UniProtKB - P04904 (GSTA3_RAT)
Protein
Glutathione S-transferase alpha-3
Gene
Gsta3
Organism
Rattus norvegicus (Rat)
Status
Functioni
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Catalyzes isomerization reactions that contribute to the biosynthesis of steroid hormones. Efficiently catalyze obligatory double-bond isomerizations of delta5-androstene-3,17-dione and delta5-pregnene-3,20-dione, precursors to testosterone and progesterone, respectively (By similarity). Has substantial activity toward aflatoxin B1-8,9-epoxide (By similarity).By similarity
Catalytic activityi
- EC:2.5.1.18By similarityThis reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 9 | GlutathioneBy similarity | 1 | |
Binding sitei | 45 | GlutathioneBy similarity | 1 |
GO - Molecular functioni
- drug binding Source: RGD
- fatty acid binding Source: RGD
- glutathione binding Source: CAFA
- glutathione peroxidase activity Source: RGD
- glutathione transferase activity Source: CAFA
- steroid delta-isomerase activity Source: RGD
GO - Biological processi
- aging Source: RGD
- epithelial cell differentiation Source: RGD
- glutathione derivative biosynthetic process Source: RGD
- glutathione metabolic process Source: RGD
- linoleic acid metabolic process Source: RGD
- prostaglandin metabolic process Source: RGD
- response to drug Source: RGD
- response to nutrient levels Source: RGD
- xenobiotic catabolic process Source: CAFA
- xenobiotic metabolic process Source: GO_Central
Keywordsi
Molecular function | Transferase |
Enzyme and pathway databases
BRENDAi | 2.5.1.18, 5301 |
Names & Taxonomyi
Protein namesi | Recommended name: Glutathione S-transferase alpha-3Curated (EC:2.5.1.18)Alternative name(s): GST 2-2 GST A3-3 GST AA Glutathione S-transferase Yc-1 Short name: GST Yc1 |
Gene namesi | |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2753, Gsta3 |
Subcellular locationi
Other locations
Cytosol
- cytosol Source: CAFA
Nucleus
- nuclear outer membrane Source: RGD
- nucleus Source: RGD
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed2 Publications | |||
ChainiPRO_0000185794 | 2 – 221 | Glutathione S-transferase alpha-3Add BLAST | 220 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 4 | N6-succinyllysineBy similarity | 1 |
Proteomic databases
PaxDbi | P04904 |
PRIDEi | P04904 |
2D gel databases
World-2DPAGEi | 0004:P04904 |
PTM databases
iPTMneti | P04904 |
PhosphoSitePlusi | P04904 |
SwissPalmi | P04904 |
Expressioni
Developmental stagei
Liver from adult female rats contains about 2-fold greater levels of YC1 than is found in liver from adult male rats.
Gene expression databases
Bgeei | ENSRNOG00000013484, Expressed in female gonad and 20 other tissues |
ExpressionAtlasi | P04904, baseline and differential |
Genevisiblei | P04904, RN |
Interactioni
Subunit structurei
Heterodimer of YC1 and YC2.
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000018325 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 3 – 83 | GST N-terminalAdd BLAST | 81 | |
Domaini | 85 – 207 | GST C-terminalAdd BLAST | 123 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 54 – 55 | Glutathione bindingBy similarity | 2 | |
Regioni | 67 – 68 | Glutathione bindingBy similarity | 2 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1695, Eukaryota |
GeneTreei | ENSGT00940000163367 |
HOGENOMi | CLU_039475_4_0_1 |
OMAi | RITNDEW |
OrthoDBi | 1162336at2759 |
PhylomeDBi | P04904 |
TreeFami | TF105321 |
Family and domain databases
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR010987, Glutathione-S-Trfase_C-like IPR036282, Glutathione-S-Trfase_C_sf IPR040079, Glutathione_S-Trfase IPR004045, Glutathione_S-Trfase_N IPR003080, GST_alpha IPR004046, GST_C IPR036249, Thioredoxin-like_sf |
Pfami | View protein in Pfam PF00043, GST_C, 1 hit PF02798, GST_N, 1 hit |
PRINTSi | PR01266, GSTRNSFRASEA |
SFLDi | SFLDS00019, Glutathione_Transferase_(cytos, 1 hit |
