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Protein

Alkaline phosphatase, placental type

Gene

ALPP

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

In most mammals there are four different isozymes: placental, germ cell, intestinal and tissue non-specific (liver/bone/kidney).

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi64Magnesium4 Publications1
Metal bindingi64Zinc 14 Publications1
Active sitei114Phosphoserine intermediate1
Metal bindingi114Zinc 14 Publications1
Metal bindingi177Magnesium4 Publications1
Metal bindingi333Magnesium4 Publications1
Metal bindingi338Zinc 24 Publications1
Metal bindingi342Zinc 2; via tele nitrogen4 Publications1
Metal bindingi379Zinc 14 Publications1
Metal bindingi380Zinc 1; via tele nitrogen4 Publications1
Metal bindingi454Zinc 2; via tele nitrogen4 Publications1

GO - Molecular functioni

  • alkaline phosphatase activity Source: UniProtKB
  • magnesium ion binding Source: UniProtKB
  • zinc ion binding Source: UniProtKB

Keywordsi

Molecular functionHydrolase
LigandMagnesium, Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.1.3.1 2681
ReactomeiR-HSA-6811438 Intra-Golgi traffic
SABIO-RKiP05187

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline phosphatase, placental type (EC:3.1.3.1)
Alternative name(s):
Alkaline phosphatase Regan isozyme
Placental alkaline phosphatase 1
Short name:
PLAP-1
Gene namesi
Name:ALPP
Synonyms:PLAP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000163283.6
HGNCiHGNC:439 ALPP
MIMi171800 gene
neXtProtiNX_P05187

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei513 – 529HelicalAdd BLAST17

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi250
OpenTargetsiENSG00000163283
PharmGKBiPA24730

Chemistry databases

ChEMBLiCHEMBL4458
DrugBankiDB08413 METHYL-PHOSPHONIC ACID MONO-(4-NITRO-PHENYL) ESTER

Polymorphism and mutation databases

BioMutaiALPP
DMDMi130737

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 221 PublicationAdd BLAST22
ChainiPRO_000002403123 – 506Alkaline phosphatase, placental typeAdd BLAST484
PropeptideiPRO_0000024032507 – 535Removed in mature formAdd BLAST29

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi143 ↔ 2055 Publications
Glycosylationi144N-linked (GlcNAc...) asparagine3 Publications1
Glycosylationi271N-linked (GlcNAc...) asparagine3 Publications1
Disulfide bondi489 ↔ 4965 Publications
Lipidationi506GPI-anchor amidated aspartate1 Publication1

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

EPDiP05187
MaxQBiP05187
PaxDbiP05187
PeptideAtlasiP05187
PRIDEiP05187
ProteomicsDBi51823

PTM databases

DEPODiP05187
iPTMnetiP05187
PhosphoSitePlusiP05187

Expressioni

Tissue specificityi

Detected in placenta (at protein level).1 Publication

Gene expression databases

BgeeiENSG00000163283 Expressed in 35 organ(s), highest expression level in placenta
CleanExiHS_ALPP
GenevisibleiP05187 HS

Organism-specific databases

HPAiCAB026327
HPA038764
HPA038765
HPA051699

Interactioni

Subunit structurei

Homodimer.4 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi106751, 27 interactors
IntActiP05187, 46 interactors
MINTiP05187
STRINGi9606.ENSP00000375881

Chemistry databases

BindingDBiP05187

Structurei

Secondary structure

1535
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP05187
SMRiP05187
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP05187

Family & Domainsi

Sequence similaritiesi

Belongs to the alkaline phosphatase family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4126 Eukaryota
COG1785 LUCA
GeneTreeiENSGT00390000008704
HOGENOMiHOG000099118
HOVERGENiHBG007345
InParanoidiP05187
KOiK01077
OMAiMKNKDFM
OrthoDBiEOG091G067H
PhylomeDBiP05187
TreeFamiTF323513

