ISG15

Functionⁱ

Ubiquitin-like protein which plays a key role in the innate immune response to viral infection either via its conjugation to a target protein (ISGylation) or via its action as a free or unconjugated protein. ISGylation involves a cascade of enzymatic reactions involving E1, E2, and E3 enzymes which catalyze the conjugation of ISG15 to a lysine residue in the target protein. Its target proteins include IFIT1, MX1/MxA, PPM1B, UBE2L6, UBA7, CHMP5, CHMP2A, CHMP4B and CHMP6. Can also isgylate: EIF2AK2/PKR which results in its activation, DDX58/RIG-I which inhibits its function in antiviral signaling response, EIF4E2 which enhances its cap structure-binding activity and translation-inhibition activity, UBE2N and UBE2E1 which negatively regulates their activity, IRF3 which inhibits its ubiquitination and degradation and FLNB which prevents its ability to interact with the upstream activators of the JNK cascade therby inhibiting IFNA-induced JNK signaling. Exhibits antiviral activity towards both DNA and RNA viruses, including influenza A, HIV-1 and Ebola virus. Restricts HIV-1 and ebola virus via disruption of viral budding. Inhibits the ubiquitination of HIV-1 Gag and host TSG101 and disrupts their interaction, thereby preventing assembly and release of virions from infected cells. Inhibits Ebola virus budding mediated by the VP40 protein by disrupting ubiquitin ligase activity of NEDD4 and its ability to ubiquitinate VP40. ISGylates influenza A virus NS1 protein which causes a loss of function of the protein and the inhibition of virus replication. The secreted form of ISG15 can: induce natural killer cell proliferation, act as a chemotactic factor for neutrophils and act as a IFN-gamma-inducing cytokine playing an essential role in antimycobacterial immunity.19 Publications

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Binding siteⁱ	153	Activating enzymeBy similarity		1

GO - Molecular functionⁱ

protein tag Source: Ensembl

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

defense response to bacterium
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 22859821
defense response to virus
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 19357168
ISG15-protein conjugation
Source: UniProtKB
Inferred from direct assayⁱ
- 21543490
modification-dependent protein catabolic process Source: Ensembl
negative regulation of protein ubiquitination
Source: UniProtKB
Inferred from direct assayⁱ
- 18305167
negative regulation of type I interferon production Source: Reactome
negative regulation of viral genome replication
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 19357168
positive regulation of bone mineralization Source: UniProtKB
positive regulation of erythrocyte differentiation Source: Ensembl
regulation of interferon-gamma production
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 22859821
response to type I interferon
Source: UniProtKB
Inferred from direct assayⁱ
- 22859821
translesion synthesis Source: Reactome
type I interferon signaling pathway Source: Reactome
viral process Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Antiviral defense, Host-virus interaction, Immunity, Innate immunity, Ubl conjugation pathway

Biological process

Antiviral defense, Host-virus interaction, Immunity, Innate immunity, Ubl conjugation pathway

Enzyme and pathway databases

Reactomeⁱ	R-HSA-1169408 ISG15 antiviral mechanism R-HSA-168276 NS1 Mediated Effects on Host Pathways R-HSA-168928 DDX58/IFIH1-mediated induction of interferon-alpha/beta R-HSA-5656169 Termination of translesion DNA synthesis R-HSA-909733 Interferon alpha/beta signaling R-HSA-936440 Negative regulators of DDX58/IFIH1 signaling

Reactomeⁱ

R-HSA-1169408 ISG15 antiviral mechanism
R-HSA-168276 NS1 Mediated Effects on Host Pathways
R-HSA-168928 DDX58/IFIH1-mediated induction of interferon-alpha/beta
R-HSA-5656169 Termination of translesion DNA synthesis
R-HSA-909733 Interferon alpha/beta signaling
R-HSA-936440 Negative regulators of DDX58/IFIH1 signaling

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Ubiquitin-like protein ISG15 Alternative name(s): Interferon-induced 15 kDa protein Interferon-induced 17 kDa protein Short name: IP17 Ubiquitin cross-reactive protein Short name: hUCRP
Gene namesⁱ	Name:ISG15 Synonyms:G1P2, UCRP
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 1

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000187608.8
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:4053 ISG15
MIMⁱ	147571 gene
neXtProtⁱ	NX_P05161

Keywords - Cellular componentⁱ

Cytoplasm, Secreted

Pathology & Biotechⁱ

Involvement in diseaseⁱ

Immunodeficiency 38, with basal ganglia calcification (IMD38)1 Publication

The disease is caused by mutations affecting the gene represented in this entry.

