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UniProtKB - P05183 (CP3A2_RAT)
Protein
Cytochrome P450 3A2
Gene
Cyp3a2
Organism
Rattus norvegicus (Rat)
Status
Functioni
Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Catalytic activityi
- an organic molecule + O2 + reduced [NADPH—hemoprotein reductase] = an alcohol + H+ + H2O + oxidized [NADPH—hemoprotein reductase]EC:1.14.14.1
Cofactori
hemeBy similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 443 | Iron (heme axial ligand) | 1 |
GO - Molecular functioni
- 1-alpha,25-dihydroxyvitamin D3 23-hydroxylase activity Source: RGD
- aromatase activity Source: UniProtKB-EC
- caffeine oxidase activity Source: RGD
- demethylase activity Source: RGD
- enzyme binding Source: RGD
- estrogen 16-alpha-hydroxylase activity Source: RGD
- estrogen 2-hydroxylase activity Source: RGD
- heme binding Source: InterPro
- iron ion binding Source: RGD
- monooxygenase activity Source: RGD
- oxidoreductase activity Source: RGD
- retinoic acid 4-hydroxylase activity Source: RGD
- steroid binding Source: RGD
- steroid hydroxylase activity Source: RGD
- testosterone 6-beta-hydroxylase activity Source: RGD
- vitamin D 24-hydroxylase activity Source: RGD
- vitamin D3 25-hydroxylase activity Source: RGD
GO - Biological processi
- alkaloid catabolic process Source: RGD
- estrogen metabolic process Source: RGD
- heterocycle metabolic process Source: RGD
- lipid hydroxylation Source: RGD
- monoterpenoid metabolic process Source: RGD
- oxidative demethylation Source: RGD
- retinoic acid metabolic process Source: RGD
- retinol metabolic process Source: RGD
- steroid catabolic process Source: RGD
- steroid metabolic process Source: RGD
- vitamin D catabolic process Source: RGD
- xenobiotic catabolic process Source: RGD
- xenobiotic metabolic process Source: RGD
Keywordsi
Molecular function | Monooxygenase, Oxidoreductase |
Ligand | Heme, Iron, Metal-binding |
Enzyme and pathway databases
Reactomei | R-RNO-211981, Xenobiotics R-RNO-5423646, Aflatoxin activation and detoxification |
SABIO-RKi | P05183 |
Names & Taxonomyi
Protein namesi | Recommended name: Cytochrome P450 3A2 (EC:1.14.14.1)Alternative name(s): CYPIIIA2 Cytochrome P450-PCN2 Cytochrome P450/6-beta-A Testosterone 6-beta-hydroxylase |
Gene namesi | Name:Cyp3a2 Synonyms:Cyp3a-2, Cyp3a11 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 708379, Cyp3a2 |
Subcellular locationi
Endoplasmic reticulum
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Other locations
- cytoplasm Source: RGD
Keywords - Cellular componenti
Endoplasmic reticulum, Membrane, MicrosomePathology & Biotechi
Chemistry databases
ChEMBLi | CHEMBL3324 |
DrugBanki | DB13746, Bioallethrin DB13975, Black cohosh DB00869, Dorzolamide DB01645, Genistein DB08834, Tauroursodeoxycholic acid |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000051784 | 1 – 504 | Cytochrome P450 3A2Add BLAST | 504 |
Proteomic databases
PaxDbi | P05183 |
PTM databases
iPTMneti | P05183 |
PhosphoSitePlusi | P05183 |
Expressioni
Tissue specificityi
Expressed in liver.2 Publications
Inductioni
By pregnenolone 16-alpha-carbonitrile (PNCN) and dexamethasone.3 Publications
Family & Domainsi
Sequence similaritiesi
Belongs to the cytochrome P450 family.Curated
Phylogenomic databases
InParanoidi | P05183 |
OrthoDBi | 467733at2759 |
PhylomeDBi | P05183 |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR008072, Cyt_P450_E_CYP3A IPR002402, Cyt_P450_E_grp-II IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00464, EP450II PR01689, EP450IICYP3A PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
i Sequence
Sequence statusi: Complete.