Family and domain databases

CDDicd16012 ALP, 1 hit
Gene3Di3.40.720.10, 1 hit
InterProiView protein in InterPro
IPR017849 Alkaline_Pase-like_a/b/a
IPR001952 Alkaline_phosphatase
IPR018299 Alkaline_phosphatase_AS
IPR017850 Alkaline_phosphatase_core_sf
PANTHERiPTHR11596 PTHR11596, 1 hit
PfamiView protein in Pfam
PF00245 Alk_phosphatase, 1 hit
PRINTSiPR00113 ALKPHPHTASE
SMARTiView protein in SMART
SM00098 alkPPc, 1 hit
SUPFAMiSSF53649 SSF53649, 1 hit
PROSITEiView protein in PROSITE
PS00123 ALKALINE_PHOSPHATASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P05187-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLGPCMLLLL LLLGLRLQLS LGIIPVEEEN PDFWNREAAE ALGAAKKLQP
60 70 80 90 100
AQTAAKNLII FLGDGMGVST VTAARILKGQ KKDKLGPEIP LAMDRFPYVA
110 120 130 140 150
LSKTYNVDKH VPDSGATATA YLCGVKGNFQ TIGLSAAARF NQCNTTRGNE
160 170 180 190 200
VISVMNRAKK AGKSVGVVTT TRVQHASPAG TYAHTVNRNW YSDADVPASA
210 220 230 240 250
RQEGCQDIAT QLISNMDIDV ILGGGRKYMF RMGTPDPEYP DDYSQGGTRL
260 270 280 290 300
DGKNLVQEWL AKRQGARYVW NRTELMQASL DPSVTHLMGL FEPGDMKYEI
310 320 330 340 350
HRDSTLDPSL MEMTEAALRL LSRNPRGFFL FVEGGRIDHG HHESRAYRAL
360 370 380 390 400
TETIMFDDAI ERAGQLTSEE DTLSLVTADH SHVFSFGGYP LRGSSIFGLA
410 420 430 440 450
PGKARDRKAY TVLLYGNGPG YVLKDGARPD VTESESGSPE YRQQSAVPLD
460 470 480 490 500
EETHAGEDVA VFARGPQAHL VHGVQEQTFI AHVMAFAACL EPYTACDLAP
510 520 530
PAGTTDAAHP GRSVVPALLP LLAGTLLLLE TATAP
Length:535
Mass (Da):57,954
Last modified:July 1, 1989 - v2
Checksum:i13C136679A70C76B
GO

Sequence cautioni

The sequence AAA51708 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti66M → V in AAA51709 (PubMed:3461452).Curated1
Sequence conflicti261 – 262AK → GE in AAA51706 (PubMed:3001717).Curated2
Sequence conflicti277Q → R in AAA51709 (PubMed:3461452).Curated1
Sequence conflicti285T → A in AAA51709 (PubMed:3461452).Curated1
Sequence conflicti324N → H in AAA51706 (PubMed:3001717).Curated1
Sequence conflicti389Y → C in AAA51709 (PubMed:3461452).Curated1
Sequence conflicti394S → G in AAA51709 (PubMed:3461452).Curated1
Sequence conflicti396 – 397IF → FI in AAA51706 (PubMed:3001717).Curated2
Sequence conflicti401P → A in AAA51706 (PubMed:3001717).Curated1
Sequence conflicti436S → T (PubMed:3459156).Curated1

Polymorphismi

Placental ALP is highly polymorphic, there are at least three common alleles.1 Publication

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_01741925P → L2 PublicationsCorresponds to variant dbSNP:rs1130335EnsemblClinVar.1
Natural variantiVAR_05052089I → L1 PublicationCorresponds to variant dbSNP:rs13026692Ensembl.1
Natural variantiVAR_050521231R → P1 PublicationCorresponds to variant dbSNP:rs1048988Ensembl.1
Natural variantiVAR_050522263R → H1 PublicationCorresponds to variant dbSNP:rs2853378Ensembl.1
Natural variantiVAR_050523451E → G. Corresponds to variant dbSNP:rs1048994Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M19159 Genomic DNA Translation: AAA51710.1
M13077 mRNA Translation: AAC97139.1
M14169 mRNA Translation: AAA51708.1 Different initiation.
M14170 mRNA Translation: AAA51709.1
AC068134 Genomic DNA Translation: AAY24087.1
BC009647 mRNA Translation: AAH09647.1
BC068501 mRNA Translation: AAH68501.1
BC094743 mRNA Translation: AAH94743.1
M12551 mRNA Translation: AAA51706.1
CCDSiCCDS2490.1
PIRiA31074 PAHUA
RefSeqiNP_001623.3, NM_001632.4
UniGeneiHs.284255

Genome annotation databases

EnsembliENST00000392027; ENSP00000375881; ENSG00000163283
GeneIDi250
KEGGihsa:250
UCSCiuc002vsq.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

