UniProtKB - P05131 (KAPCB_BOVIN)
cAMP-dependent protein kinase catalytic subunit beta
PRKACB
Functioni
Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, and differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates GPKOW which regulates its ability to bind RNA.
By similarityCatalytic activityi
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 73 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 167 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 50 – 58 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- AMP-activated protein kinase activity Source: UniProtKB-EC
- ATP binding Source: UniProtKB-KW
- cAMP-dependent protein kinase activity Source: UniProtKB-EC
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein serine kinase activity Source: RHEA
GO - Biological processi
- protein kinase A signaling Source: GO_Central
- protein phosphorylation Source: UniProtKB
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Ligand | ATP-binding, cAMP, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.11.11, 908 |
Names & Taxonomyi
Protein namesi | Recommended name: cAMP-dependent protein kinase catalytic subunit beta (EC:2.7.11.11)Short name: PKA C-beta |
Gene namesi | Name:PRKACB |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Plasma membrane
- Cell membrane By similarity
Other locations
- Membrane By similarity; Lipid-anchor By similarity
Note: Translocates into the nucleus (monomeric catalytic subunit). The inactive holoenzyme is found in the cytoplasm.
Nucleus
- nucleus Source: GO_Central
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Membrane, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000086057 | 2 – 351 | cAMP-dependent protein kinase catalytic subunit betaAdd BLAST | 350 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 2 | N-myristoyl glycine1 Publication | 1 | |
Modified residuei | 3 | Deamidated asparagine; partial3 Publications | 1 | |
Modified residuei | 11 | PhosphoserineBy similarity | 1 | |
Modified residuei | 69 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 140 | PhosphoserineBy similarity | 1 | |
Modified residuei | 198 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 322 | PhosphoserineBy similarity | 1 | |
Modified residuei | 331 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 339 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Lipoprotein, Myristate, PhosphoproteinProteomic databases
PaxDbi | P05131 |
PRIDEi | P05131 |
PTM databases
iPTMneti | P05131 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSBTAG00000011953, Expressed in occipital lobe and 96 other tissues |
Interactioni
Subunit structurei
A number of inactive tetrameric holoenzymes are produced by the combination of homo- or heterodimers of the different regulatory subunits associated with two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits.
Interacts with PRKAR1A and PRKAR2B (By similarity). The cAMP-dependent protein kinase catalytic subunit binds PJA2.
Interacts with GPKOW.
By similarityProtein-protein interaction databases
BioGRIDi | 159585, 27 interactors |
IntActi | P05131, 27 interactors |
STRINGi | 9913.ENSBTAP00000046803 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 44 – 298 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 255 | |
Domaini | 299 – 351 | AGC-kinase C-terminalPROSITE-ProRule annotationAdd BLAST | 53 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0616, Eukaryota |
HOGENOMi | CLU_000288_63_5_1 |
InParanoidi | P05131 |
OrthoDBi | 963519at2759 |
TreeFami | TF313399 |
Family and domain databases
CDDi | cd14209, STKc_PKA, 1 hit |
InterProi | View protein in InterPro IPR000961, AGC-kinase_C IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS IPR044109, STKc_PKA |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00133, S_TK_X, 1 hit SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS51285, AGC_KINASE_CTER, 1 hit PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGNAATAKKG SEVESVKEFL AKAKEDFLKK WENPAPNNAG LEDFERKKTL
60 70 80 90 100
GTGSFGRVML VKHKATEQYY AMKILDKQKV VKLKQIEHTL NEKRILQAVN
110 120 130 140 150
FPFLVRLEYA FKDNSNLYMV MEYVPGGEMF SHLRRIGRFS EPHARFYAAQ
160 170 180 190 200
IVLTFEYLHS LDLIYRDLKP ENLLIDHQGY IQVTDFGFAK RVKGRTWTLC
210 220 230 240 250
GTPEYLAPEI ILSKGYNKAV DWWALGVLIY EMAAGYPPFF ADQPIQIYEK
260 270 280 290 300
IVSGKVRFPS HFSSDLKDLL RNLLQVDLTK RFGNLKNGVS DIKTHKWFAT
310 320 330 340 350
TDWIAIYQRK VEAPFIPKFR GSGDTSNFDD YEEEDIRVSI TEKCGKEFCE
F
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_031246 | 1 – 16 | MGNAA…EVESV → MAAYREVPCNQYTGTTALQK LEGFASRLFHRHSKGTAHDQ KTALENDSLHFSEHTALWDR SM in isoform 2. 1 PublicationAdd BLAST | 16 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J02647 mRNA Translation: AAA30707.1 M60482 mRNA Translation: AAA30424.1 |
PIRi | A23716, OKBOB2 A25334, OKBOB1 |
RefSeqi | NP_777010.1, NM_174585.3 [P05131-1] XP_005204430.1, XM_005204373.3 |
Genome annotation databases
GeneIDi | 282323 |
KEGGi | bta:282323 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J02647 mRNA Translation: AAA30707.1 M60482 mRNA Translation: AAA30424.1 |
PIRi | A23716, OKBOB2 A25334, OKBOB1 |
RefSeqi | NP_777010.1, NM_174585.3 [P05131-1] XP_005204430.1, XM_005204373.3 |
3D structure databases
SMRi | P05131 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 159585, 27 interactors |
IntActi | P05131, 27 interactors |
STRINGi | 9913.ENSBTAP00000046803 |
Chemistry databases
ChEMBLi | CHEMBL2111446 |
DrugCentrali | P05131 |
PTM databases
iPTMneti | P05131 |
Proteomic databases
PaxDbi | P05131 |
PRIDEi | P05131 |
Genome annotation databases
GeneIDi | 282323 |
KEGGi | bta:282323 |
Organism-specific databases
CTDi | 5567 |
Phylogenomic databases
eggNOGi | KOG0616, Eukaryota |
HOGENOMi | CLU_000288_63_5_1 |
InParanoidi | P05131 |
OrthoDBi | 963519at2759 |
TreeFami | TF313399 |
Enzyme and pathway databases
BRENDAi | 2.7.11.11, 908 |
Gene expression databases
Bgeei | ENSBTAG00000011953, Expressed in occipital lobe and 96 other tissues |
Family and domain databases
CDDi | cd14209, STKc_PKA, 1 hit |
InterProi | View protein in InterPro IPR000961, AGC-kinase_C IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS IPR044109, STKc_PKA |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00133, S_TK_X, 1 hit SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS51285, AGC_KINASE_CTER, 1 hit PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | KAPCB_BOVIN | |
Accessioni | P05131Primary (citable) accession number: P05131 Secondary accession number(s): P24256 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 13, 1987 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 192 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families