UniProtKB - P05030 (PMA1_YEAST)
Protein
Plasma membrane ATPase 1
Gene
PMA1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
The plasma membrane ATPase of plants and fungi is a hydrogen ion pump. The proton gradient it generates drives the active transport of nutrients by H+-symport. The resulting external acidification and/or internal alkinization may mediate growth responses.
Miscellaneous
The prion state [GAR+] is provoked by the interaction of the two proteins STD1 and PMA1. It involves a complex between a small fraction of the cellular complement of PMA1, and STD1, a much lower-abundance protein, and it is transmissible by non-Mendelian, cytoplasmic inheritance. [GAR+] makes cells resistant to the glucose-associated repression of alternative carbon sources. In contrast to other prion forms, [GAR+] cannot be cured by GdnHCl or by inactivation of the molecular chaperone HSP104.1 Publication
There are two plasma membrane ATPases in yeast. This is the major isoform.
Present with 1260000 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- EC:7.1.2.1
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 378 | 4-aspartylphosphate intermediateBy similarity | 1 | |
Metal bindingi | 634 | MagnesiumBy similarity | 1 | |
Metal bindingi | 638 | MagnesiumBy similarity | 1 |
GO - Molecular functioni
- ATPase activity Source: GO_Central
- ATP binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- proton-exporting ATPase activity, phosphorylative mechanism Source: SGD
GO - Biological processi
- positive regulation of TORC1 signaling Source: SGD
- proteasome storage granule assembly Source: SGD
- proton export across plasma membrane Source: InterPro
- proton transmembrane transport Source: SGD
- regulation of intracellular pH Source: GO_Central
- transmembrane transport Source: SGD
Keywordsi
Molecular function | Prion, Translocase |
Biological process | Hydrogen ion transport, Ion transport, Transport |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | MetaCyc:G3O-30531-MONOMER |
BRENDAi | 3.6.3.6, 984 |
SABIO-RKi | P05030 |
Protein family/group databases
TCDBi | 3.A.3.3.6, the p-type atpase (p-atpase) superfamily |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:PMA1 Ordered Locus Names:YGL008C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000002976, PMA1 |
VEuPathDBi | FungiDB:YGL008C |
Subcellular locationi
Plasma membrane
Cytosol
- cytosol Source: SGD
Mitochondrion
- mitochondrion Source: SGD
Plasma Membrane
- plasma membrane Source: SGD
Other locations
- integral component of membrane Source: UniProtKB-KW
- membrane raft Source: SGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 115 | CytoplasmicSequence analysisAdd BLAST | 115 | |
Transmembranei | 116 – 136 | Helical; Name=1Sequence analysisAdd BLAST | 21 | |
Topological domaini | 137 – 140 | ExtracellularSequence analysis | 4 | |
Transmembranei | 141 – 160 | Helical; Name=2Sequence analysisAdd BLAST | 20 | |
Topological domaini | 161 – 291 | CytoplasmicSequence analysisAdd BLAST | 131 | |
Transmembranei | 292 – 313 | Helical; Name=3Sequence analysisAdd BLAST | 22 | |
Topological domaini | 314 – 325 | ExtracellularSequence analysisAdd BLAST | 12 | |
Transmembranei | 326 – 347 | Helical; Name=4Sequence analysisAdd BLAST | 22 | |
Topological domaini | 348 – 719 | CytoplasmicSequence analysisAdd BLAST | 372 | |
Transmembranei | 720 – 738 | Helical; Name=5Sequence analysisAdd BLAST | 19 | |
Topological domaini | 739 – 754 | ExtracellularSequence analysisAdd BLAST | 16 | |
Transmembranei | 755 – 774 | Helical; Name=6Sequence analysisAdd BLAST | 20 | |
Topological domaini | 775 – 824 | CytoplasmicSequence analysisAdd BLAST | 50 | |
Transmembranei | 825 – 845 | Helical; Name=7Sequence analysisAdd BLAST | 21 | |
