Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Alpha-ketoglutarate-dependent dioxygenase AlkB

Gene

alkB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Dioxygenase that repairs alkylated DNA and RNA containing 3-methylcytosine or 1-methyladenine by oxidative demethylation. Has highest activity towards 3-methylcytosine. Has lower activity towards alkylated DNA containing ethenoadenine, and no detectable activity towards 1-methylguanine or 3-methylthymine. Accepts double-stranded and single-stranded substrates. Requires molecular oxygen, alpha-ketoglutarate and iron. Provides extensive resistance to alkylating agents such as MMS and DMS (SN2 agents), but not to MMNG and MNU (SN1 agents).6 Publications

Catalytic activityi

DNA-base-CH3 + 2-oxoglutarate + O2 = DNA-base + formaldehyde + succinate + CO2.3 Publications

Cofactori

Fe2+PROSITE-ProRule annotation4 PublicationsNote: Binds 1 Fe2+ ion per subunit.PROSITE-ProRule annotation4 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei69Substrate2 Publications1
Metal bindingi131Iron; catalyticPROSITE-ProRule annotation1 Publication1
Metal bindingi133Iron; catalyticPROSITE-ProRule annotation1 Publication1
Binding sitei135Substrate2 Publications1
Binding sitei161Substrate2 Publications1
Metal bindingi187Iron; catalyticPROSITE-ProRule annotation1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDioxygenase, Oxidoreductase
Biological processDNA damage, DNA repair
LigandIron, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10037-MONOMER
MetaCyc:EG10037-MONOMER
BRENDAi1.14.11.33 2026

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-ketoglutarate-dependent dioxygenase AlkB (EC:1.14.11.33)
Alternative name(s):
Alkylated DNA repair protein AlkB
DNA oxidative demethylase AlkB
Gene namesi
Name:alkB
Synonyms:aidD
Ordered Locus Names:b2212, JW2200
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10037 alkB

Subcellular locationi

GO - Cellular componenti

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi51T → A: Slighly reduced activity towards single-stranded DNA containing 1-methyladenine. Reduces affinity for undamaged DNA. 1 Publication1
Mutagenesisi69W → A: Abolishes activity towards single-stranded DNA containing 1-methyladenine. 1 Publication1
Mutagenesisi76Y → A: Reduces affinity for damaged DNA and activity towards single-stranded DNA containing 1-methyladenine. 1 Publication1
Mutagenesisi135D → A: Abolishes activity towards single-stranded DNA containing 1-methyladenine. Alters substrate specificity, so that the enzyme gains activity towards single-stranded DNA containing 1-methylguanine. 1 Publication1
Mutagenesisi161R → A: No effect on enzyme activity. Decreases affinity for damaged DNA. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000666651 – 216Alpha-ketoglutarate-dependent dioxygenase AlkBAdd BLAST216

Proteomic databases

PaxDbiP05050
PRIDEiP05050

Interactioni

Protein-protein interaction databases

BioGridi4261918, 29 interactors
DIPiDIP-9085N
IntActiP05050, 12 interactors
STRINGi316385.ECDH10B_2369

Structurei

Secondary structure

1216
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP05050
SMRiP05050
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP05050

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini113 – 213Fe2OG dioxygenasePROSITE-ProRule annotationAdd BLAST101

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni76 – 78Substrate binding3
Regioni120 – 122Alpha-ketoglutarate binding3
Regioni204 – 210Alpha-ketoglutarate binding7

Sequence similaritiesi

Belongs to the alkB family.Curated

Phylogenomic databases

eggNOGiENOG4105CF1 Bacteria
COG3145 LUCA
HOGENOMiHOG000122276
InParanoidiP05050
KOiK03919
OMAiNCGPLGW
PhylomeDBiP05050

Family and domain databases

Gene3Di2.60.120.590, 1 hit
InterProiView protein in InterPro
IPR004574 Alkb
IPR027450 AlkB-like
IPR037151 AlkB-like_sf
IPR005123 Oxoglu/Fe-dep_dioxygenase
PANTHERiPTHR16557 PTHR16557, 1 hit
PfamiView protein in Pfam
PF13532 2OG-FeII_Oxy_2, 1 hit
TIGRFAMsiTIGR00568 alkb, 1 hit
PROSITEiView protein in PROSITE
PS51471 FE2OG_OXY, 1 hit

Sequencei

Sequence statusi: Complete.

