UniProtKB - P04919 (B3AT_MOUSE)
Band 3 anion transport protein
Slc4a1
Functioni
Functions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein. Major integral membrane glycoprotein of the erythrocyte membrane; required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin. Functions as a transporter that mediates the 1:1 exchange of inorganic anions across the erythrocyte membrane. Mediates chloride-bicarbonate exchange in the kidney, and is required for normal acidification of the urine.
By similarityGO - Molecular functioni
- actin binding Source: MGI
- anion:anion antiporter activity Source: MGI
- ankyrin binding Source: MGI
- bicarbonate transmembrane transporter activity Source: MGI
- chloride transmembrane transporter activity Source: UniProtKB
- enzyme binding Source: MGI
- hemoglobin binding Source: MGI
- inorganic anion exchanger activity Source: UniProtKB
- protein-containing complex binding Source: MGI
- protein C-terminus binding Source: MGI
- protein homodimerization activity Source: MGI
- transmembrane transporter activity Source: GO_Central
GO - Biological processi
- anion transport Source: MGI
- bicarbonate transport Source: MGI
- blood coagulation Source: MGI
- chloride transport Source: UniProtKB
- erythrocyte development Source: MGI
- ion homeostasis Source: GO_Central
- negative regulation of glycolytic process through fructose-6-phosphate Source: MGI
- negative regulation of oxidoreductase activity Source: MGI
- negative regulation of urine volume Source: MGI
- pH elevation Source: MGI
- plasma membrane phospholipid scrambling Source: MGI
- positive regulation of T cell proliferation Source: MGI
- protein localization to plasma membrane Source: MGI
- regulation of intracellular pH Source: GO_Central
- response to activity Source: MGI
- response to arsenic-containing substance Source: MGI
- response to carbon dioxide Source: MGI
- response to hydrogen peroxide Source: MGI
- transmembrane transport Source: GO_Central
Keywordsi
Biological process | Anion exchange, Ion transport, Transport |
Enzyme and pathway databases
Reactomei | R-MMU-1237044, Erythrocytes take up carbon dioxide and release oxygen R-MMU-1247673, Erythrocytes take up oxygen and release carbon dioxide R-MMU-425381, Bicarbonate transporters |
Names & Taxonomyi
Protein namesi | Recommended name: Band 3 anion transport proteinAlternative name(s): Anion exchange protein 1 Short name: AE 1 Short name: Anion exchanger 1 MEB3 Solute carrier family 4 member 1 CD_antigen: CD233 |
Gene namesi | Name:Slc4a1 Synonyms:Ae1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:109393, Slc4a1 |
VEuPathDBi | HostDB:ENSMUSG00000006574 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Multi-pass membrane protein By similarity
- Basolateral cell membrane By similarity; Multi-pass membrane protein By similarity
Note: Detected in the erythrocyte cell membrane and on the basolateral membrane of alpha-intercalated cells in the collecting duct in the kidney.By similarity
Cytoskeleton
- cortical cytoskeleton Source: MGI
Plasma Membrane
- basolateral plasma membrane Source: UniProtKB
- cytoplasmic side of plasma membrane Source: MGI
- integral component of plasma membrane Source: UniProtKB
- plasma membrane Source: MGI
Other locations
- cell surface Source: MGI
- intercalated disc Source: MGI
- membrane Source: UniProtKB
- Z disc Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 422 | CytoplasmicBy similarityAdd BLAST | 422 | |
Transmembranei | 423 – 446 | Helical; Name=1By similarityAdd BLAST | 24 | |
Topological domaini | 447 – 454 | ExtracellularBy similarity | 8 | |