Topological domaini | 846 – 857 | ExtracellularSequence analysisAdd BLAST | 12 | |
Transmembranei | 858 – 874 | Helical; Name=8Sequence analysisAdd BLAST | 17 | |
Topological domaini | 875 – 918 | CytoplasmicSequence analysisAdd BLAST | 44 |
Keywords - Cellular componenti
Amyloid, Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 129 | E → L or Q: Normal activity. 1 Publication | 1 | |
Mutagenesisi | 200 | D → N: Activity reduced to 23%. 1 Publication | 1 | |
Mutagenesisi | 233 | E → Q: Activity reduced to 33%. 1 Publication | 1 | |
Mutagenesisi | 271 | R → T: Normal activity. 1 Publication | 1 | |
Mutagenesisi | 335 | P → A: Activity reduced to 53%. 1 Publication | 1 | |
Mutagenesisi | 378 | D → E: Activity reduced to 67%. 1 Publication | 1 | |
Mutagenesisi | 378 | D → N: Activity reduced to 73%. 1 Publication | 1 | |
Mutagenesisi | 378 | D → T: Activity reduced to 49%. 1 Publication | 1 | |
Mutagenesisi | 474 | K → Q: Activity reduced to 19%. 1 Publication | 1 | |
Mutagenesisi | 534 | D → N: Activity reduced to 37%. 1 Publication | 1 | |
Mutagenesisi | 560 | D → N: Activity reduced to 24%. 1 Publication | 1 | |
Mutagenesisi | 638 | D → N: Activity reduced to 24%. 1 Publication | 1 | |
Mutagenesisi | 848 | N → D: Normal activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000046271 | 1 – 918 | Plasma membrane ATPase 1Add BLAST | 918 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 61 | PhosphoserineCombined sources | 1 | |
Modified residuei | 175 | PhosphothreonineCombined sources | 1 | |
Cross-linki | 252 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources | ||
Cross-linki | 555 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication | ||
Modified residuei | 911 | PhosphoserineCombined sources | 1 | |
Modified residuei | 912 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 918 | PhosphothreonineCombined sources | 1 |
Post-translational modificationi
Phosphorylated on multiple Ser and Thr residues.
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
MaxQBi | P05030 |
PaxDbi | P05030 |
PRIDEi | P05030 |
PTM databases
CarbonylDBi | P05030 |
iPTMneti | P05030 |
Interactioni
Subunit structurei
Interacts with its cargot receptor EXP1 for its transport within the cell and maturation.
1 PublicationProtein-protein interaction databases
BioGRIDi | 33238, 785 interactors |
DIPi | DIP-2537N |
IntActi | P05030, 23 interactors |
MINTi | P05030 |
STRINGi | 4932.YGL008C |
Miscellaneous databases
RNActi | P05030, protein |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 5 – 17 | Poly-SerAdd BLAST | 13 | |
Compositional biasi | 31 – 78 | Asp/Glu-rich (acidic)Add BLAST | 48 | |
Compositional biasi | 39 – 44 | Poly-Asp | 6 | |
Compositional biasi | 585 – 590 | Poly-Gly | 6 |
Sequence similaritiesi
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIIA subfamily. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0205, Eukaryota |
GeneTreei | ENSGT00940000176570 |
HOGENOMi | CLU_002360_6_0_1 |
InParanoidi | P05030 |
OMAi | FGVFCIM |
Family and domain databases
CDDi | cd02076, P-type_ATPase_H, 1 hit |
Gene3Di | 3.40.1110.10, 1 hit 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR004014, ATPase_P-typ_cation-transptr_N IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR023214, HAD_sf IPR006534, P-type_ATPase_IIIA IPR001757, P_typ_ATPase IPR044492, P_typ_ATPase_HD_dom |
Pfami | View protein in Pfam PF00690, Cation_ATPase_N, 1 hit |
PRINTSi | PR00120, HATPASE |
SFLDi | SFLDF00027, p-type_atpase, 1 hit |
SMARTi | View protein in SMART SM00831, Cation_ATPase_N, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01647, ATPase-IIIA_H, 1 hit TIGR01494, ATPase_P-type, 3 hits |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
i Sequence
Sequence statusi: Complete.