P05050-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLDLFADAEP WQEPLAAGAV ILRRFAFNAA EQLIRDINDV ASQSPFRQMV
60 70 80 90 100
TPGGYTMSVA MTNCGHLGWT THRQGYLYSP IDPQTNKPWP AMPQSFHNLC
110 120 130 140 150
QRAATAAGYP DFQPDACLIN RYAPGAKLSL HQDKDEPDLR APIVSVSLGL
160 170 180 190 200
PAIFQFGGLK RNDPLKRLLL EHGDVVVWGG ESRLFYHGIQ PLKAGFHPLT
210
IDCRYNLTFR QAGKKE
Length:216
Mass (Da):24,076
Last modified:August 13, 1987 - v1
Checksum:i494FE1E70EABD278
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02607 Genomic DNA Translation: AAA23416.1
U00008 Genomic DNA Translation: AAA16409.1
U00096 Genomic DNA Translation: AAC75272.1
AP009048 Genomic DNA Translation: BAA15995.1
M10315 Genomic DNA Translation: AAA23414.1
PIRiA24605 BVECKB
RefSeqiNP_416716.1, NC_000913.3
WP_000884971.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75272; AAC75272; b2212
BAA15995; BAA15995; BAA15995
GeneIDi946708
KEGGiecj:JW2200
eco:b2212
PATRICifig|1411691.4.peg.23

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02607 Genomic DNA Translation: AAA23416.1
U00008 Genomic DNA Translation: AAA16409.1
U00096 Genomic DNA Translation: AAC75272.1
AP009048 Genomic DNA Translation: BAA15995.1
M10315 Genomic DNA Translation: AAA23414.1
PIRiA24605 BVECKB
RefSeqiNP_416716.1, NC_000913.3
WP_000884971.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FD8X-ray2.30A12-216[»]
2FDFX-ray2.10A12-216[»]
2FDGX-ray2.20A12-216[»]
2FDHX-ray2.10A12-216[»]
2FDIX-ray1.80A12-216[»]
2FDJX-ray2.10A12-216[»]
2FDKX-ray2.30A12-216[»]
3BI3X-ray1.90A13-213[»]
3BIEX-ray1.68A13-214[»]
3BKZX-ray1.65A14-214[»]
3I2OX-ray1.70A12-216[»]
3I3MX-ray1.50A12-216[»]
3I3QX-ray1.40A/B12-216[»]
3I49X-ray1.60A12-216[»]
3KHBX-ray2.90A/B1-216[»]
3KHCX-ray2.20A/B1-216[»]
3O1MX-ray1.75A12-216[»]
3O1OX-ray1.92A12-216[»]
3O1PX-ray1.51A12-216[»]
3O1RX-ray1.77A12-216[»]
3O1SX-ray1.58A12-216[»]
3O1TX-ray1.48A12-216[»]
3O1UX-ray1.54A12-216[»]
3O1VX-ray1.90A12-216[»]
3T3YX-ray2.00A12-216[»]
3T4HX-ray1.65B12-216[»]
3T4VX-ray1.73A12-216[»]
4JHTX-ray1.18A12-216[»]
4NIDX-ray1.58A12-216[»]
4NIGX-ray1.52A12-216[»]
4NIHX-ray1.37A12-216[»]
4NIIX-ray1.62A12-216[»]
4RFRX-ray1.50B14-216[»]
4ZHNX-ray1.33A12-216[»]
ProteinModelPortaliP05050
SMRiP05050
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261918, 29 interactors
DIPiDIP-9085N
IntActiP05050, 12 interactors
STRINGi316385.ECDH10B_2369

Proteomic databases

PaxDbiP05050
PRIDEiP05050

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75272; AAC75272; b2212
BAA15995; BAA15995; BAA15995
GeneIDi946708
KEGGiecj:JW2200
eco:b2212
PATRICifig|1411691.4.peg.23

Organism-specific databases

EchoBASEiEB0036
EcoGeneiEG10037 alkB

Phylogenomic databases

eggNOGiENOG4105CF1 Bacteria
COG3145 LUCA
HOGENOMiHOG000122276
InParanoidiP05050
KOiK03919
OMAiNCGPLGW
PhylomeDBiP05050

Enzyme and pathway databases

BioCyciEcoCyc:EG10037-MONOMER
MetaCyc:EG10037-MONOMER
BRENDAi1.14.11.33 2026

Miscellaneous databases

EvolutionaryTraceiP05050
PROiPR:P05050

Family and domain databases

Gene3Di2.60.120.590, 1 hit
InterProiView protein in InterPro
IPR004574 Alkb
IPR027450 AlkB-like
IPR037151 AlkB-like_sf
IPR005123 Oxoglu/Fe-dep_dioxygenase
PANTHERiPTHR16557 PTHR16557, 1 hit
PfamiView protein in Pfam
PF13532 2OG-FeII_Oxy_2, 1 hit
TIGRFAMsiTIGR00568 alkb, 1 hit
PROSITEiView protein in PROSITE
PS51471 FE2OG_OXY, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiALKB_ECOLI
AccessioniPrimary (citable) accession number: P05050
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: March 28, 2018
This is version 147 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again