UniProtKB - P05201 (AATC_MOUSE)
Protein
Aspartate aminotransferase, cytoplasmic
Gene
Got1
Organism
Mus musculus (Mouse)
Status
Functioni
Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H2S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain (By similarity).By similarity
Miscellaneous
In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.
Catalytic activityi
Cofactori
Activity regulationi
Inhibited by calcium ions.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 39 | Aspartate; via amide nitrogenBy similarity | 1 | |
Binding sitei | 141 | AspartateBy similarity | 1 | |
Binding sitei | 195 | AspartateBy similarity | 1 | |
Binding sitei | 387 | AspartateBy similarity | 1 |
GO - Molecular functioni
- carboxylic acid binding Source: MGI
- L-aspartate:2-oxoglutarate aminotransferase activity Source: MGI
- L-cysteine:2-oxoglutarate aminotransferase activity Source: UniProtKB
- phosphatidylserine decarboxylase activity Source: MGI
- pyridoxal phosphate binding Source: InterPro
GO - Biological processi
- 2-oxoglutarate metabolic process Source: UniProtKB
- aspartate biosynthetic process Source: MGI
- aspartate catabolic process Source: MGI
- aspartate metabolic process Source: UniProtKB
- cellular response to insulin stimulus Source: Ensembl
- cellular response to mechanical stimulus Source: MGI
- dicarboxylic acid metabolic process Source: MGI
- fatty acid homeostasis Source: MGI
- glutamate catabolic process to 2-oxoglutarate Source: MGI
- glutamate catabolic process to aspartate Source: MGI
- glutamate metabolic process Source: UniProtKB
- glycerol biosynthetic process Source: MGI
- negative regulation of collagen biosynthetic process Source: MGI
- negative regulation of cytosolic calcium ion concentration Source: MGI
- negative regulation of mitochondrial depolarization Source: MGI
- Notch signaling pathway Source: MGI
- oxaloacetate metabolic process Source: MGI
- positive regulation of transforming growth factor beta receptor signaling pathway Source: MGI
- response to cadmium ion Source: Ensembl
- response to carbohydrate Source: Ensembl
- response to glucocorticoid Source: Ensembl
- response to immobilization stress Source: Ensembl
- response to transition metal nanoparticle Source: MGI
- transdifferentiation Source: MGI
Keywordsi
Molecular function | Aminotransferase, Transferase |
Biological process | Amino-acid biosynthesis |
Ligand | Pyridoxal phosphate |
Enzyme and pathway databases
Reactomei | R-MMU-70263, Gluconeogenesis R-MMU-8963693, Aspartate and asparagine metabolism |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Got1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:95791, Got1 |
Subcellular locationi
Other locations
Cytosol
- cytosol Source: MGI
Lysosome
- lysosome Source: MGI
Nucleus
- nucleoplasm Source: MGI
Other locations
- axon terminus Source: MGI
- cytoplasm Source: MGI
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000123880 | 2 – 413 | Aspartate aminotransferase, cytoplasmicAdd BLAST | 412 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 149 | PhosphoserineBy similarity | 1 | |
Modified residuei | 259 | N6-(pyridoxal phosphate)lysine | 1 | |
Modified residuei | 318 | N6-succinyllysineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | P05201 |
jPOSTi | P05201 |
MaxQBi | P05201 |
PaxDbi | P05201 |
PeptideAtlasi | P05201 |
PRIDEi | P05201 |
2D gel databases
SWISS-2DPAGEi | P05201 |
PTM databases
iPTMneti | P05201 |
PhosphoSitePlusi | P05201 |
SwissPalmi | P05201 |
Expressioni
Tissue specificityi
Expressed in neurons of the retina. Localizes to the inner and outer plexiform layers, the inner and outer nuclear layer and the outer segments of photoreceptors.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000025190, Expressed in diaphragm and 314 other tissues |
ExpressionAtlasi | P05201, baseline and differential |
Genevisiblei | P05201, MM |
Interactioni
Subunit structurei
Homodimer.