P05030-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTDTSSSSSS SSASSVSAHQ PTQEKPAKTY DDAASESSDD DDIDALIEEL
60 70 80 90 100
QSNHGVDDED SDNDGPVAAG EARPVPEEYL QTDPSYGLTS DEVLKRRKKY
110 120 130 140 150
GLNQMADEKE SLVVKFVMFF VGPIQFVMEA AAILAAGLSD WVDFGVICGL
160 170 180 190 200
LMLNAGVGFV QEFQAGSIVD ELKKTLANTA VVIRDGQLVE IPANEVVPGD
210 220 230 240 250
ILQLEDGTVI PTDGRIVTED CFLQIDQSAI TGESLAVDKH YGDQTFSSST
260 270 280 290 300
VKRGEGFMVV TATGDNTFVG RAAALVNKAA GGQGHFTEVL NGIGIILLVL
310 320 330 340 350
VIATLLLVWT ACFYRTNGIV RILRYTLGIT IIGVPVGLPA VVTTTMAVGA
360 370 380 390 400
AYLAKKQAIV QKLSAIESLA GVEILCSDKT GTLTKNKLSL HEPYTVEGVS
410 420 430 440 450
PDDLMLTACL AASRKKKGLD AIDKAFLKSL KQYPKAKDAL TKYKVLEFHP
460 470 480 490 500
FDPVSKKVTA VVESPEGERI VCVKGAPLFV LKTVEEDHPI PEDVHENYEN
510 520 530 540 550
KVAELASRGF RALGVARKRG EGHWEILGVM PCMDPPRDDT AQTVSEARHL
560 570 580 590 600
GLRVKMLTGD AVGIAKETCR QLGLGTNIYN AERLGLGGGG DMPGSELADF
610 620 630 640 650
VENADGFAEV FPQHKYRVVE ILQNRGYLVA MTGDGVNDAP SLKKADTGIA
660 670 680 690 700
VEGATDAARS AADIVFLAPG LSAIIDALKT SRQIFHRMYS YVVYRIALSL
710 720 730 740 750
HLEIFLGLWI AILDNSLDID LIVFIAIFAD VATLAIAYDN APYSPKPVKW
760 770 780 790 800
NLPRLWGMSI ILGIVLAIGS WITLTTMFLP KGGIIQNFGA MNGIMFLQIS
810 820 830 840 850
LTENWLIFIT RAAGPFWSSI PSWQLAGAVF AVDIIATMFT LFGWWSENWT
860 870 880 890 900
DIVTVVRVWI WSIGIFCVLG GFYYEMSTSE AFDRLMNGKP MKEKKSTRSV
910
EDFMAAMQRV STQHEKET
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X03534 Genomic DNA Translation: CAA27237.1 Z72530 Genomic DNA Translation: CAA96708.1 BK006941 Genomic DNA Translation: DAA08090.1 |
PIRi | S64010, PXBY1P |
RefSeqi | NP_011507.1, NM_001180873.1 |
Genome annotation databases
EnsemblFungii | YGL008C_mRNA; YGL008C; YGL008C |
GeneIDi | 852876 |
KEGGi | sce:YGL008C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X03534 Genomic DNA Translation: CAA27237.1 Z72530 Genomic DNA Translation: CAA96708.1 BK006941 Genomic DNA Translation: DAA08090.1 |
PIRi | S64010, PXBY1P |
RefSeqi | NP_011507.1, NM_001180873.1 |
3D structure databases
SMRi | P05030 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 33238, 785 interactors |
DIPi | DIP-2537N |
IntActi | P05030, 23 interactors |
MINTi | P05030 |
STRINGi | 4932.YGL008C |
Protein family/group databases
TCDBi | 3.A.3.3.6, the p-type atpase (p-atpase) superfamily |
PTM databases
CarbonylDBi | P05030 |
iPTMneti | P05030 |
Proteomic databases
MaxQBi | P05030 |
PaxDbi | P05030 |
PRIDEi | P05030 |
Genome annotation databases
EnsemblFungii | YGL008C_mRNA; YGL008C; YGL008C |
GeneIDi | 852876 |
KEGGi | sce:YGL008C |
Organism-specific databases
SGDi | S000002976, PMA1 |
VEuPathDBi | FungiDB:YGL008C |
Phylogenomic databases
eggNOGi | KOG0205, Eukaryota |
GeneTreei | ENSGT00940000176570 |
HOGENOMi | CLU_002360_6_0_1 |
InParanoidi | P05030 |
OMAi | FGVFCIM |
Enzyme and pathway databases
BioCyci | MetaCyc:G3O-30531-MONOMER |
BRENDAi | 3.6.3.6, 984 |
SABIO-RKi | P05030 |
Miscellaneous databases
PROi | PR:P05030 |
RNActi | P05030, protein |
Family and domain databases
CDDi | cd02076, P-type_ATPase_H, 1 hit |
Gene3Di | 3.40.1110.10, 1 hit 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR004014, ATPase_P-typ_cation-transptr_N IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR023214, HAD_sf IPR006534, P-type_ATPase_IIIA IPR001757, P_typ_ATPase IPR044492, P_typ_ATPase_HD_dom |
Pfami | View protein in Pfam PF00690, Cation_ATPase_N, 1 hit |
PRINTSi | PR00120, HATPASE |
SFLDi | SFLDF00027, p-type_atpase, 1 hit |
SMARTi | View protein in SMART SM00831, Cation_ATPase_N, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01647, ATPase-IIIA_H, 1 hit TIGR01494, ATPase_P-type, 3 hits |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PMA1_YEAST | |
Accessioni | P05030Primary (citable) accession number: P05030 Secondary accession number(s): D6VUC9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 13, 1987 |
Last sequence update: | November 1, 1995 | |
Last modified: | April 7, 2021 | |
This is version 219 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome VII
Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names - SIMILARITY comments
Index of protein domains and families