UniProtKB - P04951 (KDSB_ECOLI)
Protein
3-deoxy-manno-octulosonate cytidylyltransferase
Gene
kdsB
Organism
Escherichia coli (strain K12)
Status
Functioni
Activates KDO (a required 8-carbon sugar) for incorporation into bacterial lipopolysaccharide in Gram-negative bacteria.UniRule annotation
Catalytic activityi
- 3-deoxy-α-D-manno-oct-2-ulosonate + CTP = CMP-3-deoxy-β-D-manno-octulosonate + diphosphateUniRule annotationEC:2.7.7.38UniRule annotation
Cofactori
: CMP-3-deoxy-D-manno-octulosonate biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes CMP-3-deoxy-D-manno-octulosonate from 3-deoxy-D-manno-octulosonate and CTP.UniRule annotationProteins known to be involved in this subpathway in this organism are:
- 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB), 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CMP-3-deoxy-D-manno-octulosonate from 3-deoxy-D-manno-octulosonate and CTP, the pathway CMP-3-deoxy-D-manno-octulosonate biosynthesis and in Nucleotide-sugar biosynthesis.
Pathwayi: lipopolysaccharide biosynthesis
This protein is involved in the pathway lipopolysaccharide biosynthesis, which is part of Bacterial outer membrane biogenesis.UniRule annotationView all proteins of this organism that are known to be involved in the pathway lipopolysaccharide biosynthesis and in Bacterial outer membrane biogenesis.
GO - Molecular functioni
- 3-deoxy-manno-octulosonate cytidylyltransferase activity Source: EcoCyc
- magnesium ion binding Source: EcoCyc
GO - Biological processi
- CMP-keto-3-deoxy-D-manno-octulosonic acid biosynthetic process Source: UniProtKB-UniRule
- keto-3-deoxy-D-manno-octulosonic acid biosynthetic process Source: EcoCyc
Keywordsi
Molecular function | Nucleotidyltransferase, Transferase |
Biological process | Lipopolysaccharide biosynthesis |
Ligand | Magnesium |
Enzyme and pathway databases
BioCyci | EcoCyc:CPM-KDOSYNTH-MONOMER MetaCyc:CPM-KDOSYNTH-MONOMER |
UniPathwayi | UPA00030 UPA00358;UER00476 |
Names & Taxonomyi
Protein namesi | Recommended name: 3-deoxy-manno-octulosonate cytidylyltransferaseUniRule annotation (EC:2.7.7.38UniRule annotation)Alternative name(s): CMP-2-keto-3-deoxyoctulosonic acid synthaseUniRule annotation Short name: CKSUniRule annotation Short name: CMP-KDO synthaseUniRule annotation |
Gene namesi | Name:kdsBUniRule annotation Ordered Locus Names:b0918, JW0901 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Cytosol
- cytosol Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed | |||
ChainiPRO_0000188501 | 2 – 248 | 3-deoxy-manno-octulosonate cytidylyltransferaseAdd BLAST | 247 |
Proteomic databases
jPOSTi | P04951 |
PaxDbi | P04951 |
PRIDEi | P04951 |
2D gel databases
SWISS-2DPAGEi | P04951 |
Interactioni
Binary interactionsi
Hide detailsP04951
With | #Exp. | IntAct |
---|---|---|
nemR [P67430] | 3 | EBI-544810,EBI-544803 |
rutR [P0ACU2] | 2 | EBI-544810,EBI-1121539 |
tufA [P0CE47] | 2 | EBI-544810,EBI-301077 |
Protein-protein interaction databases
BioGRIDi | 4260010, 226 interactors 849913, 2 interactors |
DIPi | DIP-10066N |
IntActi | P04951, 20 interactors |
STRINGi | 511145.b0918 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P04951 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P04951 |
Family & Domainsi
Sequence similaritiesi
Belongs to the KdsB family.UniRule annotation
Phylogenomic databases
eggNOGi | COG1212, Bacteria |
HOGENOMi | CLU_065038_1_0_6 |
