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Protein

DNA-3-methyladenine glycosylase 1

Gene

tag

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine from the damaged DNA polymer formed by alkylation lesions.1 Publication

Catalytic activityi

Hydrolysis of alkylated DNA, releasing 3-methyladenine.1 Publication

Activity regulationi

Activity is controlled by product inhibition.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi4ZincCombined sources2 Publications1
Metal bindingi17Zinc; via tele nitrogenCombined sources2 Publications1
Metal bindingi175Zinc; via pros nitrogenCombined sources2 Publications1
Metal bindingi179ZincCombined sources2 Publications1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processDNA damage, DNA repair
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:EG10986-MONOMER
MetaCyc:EG10986-MONOMER
BRENDAi3.2.2.20 2026

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-3-methyladenine glycosylase 1 (EC:3.2.2.201 Publication)
Alternative name(s):
3-methyladenine-DNA glycosylase I, constitutive
Short name:
TAG I
DNA-3-methyladenine glycosidase I
DNA-3-methyladenine glycosylase I
Gene namesi
Name:tag2 Publications
Ordered Locus Names:b3549, JW3518
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10986 tag

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2295563
DrugBankiDB04104 3-Methyladenine

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001948771 – 187DNA-3-methyladenine glycosylase 1Add BLAST187

Proteomic databases

EPDiP05100
PaxDbiP05100
PRIDEiP05100

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
aceEP0AFG82EBI-558722,EBI-542683

Protein-protein interaction databases

BioGridi4261555, 196 interactors
DIPiDIP-10950N
IntActiP05100, 5 interactors
STRINGi316385.ECDH10B_3728

Structurei

Secondary structure

1187
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP05100
SMRiP05100
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP05100

Family & Domainsi

Phylogenomic databases

eggNOGiENOG4108R3E Bacteria
COG2818 LUCA
HOGENOMiHOG000220753
InParanoidiP05100
KOiK01246
PhylomeDBiP05100

Family and domain databases

InterProiView protein in InterPro
IPR005019 Adenine_glyco
IPR011257 DNA_glycosylase
IPR004597 Tag
PfamiView protein in Pfam
PF03352 Adenine_glyco, 1 hit
SUPFAMiSSF48150 SSF48150, 1 hit
TIGRFAMsiTIGR00624 tag, 1 hit

Sequencei

Sequence statusi: Complete.

P05100-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MERCGWVSQD PLYIAYHDNE WGVPETDSKK LFEMICLEGQ QAGLSWITVL
60 70 80 90 100
KKRENYRACF HQFDPVKVAA MQEEDVERLV QDAGIIRHRG KIQAIIGNAR
110 120 130 140 150
AYLQMEQNGE PFVDFVWSFV NHQPQVTQAT TLSEIPTSTS ASDALSKALK
160 170 180
KRGFKFVGTT ICYSFMQACG LVNDHVVGCC CYPGNKP
Length:187
Mass (Da):21,100
Last modified:August 13, 1987 - v1
Checksum:i5A305B5CA66A48FE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02606 Genomic DNA Translation: AAA24658.1
X03845 Genomic DNA Translation: CAA27472.1
U00039 Genomic DNA Translation: AAB18526.1
U00096 Genomic DNA Translation: AAC76573.1
AP009048 Genomic DNA Translation: BAE77746.1
PIRiA24604 DGECM1
RefSeqiNP_418005.1, NC_000913.3
WP_000438957.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76573; AAC76573; b3549
BAE77746; BAE77746; BAE77746
GeneIDi947137
KEGGiecj:JW3518
eco:b3549
PATRICifig|1411691.4.peg.3165

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02606 Genomic DNA Translation: AAA24658.1
X03845 Genomic DNA Translation: CAA27472.1
U00039 Genomic DNA Translation: AAB18526.1
U00096 Genomic DNA Translation: AAC76573.1
AP009048 Genomic DNA Translation: BAE77746.1
PIRiA24604 DGECM1
RefSeqiNP_418005.1, NC_000913.3
WP_000438957.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1LMZNMR-A1-187[»]
1NKUNMR-A1-187[»]
1P7MNMR-A1-187[»]
ProteinModelPortaliP05100
SMRiP05100
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261555, 196 interactors
DIPiDIP-10950N
IntActiP05100, 5 interactors
STRINGi316385.ECDH10B_3728

Chemistry databases

ChEMBLiCHEMBL2295563
DrugBankiDB04104 3-Methyladenine

Proteomic databases

EPDiP05100
PaxDbiP05100
PRIDEiP05100

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76573; AAC76573; b3549
BAE77746; BAE77746; BAE77746
GeneIDi947137
KEGGiecj:JW3518
eco:b3549
PATRICifig|1411691.4.peg.3165

Organism-specific databases

EchoBASEiEB0979
EcoGeneiEG10986 tag

Phylogenomic databases

eggNOGiENOG4108R3E Bacteria
COG2818 LUCA
HOGENOMiHOG000220753
InParanoidiP05100
KOiK01246
PhylomeDBiP05100

Enzyme and pathway databases

BioCyciEcoCyc:EG10986-MONOMER
MetaCyc:EG10986-MONOMER
BRENDAi3.2.2.20 2026

Miscellaneous databases

EvolutionaryTraceiP05100
PROiPR:P05100

Family and domain databases

InterProiView protein in InterPro
IPR005019 Adenine_glyco
IPR011257 DNA_glycosylase
IPR004597 Tag
PfamiView protein in Pfam
PF03352 Adenine_glyco, 1 hit
SUPFAMiSSF48150 SSF48150, 1 hit
TIGRFAMsiTIGR00624 tag, 1 hit
ProtoNetiSearch...

Entry informationi

Entry namei3MG1_ECOLI
AccessioniPrimary (citable) accession number: P05100
Secondary accession number(s): Q2M7L0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: November 7, 2018
This is version 154 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
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Main funding by: National Institutes of Health

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