P05183-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDLLSALTLE TWVLLAVILV LLYRLGTHRH GIFKKQGIPG PKPLPFLGTV
60 70 80 90 100
LNYYKGLGRF DMECYKKYGK IWGLFDGQTP VFAIMDTEMI KNVLVKECFS
110 120 130 140 150
VFTNRRDFGP VGIMGKAVSV AKDEEWKRYR ALLSPTFTSG RLKEMFPIIE
160 170 180 190 200
QYGDILVKYL KQEAETGKPV TMKKVFGAYS MDVITSTSFG VNVDSLNNPK
210 220 230 240 250
DPFVEKTKKL LRFDFFDPLF LSVVLFPFLT PIYEMLNICM FPKDSIAFFQ
260 270 280 290 300
KFVHRIKETR LDSKHKHRVD FLQLMLNAHN NSKDEVSHKA LSDVEIIAQS
310 320 330 340 350
VIFIFAGYET TSSTLSFVLY FLATHPDIQK KLQEEIDGAL PSKAPPTYDI
360 370 380 390 400
VMEMEYLDMV LNETLRLYPI GNRLERVCKK DIELDGLFIP KGSVVTIPTY
410 420 430 440 450
ALHHDPQHWP KPEEFHPERF SKENKGSIDP YVYLPFGNGP RNCIGMRFAL
460 470 480 490 500
MNMKLALTKV LQNFSFQPCK ETQIPLKLSR QAILEPEKPI VLKVLPRDAV
INGA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 56 – 57 | GL → AV in CAA55887 (PubMed:2043144).Curated | 2 | |
Sequence conflicti | 429 | D → H in AAA41051 (PubMed:3785219).Curated | 1 | |
Sequence conflicti | 445 | G → D in AAA41051 (PubMed:3785219).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M13646 mRNA Translation: AAA41051.1 X79319 Genomic DNA Translation: CAA55887.1 X79320 mRNA Translation: CAA55888.1 U09742 mRNA Translation: AAA82168.1 U09734 , U09725, U09726, U09727, U09728, U09729, U09730, U09731, U09732, U09733 Genomic DNA Translation: AAB60492.1 BC089765 mRNA Translation: AAH89765.1 |
PIRi | A25222 |
RefSeqi | NP_695224.2, NM_153312.2 |
Genome annotation databases
GeneIDi | 266682 |
KEGGi | rno:266682 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M13646 mRNA Translation: AAA41051.1 X79319 Genomic DNA Translation: CAA55887.1 X79320 mRNA Translation: CAA55888.1 U09742 mRNA Translation: AAA82168.1 U09734 , U09725, U09726, U09727, U09728, U09729, U09730, U09731, U09732, U09733 Genomic DNA Translation: AAB60492.1 BC089765 mRNA Translation: AAH89765.1 |
PIRi | A25222 |
RefSeqi | NP_695224.2, NM_153312.2 |
3D structure databases
SMRi | P05183 |
ModBasei | Search... |
Chemistry databases
BindingDBi | P05183 |
ChEMBLi | CHEMBL3324 |
DrugBanki | DB13746, Bioallethrin DB13975, Black cohosh DB00869, Dorzolamide DB01645, Genistein DB08834, Tauroursodeoxycholic acid |
PTM databases
iPTMneti | P05183 |
PhosphoSitePlusi | P05183 |
Proteomic databases
PaxDbi | P05183 |
Genome annotation databases
GeneIDi | 266682 |
KEGGi | rno:266682 |
Organism-specific databases
CTDi | 266682 |
RGDi | 708379, Cyp3a2 |
Phylogenomic databases
InParanoidi | P05183 |
OrthoDBi | 467733at2759 |
PhylomeDBi | P05183 |
Enzyme and pathway databases
Reactomei | R-RNO-211981, Xenobiotics R-RNO-5423646, Aflatoxin activation and detoxification |
SABIO-RKi | P05183 |
Miscellaneous databases
PROi | PR:P05183 |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR008072, Cyt_P450_E_CYP3A IPR002402, Cyt_P450_E_grp-II IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00464, EP450II PR01689, EP450IICYP3A PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CP3A2_RAT | |
Accessioni | P05183Primary (citable) accession number: P05183 Secondary accession number(s): Q5FVU5, Q64629, Q64672 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 13, 1987 |
Last sequence update: | July 15, 1999 | |
Last modified: | February 23, 2022 | |
This is version 159 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families