SUPFAMi | SSF47616, SSF47616, 1 hit SSF52833, SSF52833, 1 hit |
PROSITEi | View protein in PROSITE PS50405, GST_CTER, 1 hit PS50404, GST_NTER, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
P04904-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPGKPVLHYF DGRGRMEPIR WLLAAAGVEF EEQFLKTRDD LARLRNDGSL
60 70 80 90 100
MFQQVPMVEI DGMKLVQTRA ILNYIATKYN LYGKDMKERA LIDMYAEGVA
110 120 130 140 150
DLDEIVLHYP YIPPGEKEAS LAKIKDKARN RYFPAFEKVL KSHGQDYLVG
160 170 180 190 200
NRLSRADVYL VQVLYHVEEL DPSALANFPL LKALRTRVSN LPTVKKFLQP
210 220
GSQRKPLEDE KCVESAVKIF S
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketP00502 | GSTA1_RAT | Glutathione S-transferase alpha-1 | Gsta1 | 222 | Annotation score: | ||
P46418 | GSTA5_RAT | Glutathione S-transferase alpha-5 | Gsta5 Gstyc2 | 221 | Annotation score: | ||
A0A0G2JVT2 | A0A0G2JVT2_RAT | Glutathione transferase | LOC108348061 LOC102550391 | 145 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 102 | L → I (PubMed:2985614).Curated | 1 | |
Sequence conflicti | 184 | L → K (PubMed:2985614).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X78848 mRNA Translation: CAA55405.1 S72505 mRNA Translation: AAP21064.1 K01932 mRNA Translation: AAA41294.1 AF111160 mRNA Translation: AAD28714.1 BC059128 mRNA Translation: AAH59128.1 BC088127 mRNA Translation: AAH88127.1 |
PIRi | A26753 |
RefSeqi | NP_113697.1, NM_031509.2 XP_017452251.1, XM_017596762.1 |
Genome annotation databases
Ensembli | ENSRNOT00000018325; ENSRNOP00000018325; ENSRNOG00000013484 |
GeneIDi | 108348061 24421 |
KEGGi | rno:108348061 rno:24421 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X78848 mRNA Translation: CAA55405.1 S72505 mRNA Translation: AAP21064.1 K01932 mRNA Translation: AAA41294.1 AF111160 mRNA Translation: AAD28714.1 BC059128 mRNA Translation: AAH59128.1 BC088127 mRNA Translation: AAH88127.1 |
PIRi | A26753 |
RefSeqi | NP_113697.1, NM_031509.2 XP_017452251.1, XM_017596762.1 |
3D structure databases
SMRi | P04904 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000018325 |
Chemistry databases
ChEMBLi | CHEMBL4436 |
PTM databases
iPTMneti | P04904 |
PhosphoSitePlusi | P04904 |
SwissPalmi | P04904 |
2D gel databases
World-2DPAGEi | 0004:P04904 |
Proteomic databases
PaxDbi | P04904 |
PRIDEi | P04904 |
Genome annotation databases
Ensembli | ENSRNOT00000018325; ENSRNOP00000018325; ENSRNOG00000013484 |
GeneIDi | 108348061 24421 |
KEGGi | rno:108348061 rno:24421 |
Organism-specific databases
CTDi | 2938 |
RGDi | 2753, Gsta3 |
Phylogenomic databases
eggNOGi | KOG1695, Eukaryota |
GeneTreei | ENSGT00940000163367 |
HOGENOMi | CLU_039475_4_0_1 |
OMAi | RITNDEW |
OrthoDBi | 1162336at2759 |
PhylomeDBi | P04904 |
TreeFami | TF105321 |
Enzyme and pathway databases
BRENDAi | 2.5.1.18, 5301 |
Gene expression databases
Bgeei | ENSRNOG00000013484, Expressed in female gonad and 20 other tissues |
ExpressionAtlasi | P04904, baseline and differential |
Genevisiblei | P04904, RN |
Family and domain databases
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR010987, Glutathione-S-Trfase_C-like IPR036282, Glutathione-S-Trfase_C_sf IPR040079, Glutathione_S-Trfase IPR004045, Glutathione_S-Trfase_N IPR003080, GST_alpha IPR004046, GST_C IPR036249, Thioredoxin-like_sf |
Pfami | View protein in Pfam PF00043, GST_C, 1 hit PF02798, GST_N, 1 hit |
PRINTSi | PR01266, GSTRNSFRASEA |
SFLDi | SFLDS00019, Glutathione_Transferase_(cytos, 1 hit |
SUPFAMi | SSF47616, SSF47616, 1 hit SSF52833, SSF52833, 1 hit |
PROSITEi | View protein in PROSITE PS50405, GST_CTER, 1 hit PS50404, GST_NTER, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GSTA3_RAT | |
Accessioni | P04904Primary (citable) accession number: P04904 Secondary accession number(s): Q6LD92 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 13, 1987 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 171 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families