Nppa

Functionⁱ

Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.3 Publications

GO - Molecular functionⁱ

hormone activity Source: MGI
hormone receptor binding Source: MGI
neuropeptide hormone activity Source: MGI
neuropeptide receptor binding Source: MGI
signaling receptor binding Source: MGI

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cardiac muscle hypertrophy in response to stress
Source: MGI
Inferred from direct assayⁱ
- 24137001
cell growth involved in cardiac muscle cell development Source: Ensembl
cellular response to mechanical stimulus Source: Ensembl
cGMP biosynthetic process Source: UniProtKB
cGMP-mediated signaling Source: MGI
female pregnancy
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 22437503
negative regulation of cell growth Source: MGI
negative regulation of collecting lymphatic vessel constriction Source: MGI
negative regulation of systemic arterial blood pressure Source: MGI
neuropeptide signaling pathway Source: MGI
positive regulation of cardiac muscle contraction Source: MGI
positive regulation of cGMP-mediated signaling Source: MGI
positive regulation of delayed rectifier potassium channel activity Source: MGI
positive regulation of heart rate Source: MGI
positive regulation of histamine secretion by mast cell Source: MGI
positive regulation of potassium ion export across plasma membrane Source: MGI
protein folding Source: MGI
receptor guanylyl cyclase signaling pathway Source: UniProtKB
regulation of atrial cardiac muscle cell membrane repolarization Source: MGI
regulation of blood pressure
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 22437503
regulation of blood vessel size Source: UniProtKB-KW
regulation of calcium ion transmembrane transport via high voltage-gated calcium channel Source: MGI
regulation of high voltage-gated calcium channel activity Source: MGI
response to hypoxia Source: Ensembl
response to insulin Source: Ensembl

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Hormone, Vasoactive

Molecular function

Hormone, Vasoactive

Enzyme and pathway databases

Reactomeⁱ	R-MMU-5578768 Physiological factors

Reactomeⁱ

R-MMU-5578768 Physiological factors

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Natriuretic peptides A Alternative name(s): Prepronatriodilatin Cleaved into the following 5 chains: Atrial natriuretic factor Short name: ANF Alternative name(s): Atrial natriuretic peptide Short name: ANP Auriculin-B Auriculin-A Atriopeptin-1 Alternative name(s): Atriopeptin I Atriopeptin-2 Alternative name(s): Atriopeptin II
Gene namesⁱ	Name:Nppa Synonyms:Pnd
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 4

Organism-specific databases

MGIⁱ	MGI:97367 Nppa

Keywords - Cellular componentⁱ

Secreted

Pathology & Biotechⁱ

Disruption phenotypeⁱ

Mice display increased arterial blood pressure which is dietary salt intake independent. During pregnancy, increased blood pressure is observed, leading to late gestational proteinuria and smaller litters. Impaired trophoblast invasion and smaller spiral arteries are also observed in E12.5 embryos. In E18.5 embryos, mice display fewer trophoblasts and smaller arteries in the decidua and myometrium than those in wild-type mice.2 Publications

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	122	R → A: Abolishes maturation and cleavage by CORIN. 1 Publication	1
Mutagenesisⁱ	125	R → A: Does not affect maturation and cleavage by CORIN. 1 Publication	1
Mutagenesisⁱ	126	R → A: Does not affect maturation and cleavage by CORIN. 1 Publication	1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Length
Signal peptideⁱ	1 – 24	By similarityAdd BLAST	24
PropeptideⁱPRO_0000001495	25 – 122	CuratedAdd BLAST	98
PeptideⁱPRO_0000391784	123 – 150	Atrial natriuretic factor1 PublicationAdd BLAST	28
PeptideⁱPRO_0000001496	126 – 150	Auriculin-BBy similarityAdd BLAST	25
PeptideⁱPRO_0000001497	126 – 149	Auriculin-ABy similarityAdd BLAST	24
PeptideⁱPRO_0000001498	127 – 149	Atriopeptin-1By similarityAdd BLAST	23
PeptideⁱPRO_0000001499	127 – 147	Atriopeptin-2By similarityAdd BLAST	21

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Disulfide bondⁱ	129 ↔ 145	By similarity

Post-translational modificationⁱ

Cleaved by CORIN upon secretion to produce the functional hormone.2 Publications

Atrial natriuretic factor: Cleaved by MME. The cleavage initiates degradation of the factor and thereby regulate its activity.By similarity

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Siteⁱ	122 – 123	Cleavage; by CORIN1 Publication		2
Siteⁱ	129 – 130	Cleavage; by MMEBy similarity		2

Keywords - PTMⁱ

Cleavage on pair of basic residues, Disulfide bond

Proteomic databases

PaxDbⁱ	P05125
PeptideAtlas More...PeptideAtlasⁱ	P05125
PRIDEⁱ	P05125

PTM databases

iPTMnetⁱ	P05125
PhosphoSitePlusⁱ	P05125

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSMUSG00000041616 Expressed in 83 organ(s), highest expression level in cardiac atrium
CleanExⁱ	MM_NPPA
Genevisibleⁱ	P05125 MM

Interactionⁱ

GO - Molecular functionⁱ

hormone activity Source: MGI
hormone receptor binding Source: MGI
neuropeptide hormone activity Source: MGI
neuropeptide receptor binding Source: MGI
signaling receptor binding Source: MGI

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

IntActⁱ	P05125, 4 interactors
Molecular INTeraction database More...MINTⁱ	P05125
STRINGⁱ	10090.ENSMUSP00000099520

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P05125
SMRⁱ	P05125
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the natriuretic peptide family.Curated

