UniProtKB - P05197 (EF2_RAT)
Elongation factor 2
Eef2
Functioni
Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 26 – 33 | GTPBy similarity | 8 | |
Nucleotide bindingi | 104 – 108 | GTPBy similarity | 5 | |
Nucleotide bindingi | 158 – 161 | GTPBy similarity | 4 |
GO - Molecular functioni
- 5S rRNA binding Source: RGD
- actin filament binding Source: RGD
- GTPase activity Source: RGD
- GTP binding Source: UniProtKB-KW
- p53 binding Source: RGD
- protein kinase binding Source: RGD
- ribosome binding Source: RGD
- RNA binding Source: RGD
- translation elongation factor activity Source: GO_Central
GO - Biological processi
- aging Source: RGD
- cellular response to brain-derived neurotrophic factor stimulus Source: RGD
- glial cell proliferation Source: RGD
- hematopoietic progenitor cell differentiation Source: RGD
- positive regulation of cytoplasmic translation Source: RGD
- positive regulation of translation Source: RGD
- response to endoplasmic reticulum stress Source: RGD
- response to estradiol Source: RGD
- response to ethanol Source: RGD
- response to folic acid Source: RGD
- response to hydrogen peroxide Source: RGD
- response to ischemia Source: RGD
- response to xenobiotic stimulus Source: RGD
- skeletal muscle cell differentiation Source: RGD
- skeletal muscle contraction Source: RGD
- translational elongation Source: RGD
Keywordsi
Molecular function | Elongation factor |
Biological process | Protein biosynthesis |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-RNO-156902, Peptide chain elongation R-RNO-5358493, Synthesis of diphthamide-EEF2 R-RNO-6798695, Neutrophil degranulation R-RNO-8876725, Protein methylation |
Names & Taxonomyi
Protein namesi | Recommended name: Elongation factor 2Short name: EF-2 |
Gene namesi | Name:Eef2 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 61979, Eef2 |
Subcellular locationi
Cytosol
- cytosol Source: RGD
Other locations
- cytoplasm Source: RGD
- membrane raft Source: RGD
- polysomal ribosome Source: RGD
- polysome Source: RGD
- ribonucleoprotein complex Source: RGD
- synapse Source: RGD
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000091004 | 2 – 858 | Elongation factor 2Add BLAST | 857 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 54 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 57 | Phosphothreonine; by EEF2KCombined sources1 Publication | 1 | |
Modified residuei | 59 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 152 | N6-succinyllysineBy similarity | 1 | |
Modified residuei | 235 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 239 | N6-acetyllysine; alternateBy similarity | 1 | |
Cross-linki | 239 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity | ||
Modified residuei | 265 | Phosphotyrosine; by CSKBy similarity | 1 | |
Modified residuei | 272 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 272 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 275 | N6-acetyllysineBy similarity | 1 | |
Cross-linki | 322 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity | ||
Modified residuei | 325 | PhosphoserineCombined sources | 1 | |
Modified residuei | 373 | Phosphotyrosine; by CSKBy similarity | 1 | |
Modified residuei | 435 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 439 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 445 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 502 | PhosphoserineCombined sources | 1 | |
Modified residuei | 525 | N6,N6,N6-trimethyllysine; by EEF2KMTBy similarity | 1 | |
Cross-linki | 529 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity | ||
Modified residuei | 572 | N6-succinyllysineBy similarity | 1 | |
Modified residuei | 595 | Phosphoserine; by CDK2By similarity | 1 | |
Modified residuei | 619 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 715 | Diphthamide1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugationProteomic databases
jPOSTi | P05197 |
PaxDbi | P05197 |
PRIDEi | P05197 |
2D gel databases
World-2DPAGEi | 0004:P05197 |
PTM databases
iPTMneti | P05197 |
PhosphoSitePlusi | P05197 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000020266, Expressed in spleen and 21 other tissues |
Genevisiblei | P05197, RN |
Interactioni
Subunit structurei
Component of the mRNA surveillance SURF complex, at least composed of ERF1, ERF3 (ERF3A or ERF3B), EEF2, UPF1/RENT1, SMG1, SMG8 and SMG9.
Interacts with RBPMS2.
