
Alternative name(s):
DNA-directed RNA polymerase III subunit D
Protein BN51
RNA polymerase III 47 kDa subunit
RPC53 homolog
Gene namesi
Name:POLR3D
Synonyms:BN51, BN51T
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

EuPathDBiHostDB:ENSG00000168495.12
HGNCiHGNC:1080 POLR3D
MIMi187280 gene
neXtProtiNX_P05423

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000168495
PharmGKBiPA25390

Polymorphism and mutation databases

BioMutaiPOLR3D
DMDMi29429159

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000739672 – 398DNA-directed RNA polymerase III subunit RPC4Add BLAST397

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei42PhosphoserineCombined sources1
Cross-linki68Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki78Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei95Omega-N-methylarginineCombined sources1
Modified residuei97Omega-N-methylarginineCombined sources1
Modified residuei99Omega-N-methylarginineCombined sources1
Cross-linki141Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki152Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki160Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki190Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki199Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki206Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki220Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki285Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki302Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki310Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki396Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP05423
MaxQBiP05423
PaxDbiP05423
PeptideAtlasiP05423
PRIDEiP05423
ProteomicsDBi51838

PTM databases

iPTMnetiP05423
PhosphoSitePlusiP05423

Expressioni

Gene expression databases

BgeeiENSG00000168495 Expressed in 173 organ(s), highest expression level in gastrocnemius
CleanExiHS_POLR3D
ExpressionAtlasiP05423 baseline and differential
GenevisibleiP05423 HS

Organism-specific databases

HPAiHPA061331
HPA067875
HPA068461
HPA069089

Interactioni

Subunit structurei

Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits (By similarity). Interacts with POLR3E/RPC5.By similarity1 Publication

Protein-protein interaction databases

BioGridi107129, 41 interactors
DIPiDIP-56155N
IntActiP05423, 11 interactors
MINTiP05423
STRINGi9606.ENSP00000303088

Structurei

3D structure databases

ProteinModelPortaliP05423
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3122 Eukaryota
ENOG410XPDH LUCA
GeneTreeiENSGT00390000013948
HOGENOMiHOG000008080
HOVERGENiHBG039777
InParanoidiP05423
KOiK03026
OMAiWDKTVDM
OrthoDBiEOG091G13BB
PhylomeDBiP05423

Family and domain databases

InterProiView protein in InterPro
IPR007811 RPC4
PANTHERiPTHR13408 PTHR13408, 1 hit
PfamiView protein in Pfam
PF05132 RNA_pol_Rpc4, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

P05423-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSEGNAAGEP STPGGPRPLL TGARGLIGRR PAPPLTPGRL PSIRSRDLTL
60 70 80 90 100
GGVKKKTFTP NIISRKIKEE PKEEVTVKKE KRERDRDRQR EGHGRGRGRP
110 120 130 140 150
EVIQSHSIFE QGPAEMMKKK GNWDKTVDVS DMGPSHIINI KKEKRETDEE
160 170 180 190 200
TKQILRMLEK DDFLDDPGLR NDTRNMPVQL PLAHSGWLFK EENDEPDVKP
210 220 230 240 250
WLAGPKEEDM EVDIPAVKVK EEPRDEEEEA KMKAPPKAAR KTPGLPKDVS
260 270 280 290 300
VAELLRELSL TKEEELLFLQ LPDTLPGQPP TQDIKPIKTE VQGEDGQVVL
310 320 330 340 350
IKQEKDREAK LAENACTLAD LTEGQVGKLL IRKSGRVQLL LGKVTLDVTM
360 370 380 390
GTACSFLQEL VSVGLGDSRT GEMTVLGHVK HKLVCSPDFE SLLDHKHR
Length:398
Mass (Da):44,396
Last modified:March 28, 2003 - v2
Checksum:iCD8AFF3257B78410
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E5RHT4E5RHT4_HUMAN
DNA-directed RNA polymerase III sub...
POLR3D
207Annotation score:
E7EQ68E7EQ68_HUMAN
DNA-directed RNA polymerase III sub...
POLR3D
123Annotation score:

