UniProtKB - P05187 (PPB1_HUMAN)
Protein
Alkaline phosphatase, placental type
Gene
ALPP
Organism
Homo sapiens (Human)
Status
Functioni
Miscellaneous
In most mammals there are four different isozymes: placental, germ cell, intestinal and tissue non-specific (liver/bone/kidney).
Catalytic activityi
- EC:3.1.3.1PROSITE-ProRule annotation
Cofactori
Protein has several cofactor binding sites:- Mg2+4 PublicationsNote: Binds 1 Mg2+ ion.4 Publications
- Zn2+4 PublicationsNote: Binds 2 Zn2+ ions.4 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 64 | Magnesium4 Publications | 1 | |
Metal bindingi | 64 | Zinc 14 Publications | 1 | |
Active sitei | 114 | Phosphoserine intermediate | 1 | |
Metal bindingi | 114 | Zinc 14 Publications | 1 | |
Metal bindingi | 177 | Magnesium4 Publications | 1 | |
Metal bindingi | 333 | Magnesium4 Publications | 1 | |
Metal bindingi | 338 | Zinc 24 Publications | 1 | |
Metal bindingi | 342 | Zinc 2; via tele nitrogen4 Publications | 1 | |
Metal bindingi | 379 | Zinc 14 Publications | 1 | |
Metal bindingi | 380 | Zinc 1; via tele nitrogen4 Publications | 1 | |
Metal bindingi | 454 | Zinc 2; via tele nitrogen4 Publications | 1 |
GO - Molecular functioni
- alkaline phosphatase activity Source: UniProtKB
- magnesium ion binding Source: UniProtKB
- zinc ion binding Source: UniProtKB
GO - Biological processi
- dephosphorylation Source: GO_Central
Keywordsi
Molecular function | Hydrolase |
Ligand | Magnesium, Metal-binding, Zinc |
Enzyme and pathway databases
BRENDAi | 3.1.3.1, 2681 |
PathwayCommonsi | P05187 |
Reactomei | R-HSA-6811438, Intra-Golgi traffic |
SABIO-RKi | P05187 |
Names & Taxonomyi
Protein namesi | Recommended name: Alkaline phosphatase, placental type (EC:3.1.3.1)Alternative name(s): Alkaline phosphatase Regan isozyme Placental alkaline phosphatase 1 Short name: PLAP-1 |
Gene namesi | Name:ALPP Synonyms:PLAP |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:439, ALPP |
MIMi | 171800, gene |
neXtProti | NX_P05187 |
VEuPathDBi | HostDB:ENSG00000163283.6 |
Subcellular locationi
Plasma membrane
Plasma Membrane
- plasma membrane Source: HPA
Other locations
- anchored component of membrane Source: UniProtKB-KW
- cell surface Source: UniProtKB
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 513 – 529 | HelicalAdd BLAST | 17 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 250 |
OpenTargetsi | ENSG00000163283 |
PharmGKBi | PA24730 |
Miscellaneous databases
Pharosi | P05187, Tbio |
Chemistry databases
ChEMBLi | CHEMBL4458 |
DrugBanki | DB01373, Calcium DB08413, METHYL-PHOSPHONIC ACID MONO-(4-NITRO-PHENYL) ESTER |
Genetic variation databases
BioMutai | ALPP |
DMDMi | 130737 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 22 | 1 PublicationAdd BLAST | 22 | |
ChainiPRO_0000024031 | 23 – 506 | Alkaline phosphatase, placental typeAdd BLAST | 484 | |
PropeptideiPRO_0000024032 | 507 – 535 | Removed in mature formAdd BLAST | 29 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 143 ↔ 205 | 5 Publications | ||
Glycosylationi | 144 | N-linked (GlcNAc...) asparagine3 Publications | 1 | |
Glycosylationi | 271 | N-linked (GlcNAc...) asparagine3 Publications | 1 | |
Disulfide bondi | 489 ↔ 496 | 5 Publications | ||
Lipidationi | 506 | GPI-anchor amidated aspartate1 Publication | 1 |
Keywords - PTMi
Disulfide bond, Glycoprotein, GPI-anchor, LipoproteinProteomic databases
EPDi | P05187 |
jPOSTi | P05187 |
MassIVEi | P05187 |
MaxQBi | P05187 |
PaxDbi | P05187 |
PeptideAtlasi | P05187 |
PRIDEi | P05187 |
ProteomicsDBi | 51823 |
PTM databases
DEPODi | ALPP |
GlyGeni | P05187, 2 sites |
iPTMneti | P05187 |
MetOSitei | P05187 |
PhosphoSitePlusi | P05187 |
Expressioni
Tissue specificityi
Detected in placenta (at protein level).1 Publication
Gene expression databases
Bgeei | ENSG00000163283, Expressed in placenta and 58 other tissues |
Genevisiblei | P05187, HS |
Organism-specific databases
HPAi | ENSG00000163283, Tissue enriched (placenta) |
Interactioni
Subunit structurei
Homodimer.
