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Entry version 172 (29 Sep 2021)
Sequence version 1 (01 Nov 1988)
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Protein

Phosphatidyl-N-methylethanolamine N-methyltransferase

Gene

OPI3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the second two steps of the methylation pathway of phosphatidylcholine biosynthesis, the SAM-dependent methylation of phosphatidylmonomethylethanolamine (PMME) to phosphatidyldimethylethanolamine (PDME) and of PDME to phosphatidylcholine (PC). Can also catalyze the first methylation reaction of PE to PMME in the absence of PE methyltransferase CHO2.

UniRule annotation8 Publications

Miscellaneous

Present with 5890 molecules/cell in log phase SD medium.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

  1. KM=180 µM for S-adenosyl-L-methionine (in presence of phosphatidylethanolamine (PE) as substrate)1 Publication
  2. KM=190 µM for S-adenosyl-L-methionine (in presence of phosphatidyl-N-methylethanolamine (PMME) as substrate)1 Publication
  3. KM=240 µM for S-adenosyl-L-methionine (in presence of phosphatidyl-N-dimethylethanolamine (PDME) as substrate)1 Publication
  4. KM=270 µM for phosphatidyl-N-methylethanolamine (PMME)1 Publication
  5. KM=110 µM for phosphatidyl-N-dimethylethanolamine (PDME)1 Publication
  6. KM=54 µM for S-adenosyl-L-methionine (in presence of phosphatidyl-N-methylethanolamine (PMME) as substrate)1 Publication
  7. KM=59 µM for S-adenosyl-L-methionine (in presence of phosphatidyl-N-dimethylethanolamine (PDME) as substrate)1 Publication
  8. KM=380 µM for phosphatidyl-N-methylethanolamine (PMME)1 Publication
  9. KM=180 µM for phosphatidyl-N-dimethylethanolamine (PDME)1 Publication

pH dependencei

Optimum pH is 8.1.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: phosphatidylcholine biosynthesis

This protein is involved in the pathway phosphatidylcholine biosynthesis, which is part of Phospholipid metabolism.UniRule annotation1 Publication
View all proteins of this organism that are known to be involved in the pathway phosphatidylcholine biosynthesis and in Phospholipid metabolism.

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processLipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
MetaCyc:YJR073C-MONOMER

Reactome - a knowledgebase of biological pathways and processes

More...
Reactomei
R-SCE-1483191, Synthesis of PC

UniPathway: a resource for the exploration and annotation of metabolic pathways

More...
UniPathwayi
UPA00753

Chemistry databases

SwissLipids knowledge resource for lipid biology

More...
SwissLipidsi
SLP:000000084

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Phosphatidyl-N-methylethanolamine N-methyltransferaseUniRule annotationCurated (EC:2.1.1.173 Publications, EC:2.1.1.71UniRule annotation4 Publications)
Alternative name(s):
Overproducer of inositol protein 31 Publication
Phospholipid methyltransferase2 PublicationsUniRule annotation
Short name:
PLMT2 PublicationsUniRule annotation
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:OPI31 Publication
Synonyms:PEM21 Publication
Ordered Locus Names:YJR073CImported
ORF Names:J1824
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri559292 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002311 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome X

Organism-specific databases

Saccharomyces Genome Database

More...
SGDi
S000003834, OPI3

Eukaryotic Pathogen, Vector and Host Database Resources

More...
VEuPathDBi
FungiDB:YJR073C

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular_location_section">'Subcellular location'</a> section describes the subcellular compartment where each non-membrane region of a membrane-spanning protein is found.<p><a href='/help/topo_dom' target='_top'>More...</a></p>Topological domaini1 – 20LumenalUniRule annotationAdd BLAST20
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular_location_section">'Subcellular location'</a> section describes the extent of a region that is buried within a membrane, but does not cross it.<p><a href='/help/intramem' target='_top'>More...</a></p>Intramembranei21 – 41HelicalUniRule annotationAdd BLAST21
Topological domaini42 – 53LumenalUniRule annotationAdd BLAST12
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular_location_section">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei54 – 74HelicalUniRule annotationAdd BLAST21
Topological domaini75 – 101CytoplasmicUniRule annotationAdd BLAST27
Transmembranei102 – 122HelicalUniRule annotationAdd BLAST21
Topological domaini123 – 165LumenalUniRule annotationAdd BLAST43
Transmembranei166 – 186HelicalUniRule annotationAdd BLAST21
Topological domaini187 – 206CytoplasmicUniRule annotationAdd BLAST20

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Mitochondrion

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001939241 – 206Phosphatidyl-N-methylethanolamine N-methyltransferaseAdd BLAST206

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P05375

PRoteomics IDEntifications database

More...
PRIDEi
P05375

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

Expression is repressed by inositol and choline. The 5' flanking region contains two copies of the CATRTGAA motif and a 5'-AAACCCACACATG-3' GRFI site, which are involved in the regulation of expression. OPI1 and SIN3 play the role of repressors for OPI3 expression whereas UME6 is an activator of OPI3 expression.4 Publications