Wikipedia

Alkaline phosphatase entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M19159 Genomic DNA Translation: AAA51710.1
M13077 mRNA Translation: AAC97139.1
M14169 mRNA Translation: AAA51708.1 Different initiation.
M14170 mRNA Translation: AAA51709.1
AC068134 Genomic DNA Translation: AAY24087.1
BC009647 mRNA Translation: AAH09647.1
BC068501 mRNA Translation: AAH68501.1
BC094743 mRNA Translation: AAH94743.1
M12551 mRNA Translation: AAA51706.1
CCDSiCCDS2490.1
PIRiA31074 PAHUA
RefSeqiNP_001623.3, NM_001632.4
UniGeneiHs.284255

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EW2X-ray1.82A23-535[»]
1ZEBX-ray1.90A23-506[»]
1ZEDX-ray1.57A23-506[»]
1ZEFX-ray1.90A23-506[»]
2GLQX-ray1.60A23-506[»]
3MK0X-ray1.90A23-506[»]
3MK1X-ray1.57A23-506[»]
3MK2X-ray1.89A23-503[»]
ProteinModelPortaliP05187
SMRiP05187
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi106751, 27 interactors
IntActiP05187, 46 interactors
MINTiP05187
STRINGi9606.ENSP00000375881

Chemistry databases

BindingDBiP05187
ChEMBLiCHEMBL4458
DrugBankiDB08413 METHYL-PHOSPHONIC ACID MONO-(4-NITRO-PHENYL) ESTER

PTM databases

DEPODiP05187
iPTMnetiP05187
PhosphoSitePlusiP05187

Polymorphism and mutation databases

BioMutaiALPP
DMDMi130737

Proteomic databases

EPDiP05187
MaxQBiP05187
PaxDbiP05187
PeptideAtlasiP05187
PRIDEiP05187
ProteomicsDBi51823

Protocols and materials databases

DNASUi250
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000392027; ENSP00000375881; ENSG00000163283
GeneIDi250
KEGGihsa:250
UCSCiuc002vsq.4 human

Organism-specific databases

CTDi250
DisGeNETi250
EuPathDBiHostDB:ENSG00000163283.6
GeneCardsiALPP
HGNCiHGNC:439 ALPP
HPAiCAB026327
HPA038764
HPA038765
HPA051699
MIMi171800 gene
neXtProtiNX_P05187
OpenTargetsiENSG00000163283
PharmGKBiPA24730
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4126 Eukaryota
COG1785 LUCA
GeneTreeiENSGT00390000008704
HOGENOMiHOG000099118
HOVERGENiHBG007345
InParanoidiP05187
KOiK01077
OMAiMKNKDFM
OrthoDBiEOG091G067H
PhylomeDBiP05187
TreeFamiTF323513

Enzyme and pathway databases

BRENDAi3.1.3.1 2681
ReactomeiR-HSA-6811438 Intra-Golgi traffic
SABIO-RKiP05187

Miscellaneous databases

ChiTaRSiALPP human
EvolutionaryTraceiP05187
GeneWikiiPlacental_alkaline_phosphatase
GenomeRNAii250
PROiPR:P05187
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000163283 Expressed in 35 organ(s), highest expression level in placenta
CleanExiHS_ALPP
GenevisibleiP05187 HS

Family and domain databases

CDDicd16012 ALP, 1 hit
Gene3Di3.40.720.10, 1 hit
InterProiView protein in InterPro
IPR017849 Alkaline_Pase-like_a/b/a
IPR001952 Alkaline_phosphatase
IPR018299 Alkaline_phosphatase_AS
IPR017850 Alkaline_phosphatase_core_sf
PANTHERiPTHR11596 PTHR11596, 1 hit
PfamiView protein in Pfam
PF00245 Alk_phosphatase, 1 hit
PRINTSiPR00113 ALKPHPHTASE
SMARTiView protein in SMART
SM00098 alkPPc, 1 hit
SUPFAMiSSF53649 SSF53649, 1 hit
PROSITEiView protein in PROSITE
PS00123 ALKALINE_PHOSPHATASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPPB1_HUMAN
AccessioniPrimary (citable) accession number: P05187
Secondary accession number(s): P05188
, P06861, Q53S78, Q96DB7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: July 1, 1989
Last modified: November 7, 2018
This is version 205 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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