InParanoidi | P04951 |
PhylomeDBi | P04951 |
Family and domain databases
CDDi | cd02517, CMP-KDO-Synthetase, 1 hit |
Gene3Di | 3.90.550.10, 1 hit |
HAMAPi | MF_00057, KdsB, 1 hit |
InterProi | View protein in InterPro IPR003329, Cytidylyl_trans IPR004528, KdsB IPR029044, Nucleotide-diphossugar_trans |
Pfami | View protein in Pfam PF02348, CTP_transf_3, 1 hit |
SUPFAMi | SSF53448, SSF53448, 1 hit |
TIGRFAMsi | TIGR00466, kdsB, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P04951-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSFVVIIPAR YASTRLPGKP LVDINGKPMI VHVLERARES GAERIIVATD
60 70 80 90 100
HEDVARAVEA AGGEVCMTRA DHQSGTERLA EVVEKCAFSD DTVIVNVQGD
110 120 130 140 150
EPMIPATIIR QVADNLAQRQ VGMATLAVPI HNAEEAFNPN AVKVVLDAEG
160 170 180 190 200
YALYFSRATI PWDRDRFAEG LETVGDNFLR HLGIYGYRAG FIRRYVNWQP
210 220 230 240
SPLEHIEMLE QLRVLWYGEK IHVAVAQEVP GTGVDTPEDL ERVRAEMR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J02614 Genomic DNA Translation: AAA83877.1 U00096 Genomic DNA Translation: AAC74004.1 AP009048 Genomic DNA Translation: BAA35664.1 |
PIRi | A26322 |
RefSeqi | NP_415438.1, NC_000913.3 WP_000011603.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC74004; AAC74004; b0918 BAA35664; BAA35664; BAA35664 |
GeneIDi | 945539 |
KEGGi | ecj:JW0901 eco:b0918 |
PATRICi | fig|1411691.4.peg.1358 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J02614 Genomic DNA Translation: AAA83877.1 U00096 Genomic DNA Translation: AAC74004.1 AP009048 Genomic DNA Translation: BAA35664.1 |
PIRi | A26322 |
RefSeqi | NP_415438.1, NC_000913.3 WP_000011603.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1VH1 | X-ray | 2.60 | A/B/C/D | 2-248 | [»] | |
3K8D | X-ray | 1.90 | A/B/C/D | 1-248 | [»] | |
3K8E | X-ray | 2.51 | A/B/C/D | 1-248 | [»] | |
SMRi | P04951 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260010, 226 interactors 849913, 2 interactors |
DIPi | DIP-10066N |
IntActi | P04951, 20 interactors |
STRINGi | 511145.b0918 |
2D gel databases
SWISS-2DPAGEi | P04951 |
Proteomic databases
jPOSTi | P04951 |
PaxDbi | P04951 |
PRIDEi | P04951 |
Genome annotation databases
EnsemblBacteriai | AAC74004; AAC74004; b0918 BAA35664; BAA35664; BAA35664 |
GeneIDi | 945539 |
KEGGi | ecj:JW0901 eco:b0918 |
PATRICi | fig|1411691.4.peg.1358 |
Organism-specific databases
EchoBASEi | EB0514 |
Phylogenomic databases
eggNOGi | COG1212, Bacteria |
HOGENOMi | CLU_065038_1_0_6 |
InParanoidi | P04951 |
PhylomeDBi | P04951 |
Enzyme and pathway databases
UniPathwayi | UPA00030 UPA00358;UER00476 |
BioCyci | EcoCyc:CPM-KDOSYNTH-MONOMER MetaCyc:CPM-KDOSYNTH-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P04951 |
PROi | PR:P04951 |
Family and domain databases
CDDi | cd02517, CMP-KDO-Synthetase, 1 hit |
Gene3Di | 3.90.550.10, 1 hit |
HAMAPi | MF_00057, KdsB, 1 hit |
InterProi | View protein in InterPro IPR003329, Cytidylyl_trans IPR004528, KdsB IPR029044, Nucleotide-diphossugar_trans |
Pfami | View protein in Pfam PF02348, CTP_transf_3, 1 hit |
SUPFAMi | SSF53448, SSF53448, 1 hit |
TIGRFAMsi | TIGR00466, kdsB, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KDSB_ECOLI | |
Accessioni | P04951Primary (citable) accession number: P04951 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 13, 1987 |
Last sequence update: | January 23, 2007 | |
Last modified: | April 7, 2021 | |
This is version 179 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families