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Protein

D-ribose pyranase

Gene

rbsD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the interconversion of beta-pyran and beta-furan forms of D-ribose. It also catalyzes the conversion between beta-allofuranose and beta-allopyranose.1 Publication

Caution

Was originally thought (PubMed:3011793) to be a high affinity ribose transport protein, but further analysis (PubMed:15060078) shows that it is a D-ribose pyranase.2 Publications

Catalytic activityi

Beta-D-ribopyranose = beta-D-ribofuranose.1 Publication
Beta-D-allopyranose = beta-D-allofuranose.1 Publication

Pathwayi: D-ribose degradation

This protein is involved in step 1 of the subpathway that synthesizes D-ribose 5-phosphate from beta-D-ribopyranose.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. D-ribose pyranase (rbsD)
  2. Ribokinase (rbsK)
This subpathway is part of the pathway D-ribose degradation, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-ribose 5-phosphate from beta-D-ribopyranose, the pathway D-ribose degradation and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei20Proton donorCurated1
Binding sitei28SubstrateBy similarity1
Binding sitei106SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • D-ribose catabolic process Source: EcoCyc

Keywordsi

Molecular functionIsomerase
Biological processCarbohydrate metabolism

Enzyme and pathway databases

BioCyciEcoCyc:EG10817-MONOMER
MetaCyc:EG10817-MONOMER
BRENDAi5.4.99.62 2026
UniPathwayi
UPA00916;UER00888

Names & Taxonomyi

Protein namesi
Recommended name:
D-ribose pyranase (EC:5.4.99.621 Publication)
Gene namesi
Name:rbsD
Ordered Locus Names:b3748, JW5857
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10817 rbsD

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi20H → A: Loss of pyranase activity. 1 Publication1
Mutagenesisi106H → A: Retains one-third of the original activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000971921 – 139D-ribose pyranaseAdd BLAST139

Proteomic databases

EPDiP04982
PaxDbiP04982
PRIDEiP04982

Interactioni

Subunit structurei

Homodecamer.2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi4261236, 14 interactors
STRINGi316385.ECDH10B_3936

Structurei

3D structure databases

ProteinModelPortaliP04982
SMRiP04982
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni128 – 130Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the RbsD / FucU family. RbsD subfamily.Curated

Phylogenomic databases

eggNOGiENOG4105MRB Bacteria
COG1869 LUCA
HOGENOMiHOG000219040
InParanoidiP04982
KOiK06726
PhylomeDBiP04982

Family and domain databases

Gene3Di3.40.1650.10, 1 hit
HAMAPiMF_01661 D_rib_pyranase, 1 hit
InterProiView protein in InterPro
IPR023064 D-ribose_pyranase
IPR023750 RbsD-like_sf
IPR007721 RbsD_FucU
PANTHERiPTHR37831 PTHR37831, 1 hit
PfamiView protein in Pfam
PF05025 RbsD_FucU, 1 hit
SUPFAMiSSF102546 SSF102546, 1 hit

Sequencei

Sequence statusi: Complete.

P04982-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKKGTVLNSD ISSVISRLGH TDTLVVCDAG LPIPKSTTRI DMALTQGVPS
60 70 80 90 100
FMQVLGVVTN EMQVEAAIIA EEIKHHNPQL HETLLTHLEQ LQKHQGNTIE
110 120 130
IRYTTHEQFK QQTAESQAVI RSGECSPYAN IILCAGVTF
Length:139
Mass (Da):15,292
Last modified:July 19, 2004 - v3
Checksum:iD19A9D98CB79F611
GO

Sequence cautioni

The sequence AAA62101 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13169 Genomic DNA Translation: AAA51472.1
L10328 Genomic DNA Translation: AAA62101.1 Different initiation.
U00096 Genomic DNA Translation: AAC76771.2
AP009048 Genomic DNA Translation: BAE77540.1
X68551 Genomic DNA Translation: CAA48556.1
PIRiE65178
RefSeqiNP_418204.2, NC_000913.3
WP_001301979.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76771; AAC76771; b3748
BAE77540; BAE77540; BAE77540
GeneIDi948267
KEGGiecj:JW5857
eco:b3748
PATRICifig|1411691.4.peg.2952

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13169 Genomic DNA Translation: AAA51472.1
L10328 Genomic DNA Translation: AAA62101.1 Different initiation.
U00096 Genomic DNA Translation: AAC76771.2
AP009048 Genomic DNA Translation: BAE77540.1
X68551 Genomic DNA Translation: CAA48556.1
PIRiE65178
RefSeqiNP_418204.2, NC_000913.3
WP_001301979.1, NZ_LN832404.1

3D structure databases

ProteinModelPortaliP04982
SMRiP04982
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261236, 14 interactors
STRINGi316385.ECDH10B_3936

Proteomic databases

EPDiP04982
PaxDbiP04982
PRIDEiP04982

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76771; AAC76771; b3748
BAE77540; BAE77540; BAE77540
GeneIDi948267
KEGGiecj:JW5857
eco:b3748
PATRICifig|1411691.4.peg.2952

Organism-specific databases

EchoBASEiEB0810
EcoGeneiEG10817 rbsD

Phylogenomic databases

eggNOGiENOG4105MRB Bacteria
COG1869 LUCA
HOGENOMiHOG000219040
InParanoidiP04982
KOiK06726
PhylomeDBiP04982

Enzyme and pathway databases

UniPathwayi
UPA00916;UER00888

BioCyciEcoCyc:EG10817-MONOMER
MetaCyc:EG10817-MONOMER
BRENDAi5.4.99.62 2026

Miscellaneous databases

PROiPR:P04982

Family and domain databases

Gene3Di3.40.1650.10, 1 hit
HAMAPiMF_01661 D_rib_pyranase, 1 hit
InterProiView protein in InterPro
IPR023064 D-ribose_pyranase
IPR023750 RbsD-like_sf
IPR007721 RbsD_FucU
PANTHERiPTHR37831 PTHR37831, 1 hit
PfamiView protein in Pfam
PF05025 RbsD_FucU, 1 hit
SUPFAMiSSF102546 SSF102546, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRBSD_ECOLI
AccessioniPrimary (citable) accession number: P04982
Secondary accession number(s): Q2M866
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: July 19, 2004
Last modified: November 7, 2018
This is version 138 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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