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Protein

Cytochrome P450 2E1

Gene

CYP2E1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. Inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms.

Catalytic activityi

4-nitrophenol + NADPH + O2 = 4-nitrocatechol + NADP+ + H2O.1 Publication

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi437Iron (heme axial ligand)1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
LigandHeme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BioCyciMetaCyc:HS05414-MONOMER
ReactomeiR-HSA-211981 Xenobiotics
R-HSA-211999 CYP2E1 reactions
R-HSA-9027307 Biosynthesis of maresin-like SPMs
SABIO-RKiP05181
SIGNORiP05181

Chemistry databases

SwissLipidsiSLP:000001596

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 2E1 (EC:1.14.13.-)
Alternative name(s):
4-nitrophenol 2-hydroxylase (EC:1.14.13.n7)
CYPIIE1
Cytochrome P450-J
Gene namesi
Name:CYP2E1
Synonyms:CYP2E
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

EuPathDBiHostDB:ENSG00000130649.9
HGNCiHGNC:2631 CYP2E1
MIMi124040 gene
neXtProtiNX_P05181

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

Pathology & Biotechi

Organism-specific databases

DisGeNETi1571
OpenTargetsiENSG00000130649
PharmGKBiPA129

Chemistry databases

ChEMBLiCHEMBL5281
DrugBankiDB00316 Acetaminophen
DB00041 Aldesleukin
DB00918 Almotriptan
DB00969 Alosetron
DB01223 Aminophylline
DB00321 Amitriptyline
DB06728 Aniline
DB01435 Antipyrine
DB00972 Azelastine
DB06770 Benzyl alcohol
DB04794 Bifonazole
DB01558 Bromazepam
DB00835 Brompheniramine
DB01156 Bupropion
DB00201 Caffeine
DB06774 Capsaicin
DB00748 Carbinoxamine
DB01136 Carvedilol
DB00477 Chlorpromazine
DB00356 Chlorzoxazone
DB00501 Cimetidine
DB00215 Citalopram
DB04920 Clevidipine
DB00636 Clofibrate
DB00882 Clomifene
DB01068 Clonazepam
DB00257 Clotrimazole
DB00363 Clozapine
DB01394 Colchicine
DB00851 Dacarbazine
DB06637 Dalfampridine
DB00250 Dapsone
DB01151 Desipramine
DB01234 Dexamethasone
DB01191 Dexfenfluramine
DB00633 Dexmedetomidine
DB00514 Dextromethorphan
DB00829 Diazepam
DB00586 Diclofenac
DB00255 Diethylstilbestrol
DB00822 Disulfiram
DB01127 Econazole
DB08846 Ellagic Acid
DB00228 Enflurane
DB00109 Enfuvirtide
DB00655 Estrone
DB00898 Ethanol
DB06689 Ethanolamine Oleate
DB00593 Ethosuximide
DB00773 Etoposide
DB01628 Etoricoxib
DB00949 Felbamate
DB08868 Fingolimod
DB01544 Flunitrazepam
DB00623 Fluphenazine
DB00690 Flurazepam
DB00176 Fluvoxamine
DB00158 Folic Acid
DB01213 Fomepizole
DB01296 Glucosamine
DB04077 Glycerol
DB05708 GTS-21
DB01159 Halothane
DB01355 Hexobarbital
DB04946 Iloperidone
DB00458 Imipramine
DB00753 Isoflurane
DB00951 Isoniazid
DB00883 Isosorbide Dinitrate
DB01167 Itraconazole
DB00170 Menadione
DB00371 Meprobamate
DB00703 Methazolamide
DB00763 Methimazole
DB01403 Methotrimeprazine
DB01028 Methoxyflurane
DB01011 Metyrapone
DB00379 Mexiletine
DB01110 Miconazole
DB00683 Midazolam
DB01204 Mitoxantrone
DB00622 Nicardipine
DB02701 Nicotinamide
DB00184 Nicotine
DB01115 Nifedipine
DB06712 Nilvadipine
DB01595 Nitrazepam
DB00540 Nortriptyline
DB00904 Ondansetron
DB01173 Orphenadrine
DB00526 Oxaliplatin
DB00617 Paramethadione
DB03783 Phenacetin
DB00780 Phenelzine
DB01174 Phenobarbital
DB01085 Pilocarpine
DB01100 Pimozide
DB04977 Plitidepsin
DB01131 Proguanil
DB00818 Propofol
DB00908 Quinidine
DB00468 Quinine
DB01045 Rifampicin
DB00503 Ritonavir
DB06201 Rufinamide
DB00118 S-Adenosylmethionine
DB01037 Selegiline
DB01236 Sevoflurane
DB00203 Sildenafil
DB00428 Streptozocin
DB00359 Sulfadiazine
DB00259 Sulfanilamide
DB00675 Tamoxifen
DB01079 Tegaserod
DB01041 Thalidomide
DB01412 Theobromine
DB00277 Theophylline
DB00599 Thiopental
DB00679 Thioridazine
DB00208 Ticlopidine
DB01007 Tioconazole
DB04858 Tirapazamine
DB05109 Trabectedin
DB00752 Tranylcypromine
DB00347 Trimethadione
DB01586 Ursodeoxycholic acid
DB00549 Zafirlukast
DB01198 Zopiclone
GuidetoPHARMACOLOGYi1330

