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Protein

Aspartate aminotransferase, cytoplasmic

Gene

Got1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H2S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain (By similarity).By similarity

Miscellaneous

In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.

Catalytic activityi

L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate.
L-cysteine + 2-oxoglutarate = mercaptopyruvate + L-glutamate.

Cofactori

Activity regulationi

Inhibited by calcium ions.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei39Aspartate; via amide nitrogenBy similarity1
Binding sitei141AspartateBy similarity1
Binding sitei195AspartateBy similarity1
Binding sitei387AspartateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminotransferase, Transferase
Biological processAmino-acid biosynthesis
LigandPyridoxal phosphate

Enzyme and pathway databases

ReactomeiR-MMU-70263 Gluconeogenesis
R-MMU-70614 Amino acid synthesis and interconversion (transamination)

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartate aminotransferase, cytoplasmic (EC:2.6.1.1, EC:2.6.1.3)
Short name:
cAspAT
Alternative name(s):
Cysteine aminotransferase, cytoplasmic
Cysteine transaminase, cytoplasmic
Short name:
cCAT
Glutamate oxaloacetate transaminase 1
Transaminase A
Gene namesi
Name:Got1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:95791 Got1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001238802 – 413Aspartate aminotransferase, cytoplasmicAdd BLAST412

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei149PhosphoserineBy similarity1
Modified residuei259N6-(pyridoxal phosphate)lysine1
Modified residuei318N6-succinyllysineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP05201
MaxQBiP05201
PaxDbiP05201
PeptideAtlasiP05201
PRIDEiP05201

2D gel databases

SWISS-2DPAGEiP05201

PTM databases

iPTMnetiP05201
PhosphoSitePlusiP05201
SwissPalmiP05201

Expressioni

Tissue specificityi

Expressed in neurons of the retina. Localizes to the inner and outer plexiform layers, the inner and outer nuclear layer and the outer segments of photoreceptors.1 Publication

Gene expression databases

BgeeiENSMUSG00000025190 Expressed in 301 organ(s), highest expression level in diaphragm
CleanExiMM_GOT1
ExpressionAtlasiP05201 baseline and differential
GenevisibleiP05201 MM

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi199999, 2 interactors
IntActiP05201, 24 interactors
MINTiP05201
STRINGi10090.ENSMUSP00000026196

Structurei

3D structure databases

ProteinModelPortaliP05201
SMRiP05201
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1411 Eukaryota
COG1448 LUCA
GeneTreeiENSGT00390000014081
HOGENOMiHOG000185898
HOVERGENiHBG000951
InParanoidiP05201
KOiK14454
OMAiELFKEWT
OrthoDBiEOG091G06G3
TreeFamiTF314089

Family and domain databases

Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 1 hit
InterProiView protein in InterPro
IPR004839 Aminotransferase_I/II
IPR000796 Asp_trans
IPR004838 NHTrfase_class1_PyrdxlP-BS
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PANTHERiPTHR11879 PTHR11879, 1 hit
PfamiView protein in Pfam
PF00155 Aminotran_1_2, 1 hit
PRINTSiPR00799 TRANSAMINASE
SUPFAMiSSF53383 SSF53383, 1 hit
PROSITEiView protein in PROSITE
PS00105 AA_TRANSFER_CLASS_1, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P05201-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAPPSVFAQV PQAPPVLVFK LTADFRDDPD PRKVNLGVGA YRTDESQPWV
60 70 80 90 100
LPVVRKVEQK IANDNSLNHE YLPILGLAEF RSCASRLVLG DNSLAIRENR
110 120 130 140 150
VGGVQSLGGT GALRIGADFL GRWYNGTDNK NTPIYVSSPT WENHNAVFSA
160 170 180 190 200
AGFKDIRPYC YWDAEKRGLD LQGFLNDLEN APEFSIFVLH ACAHNPTGTD
210 220 230 240 250
PTPEQWKQIA AVMQRRFLFP FFDSAYQGFA SGDLEKDAWA IRYFVSEGFE
260 270 280 290 300
LFCAQSFSKN FGLYNERVGN LTVVGKESDS VLRVLSQMEK IVRITWSNPP
310 320 330 340 350
AQGARIVAAT LSDPELFKEW KGNVKTMADR ILTMRSELRA RLEALKTPGT
360 370 380 390 400
WSHITEQIGM FSFTGLNPKQ VEYLVNEKHI YLLPSGRINM CGLTTKNLDY
410
VATSIHEAVT KIQ
Length:413
Mass (Da):46,248
Last modified:June 28, 2011 - v3
Checksum:i0E37C763155EA6B5
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F7ALS6F7ALS6_MOUSE
Aspartate aminotransferase, cytopla...
Got1
182Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti94L → P in AAA37263 (PubMed:3782150).Curated1
Sequence conflicti94L → P in CAA30275 (PubMed:3379636).Curated1
Sequence conflicti94L → P in AAH02057 (PubMed:15489334).Curated1
Sequence conflicti291I → N in CAA30275 (PubMed:3379636).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02623 mRNA Translation: AAA37263.1
X07302
, X07303, X07304, X07305, X07306, X07307, X07308, X07309 Genomic DNA Translation: CAA30275.1
AK146445 mRNA Translation: BAE27177.1
BC002057 mRNA Translation: AAH02057.1
CCDSiCCDS29832.1
PIRiS01076
RefSeqiNP_034454.2, NM_010324.2
UniGeneiMm.19039

