UniProtKB - P05100 (3MG1_ECOLI)
Protein
DNA-3-methyladenine glycosylase 1
Gene
tag
Organism
Escherichia coli (strain K12)
Status
Functioni
Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine from the damaged DNA polymer formed by alkylation lesions.1 Publication
Catalytic activityi
- Hydrolysis of alkylated DNA, releasing 3-methyladenine.1 Publication EC:3.2.2.20
Activity regulationi
Activity is controlled by product inhibition.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 4 | ZincCombined sources2 Publications | 1 | |
Metal bindingi | 17 | Zinc; via tele nitrogenCombined sources2 Publications | 1 | |
Metal bindingi | 175 | Zinc; via pros nitrogenCombined sources2 Publications | 1 | |
Metal bindingi | 179 | ZincCombined sources2 Publications | 1 |
GO - Molecular functioni
- DNA-3-methyladenine glycosylase activity Source: GO_Central
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- base-excision repair Source: InterPro
- DNA dealkylation involved in DNA repair Source: GO_Central
Keywordsi
Molecular function | Hydrolase |
Biological process | DNA damage, DNA repair |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10986-MONOMER MetaCyc:EG10986-MONOMER |
BRENDAi | 3.2.2.20, 2026 |
Names & Taxonomyi
Protein namesi | Recommended name: DNA-3-methyladenine glycosylase 1 (EC:3.2.2.201 Publication)Alternative name(s): 3-methyladenine-DNA glycosylase I, constitutive Short name: TAG I DNA-3-methyladenine glycosidase I DNA-3-methyladenine glycosylase I |
Gene namesi | Name:tag2 Publications Ordered Locus Names:b3549, JW3518 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000194877 | 1 – 187 | DNA-3-methyladenine glycosylase 1Add BLAST | 187 |
Proteomic databases
jPOSTi | P05100 |
PaxDbi | P05100 |
PRIDEi | P05100 |
Interactioni
Binary interactionsi
P05100
With | #Exp. | IntAct |
---|---|---|
aceE [P0AFG8] | 2 | EBI-558722,EBI-542683 |
Protein-protein interaction databases
BioGRIDi | 4261555, 196 interactors 851471, 1 interactor |
DIPi | DIP-10950N |
IntActi | P05100, 5 interactors |
STRINGi | 511145.b3549 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P05100 |
SMRi | P05100 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P05100 |
Family & Domainsi
Phylogenomic databases
eggNOGi | COG2818, Bacteria |
HOGENOMi | CLU_083758_1_0_6 |
InParanoidi | P05100 |
PhylomeDBi | P05100 |
Family and domain databases
InterProi | View protein in InterPro IPR005019, Adenine_glyco IPR011257, DNA_glycosylase IPR004597, Tag |
Pfami | View protein in Pfam PF03352, Adenine_glyco, 1 hit |
SUPFAMi | SSF48150, SSF48150, 1 hit |
TIGRFAMsi | TIGR00624, tag, 1 hit |
i Sequence
Sequence statusi: Complete.
P05100-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MERCGWVSQD PLYIAYHDNE WGVPETDSKK LFEMICLEGQ QAGLSWITVL
60 70 80 90 100
KKRENYRACF HQFDPVKVAA MQEEDVERLV QDAGIIRHRG KIQAIIGNAR
110 120 130 140 150
AYLQMEQNGE PFVDFVWSFV NHQPQVTQAT TLSEIPTSTS ASDALSKALK
160 170 180
KRGFKFVGTT ICYSFMQACG LVNDHVVGCC CYPGNKP
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J02606 Genomic DNA Translation: AAA24658.1 X03845 Genomic DNA Translation: CAA27472.1 U00039 Genomic DNA Translation: AAB18526.1 U00096 Genomic DNA Translation: AAC76573.1 AP009048 Genomic DNA Translation: BAE77746.1 |
PIRi | A24604, DGECM1 |
RefSeqi | NP_418005.1, NC_000913.3 WP_000438957.1, NZ_SSZK01000068.1 |
Genome annotation databases
EnsemblBacteriai | AAC76573; AAC76573; b3549 BAE77746; BAE77746; BAE77746 |
GeneIDi | 947137 |
KEGGi | ecj:JW3518 eco:b3549 |
PATRICi | fig|1411691.4.peg.3165 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J02606 Genomic DNA Translation: AAA24658.1 X03845 Genomic DNA Translation: CAA27472.1 U00039 Genomic DNA Translation: AAB18526.1 U00096 Genomic DNA Translation: AAC76573.1 AP009048 Genomic DNA Translation: BAE77746.1 |
PIRi | A24604, DGECM1 |
RefSeqi | NP_418005.1, NC_000913.3 WP_000438957.1, NZ_SSZK01000068.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1LMZ | NMR | - | A | 1-187 | [»] | |
1NKU | NMR | - | A | 1-187 | [»] | |
1P7M | NMR | - | A | 1-187 | [»] | |
BMRBi | P05100 | |||||
SMRi | P05100 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261555, 196 interactors 851471, 1 interactor |
DIPi | DIP-10950N |
IntActi | P05100, 5 interactors |
STRINGi | 511145.b3549 |
Chemistry databases
ChEMBLi | CHEMBL2295563 |
DrugBanki | DB04104, 3-Methyladenine |
Proteomic databases
jPOSTi | P05100 |
PaxDbi | P05100 |
PRIDEi | P05100 |
Genome annotation databases
EnsemblBacteriai | AAC76573; AAC76573; b3549 BAE77746; BAE77746; BAE77746 |
GeneIDi | 947137 |
KEGGi | ecj:JW3518 eco:b3549 |
PATRICi | fig|1411691.4.peg.3165 |
Organism-specific databases
EchoBASEi | EB0979 |
Phylogenomic databases
eggNOGi | COG2818, Bacteria |
HOGENOMi | CLU_083758_1_0_6 |
InParanoidi | P05100 |
PhylomeDBi | P05100 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10986-MONOMER MetaCyc:EG10986-MONOMER |
BRENDAi | 3.2.2.20, 2026 |
Miscellaneous databases
EvolutionaryTracei | P05100 |
PROi | PR:P05100 |
Family and domain databases
InterProi | View protein in InterPro IPR005019, Adenine_glyco IPR011257, DNA_glycosylase IPR004597, Tag |
Pfami | View protein in Pfam PF03352, Adenine_glyco, 1 hit |
SUPFAMi | SSF48150, SSF48150, 1 hit |
TIGRFAMsi | TIGR00624, tag, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | 3MG1_ECOLI | |
Accessioni | P05100Primary (citable) accession number: P05100 Secondary accession number(s): Q2M7L0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 13, 1987 |
Last sequence update: | August 13, 1987 | |
Last modified: | December 2, 2020 | |
This is version 168 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references