Polymorphism and mutation databases

BioMutaiCYP2E1
DMDMi117250

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000517511 – 493Cytochrome P450 2E1Add BLAST493

Proteomic databases

PaxDbiP05181
PeptideAtlasiP05181
PRIDEiP05181
ProteomicsDBi51820

PTM databases

iPTMnetiP05181
PhosphoSitePlusiP05181

Expressioni

Inductioni

By ethanol and isoniazid.

Gene expression databases

BgeeiENSG00000130649 Expressed in 171 organ(s), highest expression level in liver
CleanExiHS_CYP2E1
ExpressionAtlasiP05181 baseline and differential
GenevisibleiP05181 HS

Organism-specific databases

HPAiHPA009128
HPA029564

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi107944, 18 interactors
IntActiP05181, 10 interactors
STRINGi9606.ENSP00000252945

Chemistry databases

BindingDBiP05181

Structurei

Secondary structure

1493
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP05181
SMRiP05181
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP05181

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni298 – 303Substrate bindingCurated6

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiKOG0156 Eukaryota
COG2124 LUCA
GeneTreeiENSGT00880000137861
HOGENOMiHOG000036992
HOVERGENiHBG015789
InParanoidiP05181
KOiK07415
OMAiFKYSDHF
OrthoDBiEOG091G0BT8
PhylomeDBiP05181
TreeFamiTF352043

Family and domain databases

Gene3Di1.10.630.10, 1 hit
InterProiView protein in InterPro
IPR001128 Cyt_P450
IPR017972 Cyt_P450_CS
IPR002401 Cyt_P450_E_grp-I
IPR008070 Cyt_P450_E_grp-I_CYP2E-like
IPR036396 Cyt_P450_sf
PfamiView protein in Pfam
PF00067 p450, 1 hit
PRINTSiPR00463 EP450I
PR01687 EP450ICYP2E
PR00385 P450
SUPFAMiSSF48264 SSF48264, 1 hit
PROSITEiView protein in PROSITE
PS00086 CYTOCHROME_P450, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All

