UniProtKB - P05202 (AATM_MOUSE)
Protein
Aspartate aminotransferase, mitochondrial
Gene
Got2
Organism
Mus musculus (Mouse)
Status
Functioni
Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). As a member of the malate-aspartate shuttle, it has a key role in the intracellular NAD(H) redox balance. Is important for metabolite exchange between mitochondria and cytosol, and for amino acid metabolism. Facilitates cellular uptake of long-chain free fatty acids.2 Publications
Miscellaneous
In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.
Catalytic activityi
Cofactori
pyridoxal 5'-phosphate1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 65 | Substrate; via amide nitrogenBy similarity | 1 | |
Binding sitei | 162 | SubstrateBy similarity | 1 | |
Binding sitei | 215 | Substrate | 1 | |
Binding sitei | 407 | Substrate | 1 |
GO - Molecular functioni
- amino acid binding Source: MGI
- carboxylic acid binding Source: MGI
- enzyme binding Source: MGI
- identical protein binding Source: MGI
- kynurenine-oxoglutarate transaminase activity Source: UniProtKB-EC
- L-aspartate:2-oxoglutarate aminotransferase activity Source: MGI
- phospholipid binding Source: MGI
- pyridoxal phosphate binding Source: MGI
GO - Biological processi
- 2-oxoglutarate metabolic process Source: UniProtKB
- aspartate biosynthetic process Source: MGI
- aspartate catabolic process Source: MGI
- aspartate metabolic process Source: UniProtKB
- cellular amino acid metabolic process Source: MGI
- dicarboxylic acid metabolic process Source: MGI
- fatty acid transport Source: Ensembl
- female pregnancy Source: Ensembl
- glutamate catabolic process to 2-oxoglutarate Source: MGI
- glutamate catabolic process to aspartate Source: MGI
- glutamate metabolic process Source: UniProtKB
- lactation Source: Ensembl
- oxaloacetate metabolic process Source: MGI
- response to ethanol Source: MGI
- response to insulin Source: Ensembl
- response to morphine Source: Ensembl
- response to muscle activity Source: Ensembl
Keywordsi
Molecular function | Aminotransferase, Transferase |
Biological process | Lipid transport, Transport |
Ligand | Pyridoxal phosphate |
Enzyme and pathway databases
BRENDAi | 2.6.1.7, 3474 |
Reactomei | R-MMU-389661, Glyoxylate metabolism and glycine degradation R-MMU-70263, Gluconeogenesis R-MMU-8963693, Aspartate and asparagine metabolism R-MMU-8964539, Glutamate and glutamine metabolism |
Names & Taxonomyi
Protein namesi | Recommended name: Aspartate aminotransferase, mitochondrial (EC:2.6.1.1, EC:2.6.1.7)Short name: mAspAT Alternative name(s): Fatty acid-binding protein Short name: FABP-1 Glutamate oxaloacetate transaminase 2 Kynurenine aminotransferase 4 Kynurenine aminotransferase IV Kynurenine--oxoglutarate transaminase 4 Kynurenine--oxoglutarate transaminase IV Plasma membrane-associated fatty acid-binding protein Short name: FABPpm Transaminase A |
Gene namesi | Name:Got2 Synonyms:Got-2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:95792, Got2 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication
Mitochondrion
Mitochondrion
- mitochondrial inner membrane Source: MGI
- mitochondrial matrix Source: UniProtKB-SubCell
- mitochondrion Source: MGI
Plasma Membrane
- plasma membrane Source: MGI
- sarcolemma Source: MGI
- T-tubule Source: MGI
Other locations
- cell surface Source: MGI
- myelin sheath Source: UniProtKB
- perikaryon Source: MGI
- protein-containing complex Source: MGI
Keywords - Cellular componenti
Cell membrane, Membrane, MitochondrionPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 209 | Missing : Heterozygous mice are viable and healthy. Results in early lethality at homozygosity. 1 Publication | 1 | |
Mutagenesisi | 337 | R → G: Heterozygous mice are viable and healthy. Results in early lethality at homozygosity. 1 Publication | 1 |
Chemistry databases