4 PublicationsBinary interactionsi
Hide detailsP05187
Protein-protein interaction databases
BioGRIDi | 106751, 74 interactors |
IntActi | P05187, 49 interactors |
MINTi | P05187 |
STRINGi | 9606.ENSP00000375881 |
Chemistry databases
BindingDBi | P05187 |
Miscellaneous databases
RNActi | P05187, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P05187 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P05187 |
Family & Domainsi
Sequence similaritiesi
Belongs to the alkaline phosphatase family.Curated
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG4126, Eukaryota |
GeneTreei | ENSGT00950000183063 |
HOGENOMi | CLU_008539_4_0_1 |
InParanoidi | P05187 |
OMAi | WNNKGKQ |
OrthoDBi | 454880at2759 |
PhylomeDBi | P05187 |
TreeFami | TF323513 |
Family and domain databases
CDDi | cd16012, ALP, 1 hit |
Gene3Di | 3.40.720.10, 1 hit |
InterProi | View protein in InterPro IPR001952, Alkaline_phosphatase IPR018299, Alkaline_phosphatase_AS IPR017850, Alkaline_phosphatase_core_sf |
PANTHERi | PTHR11596, PTHR11596, 1 hit |
Pfami | View protein in Pfam PF00245, Alk_phosphatase, 1 hit |
PRINTSi | PR00113, ALKPHPHTASE |
SMARTi | View protein in SMART SM00098, alkPPc, 1 hit |
SUPFAMi | SSF53649, SSF53649, 1 hit |
PROSITEi | View protein in PROSITE PS00123, ALKALINE_PHOSPHATASE, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P05187-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLGPCMLLLL LLLGLRLQLS LGIIPVEEEN PDFWNREAAE ALGAAKKLQP
60 70 80 90 100
AQTAAKNLII FLGDGMGVST VTAARILKGQ KKDKLGPEIP LAMDRFPYVA
110 120 130 140 150
LSKTYNVDKH VPDSGATATA YLCGVKGNFQ TIGLSAAARF NQCNTTRGNE
160 170 180 190 200
VISVMNRAKK AGKSVGVVTT TRVQHASPAG TYAHTVNRNW YSDADVPASA
210 220 230 240 250
RQEGCQDIAT QLISNMDIDV ILGGGRKYMF RMGTPDPEYP DDYSQGGTRL
260 270 280 290 300
DGKNLVQEWL AKRQGARYVW NRTELMQASL DPSVTHLMGL FEPGDMKYEI
310 320 330 340 350
HRDSTLDPSL MEMTEAALRL LSRNPRGFFL FVEGGRIDHG HHESRAYRAL
360 370 380 390 400
TETIMFDDAI ERAGQLTSEE DTLSLVTADH SHVFSFGGYP LRGSSIFGLA
410 420 430 440 450
PGKARDRKAY TVLLYGNGPG YVLKDGARPD VTESESGSPE YRQQSAVPLD
460 470 480 490 500
EETHAGEDVA VFARGPQAHL VHGVQEQTFI AHVMAFAACL EPYTACDLAP
510 520 530
PAGTTDAAHP GRSVVPALLP LLAGTLLLLE TATAP
Sequence cautioni
The sequence AAA51708 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 66 | M → V in AAA51709 (PubMed:3461452).Curated | 1 | |
Sequence conflicti | 261 – 262 | AK → GE in AAA51706 (PubMed:3001717).Curated | 2 | |
Sequence conflicti | 277 | Q → R in AAA51709 (PubMed:3461452).Curated | 1 | |
Sequence conflicti | 285 | T → A in AAA51709 (PubMed:3461452).Curated | 1 | |
Sequence conflicti | 324 | N → H in AAA51706 (PubMed:3001717).Curated | 1 | |
Sequence conflicti | 389 | Y → C in AAA51709 (PubMed:3461452).Curated | 1 | |
Sequence conflicti | 394 | S → G in AAA51709 (PubMed:3461452).Curated | 1 | |