By similarityGO - Molecular functioni
- actin filament binding Source: RGD
- p53 binding Source: RGD
- protein kinase binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 248199, 11 interactors |
IntActi | P05197, 9 interactors |
MINTi | P05197 |
STRINGi | 10116.ENSRNOP00000041821 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 17 – 362 | tr-type GPROSITE-ProRule annotationAdd BLAST | 346 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0469, Eukaryota |
GeneTreei | ENSGT00940000154662 |
HOGENOMi | CLU_002794_11_1_1 |
InParanoidi | P05197 |
OMAi | GVMTQTE |
OrthoDBi | 140796at2759 |
PhylomeDBi | P05197 |
TreeFami | TF300575 |
Family and domain databases
Gene3Di | 3.30.230.10, 1 hit 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR041095, EFG_II IPR035647, EFG_III/V IPR000640, EFG_V-like IPR004161, EFTu-like_2 IPR031157, G_TR_CS IPR027417, P-loop_NTPase IPR020568, Ribosomal_S5_D2-typ_fold IPR014721, Ribosomal_S5_D2-typ_fold_subgr IPR005225, Small_GTP-bd_dom IPR000795, T_Tr_GTP-bd_dom IPR009000, Transl_B-barrel_sf IPR005517, Transl_elong_EFG/EF2_IV |
Pfami | View protein in Pfam PF00679, EFG_C, 1 hit PF14492, EFG_III, 1 hit PF03764, EFG_IV, 1 hit PF00009, GTP_EFTU, 1 hit PF03144, GTP_EFTU_D2, 1 hit |
PRINTSi | PR00315, ELONGATNFCT |
SMARTi | View protein in SMART SM00838, EFG_C, 1 hit SM00889, EFG_IV, 1 hit |
SUPFAMi | SSF50447, SSF50447, 1 hit SSF52540, SSF52540, 1 hit SSF54211, SSF54211, 1 hit SSF54980, SSF54980, 2 hits |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS00301, G_TR_1, 1 hit PS51722, G_TR_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MVNFTVDQIR AIMDKKANIR NMSVIAHVDH GKSTLTDSLV CKAGIIASAR
60 70 80 90 100
AGETRFTDTR KDEQERCITI KSTAISLFYE LSENDLNFIK QSKDGSGFLI
110 120 130 140 150
NLIDSPGHVD FSSEVTAALR VTDGALVVVD CVSGVCVQTE TVLRQAIAER
160 170 180 190 200
IKPVLMMNKM DRALLELQLE PEELYQTFQR IVENVNVIIS TYGEGESGPM
210 220 230 240 250
GNIMIDPVLG TVGFGSGLHG WAFTLKQFAE MYVAKFAAKG EGQLGAAERA
260 270 280 290 300
KKVEDMMKKL WGDRYFDPAN GKFSKSANSP DGKKLPRTFC QLILDPIFKV
310 320 330 340 350
FDAIMNFRKE ETAKLIEKLD IKLDSEDKDK EGKPLLKAVM RRWLPAGDAL
360 370 380 390 400
LQMITIHLPS PVTAQKYRCE LLYEGPPDDE AAMGIKSCDP KGPLMMYISK
410 420 430 440 450
MVPTSDKGRF YAFGRVFSGV VSTGLKVRIM GPNYTPGKKE DLYLKPIQRT
460 470 480 490 500
ILMMGRYVEP IEDVPCGNIV GLVGVDQFLV KTGTITTFEH AHNMRVMKFS
510 520 530 540 550
VSPVVRVAVE AKNPADLPKL VEGLKRLAKS DPMVQCIIEE SGEHIIAGAG
560 570 580 590 600
ELHLEICLKD LEEDHACIPI KKSDPVVSYR ETVSEESNVL CLSKSPNKHN
610 620 630 640 650
RLYMKARPFP DGLAEDIDKG EVSARQELKA RARYLAEKYE WDVAEARKIW
660 670 680 690 700
CFGPDGTGPN ILTDITKGVQ YLNEIKDSVV AGFQWATKEG ALCEENMRGV
710 720 730 740 750
RFDVHDVTLH ADAIHRGGGQ IIPTARRCLY ASVLTAQPRL MEPIYLVEIQ
760 770 780 790 800
CPEQVVGGIY GVLNRKRGHV FEESQVAGTP MFVVKAYLPV NESFGFTADL
810 820 830 840 850
RSNTGGQAFP QCVFDHWQIL PGDPFDNSSR PSQVVAETRK RKGLKEGIPA
LDNFLDKL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC066661 mRNA Translation: AAH66661.1 K03502 mRNA Translation: AAA41106.1 Y07504 mRNA Translation: CAA68805.1 U75403 mRNA Translation: AAB19107.1 AF000576 mRNA Translation: AAD05363.1 |