P05181-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSALGVTVAL LVWAAFLLLV SMWRQVHSSW NLPPGPFPLP IIGNLFQLEL
60 70 80 90 100
KNIPKSFTRL AQRFGPVFTL YVGSQRMVVM HGYKAVKEAL LDYKDEFSGR
110 120 130 140 150
GDLPAFHAHR DRGIIFNNGP TWKDIRRFSL TTLRNYGMGK QGNESRIQRE
160 170 180 190 200
AHFLLEALRK TQGQPFDPTF LIGCAPCNVI ADILFRKHFD YNDEKFLRLM
210 220 230 240 250
YLFNENFHLL STPWLQLYNN FPSFLHYLPG SHRKVIKNVA EVKEYVSERV
260 270 280 290 300
KEHHQSLDPN CPRDLTDCLL VEMEKEKHSA ERLYTMDGIT VTVADLFFAG
310 320 330 340 350
TETTSTTLRY GLLILMKYPE IEEKLHEEID RVIGPSRIPA IKDRQEMPYM
360 370 380 390 400
DAVVHEIQRF ITLVPSNLPH EATRDTIFRG YLIPKGTVVV PTLDSVLYDN
410 420 430 440 450
QEFPDPEKFK PEHFLNENGK FKYSDYFKPF STGKRVCAGE GLARMELFLL
460 470 480 490
LCAILQHFNL KPLVDPKDID LSPIHIGFGC IPPRYKLCVI PRS
Length:493
Mass (Da):56,849
Last modified:April 1, 1988 - v1
Checksum:iED0399E32A005644
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H0Y593H0Y593_HUMAN
Cytochrome P450 2E1
CYP2E1
306Annotation score:
H0Y7H4H0Y7H4_HUMAN
Cytochrome P450 2E1
CYP2E1
356Annotation score:
F5H694F5H694_HUMAN
Cytochrome P450 2E1
CYP2E1
86Annotation score:
H0YGV5H0YGV5_HUMAN
Cytochrome P450 2E1
CYP2E1
43Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2Missing AA sequence (PubMed:3675576).Curated1
Sequence conflicti23W → A AA sequence (PubMed:8031147).Curated1
Sequence conflicti32L → N AA sequence (PubMed:8031147).Curated1
Sequence conflicti71Y → C in AAH67433 (PubMed:15489334).Curated1
Sequence conflicti235V → A in AAF13601 (Ref. 3) Curated1
Sequence conflicti355H → R in AAH67433 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_00836076R → H in allele CYP2E1*2; reduced activity. 1 PublicationCorresponds to variant dbSNP:rs72559710Ensembl.1
Natural variantiVAR_008361179V → I in allele CYP2E1*4. 2 PublicationsCorresponds to variant dbSNP:rs6413419Ensembl.1
Natural variantiVAR_055382219N → D1 PublicationCorresponds to variant dbSNP:rs41299426Ensembl.1
Natural variantiVAR_055383366S → C1 PublicationCorresponds to variant dbSNP:rs41299434Ensembl.1
Natural variantiVAR_008362389V → I in allele CYP2E1*3. 1 PublicationCorresponds to variant dbSNP:rs55897648Ensembl.1
Natural variantiVAR_024727457H → L2 PublicationsCorresponds to variant dbSNP:rs28969387Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02625 mRNA Translation: AAA35743.1
J02843 Genomic DNA Translation: AAA52155.1
AF182276 mRNA Translation: AAF13601.1
DQ515958 Genomic DNA Translation: ABF47105.1
AL161645 Genomic DNA No translation available.
CH471211 Genomic DNA Translation: EAW61357.1
BC067433 mRNA Translation: AAH67433.1
AF084225 mRNA Translation: AAD13753.1
D50111 Genomic DNA Translation: BAA08796.1
CCDSiCCDS7686.1
PIRiA31949
RefSeqiNP_000764.1, NM_000773.3
UniGeneiHs.12907

Genome annotation databases

EnsembliENST00000252945; ENSP00000252945; ENSG00000130649
ENST00000463117; ENSP00000440689; ENSG00000130649
GeneIDi1571
KEGGihsa:1571
UCSCiuc001lnj.1 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