ChEMBLi | CHEMBL3647 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 29 | MitochondrionAdd BLAST | 29 | |
ChainiPRO_0000001216 | 30 – 430 | Aspartate aminotransferase, mitochondrialAdd BLAST | 401 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 48 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 59 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 73 | N6-acetyllysine; alternateCombined sources | 1 | |
Modified residuei | 73 | N6-succinyllysine; alternateCombined sources | 1 | |
Modified residuei | 82 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 90 | N6-acetyllysine; alternateCombined sources | 1 | |
Modified residuei | 90 | N6-succinyllysine; alternateCombined sources | 1 | |
Modified residuei | 96 | 3'-nitrotyrosine; alternateCombined sources | 1 | |
Modified residuei | 96 | Phosphotyrosine; alternateBy similarity | 1 | |
Modified residuei | 107 | N6-acetyllysine; alternateCombined sources | 1 | |
Modified residuei | 107 | N6-succinyllysine; alternateCombined sources | 1 | |
Modified residuei | 122 | N6-acetyllysine; alternateCombined sources | 1 | |
Modified residuei | 122 | N6-succinyllysine; alternateCombined sources | 1 | |
Modified residuei | 143 | PhosphoserineBy similarity | 1 | |
Modified residuei | 159 | N6-acetyllysine; alternateCombined sources | 1 | |
Modified residuei | 159 | N6-succinyllysine; alternateCombined sources | 1 | |
Modified residuei | 185 | N6-acetyllysine; alternateCombined sources | 1 | |
Modified residuei | 185 | N6-succinyllysine; alternateCombined sources | 1 | |
Modified residuei | 227 | N6-succinyllysineCombined sources | 1 | |
Modified residuei | 234 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 279 | N6-(pyridoxal phosphate)lysine; alternateBy similarity | 1 | |
Modified residuei | 279 | N6-acetyllysine; alternateCombined sources | 1 | |
Modified residuei | 296 | N6-acetyllysine; alternateCombined sources | 1 | |
Modified residuei | 296 | N6-succinyllysine; alternateCombined sources | 1 | |
Modified residuei | 302 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 309 | N6-acetyllysine; alternateCombined sources | 1 | |
Modified residuei | 309 | N6-succinyllysine; alternateCombined sources | 1 | |
Modified residuei | 313 | Asymmetric dimethylarginineCombined sources | 1 | |
Modified residuei | 338 | N6-acetyllysine; alternateCombined sources | 1 | |
Modified residuei | 338 | N6-succinyllysine; alternateCombined sources | 1 | |
Modified residuei | 345 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 363 | N6-acetyllysine; alternateCombined sources | 1 | |
Modified residuei | 363 | N6-succinyllysine; alternateCombined sources | 1 | |
Modified residuei | 364 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 387 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 396 | N6-acetyllysine; alternateCombined sources | 1 | |
Modified residuei | 396 | N6-succinyllysine; alternateCombined sources | 1 | |
Modified residuei | 404 | N6-acetyllysine; alternateCombined sources | 1 | |
Modified residuei | 404 | N6-succinyllysine; alternateCombined sources | 1 |
Post-translational modificationi
Acetylation of Lys-296, Lys-345 and Lys-363 is observed in liver mitochondria from fasted mice but not from fed mice.
Keywords - PTMi
Acetylation, Methylation, Nitration, PhosphoproteinProteomic databases
CPTACi | non-CPTAC-3628 |
EPDi | P05202 |
jPOSTi | P05202 |
MaxQBi | P05202 |
PaxDbi | P05202 |
PeptideAtlasi | P05202 |
PRIDEi | P05202 |
TopDownProteomicsi | P05202 |
PTM databases
iPTMneti | P05202 |
PhosphoSitePlusi | P05202 |
SwissPalmi | P05202 |
Expressioni
Tissue specificityi
Detected in brain (at protein level).1 Publication
Gene expression databases
Bgeei | ENSMUSG00000031672, Expressed in adult mammalian kidney and 141 other tissues |
Genevisiblei | P05202, MM |
Interactioni
Subunit structurei
Homodimer.
