Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Phosphoenolpyruvate carboxykinase, cytosolic [GTP]

Gene

PCK1

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.By similarity

Miscellaneous

In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one.

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.By similarity

Cofactori

Mn2+By similarityNote: Binds 1 Mn2+ ion per subunit.By similarity

Activity regulationi

Activity is affected by a number of hormones regulating this metabolic process (such as glucagon, insulin, or glucocorticoids).

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.By similarity
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei87SubstrateBy similarity1
Metal bindingi244ManganeseBy similarity1
Metal bindingi264Manganese; via tele nitrogenBy similarity1
Binding sitei286SubstrateBy similarity1
Active sitei288By similarity1
Metal bindingi311ManganeseBy similarity1
Binding sitei405GTPBy similarity1
Binding sitei436GTPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi287 – 292GTPBy similarity6
Nucleotide bindingi530 – 533GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDecarboxylase, Lyase
Biological processGluconeogenesis
LigandGTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-GGA-352875 Gluconeogenesis
SABIO-RKiP05153
UniPathwayi
UPA00138

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase, cytosolic [GTP] (EC:4.1.1.32By similarity)
Short name:
PEPCK-C
Gene namesi
Name:PCK1By similarity
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001036311 – 622Phosphoenolpyruvate carboxykinase, cytosolic [GTP]Add BLAST622

Proteomic databases

PaxDbiP05153
PRIDEiP05153

Interactioni

Subunit structurei

Monomer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9031.ENSGALP00000012342

Structurei

3D structure databases

ProteinModelPortaliP05153
SMRiP05153
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni235 – 237Substrate bindingBy similarity3
Regioni403 – 405Substrate bindingBy similarity3

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3749 Eukaryota
COG1274 LUCA
HOGENOMiHOG000191700
HOVERGENiHBG053651
InParanoidiP05153
KOiK01596
PhylomeDBiP05153

Family and domain databases

CDDicd00819 PEPCK_GTP, 1 hit
Gene3Di3.40.449.10, 2 hits
3.90.228.20, 2 hits
HAMAPiMF_00452 PEPCK_GTP, 1 hit
InterProiView protein in InterPro
IPR018091 PEP_carboxykin_GTP_CS
IPR013035 PEP_carboxykinase_C
IPR008209 PEP_carboxykinase_GTP
IPR035077 PEP_carboxykinase_GTP_C
IPR035078 PEP_carboxykinase_GTP_N
IPR008210 PEP_carboxykinase_N
PANTHERiPTHR11561 PTHR11561, 1 hit
PfamiView protein in Pfam
PF00821 PEPCK_C, 1 hit
PF17297 PEPCK_N, 1 hit
PIRSFiPIRSF001348 PEP_carboxykinase_GTP, 1 hit
SUPFAMiSSF68923 SSF68923, 1 hit
PROSITEiView protein in PROSITE
PS00505 PEPCK_GTP, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.iShow all

P05153-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAPELKTEVN IISKVIQGDL ESLPPQVREF IESNAKLCQP ESIHICDGSE
60 70 80 90 100
EENKKILDIM VEQGMIKKLS KYENCWLALT NPRDVARIES KTVIITQEQR
110 120 130 140 150
DTIPIPKTGS SQLGRWMSEE DFEKAFNTRF PGCMQGRTMY VIPFSMGPIG
160 170 180 190 200
SPLAKIGIEL TDSPYVVASM RMMTRMGTAA LKALGNGEFV KCLHSVGCPL
210 220 230 240 250
PLKEPLINNW PCNPELTLIA HLPDRREIIS FGSGYGGNSL LGKKCFALRI
260 270 280 290 300
ASRIAKEEGW LAEHMLILGI TNPEGEKKYF AAAFPSACGK TNLAMMNPSR
310 320 330 340 350
PGWKIECVGD DIAWMKFDEL GNLRAINPEN GFFGVAPGTS IKTNPNAIKT
360 370 380 390 400
IFKNTIFTNV AETSDGGVYW EGIDEPLPPG VTLTSWKNKD WTPDNGEPCA
410 420 430 440 450
HPNSRFCTPA SQCPIMDPAW ESPEGVPIEG IIFGGRRPAG VPLVYEAFNW
460 470 480 490 500
QHGVFIGAAM RSEATAAAEH KGKIIMHDPF AMRPFFGYNF GKYLAHWLSM
510 520 530 540 550
AHRPAAKLPR IFHVNWFRKD SQGKFLWPGY GENSRVLEWM FNRIQGKASA
560 570 580 590 600
KSTAIGYIPA DTALNLKGLE DINLTELFNI SKEFWEKEVE EIKQYFEGQV
610 620
NADLPYEIER ELLALEMRIK QL
Length:622
Mass (Da):69,528
Last modified:August 13, 1987 - v1
Checksum:i1BB1E708A3D5E722
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F1NKP4F1NKP4_CHICK
Phosphoenolpyruvate carboxykinase, ...
PCK1
622Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14229 mRNA Translation: AAA49005.1
PIRiA26494 QYCHGC
RefSeqiNP_990802.1, NM_205471.1
UniGeneiGga.4447

Genome annotation databases

GeneIDi396458
KEGGigga:396458

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14229 mRNA Translation: AAA49005.1
PIRiA26494 QYCHGC
RefSeqiNP_990802.1, NM_205471.1
UniGeneiGga.4447

3D structure databases

ProteinModelPortaliP05153
SMRiP05153
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000012342

Proteomic databases

PaxDbiP05153
PRIDEiP05153

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi396458
KEGGigga:396458

Organism-specific databases

CTDi5105

Phylogenomic databases

eggNOGiKOG3749 Eukaryota
COG1274 LUCA
HOGENOMiHOG000191700
HOVERGENiHBG053651
InParanoidiP05153
KOiK01596
PhylomeDBiP05153

Enzyme and pathway databases

UniPathwayi
UPA00138

ReactomeiR-GGA-352875 Gluconeogenesis
SABIO-RKiP05153

Miscellaneous databases

PROiPR:P05153

Family and domain databases

CDDicd00819 PEPCK_GTP, 1 hit
Gene3Di3.40.449.10, 2 hits
3.90.228.20, 2 hits
HAMAPiMF_00452 PEPCK_GTP, 1 hit
InterProiView protein in InterPro
IPR018091 PEP_carboxykin_GTP_CS
IPR013035 PEP_carboxykinase_C
IPR008209 PEP_carboxykinase_GTP
IPR035077 PEP_carboxykinase_GTP_C
IPR035078 PEP_carboxykinase_GTP_N
IPR008210 PEP_carboxykinase_N
PANTHERiPTHR11561 PTHR11561, 1 hit
PfamiView protein in Pfam
PF00821 PEPCK_C, 1 hit
PF17297 PEPCK_N, 1 hit
PIRSFiPIRSF001348 PEP_carboxykinase_GTP, 1 hit
SUPFAMiSSF68923 SSF68923, 1 hit
PROSITEiView protein in PROSITE
PS00505 PEPCK_GTP, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPCKGC_CHICK
AccessioniPrimary (citable) accession number: P05153
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: September 12, 2018
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again