Cytochrome P450 Allele Nomenclature Committee

CYP2E1 alleles

Wikipedia

CYP2E1 entry

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02625 mRNA Translation: AAA35743.1
J02843 Genomic DNA Translation: AAA52155.1
AF182276 mRNA Translation: AAF13601.1
DQ515958 Genomic DNA Translation: ABF47105.1
AL161645 Genomic DNA No translation available.
CH471211 Genomic DNA Translation: EAW61357.1
BC067433 mRNA Translation: AAH67433.1
AF084225 mRNA Translation: AAD13753.1
D50111 Genomic DNA Translation: BAA08796.1
CCDSiCCDS7686.1
PIRiA31949
RefSeqiNP_000764.1, NM_000773.3
UniGeneiHs.12907

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3E4EX-ray2.60A/B32-493[»]
3E6IX-ray2.20A/B32-493[»]
3GPHX-ray2.70A/B32-493[»]
3KOHX-ray2.90A/B32-493[»]
3LC4X-ray3.10A/B32-493[»]
3T3ZX-ray2.35A/B/C/D32-493[»]
ProteinModelPortaliP05181
SMRiP05181
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107944, 18 interactors
IntActiP05181, 10 interactors
STRINGi9606.ENSP00000252945

Chemistry databases

BindingDBiP05181
ChEMBLiCHEMBL5281
DrugBankiDB00316 Acetaminophen
DB00041 Aldesleukin
DB00918 Almotriptan
DB00969 Alosetron
DB01223 Aminophylline
DB00321 Amitriptyline
DB06728 Aniline
DB01435 Antipyrine
DB00972 Azelastine
DB06770 Benzyl alcohol
DB04794 Bifonazole
DB01558 Bromazepam
DB00835 Brompheniramine
DB01156 Bupropion
DB00201 Caffeine
DB06774 Capsaicin
DB00748 Carbinoxamine
DB01136 Carvedilol
DB00477 Chlorpromazine
DB00356 Chlorzoxazone
DB00501 Cimetidine
DB00215 Citalopram
DB04920 Clevidipine
DB00636 Clofibrate
DB00882 Clomifene
DB01068 Clonazepam
DB00257 Clotrimazole
DB00363 Clozapine
DB01394 Colchicine
DB00851 Dacarbazine
DB06637 Dalfampridine
DB00250 Dapsone
DB01151 Desipramine
DB01234 Dexamethasone
DB01191 Dexfenfluramine
DB00633 Dexmedetomidine
DB00514 Dextromethorphan
DB00829 Diazepam
DB00586 Diclofenac
DB00255 Diethylstilbestrol
DB00822 Disulfiram
DB01127 Econazole
DB08846 Ellagic Acid
DB00228 Enflurane
DB00109 Enfuvirtide
DB00655 Estrone
DB00898 Ethanol
DB06689 Ethanolamine Oleate
DB00593 Ethosuximide
DB00773 Etoposide
DB01628 Etoricoxib
DB00949 Felbamate
DB08868 Fingolimod
DB01544 Flunitrazepam
DB00623 Fluphenazine
DB00690 Flurazepam
DB00176 Fluvoxamine
DB00158 Folic Acid
DB01213 Fomepizole
DB01296 Glucosamine
DB04077 Glycerol
DB05708 GTS-21
DB01159 Halothane
DB01355 Hexobarbital
DB04946 Iloperidone
DB00458 Imipramine
DB00753 Isoflurane
DB00951 Isoniazid
DB00883 Isosorbide Dinitrate
DB01167 Itraconazole
DB00170 Menadione
DB00371 Meprobamate
DB00703 Methazolamide
DB00763 Methimazole
DB01403 Methotrimeprazine
DB01028 Methoxyflurane
DB01011 Metyrapone
DB00379 Mexiletine
DB01110 Miconazole
DB00683 Midazolam
DB01204 Mitoxantrone
DB00622 Nicardipine
DB02701 Nicotinamide
DB00184 Nicotine
DB01115 Nifedipine
DB06712 Nilvadipine
DB01595 Nitrazepam
DB00540 Nortriptyline
DB00904 Ondansetron
DB01173 Orphenadrine
DB00526 Oxaliplatin
DB00617 Paramethadione
DB03783 Phenacetin
DB00780 Phenelzine
DB01174 Phenobarbital
DB01085 Pilocarpine
DB01100 Pimozide
DB04977 Plitidepsin
DB01131 Proguanil
DB00818 Propofol
DB00908 Quinidine
DB00468 Quinine
DB01045 Rifampicin
DB00503 Ritonavir
DB06201 Rufinamide
DB00118 S-Adenosylmethionine
DB01037 Selegiline
DB01236 Sevoflurane
DB00203 Sildenafil
DB00428 Streptozocin
DB00359 Sulfadiazine
DB00259 Sulfanilamide
DB00675 Tamoxifen
DB01079 Tegaserod
DB01041 Thalidomide
DB01412 Theobromine
DB00277 Theophylline
DB00599 Thiopental
DB00679 Thioridazine
DB00208 Ticlopidine
DB01007 Tioconazole
DB04858 Tirapazamine
DB05109 Trabectedin
DB00752 Tranylcypromine
DB00347 Trimethadione
DB01586 Ursodeoxycholic acid
DB00549 Zafirlukast
DB01198 Zopiclone
GuidetoPHARMACOLOGYi1330
SwissLipidsiSLP:000001596