Keywords - Domainⁱ

Signal

Phylogenomic databases

eggNOGⁱ	ENOG410IW5F Eukaryota ENOG410ZUY6 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00510000048659
HOGENOMⁱ	HOG000033937
HOVERGENⁱ	HBG004227
InParanoidⁱ	P05125
KEGG Orthology (KO) More...KOⁱ	K12334
OMAⁱ	CNSFRYR
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0SV3
TreeFamⁱ	TF106304

Family and domain databases

InterProⁱ	View protein in InterPro IPR000663 Natr_peptide IPR030480 Natr_peptide_CS IPR002407 Natriuretic_peptide_atrial
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00212 ANP, 1 hit
PRINTSⁱ	PR00711 ANATPEPTIDE PR00710 NATPEPTIDES
SMARTⁱ	View protein in SMART SM00183 NAT_PEP, 1 hit
PROSITEⁱ	View protein in PROSITE PS00263 NATRIURETIC_PEPTIDE, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

P05125-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MGSFSITLGF FLVLAFWLPG HIGANPVYSA VSNTDLMDFK NLLDHLEEKM 
        60         70         80         90        100
PVEDEVMPPQ ALSEQTEEAG AALSSLPEVP PWTGEVNPPL RDGSALGRSP 
       110        120        130        140        150
WDPSDRSALL KSKLRALLAG PRSLRRSSCF GGRIDRIGAQ SGLGCNSFRY 

RR

Length:152

Mass (Da):16,572

Last modified:October 3, 2012 - v2

Checksum:ⁱ1693D134B5FCA23D

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	96 – 97	LG → SR in AAA37235 (PubMed:6542248).Curated		2

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	K02781 Genomic DNA Translation: AAA37235.1 AK147180 mRNA Translation: BAE27742.1 AL714013 Genomic DNA Translation: CAM14483.1 CU210867 Genomic DNA Translation: CAQ52160.1 CH466594 Genomic DNA Translation: EDL14792.1 BC089615 mRNA Translation: AAH89615.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS18927.1
PIRⁱ	A29370 AWMS
NCBI Reference Sequences More...RefSeqⁱ	NP_032751.1, NM_008725.3
UniGeneⁱ	Mm.482123

Genome annotation databases

Ensemblⁱ	ENSMUST00000103230; ENSMUSP00000099520; ENSMUSG00000041616
GeneIDⁱ	230899
KEGGⁱ	mmu:230899
UCSC genome browser More...UCSCⁱ	uc008vtq.1 mouse

Similar proteinsⁱ

50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P05125	Natriuretic peptides A	)	HUMAN		153	UniRef50_P01160
Natriuretic peptides A		HORSE		153
Natriuretic peptides A	)	RAT		152
Natriuretic peptides A		RABIT		153
Natriuretic peptides A (Fragment)		CAVPO		128
+86

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	K02781 Genomic DNA Translation: AAA37235.1 AK147180 mRNA Translation: BAE27742.1 AL714013 Genomic DNA Translation: CAM14483.1 CU210867 Genomic DNA Translation: CAQ52160.1 CH466594 Genomic DNA Translation: EDL14792.1 BC089615 mRNA Translation: AAH89615.1
CCDSⁱ	CCDS18927.1
PIRⁱ	A29370 AWMS
RefSeqⁱ	NP_032751.1, NM_008725.3
UniGeneⁱ	Mm.482123

3D structure databases

ProteinModelPortalⁱ	P05125
SMRⁱ	P05125
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

IntActⁱ	P05125, 4 interactors
MINTⁱ	P05125
STRINGⁱ	10090.ENSMUSP00000099520

PTM databases

iPTMnetⁱ	P05125
PhosphoSitePlusⁱ	P05125

Proteomic databases

PaxDbⁱ	P05125
PeptideAtlasⁱ	P05125
PRIDEⁱ	P05125

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000103230; ENSMUSP00000099520; ENSMUSG00000041616
GeneIDⁱ	230899
KEGGⁱ	mmu:230899
UCSCⁱ	uc008vtq.1 mouse

Organism-specific databases

CTDⁱ	4878
MGIⁱ	MGI:97367 Nppa

Phylogenomic databases

eggNOGⁱ	ENOG410IW5F Eukaryota ENOG410ZUY6 LUCA
GeneTreeⁱ	ENSGT00510000048659
HOGENOMⁱ	HOG000033937
HOVERGENⁱ	HBG004227
InParanoidⁱ	P05125
KOⁱ	K12334
OMAⁱ	CNSFRYR
OrthoDBⁱ	EOG091G0SV3
TreeFamⁱ	TF106304

Enzyme and pathway databases

Reactomeⁱ	R-MMU-5578768 Physiological factors

Reactomeⁱ

R-MMU-5578768 Physiological factors

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P05125
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000041616 Expressed in 83 organ(s), highest expression level in cardiac atrium
CleanExⁱ	MM_NPPA
Genevisibleⁱ	P05125 MM

Family and domain databases

InterProⁱ	View protein in InterPro IPR000663 Natr_peptide IPR030480 Natr_peptide_CS IPR002407 Natriuretic_peptide_atrial
Pfamⁱ	View protein in Pfam PF00212 ANP, 1 hit
PRINTSⁱ	PR00711 ANATPEPTIDE PR00710 NATPEPTIDES
SMARTⁱ	View protein in SMART SM00183 NAT_PEP, 1 hit
PROSITEⁱ	View protein in PROSITE PS00263 NATRIURETIC_PEPTIDE, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	ANF_MOUSE
Accessionⁱ	P05125Primary (citable) accession number: P05125 Secondary accession number(s): Q5FW59
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
	Last sequence update:	October 3, 2012
	Last modified:	November 7, 2018
	This is version 145 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P05125 (ANF_MOUSE)

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Natriuretic peptides A

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted

Extracellular region or secreted

Lysosome

Nucleus

Other locations

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Sites

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