Sequence cautioni

The sequence BC000516 differs from that shown. Reason: Frameshift at position 175.Curated
The sequence BC003039 differs from that shown. Reason: Frameshift at position 175.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti9 – 18EPSTPGGPRP → RPARQGPDL in AAA51838 (PubMed:3683386).Curated10
Sequence conflicti9 – 12EPST → RPAR in AAA72377 (Ref. 6) Curated4
Sequence conflicti26 – 40LIGRR…TPGRL → SSGGGGLPSPPAV (PubMed:3683386).CuratedAdd BLAST15
Sequence conflicti98G → R in AAA51838 (PubMed:3683386).Curated1
Sequence conflicti233K → R in AAM18216 (PubMed:12391170).Curated1
Sequence conflicti286P → L in AAM18216 (PubMed:12391170).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17754 mRNA Translation: AAA51838.1
AY092086 mRNA Translation: AAM18216.1
CR536509 mRNA Translation: CAG38747.1
CR541803 mRNA Translation: CAG46602.1
BC000516 mRNA No translation available.
BC003039 mRNA No translation available.
BC002603 mRNA Translation: AAH02603.1
BC004484 mRNA Translation: AAH04484.1
AF346574 mRNA Translation: AAK15371.1
L15301 Genomic DNA Translation: AAA72377.1
CCDSiCCDS34858.1
PIRiA43700
RefSeqiNP_001713.2, NM_001722.2
UniGeneiHs.148342
Hs.262907

Genome annotation databases

EnsembliENST00000306433; ENSP00000303088; ENSG00000168495
ENST00000397802; ENSP00000380904; ENSG00000168495
GeneIDi661
KEGGihsa:661
UCSCiuc003xbl.4 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17754 mRNA Translation: AAA51838.1
AY092086 mRNA Translation: AAM18216.1
CR536509 mRNA Translation: CAG38747.1
CR541803 mRNA Translation: CAG46602.1
BC000516 mRNA No translation available.
BC003039 mRNA No translation available.
BC002603 mRNA Translation: AAH02603.1
BC004484 mRNA Translation: AAH04484.1
AF346574 mRNA Translation: AAK15371.1
L15301 Genomic DNA Translation: AAA72377.1
CCDSiCCDS34858.1
PIRiA43700
RefSeqiNP_001713.2, NM_001722.2
UniGeneiHs.148342
Hs.262907

3D structure databases

ProteinModelPortaliP05423
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107129, 41 interactors
DIPiDIP-56155N
IntActiP05423, 11 interactors
MINTiP05423
STRINGi9606.ENSP00000303088

PTM databases

iPTMnetiP05423
PhosphoSitePlusiP05423

Polymorphism and mutation databases

BioMutaiPOLR3D
DMDMi29429159

Proteomic databases

EPDiP05423
MaxQBiP05423
PaxDbiP05423
PeptideAtlasiP05423
PRIDEiP05423
ProteomicsDBi51838

Protocols and materials databases

DNASUi661
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000306433; ENSP00000303088; ENSG00000168495
ENST00000397802; ENSP00000380904; ENSG00000168495
GeneIDi661
KEGGihsa:661
UCSCiuc003xbl.4 human

Organism-specific databases

CTDi661
EuPathDBiHostDB:ENSG00000168495.12
GeneCardsiPOLR3D
HGNCiHGNC:1080 POLR3D
HPAiHPA061331
HPA067875
HPA068461
HPA069089
MIMi187280 gene
neXtProtiNX_P05423
OpenTargetsiENSG00000168495
PharmGKBiPA25390
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3122 Eukaryota
ENOG410XPDH LUCA
GeneTreeiENSGT00390000013948
HOGENOMiHOG000008080
HOVERGENiHBG039777
InParanoidiP05423
KOiK03026
OMAiWDKTVDM
OrthoDBiEOG091G13BB
PhylomeDBiP05423

Enzyme and pathway databases

ReactomeiR-HSA-1834949 Cytosolic sensors of pathogen-associated DNA
R-HSA-73780 RNA Polymerase III Chain Elongation
R-HSA-73980 RNA Polymerase III Transcription Termination
R-HSA-749476 RNA Polymerase III Abortive And Retractive Initiation
R-HSA-76061 RNA Polymerase III Transcription Initiation From Type 1 Promoter
R-HSA-76066 RNA Polymerase III Transcription Initiation From Type 2 Promoter
R-HSA-76071 RNA Polymerase III Transcription Initiation From Type 3 Promoter

Miscellaneous databases

GeneWikiiPOLR3D
GenomeRNAii661
PROiPR:P05423
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000168495 Expressed in 173 organ(s), highest expression level in gastrocnemius
CleanExiHS_POLR3D
ExpressionAtlasiP05423 baseline and differential
GenevisibleiP05423 HS

Family and domain databases

InterProiView protein in InterPro
IPR007811 RPC4
PANTHERiPTHR13408 PTHR13408, 1 hit
PfamiView protein in Pfam
PF05132 RNA_pol_Rpc4, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRPC4_HUMAN
AccessioniPrimary (citable) accession number: P05423
Secondary accession number(s): Q6FI28
, Q9BPV7, Q9BPZ1, Q9BXB3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: March 28, 2003
Last modified: October 10, 2018
This is version 172 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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