Transmembranei | 455 – 475 | Helical; Name=2By similarityAdd BLAST | 21 | |
Topological domaini | 476 – 478 | CytoplasmicBy similarity | 3 | |
Transmembranei | 479 – 495 | Discontinuously helical; Name=3By similarityAdd BLAST | 17 | |
Topological domaini | 496 – 504 | ExtracellularBy similarity | 9 | |
Transmembranei | 505 – 525 | Helical; Name=4By similarityAdd BLAST | 21 | |
Topological domaini | 526 – 537 | CytoplasmicBy similarityAdd BLAST | 12 | |
Transmembranei | 538 – 560 | Helical; Name=5By similarityAdd BLAST | 23 | |
Topological domaini | 561 – 588 | ExtracellularBy similarityAdd BLAST | 28 | |
Transmembranei | 589 – 609 | Helical; Name=6By similarityAdd BLAST | 21 | |
Topological domaini | 610 – 620 | CytoplasmicBy similarityAdd BLAST | 11 | |
Transmembranei | 621 – 641 | Helical; Name=7By similarityAdd BLAST | 21 | |
Topological domaini | 642 – 681 | ExtracellularBy similarityAdd BLAST | 40 | |
Transmembranei | 682 – 702 | Helical; Name=8By similarityAdd BLAST | 21 | |
Topological domaini | 703 – 718 | CytoplasmicBy similarityAdd BLAST | 16 | |
Transmembranei | 719 – 737 | Helical; Name=9By similarityAdd BLAST | 19 | |
Transmembranei | 738 – 755 | Discontinuously helical; Name=10By similarityAdd BLAST | 18 | |
Topological domaini | 756 – 778 | CytoplasmicBy similarityAdd BLAST | 23 | |
Transmembranei | 779 – 799 | Helical; Name=11By similarityAdd BLAST | 21 | |
Transmembranei | 800 – 818 | Helical; Name=12By similarityAdd BLAST | 19 | |
Topological domaini | 819 – 856 | CytoplasmicBy similarityAdd BLAST | 38 | |
Intramembranei | 857 – 887 | Discontinuously helicalBy similarityAdd BLAST | 31 | |
Topological domaini | 888 – 929 | CytoplasmicBy similarityAdd BLAST | 42 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Chemistry databases
ChEMBLi | CHEMBL4523166 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000079210 | 1 – 929 | Band 3 anion transport proteinAdd BLAST | 929 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 18 | PhosphoserineCombined sources | 1 | |
Modified residuei | 31 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 56 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 199 | PhosphoserineBy similarity | 1 | |
Modified residuei | 222 | PhosphoserineBy similarity | 1 | |
Modified residuei | 363 | PhosphoserineCombined sources | 1 | |
Modified residuei | 372 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 374 | PhosphothreonineCombined sources | 1 | |
Glycosylationi | 660 | N-linked (GlcNAc...) asparagineCurated | 1 | |
Lipidationi | 861 | S-palmitoyl cysteineBy similarity | 1 | |
Modified residuei | 922 | PhosphotyrosineBy similarity | 1 |
Keywords - PTMi
Acetylation, Glycoprotein, Lipoprotein, Palmitate, PhosphoproteinProteomic databases
CPTACi | non-CPTAC-3692 |
jPOSTi | P04919 |
MaxQBi | P04919 |
PaxDbi | P04919 |
PeptideAtlasi | P04919 |
PRIDEi | P04919 |
ProteomicsDBi | 265187 [P04919-1] 265188 [P04919-2] |
PTM databases
GlyGeni | P04919, 1 site |
iPTMneti | P04919 |
PhosphoSitePlusi | P04919 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000006574, Expressed in blood and 248 other tissues |
ExpressionAtlasi | P04919, baseline and differential |
Genevisiblei | P04919, MM |
Interactioni
Subunit structurei
A dimer in solution, but in its membrane environment, it exists primarily as a mixture of dimers and tetramers and spans the membrane asymmetrically.
Interacts (via cytoplasmic N-terminal domain) with ANK1 (via N-terminal ANK repeats); tetramer formation is critical for ankyrin association.
Interacts with STOM.
Isoform 2 interacts with TMEM139.