2 PublicationsGO - Molecular functioni
- enzyme binding Source: MGI
- identical protein binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 200000, 19 interactors |
IntActi | P05202, 8 interactors |
MINTi | P05202 |
STRINGi | 10090.ENSMUSP00000034097 |
Miscellaneous databases
RNActi | P05202, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P05202 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P05202 |
Family & Domainsi
Sequence similaritiesi
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG1411, Eukaryota |
GeneTreei | ENSGT00950000183082 |
HOGENOMi | CLU_032440_1_2_1 |
InParanoidi | P05202 |
OMAi | VGACTIV |
OrthoDBi | 1104596at2759 |
PhylomeDBi | P05202 |
TreeFami | TF300641 |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
InterProi | View protein in InterPro IPR004839, Aminotransferase_I/II IPR000796, Asp_trans IPR004838, NHTrfase_class1_PyrdxlP-BS IPR015424, PyrdxlP-dep_Trfase IPR015422, PyrdxlP-dep_Trfase_dom1 IPR015421, PyrdxlP-dep_Trfase_major |
PANTHERi | PTHR11879, PTHR11879, 1 hit |
Pfami | View protein in Pfam PF00155, Aminotran_1_2, 1 hit |
PRINTSi | PR00799, TRANSAMINASE |
SUPFAMi | SSF53383, SSF53383, 1 hit |
PROSITEi | View protein in PROSITE PS00105, AA_TRANSFER_CLASS_1, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P05202-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MALLHSSRIL SGMAAAFHPG LAAAASARAS SWWTHVEMGP PDPILGVTEA
60 70 80 90 100
FKRDTNSKKM NLGVGAYRDD NGKPYVLPSV RKAEAQIAAK NLDKEYLPIG
110 120 130 140 150
GLAEFCKASA ELALGENNEV LKSGRFVTVQ TISGTGALRV GASFLQRFFK
160 170 180 190 200
FSRDVFLPKP SWGNHTPIFR DAGMQLQGYR YYDPKTCGFD FSGALEDISK
210 220 230 240 250
IPEQSVLLLH ACAHNPTGVD PRPEQWKEIA SVVKKKNLFA FFDMAYQGFA
260 270 280 290 300
SGDGDKDAWA VRHFIEQGIN VCLCQSYAKN MGLYGERVGA FTVVCKDAEE
310 320 330 340 350
AKRVESQLKI LIRPLYSNPP LNGARIAATI LTSPDLRKQW LQEVKGMADR
360 370 380 390 400
IISMRTQLVS NLKKEGSSHN WQHITDQIGM FCFTGLKPEQ VERLTKEFSV
410 420 430
YMTKDGRISV AGVTSGNVGY LAHAIHQVTK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 68 | R → G in BAE39800 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 146 | Q → E in AAB91426 (PubMed:10642497).Curated | 1 | |
Sequence conflicti | 153 | R → G in BAE39800 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 377 | Q → K in BAE39800 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 421 | L → I in BAE39800 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J02622 mRNA Translation: AAA37264.1 X06917 , X06918, X06919, X06920, X06921, X06922, X06923, X06924, X06925, X06926 Genomic DNA Translation: CAA30015.1 M37259 , M37250, M37251, M37252, M37253, M37254, M37255, M37256, M37258 Genomic DNA Translation: AAA37265.1 Sequence problems. U82470 mRNA Translation: AAB91426.1 AK136556 mRNA Translation: BAE23042.1 AK147953 mRNA Translation: BAE28248.1 AK149886 mRNA Translation: BAE29146.1 AK149926 mRNA Translation: BAE29171.1 AK150194 mRNA Translation: BAE29370.1 AK152921 mRNA Translation: BAE31596.1 AK155075 mRNA Translation: BAE33029.1 AK167767 mRNA Translation: BAE39800.1 BC089015 mRNA Translation: AAH89015.1 BC089341 mRNA Translation: AAH89341.1 |
CCDSi | CCDS22568.1 |
PIRi | S01174 |
RefSeqi | NP_034455.1, NM_010325.2 |
Genome annotation databases
Ensembli | ENSMUST00000034097; ENSMUSP00000034097; ENSMUSG00000031672 |
GeneIDi | 14719 |
KEGGi | mmu:14719 |
