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Protein

Tetanus toxin

Gene

tetX

Organism
Clostridium tetani (strain Massachusetts / E88)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Tetanus toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that catalyzes the hydrolysis of the '76-Gln-|-Phe-77' bond of synaptobrevin-2.

Miscellaneous

The C-terminus of the heavy chain binds to ganglioside receptors.

Catalytic activityi

Hydrolysis of 76-Gln-|-Phe-77 bond in synaptobrevin 2.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi233Zinc; catalyticPROSITE-ProRule annotation1
Active sitei234PROSITE-ProRule annotation1
Metal bindingi237Zinc; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

  • metalloendopeptidase activity Source: UniProtKB
  • protein transmembrane transporter activity Source: Reactome
  • toxin activity Source: UniProtKB-KW
  • zinc ion binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Metalloprotease, Neurotoxin, Protease, Toxin
Biological processVirulence
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciCTET212717:G1G04-2830-MONOMER
BRENDAi3.4.24.68 1526
ReactomeiR-HSA-5250982 Toxicity of tetanus toxin (TeNT)

Protein family/group databases

MEROPSiM27.001
TCDBi1.C.8.1.2 the botulinum and tetanus toxin (btt) family
UniLectiniP04958

Names & Taxonomyi

Protein namesi
Recommended name:
Tetanus toxin (EC:3.4.24.68)
Alternative name(s):
Tentoxylysin
Cleaved into the following 2 chains:
Tetanus toxin light chain
Short name:
Tetanus toxin chain L
Tetanus toxin heavy chain
Short name:
Tetanus toxin chain H
Gene namesi
Name:tetX
Ordered Locus Names:CTC_p60
Encoded oniPlasmid pE881 Publication
Plasmid 75 Kbp3 Publications
OrganismiClostridium tetani (strain Massachusetts / E88)
Taxonomic identifieri212717 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
Proteomesi
  • UP000001412 Componenti: Plasmid pE88

Subcellular locationi

GO - Cellular componenti

Pathology & Biotechi

Protein family/group databases

Allergomei2757 Clo t Toxoid

Chemistry databases

ChEMBLiCHEMBL2036
DrugBankiDB02379 Beta-D-Glucose
DB02265 Ethyl-Trimethyl-Silane
DB04465 Lactose
DB03721 N-acetyl-alpha-neuraminic acid
DB04265 N-acetyl-beta-neuraminic acid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00000292292 – 457Tetanus toxin light chainAdd BLAST456
ChainiPRO_0000029230458 – 1315Tetanus toxin heavy chainAdd BLAST858

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi439 ↔ 467Interchain (between light and heavy chains)1 Publication
Disulfide bondi1077 ↔ 10931 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP04958

Miscellaneous databases

PMAP-CutDBiP04958

Interactioni

Subunit structurei

The precursor polypeptide is subsequently cleaved to yield subchains L and H. These remain linked by a disulfide bridge and are non-toxic after separation.

Binary interactionsi

WithEntry#Exp.IntActNotes
HSPA1AP0DMV84EBI-13951617,EBI-11052499From Homo sapiens.

Protein-protein interaction databases

IntActiP04958, 2 interactors

Chemistry databases

BindingDBiP04958

Structurei

Secondary structure

11315
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP04958
SMRiP04958
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04958