Genome annotation databases

EnsembliENSMUST00000026196; ENSMUSP00000026196; ENSMUSG00000025190
GeneIDi14718
KEGGimmu:14718
UCSCiuc012bmb.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02623 mRNA Translation: AAA37263.1
X07302
, X07303, X07304, X07305, X07306, X07307, X07308, X07309 Genomic DNA Translation: CAA30275.1
AK146445 mRNA Translation: BAE27177.1
BC002057 mRNA Translation: AAH02057.1
CCDSiCCDS29832.1
PIRiS01076
RefSeqiNP_034454.2, NM_010324.2
UniGeneiMm.19039

3D structure databases

ProteinModelPortaliP05201
SMRiP05201
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199999, 2 interactors
IntActiP05201, 24 interactors
MINTiP05201
STRINGi10090.ENSMUSP00000026196

PTM databases

iPTMnetiP05201
PhosphoSitePlusiP05201
SwissPalmiP05201

2D gel databases

SWISS-2DPAGEiP05201

Proteomic databases

EPDiP05201
MaxQBiP05201
PaxDbiP05201
PeptideAtlasiP05201
PRIDEiP05201

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026196; ENSMUSP00000026196; ENSMUSG00000025190
GeneIDi14718
KEGGimmu:14718
UCSCiuc012bmb.1 mouse

Organism-specific databases

CTDi2805
MGIiMGI:95791 Got1

Phylogenomic databases

eggNOGiKOG1411 Eukaryota
COG1448 LUCA
GeneTreeiENSGT00390000014081
HOGENOMiHOG000185898
HOVERGENiHBG000951
InParanoidiP05201
KOiK14454
OMAiELFKEWT
OrthoDBiEOG091G06G3
TreeFamiTF314089

Enzyme and pathway databases

ReactomeiR-MMU-70263 Gluconeogenesis
R-MMU-70614 Amino acid synthesis and interconversion (transamination)

Miscellaneous databases

PROiPR:P05201
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000025190 Expressed in 301 organ(s), highest expression level in diaphragm
CleanExiMM_GOT1
ExpressionAtlasiP05201 baseline and differential
GenevisibleiP05201 MM

Family and domain databases

Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 1 hit
InterProiView protein in InterPro
IPR004839 Aminotransferase_I/II
IPR000796 Asp_trans
IPR004838 NHTrfase_class1_PyrdxlP-BS
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PANTHERiPTHR11879 PTHR11879, 1 hit
PfamiView protein in Pfam
PF00155 Aminotran_1_2, 1 hit
PRINTSiPR00799 TRANSAMINASE
SUPFAMiSSF53383 SSF53383, 1 hit
PROSITEiView protein in PROSITE
PS00105 AA_TRANSFER_CLASS_1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAATC_MOUSE
AccessioniPrimary (citable) accession number: P05201
Secondary accession number(s): Q3UJH8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: June 28, 2011
Last modified: November 7, 2018
This is version 162 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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