Sequence conflicti | 396 – 397 | IF → FI in AAA51706 (PubMed:3001717).Curated | 2 | |
Sequence conflicti | 401 | P → A in AAA51706 (PubMed:3001717).Curated | 1 | |
Sequence conflicti | 436 | S → T (PubMed:3459156).Curated | 1 |
Polymorphismi
Placental ALP is highly polymorphic, there are at least three common alleles.1 Publication
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_017419 | 25 | P → L2 PublicationsCorresponds to variant dbSNP:rs1130335EnsemblClinVar. | 1 | |
Natural variantiVAR_050520 | 89 | I → L1 PublicationCorresponds to variant dbSNP:rs13026692Ensembl. | 1 | |
Natural variantiVAR_050521 | 231 | R → P1 PublicationCorresponds to variant dbSNP:rs1048988EnsemblClinVar. | 1 | |
Natural variantiVAR_050522 | 263 | R → H1 PublicationCorresponds to variant dbSNP:rs2853378Ensembl. | 1 | |
Natural variantiVAR_050523 | 451 | E → G. Corresponds to variant dbSNP:rs1048994Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M19159 Genomic DNA Translation: AAA51710.1 M13077 mRNA Translation: AAC97139.1 M14169 mRNA Translation: AAA51708.1 Different initiation. M14170 mRNA Translation: AAA51709.1 AC068134 Genomic DNA Translation: AAY24087.1 BC009647 mRNA Translation: AAH09647.1 BC068501 mRNA Translation: AAH68501.1 BC094743 mRNA Translation: AAH94743.1 M12551 mRNA Translation: AAA51706.1 |
CCDSi | CCDS2490.1 |
PIRi | A31074, PAHUA |
RefSeqi | NP_001623.3, NM_001632.4 |
Genome annotation databases
Ensembli | ENST00000392027; ENSP00000375881; ENSG00000163283 |
GeneIDi | 250 |
KEGGi | hsa:250 |
UCSCi | uc002vsq.4, human |
Similar proteinsi
Cross-referencesi
Web resourcesi
Wikipedia Alkaline phosphatase entry |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M19159 Genomic DNA Translation: AAA51710.1 M13077 mRNA Translation: AAC97139.1 M14169 mRNA Translation: AAA51708.1 Different initiation. M14170 mRNA Translation: AAA51709.1 AC068134 Genomic DNA Translation: AAY24087.1 BC009647 mRNA Translation: AAH09647.1 BC068501 mRNA Translation: AAH68501.1 BC094743 mRNA Translation: AAH94743.1 M12551 mRNA Translation: AAA51706.1 |
CCDSi | CCDS2490.1 |
PIRi | A31074, PAHUA |
RefSeqi | NP_001623.3, NM_001632.4 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1EW2 | X-ray | 1.82 | A | 23-535 | [»] | |
1ZEB | X-ray | 1.90 | A | 23-506 | [»] | |
1ZED | X-ray | 1.57 | A | 23-506 | [»] | |
1ZEF | X-ray | 1.90 | A | 23-506 | [»] | |
2GLQ | X-ray | 1.60 | A | 23-506 | [»] | |
3MK0 | X-ray | 1.90 | A | 23-506 | [»] | |
3MK1 | X-ray | 1.57 | A | 23-506 | [»] | |
3MK2 | X-ray | 1.89 | A | 23-503 | [»] | |
SMRi | P05187 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 106751, 74 interactors |
IntActi | P05187, 49 interactors |
MINTi | P05187 |
STRINGi | 9606.ENSP00000375881 |
Chemistry databases
BindingDBi | P05187 |
ChEMBLi | CHEMBL4458 |