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M27 family.Curated

Phylogenomic databases

KOiK08644
OMAiIHNEYTI
OrthoDBiPOG091H19UB

Family and domain databases

Gene3Di1.20.1120.10, 1 hit
InterProiView protein in InterPro
IPR000395 Bot/tetX_LC
IPR036248 Clostridium_toxin_transloc
IPR013320 ConA-like_dom_sf
IPR011065 Kunitz_inhibitor_STI-like_sf
IPR013104 Toxin_rcpt-bd_C
IPR012928 Toxin_rcpt-bd_N
IPR012500 Toxin_trans
PfamiView protein in Pfam
PF01742 Peptidase_M27, 1 hit
PF07951 Toxin_R_bind_C, 1 hit
PF07953 Toxin_R_bind_N, 1 hit
PF07952 Toxin_trans, 1 hit
PRINTSiPR00760 BONTOXILYSIN
SUPFAMiSSF49899 SSF49899, 1 hit
SSF50386 SSF50386, 1 hit
SSF58091 SSF58091, 1 hit
PROSITEiView protein in PROSITE
PS00142 ZINC_PROTEASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04958-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPITINNFRY SDPVNNDTII MMEPPYCKGL DIYYKAFKIT DRIWIVPERY
60 70 80 90 100
EFGTKPEDFN PPSSLIEGAS EYYDPNYLRT DSDKDRFLQT MVKLFNRIKN
110 120 130 140 150
NVAGEALLDK IINAIPYLGN SYSLLDKFDT NSNSVSFNLL EQDPSGATTK
160 170 180 190 200
SAMLTNLIIF GPGPVLNKNE VRGIVLRVDN KNYFPCRDGF GSIMQMAFCP
210 220 230 240 250
EYVPTFDNVI ENITSLTIGK SKYFQDPALL LMHELIHVLH GLYGMQVSSH
260 270 280 290 300
EIIPSKQEIY MQHTYPISAE ELFTFGGQDA NLISIDIKND LYEKTLNDYK
310 320 330 340 350
AIANKLSQVT SCNDPNIDID SYKQIYQQKY QFDKDSNGQY IVNEDKFQIL
360 370 380 390 400
YNSIMYGFTE IELGKKFNIK TRLSYFSMNH DPVKIPNLLD DTIYNDTEGF
410 420 430 440 450
NIESKDLKSE YKGQNMRVNT NAFRNVDGSG LVSKLIGLCK KIIPPTNIRE
460 470 480 490 500
NLYNRTASLT DLGGELCIKI KNEDLTFIAE KNSFSEEPFQ DEIVSYNTKN
510 520 530 540 550
KPLNFNYSLD KIIVDYNLQS KITLPNDRTT PVTKGIPYAP EYKSNAASTI
560 570 580 590 600
EIHNIDDNTI YQYLYAQKSP TTLQRITMTN SVDDALINST KIYSYFPSVI
610 620 630 640 650
SKVNQGAQGI LFLQWVRDII DDFTNESSQK TTIDKISDVS TIVPYIGPAL
660 670 680 690 700
NIVKQGYEGN FIGALETTGV VLLLEYIPEI TLPVIAALSI AESSTQKEKI
710 720 730 740 750
IKTIDNFLEK RYEKWIEVYK LVKAKWLGTV NTQFQKRSYQ MYRSLEYQVD
760 770 780 790 800
AIKKIIDYEY KIYSGPDKEQ IADEINNLKN KLEEKANKAM ININIFMRES
810 820 830 840 850
SRSFLVNQMI NEAKKQLLEF DTQSKNILMQ YIKANSKFIG ITELKKLESK
860 870 880 890 900
INKVFSTPIP FSYSKNLDCW VDNEEDIDVI LKKSTILNLD INNDIISDIS
910 920 930 940 950
GFNSSVITYP DAQLVPGING KAIHLVNNES SEVIVHKAMD IEYNDMFNNF
960 970 980 990 1000
TVSFWLRVPK VSASHLEQYG TNEYSIISSM KKHSLSIGSG WSVSLKGNNL
1010 1020 1030 1040 1050
IWTLKDSAGE VRQITFRDLP DKFNAYLANK WVFITITNDR LSSANLYING
1060 1070 1080 1090 1100
VLMGSAEITG LGAIREDNNI TLKLDRCNNN NQYVSIDKFR IFCKALNPKE
1110 1120 1130 1140 1150
IEKLYTSYLS ITFLRDFWGN PLRYDTEYYL IPVASSSKDV QLKNITDYMY
1160 1170 1180 1190 1200
LTNAPSYTNG KLNIYYRRLY NGLKFIIKRY TPNNEIDSFV KSGDFIKLYV
1210 1220 1230 1240 1250
SYNNNEHIVG YPKDGNAFNN LDRILRVGYN APGIPLYKKM EAVKLRDLKT
1260 1270 1280 1290 1300
YSVQLKLYDD KNASLGLVGT HNGQIGNDPN RDILIASNWY FNHLKDKILG
1310
CDWYFVPTDE GWTND
Length:1,315
Mass (Da):150,682
Last modified:January 23, 2007 - v2
Checksum:i18838FB2654024CF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04436 Genomic DNA Translation: CAA28033.1
X06214 Genomic DNA Translation: CAA29564.1
AF528097 Genomic DNA Translation: AAO37454.1
M12739 Genomic DNA Translation: AAA23282.1
PIRiA25689 BTCLTN
RefSeqiWP_011100836.1, NC_004565.1