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...
BioGRIDi
33829, 614 interactors

Database of interacting proteins

More...
DIPi
DIP-2888N

Protein interaction database and analysis system

More...
IntActi
P05375, 3 interactors

STRING: functional protein association networks

More...
STRINGi
4932.YJR073C

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...
RNActi
P05375, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni106 – 108S-adenosyl-L-methionine bindingUniRule annotation3
Regioni188 – 189S-adenosyl-L-methionine bindingUniRule annotation2

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the class VI-like SAM-binding methyltransferase superfamily. PEMT/PEM2 methyltransferase family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG4142, Eukaryota

Ensembl GeneTree

More...
GeneTreei
ENSGT00390000007041

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_086119_0_0_1

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P05375

Identification of Orthologs from Complete Genome Data

More...
OMAi
FNPTFWN

Family and domain databases

HAMAP database of protein families

More...
HAMAPi
MF_03216, PLMT, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR024960, PEMT/MFAP
IPR007318, Phopholipid_MeTrfase

The PANTHER Classification System

More...
PANTHERi
PTHR15458, PTHR15458, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF04191, PEMT, 1 hit

PIRSF; a whole-protein classification database

More...
PIRSFi
PIRSF005444, PEMT, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS51599, SAM_PEMT_PEM2, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P05375-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKESVQEIIQ QLIHSVDLQS SKFQLAIVCT MFNPIFWNIV ARMEYHKHSL
60 70 80 90 100
TKMCGGARKG CYMLAATIFS LGIVRDMVYE SALREQPTCS LITGENWTKL
110 120 130 140 150
GVALFGLGQV LVLSSMYKLG ITGTYLGDYF GILMDERVTG FPFNVSNNPM
160 170 180 190 200
YQGSTLSFLG IALYKGKPAG LVVSAVVYFM YKIALRWEEP FTAMIYANRD

KAKKNM
Length:206
Mass (Da):23,150
Last modified:November 1, 1988 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iBC0CDCC1C7B2AD36
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
M16988 Genomic DNA Translation: AAA34851.1
Z49573 Genomic DNA Translation: CAA89601.1
L47993 Genomic DNA Translation: AAB39298.1
AY557899 Genomic DNA Translation: AAS56225.1
BK006943 Genomic DNA Translation: DAA08859.1

Protein sequence database of the Protein Information Resource

More...
PIRi
B28443

NCBI Reference Sequences

More...
RefSeqi
NP_012607.1, NM_001181731.1

Genome annotation databases

Ensembl fungal genome annotation project

More...
EnsemblFungii
YJR073C_mRNA; YJR073C; YJR073C

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
853536

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
sce:YJR073C

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16988 Genomic DNA Translation: AAA34851.1
Z49573 Genomic DNA Translation: CAA89601.1
L47993 Genomic DNA Translation: AAB39298.1
AY557899 Genomic DNA Translation: AAS56225.1
BK006943 Genomic DNA Translation: DAA08859.1
PIRiB28443
RefSeqiNP_012607.1, NM_001181731.1

3D structure databases

Database of comparative protein structure models

More...
ModBasei
Search...

SWISS-MODEL Interactive Workspace

More...
SWISS-MODEL-Workspacei
Submit a new modelling project...

Protein-protein interaction databases

BioGRIDi33829, 614 interactors
DIPiDIP-2888N
IntActiP05375, 3 interactors
STRINGi4932.YJR073C

Chemistry databases

SwissLipidsiSLP:000000084

Proteomic databases

PaxDbiP05375
PRIDEiP05375

Protocols and materials databases

The DNASU plasmid repository

More...
DNASUi
853536

Genome annotation databases

EnsemblFungiiYJR073C_mRNA; YJR073C; YJR073C
GeneIDi853536
KEGGisce:YJR073C

Organism-specific databases

SGDiS000003834, OPI3
VEuPathDBiFungiDB:YJR073C

Phylogenomic databases

eggNOGiKOG4142, Eukaryota
GeneTreeiENSGT00390000007041
HOGENOMiCLU_086119_0_0_1
InParanoidiP05375
OMAiFNPTFWN

Enzyme and pathway databases

UniPathwayiUPA00753
BioCyciMetaCyc:YJR073C-MONOMER
ReactomeiR-SCE-1483191, Synthesis of PC

Miscellaneous databases

Protein Ontology

More...
PROi
PR:P05375
RNActiP05375, protein

Family and domain databases

HAMAPiMF_03216, PLMT, 1 hit
InterProiView protein in InterPro
IPR024960, PEMT/MFAP
IPR007318, Phopholipid_MeTrfase
PANTHERiPTHR15458, PTHR15458, 1 hit
PfamiView protein in Pfam
PF04191, PEMT, 1 hit
PIRSFiPIRSF005444, PEMT, 1 hit
PROSITEiView protein in PROSITE
PS51599, SAM_PEMT_PEM2, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiPLMT_YEAST
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P05375
Secondary accession number(s): D6VWP3
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: September 29, 2021
This is version 172 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families
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