UCSCi | uc009mzi.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J02622 mRNA Translation: AAA37264.1 X06917 , X06918, X06919, X06920, X06921, X06922, X06923, X06924, X06925, X06926 Genomic DNA Translation: CAA30015.1 M37259 , M37250, M37251, M37252, M37253, M37254, M37255, M37256, M37258 Genomic DNA Translation: AAA37265.1 Sequence problems. U82470 mRNA Translation: AAB91426.1 AK136556 mRNA Translation: BAE23042.1 AK147953 mRNA Translation: BAE28248.1 AK149886 mRNA Translation: BAE29146.1 AK149926 mRNA Translation: BAE29171.1 AK150194 mRNA Translation: BAE29370.1 AK152921 mRNA Translation: BAE31596.1 AK155075 mRNA Translation: BAE33029.1 AK167767 mRNA Translation: BAE39800.1 BC089015 mRNA Translation: AAH89015.1 BC089341 mRNA Translation: AAH89341.1 |
CCDSi | CCDS22568.1 |
PIRi | S01174 |
RefSeqi | NP_034455.1, NM_010325.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3HLM | X-ray | 2.50 | A/B/C/D | 30-430 | [»] | |
3PD6 | X-ray | 2.40 | A/B/C/D | 30-430 | [»] | |
3PDB | X-ray | 2.40 | A/B/C/D | 30-430 | [»] | |
SMRi | P05202 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 200000, 19 interactors |
IntActi | P05202, 8 interactors |
MINTi | P05202 |
STRINGi | 10090.ENSMUSP00000034097 |
Chemistry databases
ChEMBLi | CHEMBL3647 |
PTM databases
iPTMneti | P05202 |
PhosphoSitePlusi | P05202 |
SwissPalmi | P05202 |
Proteomic databases
CPTACi | non-CPTAC-3628 |
EPDi | P05202 |
jPOSTi | P05202 |
MaxQBi | P05202 |
PaxDbi | P05202 |
PeptideAtlasi | P05202 |
PRIDEi | P05202 |
TopDownProteomicsi | P05202 |
Protocols and materials databases
Antibodypediai | 15396, 619 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000034097; ENSMUSP00000034097; ENSMUSG00000031672 |
GeneIDi | 14719 |
KEGGi | mmu:14719 |
UCSCi | uc009mzi.1, mouse |
Organism-specific databases
CTDi | 2806 |
MGIi | MGI:95792, Got2 |
Phylogenomic databases
eggNOGi | KOG1411, Eukaryota |
GeneTreei | ENSGT00950000183082 |
HOGENOMi | CLU_032440_1_2_1 |
InParanoidi | P05202 |
OMAi | VGACTIV |
OrthoDBi | 1104596at2759 |
PhylomeDBi | P05202 |
TreeFami | TF300641 |
Enzyme and pathway databases
BRENDAi | 2.6.1.7, 3474 |
Reactomei | R-MMU-389661, Glyoxylate metabolism and glycine degradation R-MMU-70263, Gluconeogenesis R-MMU-8963693, Aspartate and asparagine metabolism R-MMU-8964539, Glutamate and glutamine metabolism |
Miscellaneous databases
BioGRID-ORCSi | 14719, 2 hits in 20 CRISPR screens |
ChiTaRSi | Got2, mouse |
EvolutionaryTracei | P05202 |
PROi | PR:P05202 |
RNActi | P05202, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000031672, Expressed in adult mammalian kidney and 141 other tissues |
Genevisiblei | P05202, MM |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
InterProi | View protein in InterPro IPR004839, Aminotransferase_I/II IPR000796, Asp_trans IPR004838, NHTrfase_class1_PyrdxlP-BS IPR015424, PyrdxlP-dep_Trfase IPR015422, PyrdxlP-dep_Trfase_dom1 IPR015421, PyrdxlP-dep_Trfase_major |
PANTHERi | PTHR11879, PTHR11879, 1 hit |
Pfami | View protein in Pfam PF00155, Aminotran_1_2, 1 hit |
PRINTSi | PR00799, TRANSAMINASE |
SUPFAMi | SSF53383, SSF53383, 1 hit |
PROSITEi | View protein in PROSITE PS00105, AA_TRANSFER_CLASS_1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AATM_MOUSE | |
Accessioni | P05202Primary (citable) accession number: P05202 Secondary accession number(s): O09188 Q5HZH5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 13, 1987 |
Last sequence update: | August 13, 1987 | |
Last modified: | December 2, 2020 | |
This is version 189 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families