By similarityGO - Molecular functioni
- actin binding Source: MGI
- ankyrin binding Source: MGI
- enzyme binding Source: MGI
- hemoglobin binding Source: MGI
- protein C-terminus binding Source: MGI
- protein homodimerization activity Source: MGI
Protein-protein interaction databases
BioGRIDi | 203312, 3 interactors |
IntActi | P04919, 2 interactors |
STRINGi | 10090.ENSMUSP00000006749 |
Miscellaneous databases
RNActi | P04919, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 46 – 67 | DisorderedSequence analysisAdd BLAST | 22 | |
Regioni | 69 – 303 | GlobularBy similarityAdd BLAST | 235 | |
Regioni | 190 – 199 | Interaction with ANK1By similarity | 10 | |
Regioni | 317 – 370 | Dimerization armBy similarityAdd BLAST | 54 | |
Regioni | 366 – 389 | DisorderedSequence analysisAdd BLAST | 24 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1172, Eukaryota |
GeneTreei | ENSGT00940000157423 |
HOGENOMi | CLU_002289_1_0_1 |
InParanoidi | P04919 |
OMAi | VWGRPHL |
PhylomeDBi | P04919 |
TreeFami | TF313630 |
Family and domain databases
Gene3Di | 3.40.930.10, 1 hit |
InterProi | View protein in InterPro IPR001717, Anion_exchange IPR002977, Anion_exchange_1 IPR018241, Anion_exchange_CS IPR013769, Band3_cytoplasmic_dom IPR011531, HCO3_transpt-like_TM_dom IPR003020, HCO3_transpt_euk IPR016152, PTrfase/Anion_transptr |
PANTHERi | PTHR11453, PTHR11453, 1 hit |
Pfami | View protein in Pfam PF07565, Band_3_cyto, 1 hit PF00955, HCO3_cotransp, 2 hits |
PRINTSi | PR00165, ANIONEXCHNGR PR01187, ANIONEXHNGR1 PR01231, HCO3TRNSPORT |
SUPFAMi | SSF55804, SSF55804, 1 hit |
TIGRFAMsi | TIGR00834, ae, 1 hit |
PROSITEi | View protein in PROSITE PS00219, ANION_EXCHANGER_1, 1 hit PS00220, ANION_EXCHANGER_2, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGDMRDHEEV LEIPDRDSEE ELENIIGQIA YRDLTIPVTE MQDPEALPTE
60 70 80 90 100
QTATDYVPSS TSTPHPSSGQ VYVELQELMM DQRNQELQWV EAAHWIGLEE
110 120 130 140 150
NLREDGVWGR PHLSYLTFWS LLELQKVFSK GTFLLGLAET SLAGVANHLL
160 170 180 190 200
DCFIYEDQIR PQDREELLRA LLLKRSHAED LGNLEGVKPA VLTRSGGASE
210 220 230 240 250
PLLPHQPSLE TQLYCGQAEG GSEGPSTSGT LKIPPDSETT LVLVGRANFL
260 270 280 290 300
EKPVLGFVRL KEAVPLEDLV LPEPVGFLLV LLGPEAPHVD YTQLGRAAAT
310 320 330 340 350
LMTERVFRIT ASMAHNREEL LRSLESFLDC SLVLPPTDAP SEKALLNLVP
360 370 380 390 400
VQKELLRRRY LPSPAKPDPN LYNTLDLNGG KGGPGDEDDP LRRTGRIFGG
410 420 430 440 450
LIRDIRRRYP YYLSDITDAL SPQVLAAVIF IYFAALSPAV TFGGLLGEKT
460 470 480 490 500
RNLMGVSELL ISTAVQGILF ALLGAQPLLV LGFSGPLLVF EEAFFSFCES
510 520 530 540 550
NNLEYIVGRA WIGFWLILLV MLVVAFEGSF LVQYISRYTQ EIFSFLISLI
560 570 580 590 600
FIYETFSKLI KIFQDYPLQQ TYAPVVMKPK PQGPVPNTAL FSLVLMAGTF
610 620 630 640 650
LLAMTLRKFK NSTYFPGKLR RVIGDFGVPI SILIMVLVDS FIKGTYTQKL
660 670 680 690 700
SVPDGLKVSN SSARGWVIHP LGLYRLFPTW MMFASVLPAL LVFILIFLES
710 720 730 740 750
QITTLIVSKP ERKMIKGSGF HLDLLLVVGM GGVAALFGMP WLSATTVRSV
760 770 780 790 800
THANALTVMG KASGPGAAAQ IQEVKEQRIS GLLVSVLVGL SILMEPILSR
810 820 830 840 850
IPLAVLFGIF LYMGVTSLSG IQLFDRILLL FKPPKYHPDV PFVKRVKTWR
860 870 880 890 900
MHLFTGIQII CLAVLWVVKS TPASLALPFV LILTVPLRRL ILPLIFRELE
910 920
LQCLDGDDAK VTFDEENGLD EYDEVPMPV
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 467 | G → S in CAA27555 (PubMed:3015590).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_000454 | 1 – 79 | Missing in isoform 2. CuratedAdd BLAST | 79 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X02677 mRNA Translation: CAA26506.1 M29379 mRNA Translation: AAA37187.1 J02756 Genomic DNA Translation: AAA37278.1 BC052419 mRNA Translation: AAH52419.1 BC053429 mRNA Translation: AAH53429.1 X03917 mRNA Translation: CAA27555.1 |