PTM databases

iPTMnetiP05181
PhosphoSitePlusiP05181

Polymorphism and mutation databases

BioMutaiCYP2E1
DMDMi117250

Proteomic databases

PaxDbiP05181
PeptideAtlasiP05181
PRIDEiP05181
ProteomicsDBi51820

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000252945; ENSP00000252945; ENSG00000130649
ENST00000463117; ENSP00000440689; ENSG00000130649
GeneIDi1571
KEGGihsa:1571
UCSCiuc001lnj.1 human

Organism-specific databases

CTDi1571
DisGeNETi1571
EuPathDBiHostDB:ENSG00000130649.9
GeneCardsiCYP2E1
HGNCiHGNC:2631 CYP2E1
HPAiHPA009128
HPA029564
MIMi124040 gene
neXtProtiNX_P05181
OpenTargetsiENSG00000130649
PharmGKBiPA129
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0156 Eukaryota
COG2124 LUCA
GeneTreeiENSGT00880000137861
HOGENOMiHOG000036992
HOVERGENiHBG015789
InParanoidiP05181
KOiK07415
OMAiFKYSDHF
OrthoDBiEOG091G0BT8
PhylomeDBiP05181
TreeFamiTF352043

Enzyme and pathway databases

BioCyciMetaCyc:HS05414-MONOMER
ReactomeiR-HSA-211981 Xenobiotics
R-HSA-211999 CYP2E1 reactions
R-HSA-9027307 Biosynthesis of maresin-like SPMs
SABIO-RKiP05181
SIGNORiP05181

Miscellaneous databases

ChiTaRSiCYP2E1 human
EvolutionaryTraceiP05181
GeneWikiiCYP2E1
GenomeRNAii1571
PROiPR:P05181
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000130649 Expressed in 171 organ(s), highest expression level in liver
CleanExiHS_CYP2E1
ExpressionAtlasiP05181 baseline and differential
GenevisibleiP05181 HS

Family and domain databases

Gene3Di1.10.630.10, 1 hit
InterProiView protein in InterPro
IPR001128 Cyt_P450
IPR017972 Cyt_P450_CS
IPR002401 Cyt_P450_E_grp-I
IPR008070 Cyt_P450_E_grp-I_CYP2E-like
IPR036396 Cyt_P450_sf
PfamiView protein in Pfam
PF00067 p450, 1 hit
PRINTSiPR00463 EP450I
PR01687 EP450ICYP2E
PR00385 P450
SUPFAMiSSF48264 SSF48264, 1 hit
PROSITEiView protein in PROSITE
PS00086 CYTOCHROME_P450, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCP2E1_HUMAN
AccessioniPrimary (citable) accession number: P05181
Secondary accession number(s): Q5VZD5, Q6NWT9, Q9UK47
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: April 1, 1988
Last modified: November 7, 2018
This is version 195 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
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