Disease descriptionA primary immunodeficiency predisposing individuals to severe clinical disease upon infection with weakly virulent mycobacteria, including Mycobacterium bovis Bacille Calmette-Guerin (BCG) vaccines. Patients are also susceptible to Salmonella and Mycobacterium tubercolosis infections. Affected individuals have intracranial calcification.

Organism-specific databases

DisGeNET More...DisGeNETⁱ	9636
MalaCards human disease database More...MalaCardsⁱ	ISG15
MIMⁱ	616126 phenotype
Open Targets More...OpenTargetsⁱ	ENSG00000187608
Orphanetⁱ	319563 Mendelian susceptibility to mycobacterial diseases due to complete ISG15 deficiency
PharmGKBⁱ	PA28465

Polymorphism and mutation databases

BioMutaⁱ	ISG15
DMDMⁱ	52001470

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Length
Initiator methionineⁱ		Removed1 Publication
ChainⁱPRO_0000035986	2 – 157	Ubiquitin-like protein ISG15Add BLAST	156
PropeptideⁱPRO_0000035987	158 – 165	Removed in mature form	8

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	78	S-nitrosocysteine; alternate1 Publication	1
Disulfide bondⁱ	78	Interchain (with C-87 in UBE2N); alternate1 Publication
Cross-linkⁱ	157	Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)PROSITE-ProRule annotation

Post-translational modificationⁱ

S-nitrosylation decreases its dimerization, thereby increasing the availability as well as the solubility of monomeric ISG15 for its conjugation to cellular proteins.1 Publication

Induced as an inactive, precursor protein that is cleaved by specific proteases to expose the C-terminal diglycine (LRLRGG) motif. This motif is essential not only for its conjugation to substrates but also for its recognition by the relevant processing proteases.2 Publications

Keywords - PTMⁱ

Disulfide bond, Isopeptide bond, S-nitrosylation

Proteomic databases

EPDⁱ	P05161
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P05161
PaxDbⁱ	P05161
PeptideAtlas More...PeptideAtlasⁱ	P05161
PRIDEⁱ	P05161
ProteomicsDBⁱ	51809
TopDownProteomicsⁱ	P05161

PTM databases

iPTMnetⁱ	P05161
PhosphoSitePlusⁱ	P05161

Expressionⁱ

Tissue specificityⁱ

Detected in lymphoid cells, striated and smooth muscle, several epithelia and neurons. Expressed in neutrophils, monocytes and lymphocytes. Enhanced expression seen in pancreatic adenocarcinoma, endometrial cancer, and bladder cancer, as compared to non-cancerous tissue. In bladder cancer, the increase in expression exhibits a striking positive correlation with more advanced stages of the disease.2 Publications

Inductionⁱ

Gene expression databases

Bgeeⁱ	ENSG00000187608 Expressed in 228 organ(s), highest expression level in epithelium of bronchus
CleanExⁱ	HS_ISG15
ExpressionAtlasⁱ	P05161 baseline and differential
Genevisibleⁱ	P05161 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	HPA004627

HPAⁱ

HPA004627

Interactionⁱ

Subunit structureⁱ

Homodimer; disulfide-linked. Interacts with, and is conjugated to its targets by the UBE1L (E1 enzyme) and UBE2E2 (E2 enzyme) (Probable). Interacts with NEDD4.Curated3 Publications

(Microbial infection) Interacts with vaccinia virus protein E3.1 Publication

(Microbial infection) Interaction with influenza B NS1 protein inhibits its conjugation.1 Publication

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
FLNB	O75369	4	EBI-746466,EBI-352089
L	Q6TQR6	5	EBI-746466,EBI-4403908	From Crimean-Congo hemorrhagic fever virus (strain Nigeria/IbAr10200/1970).
NS	P03495	4	EBI-746466,EBI-2548993	From Influenza A virus (strain A/Udorn/307/1972 H3N2).
NS	P03502	4	EBI-746466,EBI-15938710	From Influenza B virus (strain B/Lee/1940).
PPM1B	O75688	2	EBI-746466,EBI-1047039
UBA7	P41226	11	EBI-746466,EBI-751921
USP18	Q9UMW8	9	EBI-746466,EBI-356206