DrugBanki | DB01373, Calcium DB08413, METHYL-PHOSPHONIC ACID MONO-(4-NITRO-PHENYL) ESTER |
PTM databases
DEPODi | ALPP |
GlyGeni | P05187, 2 sites |
iPTMneti | P05187 |
MetOSitei | P05187 |
PhosphoSitePlusi | P05187 |
Genetic variation databases
BioMutai | ALPP |
DMDMi | 130737 |
Proteomic databases
EPDi | P05187 |
jPOSTi | P05187 |
MassIVEi | P05187 |
MaxQBi | P05187 |
PaxDbi | P05187 |
PeptideAtlasi | P05187 |
PRIDEi | P05187 |
ProteomicsDBi | 51823 |
Protocols and materials databases
ABCDi | P05187, 14 sequenced antibodies |
Antibodypediai | 3515, 1662 antibodies |
DNASUi | 250 |
Genome annotation databases
Ensembli | ENST00000392027; ENSP00000375881; ENSG00000163283 |
GeneIDi | 250 |
KEGGi | hsa:250 |
UCSCi | uc002vsq.4, human |
Organism-specific databases
CTDi | 250 |
DisGeNETi | 250 |
GeneCardsi | ALPP |
HGNCi | HGNC:439, ALPP |
HPAi | ENSG00000163283, Tissue enriched (placenta) |
MIMi | 171800, gene |
neXtProti | NX_P05187 |
OpenTargetsi | ENSG00000163283 |
PharmGKBi | PA24730 |
VEuPathDBi | HostDB:ENSG00000163283.6 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4126, Eukaryota |
GeneTreei | ENSGT00950000183063 |
HOGENOMi | CLU_008539_4_0_1 |
InParanoidi | P05187 |
OMAi | WNNKGKQ |
OrthoDBi | 454880at2759 |
PhylomeDBi | P05187 |
TreeFami | TF323513 |
Enzyme and pathway databases
BRENDAi | 3.1.3.1, 2681 |
PathwayCommonsi | P05187 |
Reactomei | R-HSA-6811438, Intra-Golgi traffic |
SABIO-RKi | P05187 |
Miscellaneous databases
BioGRID-ORCSi | 250, 6 hits in 921 CRISPR screens |
ChiTaRSi | ALPP, human |
EvolutionaryTracei | P05187 |
GeneWikii | Placental_alkaline_phosphatase |
GenomeRNAii | 250 |
Pharosi | P05187, Tbio |
PROi | PR:P05187 |
RNActi | P05187, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000163283, Expressed in placenta and 58 other tissues |
Genevisiblei | P05187, HS |
Family and domain databases
CDDi | cd16012, ALP, 1 hit |
Gene3Di | 3.40.720.10, 1 hit |
InterProi | View protein in InterPro IPR001952, Alkaline_phosphatase IPR018299, Alkaline_phosphatase_AS IPR017850, Alkaline_phosphatase_core_sf |
PANTHERi | PTHR11596, PTHR11596, 1 hit |
Pfami | View protein in Pfam PF00245, Alk_phosphatase, 1 hit |
PRINTSi | PR00113, ALKPHPHTASE |
SMARTi | View protein in SMART SM00098, alkPPc, 1 hit |
SUPFAMi | SSF53649, SSF53649, 1 hit |
PROSITEi | View protein in PROSITE PS00123, ALKALINE_PHOSPHATASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PPB1_HUMAN | |
Accessioni | P05187Primary (citable) accession number: P05187 Secondary accession number(s): P05188 Q96DB7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 13, 1987 |
Last sequence update: | July 1, 1989 | |
Last modified: | April 7, 2021 | |
This is version 222 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 2
Human chromosome 2: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families