PIRi | S04007, EFRT2 |
RefSeqi | NP_058941.1, NM_017245.2 |
Genome annotation databases
Ensembli | ENSRNOT00000047450; ENSRNOP00000041821; ENSRNOG00000020266 |
GeneIDi | 29565 |
KEGGi | rno:29565 |
UCSCi | RGD:61979, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC066661 mRNA Translation: AAH66661.1 K03502 mRNA Translation: AAA41106.1 Y07504 mRNA Translation: CAA68805.1 U75403 mRNA Translation: AAB19107.1 AF000576 mRNA Translation: AAD05363.1 |
PIRi | S04007, EFRT2 |
RefSeqi | NP_058941.1, NM_017245.2 |
3D structure databases
SMRi | P05197 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 248199, 11 interactors |
IntActi | P05197, 9 interactors |
MINTi | P05197 |
STRINGi | 10116.ENSRNOP00000041821 |
PTM databases
iPTMneti | P05197 |
PhosphoSitePlusi | P05197 |
2D gel databases
World-2DPAGEi | 0004:P05197 |
Proteomic databases
jPOSTi | P05197 |
PaxDbi | P05197 |
PRIDEi | P05197 |
Genome annotation databases
Ensembli | ENSRNOT00000047450; ENSRNOP00000041821; ENSRNOG00000020266 |
GeneIDi | 29565 |
KEGGi | rno:29565 |
UCSCi | RGD:61979, rat |
Organism-specific databases
CTDi | 1938 |
RGDi | 61979, Eef2 |
Phylogenomic databases
eggNOGi | KOG0469, Eukaryota |
GeneTreei | ENSGT00940000154662 |
HOGENOMi | CLU_002794_11_1_1 |
InParanoidi | P05197 |
OMAi | GVMTQTE |
OrthoDBi | 140796at2759 |
PhylomeDBi | P05197 |
TreeFami | TF300575 |
Enzyme and pathway databases
Reactomei | R-RNO-156902, Peptide chain elongation R-RNO-5358493, Synthesis of diphthamide-EEF2 R-RNO-6798695, Neutrophil degranulation R-RNO-8876725, Protein methylation |
Miscellaneous databases
PROi | PR:P05197 |
Gene expression databases
Bgeei | ENSRNOG00000020266, Expressed in spleen and 21 other tissues |
Genevisiblei | P05197, RN |
Family and domain databases
Gene3Di | 3.30.230.10, 1 hit 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR041095, EFG_II IPR035647, EFG_III/V IPR000640, EFG_V-like IPR004161, EFTu-like_2 IPR031157, G_TR_CS IPR027417, P-loop_NTPase IPR020568, Ribosomal_S5_D2-typ_fold IPR014721, Ribosomal_S5_D2-typ_fold_subgr IPR005225, Small_GTP-bd_dom IPR000795, T_Tr_GTP-bd_dom IPR009000, Transl_B-barrel_sf IPR005517, Transl_elong_EFG/EF2_IV |
Pfami | View protein in Pfam PF00679, EFG_C, 1 hit PF14492, EFG_III, 1 hit PF03764, EFG_IV, 1 hit PF00009, GTP_EFTU, 1 hit PF03144, GTP_EFTU_D2, 1 hit |
PRINTSi | PR00315, ELONGATNFCT |
SMARTi | View protein in SMART SM00838, EFG_C, 1 hit SM00889, EFG_IV, 1 hit |
SUPFAMi | SSF50447, SSF50447, 1 hit SSF52540, SSF52540, 1 hit SSF54211, SSF54211, 1 hit SSF54980, SSF54980, 2 hits |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS00301, G_TR_1, 1 hit PS51722, G_TR_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | EF2_RAT | |
Accessioni | P05197Primary (citable) accession number: P05197 Secondary accession number(s): P97619 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 13, 1987 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 189 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families