Protein-protein interaction databases

BioGridⁱ	114995, 188 interactors
Database of interacting proteins More...DIPⁱ	DIP-29814N
IntActⁱ	P05161, 41 interactors
Molecular INTeraction database More...MINTⁱ	P05161
STRINGⁱ	9606.ENSP00000368699

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	4 – 9	Combined sources	6
Beta strandⁱ	14 – 18	Combined sources	5
Helixⁱ	25 – 36	Combined sources	12
Helixⁱ	40 – 42	Combined sources	3
Beta strandⁱ	43 – 48	Combined sources	6
Beta strandⁱ	56 – 58	Combined sources	3
Helixⁱ	60 – 63	Combined sources	4
Beta strandⁱ	70 – 75	Combined sources	6
Beta strandⁱ	82 – 87	Combined sources	6
Beta strandⁱ	93 – 98	Combined sources	6
Helixⁱ	104 – 115	Combined sources	12
Helixⁱ	119 – 121	Combined sources	3
Beta strandⁱ	122 – 126	Combined sources	5
Beta strandⁱ	129 – 131	Combined sources	3
Helixⁱ	137 – 140	Combined sources	4
Beta strandⁱ	147 – 152	Combined sources	6

Show more details

3D structure databases

ProteinModelPortalⁱ	P05161
SMRⁱ	P05161
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	P05161

EvolutionaryTraceⁱ

P05161

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	2 – 78	Ubiquitin-like 1PROSITE-ProRule annotationAdd BLAST		77
Domainⁱ	79 – 157	Ubiquitin-like 2PROSITE-ProRule annotationAdd BLAST		79

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	153 – 157	Involved in the ligation of specific target proteinsBy similarity		5

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	152 – 157	LRLRGG		6

Domainⁱ

Both the Ubiquitin-like 1 and Ubiquitin-like 2 domains are required for its efficient conjugation to cellular proteins. The two domains play different roles in the ISGylation pathway: Ubiquitin-like 2 domain is necessary for the first two steps allowing the linking of ISG15 to the E1 and E2 enzymes while Ubiquitin-like 1 domain is essential for the final, E3-mediated transfer of ISG15, from the E2 to the Lys of the target protein (PubMed:18356159).1 Publication

Keywords - Domainⁱ

Repeat

Phylogenomic databases

eggNOGⁱ	KOG0001 Eukaryota COG5272 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00810000125435
HOGENOMⁱ	HOG000233942
HOVERGENⁱ	HBG000057
InParanoidⁱ	P05161
KEGG Orthology (KO) More...KOⁱ	K12159
OMAⁱ	CTVYMNL
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G178I
PhylomeDBⁱ	P05161
TreeFamⁱ	TF338379

Family and domain databases

InterProⁱ	View protein in InterPro IPR019956 Ubiquitin IPR029071 Ubiquitin-like_domsf IPR000626 Ubiquitin_dom
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00240 ubiquitin, 2 hits
PRINTSⁱ	PR00348 UBIQUITIN
SMARTⁱ	View protein in SMART SM00213 UBQ, 2 hits
SUPFAMⁱ	SSF54236 SSF54236, 2 hits
PROSITEⁱ	View protein in PROSITE PS50053 UBIQUITIN_2, 2 hits

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show all Align All

P05161-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MGWDLTVKML AGNEFQVSLS SSMSVSELKA QITQKIGVHA FQQRLAVHPS 
        60         70         80         90        100
GVALQDRVPL ASQGLGPGST VLLVVDKCDE PLSILVRNNK GRSSTYEVRL 
       110        120        130        140        150
TQTVAHLKQQ VSGLEGVQDD LFWLTFEGKP LEDQLPLGEY GLKPLSTVFM 
       160 
NLRLRGGGTE PGGRS

