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Protein

Thrombin-like enzyme batroxobin

Gene
N/A
Organism
Bothrops atrox (Barba amarilla) (Fer-de-lance)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Thrombin-like snake venom serine protease. Cleaves Arg-Gly bonds in fibrinogen alpha chains (FGA).1 Publication

Miscellaneous

Does not cleave beta-chains of fibrinogen (FGB).

Catalytic activityi

Selective cleavage of Arg-|-Xaa bond in fibrinogen, to form fibrin, and release fibrinopeptide A. The specificity of further degradation of fibrinogen varies with species origin of the enzyme.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei65Charge relay systemBy similarity1
Active sitei110Charge relay systemBy similarity1
Sitei196Required for specificityBy similarity1
Active sitei202Charge relay systemBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionBlood coagulation cascade activating toxin, Hemostasis impairing toxin, Hydrolase, Protease, Serine protease, Toxin

Protein family/group databases

MEROPSiS01.176

Names & Taxonomyi

Protein namesi
Recommended name:
Thrombin-like enzyme batroxobin (EC:3.4.21.74)
Short name:
BX
Short name:
SVTLE
Alternative name(s):
Bothrops atrox serine proteinase
Defibrase
Fibrinogen-clotting enzyme
Reptilase
Snake venom serine protease
Short name:
SVSP
Venombin A
OrganismiBothrops atrox (Barba amarilla) (Fer-de-lance)
Taxonomic identifieri8725 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaViperidaeCrotalinaeBothrops

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Biotechnological usei

Is used for the investigation of the last phase of blood coagulation. Due to its heparin insensitivity it can detect fibrinogen polymerization disorders even in the presence of heparin.

Pharmaceutical usei

Available under the name Defibrase (Pentapharm) for the treatment of thrombotic diseases.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18By similarityAdd BLAST18
PropeptideiPRO_000002837919 – 246
ChainiPRO_000002838025 – 255Thrombin-like enzyme batroxobinAdd BLAST231

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi31 ↔ 163PROSITE-ProRule annotation
Disulfide bondi50 ↔ 66PROSITE-ProRule annotation
Disulfide bondi98 ↔ 254PROSITE-ProRule annotation
Disulfide bondi142 ↔ 208PROSITE-ProRule annotation
Glycosylationi170N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi174 ↔ 187PROSITE-ProRule annotation
Disulfide bondi198 ↔ 223PROSITE-ProRule annotation
Glycosylationi249N-linked (GlcNAc...) asparagine1 Publication1

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

PTM databases

GlyConnecti68

Expressioni

Tissue specificityi

Expressed by the venom gland.

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP04971
SMRiP04971
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 247Peptidase S1PROSITE-ProRule annotationAdd BLAST223

Sequence similaritiesi

Belongs to the peptidase S1 family. Snake venom subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG013304
KOiK20137

Family and domain databases

CDDicd00190 Tryp_SPc, 1 hit
InterProiView protein in InterPro
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
PfamiView protein in Pfam
PF00089 Trypsin, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
PROSITEiView protein in PROSITE
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04971-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVLIRVIANL LILQVSYAQK SSELVIGGDE CDINEHPFLA FMYYSPRYFC
60 70 80 90 100
GMTLINQEWV LTAAHCNRRF MRIHLGKHAG SVANYDEVVR YPKEKFICPN
110 120 130 140 150
KKKNVITDKD IMLIRLDRPV KNSEHIAPLS LPSNPPSVGS VCRIMGWGAI
160 170 180 190 200
TTSEDTYPDV PHCANINLFN NTVCREAYNG LPAKTLCAGV LQGGIDTCGG
210 220 230 240 250
DSGGPLICNG QFQGILSWGS DPCAEPRKPA FYTKVFDYLP WIQSIIAGNK

TATCP
Length:255
Mass (Da):28,189
Last modified:August 13, 1987 - v1
Checksum:iBE155BBC5DF8431B
GO

Sequence cautioni

The sequence AAA48553 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02684 mRNA Translation: AAA48552.1
X12747 Genomic DNA Translation: CAA31240.1
M20894
, M20890, M20891, M20892, M20893 Genomic DNA Translation: AAA48553.1 Sequence problems.
PIRiA28169

Genome annotation databases

KEGGiag:AAA48552

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02684 mRNA Translation: AAA48552.1
X12747 Genomic DNA Translation: CAA31240.1
M20894
, M20890, M20891, M20892, M20893 Genomic DNA Translation: AAA48553.1 Sequence problems.
PIRiA28169

3D structure databases

ProteinModelPortaliP04971
SMRiP04971
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiS01.176

PTM databases

GlyConnecti68

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:AAA48552

Phylogenomic databases

HOVERGENiHBG013304
KOiK20137

Family and domain databases

CDDicd00190 Tryp_SPc, 1 hit
InterProiView protein in InterPro
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
PfamiView protein in Pfam
PF00089 Trypsin, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
PROSITEiView protein in PROSITE
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiVSPF_BOTAT
AccessioniPrimary (citable) accession number: P04971
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: April 25, 2018
This is version 106 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

Pharmaceutical

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Peptidase families
    Classification of peptidase families and list of entries
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