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Protein

Fructose-bisphosphate aldolase A

Gene

Aldoa

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein (By similarity).By similarity

Miscellaneous

In vertebrates, three forms of this ubiquitous glycolytic enzyme are found, aldolase A in muscle, aldolase B in liver and aldolase C in brain.

Catalytic activityi

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (Gpi)
  3. ATP-dependent 6-phosphofructokinase (Pfkm), ATP-dependent 6-phosphofructokinase (Pfkp), ATP-dependent 6-phosphofructokinase (Pfkp), ATP-dependent 6-phosphofructokinase (Pfkm), ATP-dependent 6-phosphofructokinase, platelet type (Pfkp), ATP-dependent 6-phosphofructokinase, muscle type (Pfkm), ATP-dependent 6-phosphofructokinase, liver type (Pfkl), ATP-dependent 6-phosphofructokinase (Pfkl)
  4. Fructose-bisphosphate aldolase (Aldoart2), Fructose-bisphosphate aldolase (Aldob), Fructose-bisphosphate aldolase (Aldoc), Fructose-bisphosphate aldolase B (Aldob), Fructose-bisphosphate aldolase C (Aldoc), Fructose-bisphosphate aldolase A (Aldoa)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei56Substrate1
Binding sitei147Substrate1
Active sitei188Proton acceptorBy similarity1
Active sitei230Schiff-base intermediate with dihydroxyacetone-P1
Sitei364Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphate1

GO - Molecular functioni

GO - Biological processi

  • glycolytic process Source: UniProtKB
  • methylglyoxal biosynthetic process Source: RGD
  • protein homotetramerization Source: UniProtKB
  • response to estrogen Source: RGD
  • response to heat Source: RGD
  • response to hypoxia Source: RGD
  • response to lipopolysaccharide Source: RGD
  • response to nicotine Source: RGD

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandSchiff base

Enzyme and pathway databases

ReactomeiR-RNO-114608 Platelet degranulation
R-RNO-6798695 Neutrophil degranulation
R-RNO-70171 Glycolysis
R-RNO-70263 Gluconeogenesis
SABIO-RKiP05065
UniPathwayi
UPA00109;UER00183

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-bisphosphate aldolase A (EC:4.1.2.13)
Alternative name(s):
Muscle-type aldolase
Gene namesi
Name:Aldoa
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi2089 Aldoa

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002169392 – 364Fructose-bisphosphate aldolase AAdd BLAST363

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei5PhosphotyrosineCombined sources1
Modified residuei9PhosphothreonineCombined sources1
Modified residuei36PhosphoserineBy similarity1
Modified residuei39PhosphoserineBy similarity1
Modified residuei42N6-acetyllysine; alternateBy similarity1
Cross-linki42Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity
Cross-linki42Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei46PhosphoserineBy similarity1
Modified residuei108N6-acetyllysineBy similarity1
Modified residuei111N6-acetyllysine; alternateBy similarity1
Modified residuei111N6-malonyllysine; alternateBy similarity1
Modified residuei132PhosphoserineCombined sources1
Modified residuei272PhosphoserineBy similarity1
Modified residuei312N6-malonyllysineBy similarity1
Modified residuei330N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP05065
PRIDEiP05065

2D gel databases

World-2DPAGEi0004:P05065

PTM databases

iPTMnetiP05065
PhosphoSitePlusiP05065

Expressioni

Tissue specificityi

Expressed in muscle, brain and hepatoma cells.1 Publication

Gene expression databases

BgeeiENSRNOG00000052802 Expressed in 10 organ(s), highest expression level in skeletal muscle tissue
GenevisibleiP05065 RN

Interactioni

Subunit structurei

Homotetramer. Interacts with SNX9 and WAS. Interacts with FBP2; the interaction blocks FBP2 inhibition by physiological concentrations of AMP and reduces inhibition by Ca2+ (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Ppm1eQ80Z302EBI-522118,EBI-7473061

Protein-protein interaction databases

BioGridi246379, 3 interactors
IntActiP05065, 6 interactors
MINTiP05065
STRINGi10116.ENSRNOP00000032320

Structurei

3D structure databases

ProteinModelPortaliP05065
SMRiP05065
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1557 Eukaryota
COG3588 LUCA
GeneTreeiENSGT00390000010235
HOGENOMiHOG000220876
HOVERGENiHBG002386
InParanoidiP05065
KOiK01623
OMAiMILKPNM
OrthoDBiEOG091G0A9T
PhylomeDBiP05065

