UniProtKB - P05150 (OTC_YEAST)
Protein
Ornithine carbamoyltransferase
Gene
ARG3
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Miscellaneous
Present with 1140 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- EC:2.1.3.3
Activity regulationi
Forms a stable complex with CAR1 in the presence of ornithine and arginine. In this complex CAR1 retains activity, but ARG3 activity is inhibited.1 Publication
: L-arginine biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.Proteins known to be involved in the 3 steps of the subpathway in this organism are:
- Ornithine carbamoyltransferase (ARG3)
- Argininosuccinate synthase (ARG1)
- Argininosuccinate lyase (ARG4)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 118 | Carbamoyl phosphateBy similarity | 1 | |
Binding sitei | 118 | OrnithineBy similarity | 1 | |
Binding sitei | 145 | Carbamoyl phosphateBy similarity | 1 | |
Binding sitei | 185 | OrnithineBy similarity | 1 | |
Active sitei | 289 | 1 | ||
Binding sitei | 316 | Carbamoyl phosphateBy similarity | 1 | |
Binding sitei | 316 | OrnithineBy similarity | 1 |
GO - Molecular functioni
- amino acid binding Source: InterPro
- ornithine carbamoyltransferase activity Source: SGD
GO - Biological processi
- arginine biosynthetic process Source: SGD
- arginine biosynthetic process via ornithine Source: GO_Central
- citrulline biosynthetic process Source: GO_Central
Keywordsi
Molecular function | Transferase |
Biological process | Amino-acid biosynthesis, Arginine biosynthesis |
Enzyme and pathway databases
BioCyci | MetaCyc:YJL088W-MONOMER |
Reactomei | R-SCE-1268020, Mitochondrial protein import R-SCE-70635, Urea cycle |
SABIO-RKi | P05150 |
UniPathwayi | UPA00068;UER00112 |
Names & Taxonomyi
Protein namesi | Recommended name: Ornithine carbamoyltransferase (EC:2.1.3.3)Alternative name(s): Ornithine transcarbamylase Short name: OTCase |
Gene namesi | Name:ARG3 Ordered Locus Names:YJL088W ORF Names:J0924 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000003624, ARG3 |
VEuPathDBi | FungiDB:YJL088W |
Subcellular locationi
Cytoplasm and Cytosol
Cytosol
- cytosol Source: SGD
Mitochondrion
- mitochondrion Source: GO_Central
Other locations
- intracellular membrane-bounded organelle Source: GO_Central
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 68 | T → G: Reduces activity by 95%. Reduces affinity for ornithine 2-fold. 1 Publication | 1 | |
Mutagenesisi | 181 | G → R: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 182 | D → N: Reduces activity by 33%. Reduces affinity for ornithine 30-fold. 1 Publication | 1 | |
Mutagenesisi | 184 | N → Q: Reduces activity by 50%. Reduces affinity for ornithine 20-fold. 1 Publication | 1 | |
Mutagenesisi | 185 | N → Q: No effect on activity. Reduces affinity for ornithine 200-fold. 1 Publication | 1 | |
Mutagenesisi | 256 | E → Q: Reduces activity by 50%. 1 Publication | 1 | |
Mutagenesisi | 263 | K → A: Reduces activity by 70%. Reduces affinity for ornithine 18-fold. 1 Publication | 1 | |
Mutagenesisi | 289 | C → S: Reduces activity by 90%. Reduces affinity for ornithine 6-fold. 1 Publication | 1 | |
Mutagenesisi | 290 | L → S: Reduces activity by 86%. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000113082 | 2 – 338 | Ornithine carbamoyltransferaseAdd BLAST | 337 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineCombined sources | 1 |
Keywords - PTMi
AcetylationProteomic databases
MaxQBi | P05150 |
PaxDbi | P05150 |
PRIDEi | P05150 |
PTM databases
iPTMneti | P05150 |
Interactioni
Subunit structurei
Interacts with CAR1.