Genome annotation databases

EnsemblBacteriaiAAO37454; AAO37454; CTC_p60
GeneIDi24255210
KEGGictc:CTC_p60

Similar proteinsi

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - Glycan Binding

Tetanus toxin Hc fragment

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04436 Genomic DNA Translation: CAA28033.1
X06214 Genomic DNA Translation: CAA29564.1
AF528097 Genomic DNA Translation: AAO37454.1
M12739 Genomic DNA Translation: AAA23282.1
PIRiA25689 BTCLTN
RefSeqiWP_011100836.1, NC_004565.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A8DX-ray1.57A865-1315[»]
1AF9X-ray2.70A865-1315[»]
1D0HX-ray2.10A872-1315[»]
1DFQX-ray2.60A872-1315[»]
1DIWX-ray2.00A875-1315[»]
1DLLX-ray1.80A875-1315[»]
1FV2X-ray2.50A862-1315[»]
1FV3X-ray2.30A/B862-1315[»]
1YVGX-ray2.60A2-458[»]
1YXWX-ray2.20A875-1315[»]
1YYNX-ray2.30A875-1315[»]
1Z7HX-ray2.30A1-443[»]
3HMYX-ray2.00A866-1315[»]
3HN1X-ray2.10A866-1315[»]
4J1LX-ray2.60A2-427[»]
5N0BX-ray2.60A1-1315[»]
5N0CX-ray2.60A/B1-1315[»]
ProteinModelPortaliP04958
SMRiP04958
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP04958, 2 interactors

Chemistry databases

BindingDBiP04958
ChEMBLiCHEMBL2036
DrugBankiDB02379 Beta-D-Glucose
DB02265 Ethyl-Trimethyl-Silane
DB04465 Lactose
DB03721 N-acetyl-alpha-neuraminic acid
DB04265 N-acetyl-beta-neuraminic acid

Protein family/group databases

Allergomei2757 Clo t Toxoid
MEROPSiM27.001
TCDBi1.C.8.1.2 the botulinum and tetanus toxin (btt) family
UniLectiniP04958

Proteomic databases

PRIDEiP04958

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO37454; AAO37454; CTC_p60
GeneIDi24255210
KEGGictc:CTC_p60

Phylogenomic databases

KOiK08644
OMAiIHNEYTI
OrthoDBiPOG091H19UB

Enzyme and pathway databases

BioCyciCTET212717:G1G04-2830-MONOMER
BRENDAi3.4.24.68 1526
ReactomeiR-HSA-5250982 Toxicity of tetanus toxin (TeNT)

Miscellaneous databases

EvolutionaryTraceiP04958
PMAP-CutDBiP04958
PROiPR:P04958

Family and domain databases

Gene3Di1.20.1120.10, 1 hit
InterProiView protein in InterPro
IPR000395 Bot/tetX_LC
IPR036248 Clostridium_toxin_transloc
IPR013320 ConA-like_dom_sf
IPR011065 Kunitz_inhibitor_STI-like_sf
IPR013104 Toxin_rcpt-bd_C
IPR012928 Toxin_rcpt-bd_N
IPR012500 Toxin_trans
PfamiView protein in Pfam
PF01742 Peptidase_M27, 1 hit
PF07951 Toxin_R_bind_C, 1 hit
PF07953 Toxin_R_bind_N, 1 hit
PF07952 Toxin_trans, 1 hit
PRINTSiPR00760 BONTOXILYSIN
SUPFAMiSSF49899 SSF49899, 1 hit
SSF50386 SSF50386, 1 hit
SSF58091 SSF58091, 1 hit
PROSITEiView protein in PROSITE
PS00142 ZINC_PROTEASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTETX_CLOTE
AccessioniPrimary (citable) accession number: P04958
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 166 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Plasmid, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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