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR029768 Aldolase_I_AS
IPR013785 Aldolase_TIM
IPR000741 FBA_I
PfamiView protein in Pfam
PF00274 Glycolytic, 1 hit
PROSITEiView protein in PROSITE
PS00158 ALDOLASE_CLASS_I, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P05065-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPHPYPALTP EQKKELADIA HRIVAPGKGI LAADESTGSI AKRLQSIGTE
60 70 80 90 100
NTEENRRFYR QLLLTADDRV NPCIGGVILF HETLYQKADD GRPFPQVIKS
110 120 130 140 150
KGGVVGIKVD KGVVPLAGTN GETTTQGLDG LSERCAQYKK DGADFAKWRC
160 170 180 190 200
VLKIGEHTPS SLAIMENANV LARYASICQQ NGIVPIVEPE ILPDGDHDLK
210 220 230 240 250
RCQYVTEKVL AAVYKALSDH HVYLEGTLLK PNMVTPGHAC TQKFSNEEIA
260 270 280 290 300
MATVTALRRT VPPAVPGVTF LSGGQSEEEA SINLNAINKC PLLKPWALTF
310 320 330 340 350
SYGRALQASA LKAWGGKKEN LKAAQEEYIK RALANSLACQ GKYTPSGQSG
360
AAASESLFIS NHAY
Length:364
Mass (Da):39,352
Last modified:January 23, 2007 - v2
Checksum:i2774497C20DCC0DA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti145F → S in AAA40715 (PubMed:2416636).Curated1
Sequence conflicti165M → V in AAA40715 (PubMed:2416636).Curated1
Sequence conflicti330K → Q in AAA40720 (PubMed:6086339).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12919 mRNA Translation: AAA40714.1
M14420 mRNA Translation: AAA40715.1
X04261
, X04262, X04263, X04264 Genomic DNA Translation: CAA27815.1
BC064440 mRNA Translation: AAH64440.1
M28282 mRNA Translation: AAA40720.1
PIRiA24532 ADRTA
RefSeqiNP_001170776.1, NM_001177305.1
NP_001258465.1, NM_001271536.1
NP_036627.1, NM_012495.2
XP_006230273.1, XM_006230211.2
UniGeneiRn.1774
Rn.178420

Genome annotation databases

EnsembliENSRNOT00000080988; ENSRNOP00000068764; ENSRNOG00000052802
ENSRNOT00000087928; ENSRNOP00000069507; ENSRNOG00000052802
ENSRNOT00000088473; ENSRNOP00000069540; ENSRNOG00000052802
GeneIDi24189
KEGGirno:24189
UCSCiRGD:2089 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12919 mRNA Translation: AAA40714.1
M14420 mRNA Translation: AAA40715.1
X04261
, X04262, X04263, X04264 Genomic DNA Translation: CAA27815.1
BC064440 mRNA Translation: AAH64440.1
M28282 mRNA Translation: AAA40720.1
PIRiA24532 ADRTA
RefSeqiNP_001170776.1, NM_001177305.1
NP_001258465.1, NM_001271536.1
NP_036627.1, NM_012495.2
XP_006230273.1, XM_006230211.2
UniGeneiRn.1774
Rn.178420

3D structure databases

ProteinModelPortaliP05065
SMRiP05065
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi246379, 3 interactors
IntActiP05065, 6 interactors
MINTiP05065
STRINGi10116.ENSRNOP00000032320

PTM databases

iPTMnetiP05065
PhosphoSitePlusiP05065

2D gel databases

World-2DPAGEi0004:P05065

Proteomic databases

PaxDbiP05065
PRIDEiP05065

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000080988; ENSRNOP00000068764; ENSRNOG00000052802
ENSRNOT00000087928; ENSRNOP00000069507; ENSRNOG00000052802
ENSRNOT00000088473; ENSRNOP00000069540; ENSRNOG00000052802
GeneIDi24189
KEGGirno:24189
UCSCiRGD:2089 rat

Organism-specific databases

CTDi226
RGDi2089 Aldoa

Phylogenomic databases

eggNOGiKOG1557 Eukaryota
COG3588 LUCA
GeneTreeiENSGT00390000010235
HOGENOMiHOG000220876
HOVERGENiHBG002386
InParanoidiP05065
KOiK01623
OMAiMILKPNM
OrthoDBiEOG091G0A9T
PhylomeDBiP05065

Enzyme and pathway databases

UniPathwayi
UPA00109;UER00183

ReactomeiR-RNO-114608 Platelet degranulation
R-RNO-6798695 Neutrophil degranulation
R-RNO-70171 Glycolysis
R-RNO-70263 Gluconeogenesis
SABIO-RKiP05065

Miscellaneous databases

PROiPR:P05065

Gene expression databases

BgeeiENSRNOG00000052802 Expressed in 10 organ(s), highest expression level in skeletal muscle tissue
GenevisibleiP05065 RN

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR029768 Aldolase_I_AS
IPR013785 Aldolase_TIM
IPR000741 FBA_I
PfamiView protein in Pfam
PF00274 Glycolytic, 1 hit
PROSITEiView protein in PROSITE
PS00158 ALDOLASE_CLASS_I, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiALDOA_RAT
AccessioniPrimary (citable) accession number: P05065
Secondary accession number(s): Q63038
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: January 23, 2007
Last modified: October 10, 2018
This is version 163 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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