Protein-protein interaction databases
BioGRIDi | 199999, 9 interactors |
IntActi | P05201, 24 interactors |
MINTi | P05201 |
STRINGi | 10090.ENSMUSP00000026196 |
Miscellaneous databases
RNActi | P05201, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.Curated
Phylogenomic databases
eggNOGi | KOG1412, Eukaryota |
GeneTreei | ENSGT00950000183082 |
HOGENOMi | CLU_032440_1_2_1 |
InParanoidi | P05201 |
OMAi | IRSYHYW |
OrthoDBi | 1104596at2759 |
TreeFami | TF314089 |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
InterProi | View protein in InterPro IPR004839, Aminotransferase_I/II IPR000796, Asp_trans IPR004838, NHTrfase_class1_PyrdxlP-BS IPR015424, PyrdxlP-dep_Trfase IPR015422, PyrdxlP-dep_Trfase_dom1 IPR015421, PyrdxlP-dep_Trfase_major |
PANTHERi | PTHR11879, PTHR11879, 1 hit |
Pfami | View protein in Pfam PF00155, Aminotran_1_2, 1 hit |
PRINTSi | PR00799, TRANSAMINASE |
SUPFAMi | SSF53383, SSF53383, 1 hit |
PROSITEi | View protein in PROSITE PS00105, AA_TRANSFER_CLASS_1, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P05201-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAPPSVFAQV PQAPPVLVFK LTADFRDDPD PRKVNLGVGA YRTDESQPWV
60 70 80 90 100
LPVVRKVEQK IANDNSLNHE YLPILGLAEF RSCASRLVLG DNSLAIRENR
110 120 130 140 150
VGGVQSLGGT GALRIGADFL GRWYNGTDNK NTPIYVSSPT WENHNAVFSA
160 170 180 190 200
AGFKDIRPYC YWDAEKRGLD LQGFLNDLEN APEFSIFVLH ACAHNPTGTD
210 220 230 240 250
PTPEQWKQIA AVMQRRFLFP FFDSAYQGFA SGDLEKDAWA IRYFVSEGFE
260 270 280 290 300
LFCAQSFSKN FGLYNERVGN LTVVGKESDS VLRVLSQMEK IVRITWSNPP
310 320 330 340 350
AQGARIVAAT LSDPELFKEW KGNVKTMADR ILTMRSELRA RLEALKTPGT
360 370 380 390 400
WSHITEQIGM FSFTGLNPKQ VEYLVNEKHI YLLPSGRINM CGLTTKNLDY
410
VATSIHEAVT KIQ
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF7ALS6 | F7ALS6_MOUSE | Aspartate transaminase | Got1 | 182 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 94 | L → P in AAA37263 (PubMed:3782150).Curated | 1 | |
Sequence conflicti | 94 | L → P in CAA30275 (PubMed:3379636).Curated | 1 | |
Sequence conflicti | 94 | L → P in AAH02057 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 291 | I → N in CAA30275 (PubMed:3379636).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J02623 mRNA Translation: AAA37263.1 X07302 , X07303, X07304, X07305, X07306, X07307, X07308, X07309 Genomic DNA Translation: CAA30275.1 AK146445 mRNA Translation: BAE27177.1 BC002057 mRNA Translation: AAH02057.1 |
CCDSi | CCDS29832.1 |
PIRi | S01076 |
RefSeqi | NP_034454.2, NM_010324.2 |
Genome annotation databases
Ensembli | ENSMUST00000026196; ENSMUSP00000026196; ENSMUSG00000025190 |
GeneIDi | 14718 |
KEGGi | mmu:14718 |
UCSCi | uc012bmb.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J02623 mRNA Translation: AAA37263.1 X07302 , X07303, X07304, X07305, X07306, X07307, X07308, X07309 Genomic DNA Translation: CAA30275.1 AK146445 mRNA Translation: BAE27177.1 BC002057 mRNA Translation: AAH02057.1 |
CCDSi | CCDS29832.1 |
PIRi | S01076 |
RefSeqi | NP_034454.2, NM_010324.2 |
3D structure databases
SMRi | P05201 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 199999, 9 interactors |
IntActi | P05201, 24 interactors |
MINTi | P05201 |
STRINGi | 10090.ENSMUSP00000026196 |
PTM databases
iPTMneti | P05201 |
PhosphoSitePlusi | P05201 |
SwissPalmi | P05201 |
2D gel databases
SWISS-2DPAGEi | P05201 |
Proteomic databases
EPDi | P05201 |
jPOSTi | P05201 |
MaxQBi | P05201 |
PaxDbi | P05201 |
PeptideAtlasi | P05201 |
PRIDEi | P05201 |
Protocols and materials databases
Antibodypediai | 31077, 526 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000026196; ENSMUSP00000026196; ENSMUSG00000025190 |
GeneIDi | 14718 |
KEGGi | mmu:14718 |
UCSCi | uc012bmb.1, mouse |
Organism-specific databases
CTDi | 2805 |
MGIi | MGI:95791, Got1 |
Phylogenomic databases
eggNOGi | KOG1412, Eukaryota |
GeneTreei | ENSGT00950000183082 |
HOGENOMi | CLU_032440_1_2_1 |
InParanoidi | P05201 |
OMAi | IRSYHYW |
OrthoDBi | 1104596at2759 |
TreeFami | TF314089 |
Enzyme and pathway databases
Reactomei | R-MMU-70263, Gluconeogenesis R-MMU-8963693, Aspartate and asparagine metabolism |
Miscellaneous databases
BioGRID-ORCSi | 14718, 0 hits in 17 CRISPR screens |
ChiTaRSi | Got1, mouse |
PROi | PR:P05201 |
RNActi | P05201, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000025190, Expressed in diaphragm and 314 other tissues |
ExpressionAtlasi | P05201, baseline and differential |
Genevisiblei | P05201, MM |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
InterProi | View protein in InterPro IPR004839, Aminotransferase_I/II IPR000796, Asp_trans IPR004838, NHTrfase_class1_PyrdxlP-BS IPR015424, PyrdxlP-dep_Trfase IPR015422, PyrdxlP-dep_Trfase_dom1 IPR015421, PyrdxlP-dep_Trfase_major |
PANTHERi | PTHR11879, PTHR11879, 1 hit |
Pfami | View protein in Pfam PF00155, Aminotran_1_2, 1 hit |
PRINTSi | PR00799, TRANSAMINASE |
SUPFAMi | SSF53383, SSF53383, 1 hit |
PROSITEi | View protein in PROSITE PS00105, AA_TRANSFER_CLASS_1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AATC_MOUSE | |
Accessioni | P05201Primary (citable) accession number: P05201 Secondary accession number(s): Q3UJH8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 13, 1987 |
Last sequence update: | June 28, 2011 | |
Last modified: | February 10, 2021 | |
This is version 178 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families