CCDSi | CCDS25496.1 [P04919-1] |
PIRi | A25314 |
RefSeqi | NP_035533.1, NM_011403.2 [P04919-1] |
Genome annotation databases
Ensembli | ENSMUST00000006749; ENSMUSP00000006749; ENSMUSG00000006574 [P04919-1] |
GeneIDi | 20533 |
KEGGi | mmu:20533 |
UCSCi | uc007lrp.2, mouse [P04919-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X02677 mRNA Translation: CAA26506.1 M29379 mRNA Translation: AAA37187.1 J02756 Genomic DNA Translation: AAA37278.1 BC052419 mRNA Translation: AAH52419.1 BC053429 mRNA Translation: AAH53429.1 X03917 mRNA Translation: CAA27555.1 |
CCDSi | CCDS25496.1 [P04919-1] |
PIRi | A25314 |
RefSeqi | NP_035533.1, NM_011403.2 [P04919-1] |
3D structure databases
AlphaFoldDBi | P04919 |
SMRi | P04919 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 203312, 3 interactors |
IntActi | P04919, 2 interactors |
STRINGi | 10090.ENSMUSP00000006749 |
Chemistry databases
ChEMBLi | CHEMBL4523166 |
PTM databases
GlyGeni | P04919, 1 site |
iPTMneti | P04919 |
PhosphoSitePlusi | P04919 |
Proteomic databases
CPTACi | non-CPTAC-3692 |
jPOSTi | P04919 |
MaxQBi | P04919 |
PaxDbi | P04919 |
PeptideAtlasi | P04919 |
PRIDEi | P04919 |
ProteomicsDBi | 265187 [P04919-1] 265188 [P04919-2] |
Protocols and materials databases
Antibodypediai | 2977, 325 antibodies from 35 providers |
DNASUi | 20533 |
Genome annotation databases
Ensembli | ENSMUST00000006749; ENSMUSP00000006749; ENSMUSG00000006574 [P04919-1] |
GeneIDi | 20533 |
KEGGi | mmu:20533 |
UCSCi | uc007lrp.2, mouse [P04919-1] |
Organism-specific databases
CTDi | 6521 |
MGIi | MGI:109393, Slc4a1 |
VEuPathDBi | HostDB:ENSMUSG00000006574 |
Phylogenomic databases
eggNOGi | KOG1172, Eukaryota |
GeneTreei | ENSGT00940000157423 |
HOGENOMi | CLU_002289_1_0_1 |
InParanoidi | P04919 |
OMAi | VWGRPHL |
PhylomeDBi | P04919 |
TreeFami | TF313630 |
Enzyme and pathway databases
Reactomei | R-MMU-1237044, Erythrocytes take up carbon dioxide and release oxygen R-MMU-1247673, Erythrocytes take up oxygen and release carbon dioxide R-MMU-425381, Bicarbonate transporters |
Miscellaneous databases
BioGRID-ORCSi | 20533, 2 hits in 71 CRISPR screens |
ChiTaRSi | Slc4a1, mouse |
PROi | PR:P04919 |
RNActi | P04919, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000006574, Expressed in blood and 248 other tissues |
ExpressionAtlasi | P04919, baseline and differential |
Genevisiblei | P04919, MM |
Family and domain databases
Gene3Di | 3.40.930.10, 1 hit |
InterProi | View protein in InterPro IPR001717, Anion_exchange IPR002977, Anion_exchange_1 IPR018241, Anion_exchange_CS IPR013769, Band3_cytoplasmic_dom IPR011531, HCO3_transpt-like_TM_dom IPR003020, HCO3_transpt_euk IPR016152, PTrfase/Anion_transptr |
PANTHERi | PTHR11453, PTHR11453, 1 hit |
Pfami | View protein in Pfam PF07565, Band_3_cyto, 1 hit PF00955, HCO3_cotransp, 2 hits |
PRINTSi | PR00165, ANIONEXCHNGR PR01187, ANIONEXHNGR1 PR01231, HCO3TRNSPORT |
SUPFAMi | SSF55804, SSF55804, 1 hit |
TIGRFAMsi | TIGR00834, ae, 1 hit |
PROSITEi | View protein in PROSITE PS00219, ANION_EXCHANGER_1, 1 hit PS00220, ANION_EXCHANGER_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | B3AT_MOUSE | |
Accessioni | P04919Primary (citable) accession number: P04919 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 13, 1987 |
Last sequence update: | August 13, 1987 | |
Last modified: | May 25, 2022 | |
This is version 196 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families