Length:165

Mass (Da):17,888

Last modified:January 23, 2007 - v5

Checksum:ⁱB6858A15AB0FFFDE

GO

Computationally mapped potential isoform sequencesⁱ

There are 2 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

A0A096LPJ4	A0A096LPJ4_HUMAN	Ubiquitin-like protein ISG15 Ubiquitin-like protein ISG15	ISG15	157	Annotation score:
A0A096LNZ9	A0A096LNZ9_HUMAN	Ubiquitin-like protein ISG15 Ubiquitin-like protein ISG15	ISG15	143	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	35	K → N in AAA36128 (PubMed:3476954).Curated		1

Natural variant

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Natural variantⁱVAR_016181	83	S → N3 PublicationsCorresponds to variant dbSNP:rs1921EnsemblClinVar.		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M13755 mRNA Translation: AAA36038.1 M21786 Genomic DNA Translation: AAA36128.1 AY168648 mRNA Translation: AAN86983.1 BT007297 mRNA Translation: AAP35961.1 AL645608 Genomic DNA No translation available. CH471183 Genomic DNA Translation: EAW56295.1 BC009507 mRNA Translation: AAH09507.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS6.1
PIRⁱ	A28304 A28138
NCBI Reference Sequences More...RefSeqⁱ	NP_005092.1, NM_005101.3
UniGeneⁱ	Hs.458485

Genome annotation databases

Ensemblⁱ	ENST00000379389; ENSP00000368699; ENSG00000187608
GeneIDⁱ	9636
KEGGⁱ	hsa:9636
UCSC genome browser More...UCSCⁱ	uc001acj.5 human

Keywords - Coding sequence diversityⁱ

Polymorphism

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P05161	Ubiquitin-like protein ISG15		HUMAN		157	UniRef100_P05161
Ubiquitin-like protein ISG15 (Fragment)		HUMAN		143

Protein	Similar proteins		Species	Score	Length	Source
P05161	Ubiquitin-like protein ISG15		HUMAN		157	UniRef90_P05161
Uncharacterized protein		RHIRO		167
Uncharacterized protein		RHIBE		167
Uncharacterized protein (Fragment)		MACFA		165
Ubiquitin-like protein ISG15		MACMU		165
+23

Protein	Similar proteins		Species	Score	Length	Source
P05161	Ubiquitin-like protein ISG15	)	BOVIN		154	UniRef50_P05161
Ubiquitin-like protein ISG15		HUMAN		157
Uncharacterized protein		RHIRO		167
Uncharacterized protein		RHIBE		167
Uncharacterized protein		PANPA		165
+58

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M13755 mRNA Translation: AAA36038.1 M21786 Genomic DNA Translation: AAA36128.1 AY168648 mRNA Translation: AAN86983.1 BT007297 mRNA Translation: AAP35961.1 AL645608 Genomic DNA No translation available. CH471183 Genomic DNA Translation: EAW56295.1 BC009507 mRNA Translation: AAH09507.1
CCDSⁱ	CCDS6.1
PIRⁱ	A28304 A28138
RefSeqⁱ	NP_005092.1, NM_005101.3
UniGeneⁱ	Hs.458485

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1Z2M	X-ray	2.50	A	1-155	[»]
	2HJ8	NMR	-	A	79-157	[»]
	3PHX	X-ray	1.60	B	79-156	[»]
	3PSE	X-ray	2.30	B	1-156	[»]
	3R66	X-ray	2.30	C/D	1-157	[»]
	3RT3	X-ray	2.01	B	1-158	[»]
	3SDL	X-ray	2.29	C/D	1-157	[»]
	5TL6	X-ray	2.62	A/C	80-157	[»]
	5W8T	X-ray	2.76	B/D	80-156	[»]
	5W8U	X-ray	2.41	B/D	80-156	[»]
	6FFA	X-ray	1.50	B	79-155	[»]
ProteinModelPortalⁱ	P05161
SMRⁱ	P05161
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	114995, 188 interactors
DIPⁱ	DIP-29814N
IntActⁱ	P05161, 41 interactors
MINTⁱ	P05161
STRINGⁱ	9606.ENSP00000368699