1 PublicationBinary interactionsi
P05150
With | #Exp. | IntAct |
---|---|---|
CAR1 [P00812] | 4 | EBI-12712,EBI-2856 |
Protein-protein interaction databases
BioGRIDi | 33669, 27 interactors |
ComplexPortali | CPX-1707, Ornithine carbamoyltransferase arginase complex |
DIPi | DIP-8093N |
IntActi | P05150, 60 interactors |
MINTi | P05150 |
STRINGi | 4932.YJL088W |
Miscellaneous databases
RNActi | P05150, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 67 – 71 | Ornithine and carbamoyl phosphate bindingBy similarity | 5 | |
Regioni | 145 – 148 | Ornithine and carbamoyl phosphate bindingBy similarity | 4 | |
Regioni | 249 – 253 | Ornithine bindingBy similarity | 5 | |
Regioni | 288 – 291 | Ornithine bindingBy similarity | 4 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1504, Eukaryota |
GeneTreei | ENSGT00510000047417 |
HOGENOMi | CLU_043846_3_0_1 |
InParanoidi | P05150 |
OMAi | DGNNVCN |
Family and domain databases
Gene3Di | 3.40.50.1370, 2 hits |
InterProi | View protein in InterPro IPR006132, Asp/Orn_carbamoyltranf_P-bd IPR006130, Asp/Orn_carbamoylTrfase IPR036901, Asp/Orn_carbamoylTrfase_sf IPR006131, Asp_carbamoyltransf_Asp/Orn-bd IPR002292, Orn/put_carbamltrans |
Pfami | View protein in Pfam PF00185, OTCace, 1 hit PF02729, OTCace_N, 1 hit |
PRINTSi | PR00100, AOTCASE PR00102, OTCASE |
SUPFAMi | SSF53671, SSF53671, 1 hit |
TIGRFAMsi | TIGR00658, orni_carb_tr, 1 hit |
PROSITEi | View protein in PROSITE PS00097, CARBAMOYLTRANSFERASE, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P05150-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSTTASTPSS LRHLISIKDL SDEEFRILVQ RAQHFKNVFK ANKTNDFQSN
60 70 80 90 100
HLKLLGRTIA LIFTKRSTRT RISTEGAATF FGAQPMFLGK EDIQLGVNES
110 120 130 140 150
FYDTTKVVSS MVSCIFARVN KHEDILAFCK DSSVPIINSL CDKFHPLQAI
160 170 180 190 200
CDLLTIIENF NISLDEVNKG INSKLKMAWI GDANNVINDM CIACLKFGIS
210 220 230 240 250
VSISTPPGIE MDSDIVDEAK KVAERNGATF ELTHDSLKAS TNANILVTDT
260 270 280 290 300
FVSMGEEFAK QAKLKQFKGF QINQELVSVA DPNYKFMHCL PRHQEEVSDD
310 320 330
VFYGEHSIVF EEAENRLYAA MSAIDIFVNN KGNFKDLK
Sequence cautioni
The sequence CAA58480 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA89381 differs from that shown. Reason: Erroneous initiation.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M11946 Genomic DNA Translation: AAA34432.1 X83502 Genomic DNA Translation: CAA58480.1 Different initiation. Z49363 Genomic DNA Translation: CAA89381.1 Different initiation. M28301 Genomic DNA Translation: AAA34433.1 BK006943 Genomic DNA Translation: DAA08712.1 |
PIRi | S56020, OWBY |
RefSeqi | NP_012447.1, NM_001181521.1 |
Genome annotation databases
EnsemblFungii | YJL088W_mRNA; YJL088W; YJL088W |
GeneIDi | 853357 |
KEGGi | sce:YJL088W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M11946 Genomic DNA Translation: AAA34432.1 X83502 Genomic DNA Translation: CAA58480.1 Different initiation. Z49363 Genomic DNA Translation: CAA89381.1 Different initiation. M28301 Genomic DNA Translation: AAA34433.1 BK006943 Genomic DNA Translation: DAA08712.1 |
PIRi | S56020, OWBY |
RefSeqi | NP_012447.1, NM_001181521.1 |
3D structure databases
SMRi | P05150 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 33669, 27 interactors |
ComplexPortali | CPX-1707, Ornithine carbamoyltransferase arginase complex |
DIPi | DIP-8093N |
IntActi | P05150, 60 interactors |
MINTi | P05150 |
STRINGi | 4932.YJL088W |
PTM databases
iPTMneti | P05150 |
Proteomic databases
MaxQBi | P05150 |
PaxDbi | P05150 |
PRIDEi | P05150 |
Genome annotation databases
EnsemblFungii | YJL088W_mRNA; YJL088W; YJL088W |
GeneIDi | 853357 |
KEGGi | sce:YJL088W |
Organism-specific databases
SGDi | S000003624, ARG3 |
VEuPathDBi | FungiDB:YJL088W |
Phylogenomic databases
eggNOGi | KOG1504, Eukaryota |
GeneTreei | ENSGT00510000047417 |
HOGENOMi | CLU_043846_3_0_1 |
InParanoidi | P05150 |
OMAi | DGNNVCN |
Enzyme and pathway databases
UniPathwayi | UPA00068;UER00112 |
BioCyci | MetaCyc:YJL088W-MONOMER |
Reactomei | R-SCE-1268020, Mitochondrial protein import R-SCE-70635, Urea cycle |
SABIO-RKi | P05150 |
Miscellaneous databases
PROi | PR:P05150 |
RNActi | P05150, protein |
Family and domain databases
Gene3Di | 3.40.50.1370, 2 hits |
InterProi | View protein in InterPro IPR006132, Asp/Orn_carbamoyltranf_P-bd IPR006130, Asp/Orn_carbamoylTrfase IPR036901, Asp/Orn_carbamoylTrfase_sf IPR006131, Asp_carbamoyltransf_Asp/Orn-bd IPR002292, Orn/put_carbamltrans |
Pfami | View protein in Pfam PF00185, OTCace, 1 hit PF02729, OTCace_N, 1 hit |
PRINTSi | PR00100, AOTCASE PR00102, OTCASE |
SUPFAMi | SSF53671, SSF53671, 1 hit |
TIGRFAMsi | TIGR00658, orni_carb_tr, 1 hit |
PROSITEi | View protein in PROSITE PS00097, CARBAMOYLTRANSFERASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | OTC_YEAST | |
Accessioni | P05150Primary (citable) accession number: P05150 Secondary accession number(s): D6VW96 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 13, 1987 |
Last sequence update: | August 13, 1987 | |
Last modified: | April 7, 2021 | |
This is version 182 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome X
Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families