PTM databases

iPTMnetⁱ	P05161
PhosphoSitePlusⁱ	P05161

Polymorphism and mutation databases

BioMutaⁱ	ISG15
DMDMⁱ	52001470

Proteomic databases

EPDⁱ	P05161
MaxQBⁱ	P05161
PaxDbⁱ	P05161
PeptideAtlasⁱ	P05161
PRIDEⁱ	P05161
ProteomicsDBⁱ	51809
TopDownProteomicsⁱ	P05161

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	9636
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000379389; ENSP00000368699; ENSG00000187608
GeneIDⁱ	9636
KEGGⁱ	hsa:9636
UCSCⁱ	uc001acj.5 human

Organism-specific databases

CTDⁱ	9636
DisGeNETⁱ	9636
EuPathDBⁱ	HostDB:ENSG00000187608.8
GeneCardsⁱ	ISG15
HGNCⁱ	HGNC:4053 ISG15
HPAⁱ	HPA004627
MalaCardsⁱ	ISG15
MIMⁱ	147571 gene 616126 phenotype
neXtProtⁱ	NX_P05161
OpenTargetsⁱ	ENSG00000187608
Orphanetⁱ	319563 Mendelian susceptibility to mycobacterial diseases due to complete ISG15 deficiency
PharmGKBⁱ	PA28465
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG0001 Eukaryota COG5272 LUCA
GeneTreeⁱ	ENSGT00810000125435
HOGENOMⁱ	HOG000233942
HOVERGENⁱ	HBG000057
InParanoidⁱ	P05161
KOⁱ	K12159
OMAⁱ	CTVYMNL
OrthoDBⁱ	EOG091G178I
PhylomeDBⁱ	P05161
TreeFamⁱ	TF338379

Enzyme and pathway databases

Reactomeⁱ	R-HSA-1169408 ISG15 antiviral mechanism R-HSA-168276 NS1 Mediated Effects on Host Pathways R-HSA-168928 DDX58/IFIH1-mediated induction of interferon-alpha/beta R-HSA-5656169 Termination of translesion DNA synthesis R-HSA-909733 Interferon alpha/beta signaling R-HSA-936440 Negative regulators of DDX58/IFIH1 signaling

Reactomeⁱ

R-HSA-1169408 ISG15 antiviral mechanism
R-HSA-168276 NS1 Mediated Effects on Host Pathways
R-HSA-168928 DDX58/IFIH1-mediated induction of interferon-alpha/beta
R-HSA-5656169 Termination of translesion DNA synthesis
R-HSA-909733 Interferon alpha/beta signaling
R-HSA-936440 Negative regulators of DDX58/IFIH1 signaling

Miscellaneous databases

ChiTaRSⁱ	ISG15 human
EvolutionaryTraceⁱ	P05161
GeneWikiⁱ	ISG15
GenomeRNAiⁱ	9636
Protein Ontology More...PROⁱ	PR:P05161
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000187608 Expressed in 228 organ(s), highest expression level in epithelium of bronchus
CleanExⁱ	HS_ISG15
ExpressionAtlasⁱ	P05161 baseline and differential
Genevisibleⁱ	P05161 HS

Family and domain databases

InterProⁱ	View protein in InterPro IPR019956 Ubiquitin IPR029071 Ubiquitin-like_domsf IPR000626 Ubiquitin_dom
Pfamⁱ	View protein in Pfam PF00240 ubiquitin, 2 hits
PRINTSⁱ	PR00348 UBIQUITIN
SMARTⁱ	View protein in SMART SM00213 UBQ, 2 hits
SUPFAMⁱ	SSF54236 SSF54236, 2 hits
PROSITEⁱ	View protein in PROSITE PS50053 UBIQUITIN_2, 2 hits
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	ISG15_HUMAN
Accessionⁱ	P05161Primary (citable) accession number: P05161 Secondary accession number(s): Q5SVA4, Q7Z2G2, Q96GF0
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
	Last sequence update:	January 23, 2007
	Last modified:	November 7, 2018
	This is version 207 of the entry and version 5 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM
Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations
Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
PDB cross-references
Index of Protein Data Bank (PDB) cross-references

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UniProtKB - P05161 (ISG15_HUMAN)

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Ubiquitin-like protein ISG15

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted

Other locations

Cytosol

Extracellular region or secreted

Nucleus

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Organism-specific databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

Motif

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Natural variant

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

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<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Keywords - Domainⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