UniProtKB - P04899 (GNAI2_HUMAN)
Guanine nucleotide-binding protein G(i) subunit alpha-2
GNAI2
Functioni
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 47 | MagnesiumBy similarity | 1 | |
Metal bindingi | 182 | MagnesiumBy similarity | 1 | |
Binding sitei | 327 | GTP; via amide nitrogenBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 40 – 47 | GTPBy similarity | 8 | |
Nucleotide bindingi | 176 – 182 | GTPBy similarity | 7 | |
Nucleotide bindingi | 201 – 205 | GTPBy similarity | 5 | |
Nucleotide bindingi | 270 – 273 | GTPBy similarity | 4 |
GO - Molecular functioni
- G-protein beta/gamma-subunit complex binding Source: GO_Central
- G protein-coupled receptor binding Source: GO_Central
- GTPase activity Source: GO_Central
- GTP binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- adenylate cyclase-activating G protein-coupled receptor signaling pathway Source: Ensembl
- adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway Source: GO_Central
- adenylate cyclase-modulating G protein-coupled receptor signaling pathway Source: GO_Central
- cell cycle Source: UniProtKB-KW
- cell division Source: UniProtKB
- cell population proliferation Source: Ensembl
- gamma-aminobutyric acid signaling pathway Source: GO_Central
- G protein-coupled acetylcholine receptor signaling pathway Source: Ensembl
- G protein-coupled adenosine receptor signaling pathway Source: GO_Central
- G protein-coupled receptor signaling pathway Source: Reactome
- negative regulation of adenylate cyclase-activating adrenergic receptor signaling pathway involved in heart process Source: Ensembl
- negative regulation of adenylate cyclase activity Source: ProtInc
- negative regulation of apoptotic signaling pathway Source: Ensembl
- negative regulation of calcium ion-dependent exocytosis Source: Ensembl
- negative regulation of protein tyrosine phosphatase activity Source: Ensembl
- negative regulation of synaptic transmission Source: Ensembl
- positive regulation of cell migration Source: Ensembl
- positive regulation of cell population proliferation Source: BHF-UCL
- positive regulation of ERK1 and ERK2 cascade Source: Ensembl
- positive regulation of insulin receptor signaling pathway Source: Ensembl
- positive regulation of NAD(P)H oxidase activity Source: Ensembl
- positive regulation of neural precursor cell proliferation Source: Ensembl
- positive regulation of renal sodium excretion Source: Ensembl
- positive regulation of superoxide anion generation Source: Ensembl
- positive regulation of urine volume Source: Ensembl
- positive regulation of vascular associated smooth muscle cell proliferation Source: Ensembl
- protein folding Source: Reactome
- regulation of calcium ion transport Source: Ensembl
- response to nutrient Source: ProtInc
- signal transduction Source: ProtInc
Keywordsi
Molecular function | Transducer |
Biological process | Cell cycle, Cell division |
Ligand | GTP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | P04899 |
Reactomei | R-HSA-112043, PLC beta mediated events R-HSA-170670, Adenylate cyclase inhibitory pathway R-HSA-202040, G-protein activation R-HSA-392170, ADP signalling through P2Y purinoceptor 12 R-HSA-400042, Adrenaline,noradrenaline inhibits insulin secretion R-HSA-418555, G alpha (s) signalling events R-HSA-418594, G alpha (i) signalling events R-HSA-418597, G alpha (z) signalling events R-HSA-422356, Regulation of insulin secretion R-HSA-6814122, Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding R-HSA-9009391, Extra-nuclear estrogen signaling R-HSA-9660821, ADORA2B mediated anti-inflammatory cytokines production |
SignaLinki | P04899 |
SIGNORi | P04899 |
Names & Taxonomyi
Protein namesi | Recommended name: Guanine nucleotide-binding protein G(i) subunit alpha-2Alternative name(s): Adenylate cyclase-inhibiting G alpha protein |
Gene namesi | Name:GNAI2 Synonyms:GNAI2B |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:4385, GNAI2 |
MIMi | 139360, gene |
neXtProti | NX_P04899 |
VEuPathDBi | HostDB:ENSG00000114353.16 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication
Cytoskeleton
- centrosome 1 Publication
Other locations
- Cytoplasm 1 Publication
- Membrane Curated; Lipid-anchor Curated
Note: Localizes in the centrosomes of interphase and mitotic cells. Detected at the cleavage furrow and/or the midbody.
Cytoskeleton
- centrosome Source: UniProtKB
Cytosol
- cytosol Source: HPA
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
- extracellular vesicle Source: UniProtKB
Nucleus
- nucleoplasm Source: HPA
Plasma Membrane
- heterotrimeric G-protein complex Source: GO_Central
- plasma membrane Source: UniProtKB
Other locations
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Cytoskeleton, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 2771 |
MalaCardsi | GNAI2 |
OpenTargetsi | ENSG00000114353 |
PharmGKBi | PA24347 |
Miscellaneous databases
Pharosi | P04899, Tbio |
Chemistry databases
ChEMBLi | CHEMBL4105887 |
Genetic variation databases
BioMutai | GNAI2 |
DMDMi | 121023 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources3 Publications | |||
ChainiPRO_0000203679 | 2 – 355 | Guanine nucleotide-binding protein G(i) subunit alpha-2Add BLAST | 354 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 2 | N-myristoyl glycine3 Publications | 1 | |
Lipidationi | 3 | S-palmitoyl cysteineBy similarity | 1 | |
Modified residuei | 179 | ADP-ribosylarginine; by cholera toxinBy similarity | 1 | |
Modified residuei | 205 | Deamidated glutamine; by Photorhabdus PAU_022301 Publication | 1 | |
Modified residuei | 352 | ADP-ribosylcysteine; by pertussis toxinBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
ADP-ribosylation, Lipoprotein, Myristate, PalmitateProteomic databases
CPTACi | CPTAC-1530 CPTAC-1531 |
EPDi | P04899 |
jPOSTi | P04899 |
MassIVEi | P04899 |
MaxQBi | P04899 |
PaxDbi | P04899 |
PeptideAtlasi | P04899 |
PRIDEi | P04899 |
ProteomicsDBi | 3695 51752 [P04899-1] 51753 [P04899-2] 51754 [P04899-3] 51755 [P04899-4] 5863 |
PTM databases
GlyGeni | P04899, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | P04899 |
MetOSitei | P04899 |
PhosphoSitePlusi | P04899 |
SwissPalmi | P04899 |
Expressioni
Gene expression databases
Bgeei | ENSG00000114353, Expressed in blood and 238 other tissues |
ExpressionAtlasi | P04899, baseline and differential |
Genevisiblei | P04899, HS |
Organism-specific databases
HPAi | ENSG00000114353, Low tissue specificity |
Interactioni
Subunit structurei
G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.
Interacts with GPSM1 (By similarity).
Interacts with RGS12 and RGS14 (By similarity).
Interacts with UNC5B (PubMed:12359238).
Interacts (inactive GDP-bound form) with NUCB1 (via GBA motif); the interaction leads to activation of GNAI3 (By similarity).
Interacts (inactive GDP-bound form) with CCDC88C/DAPLE (via GBA motif) (PubMed:26126266).
Interacts (inactive GDP-bound form) with CCDC8A/GIV (via GBA motif) (PubMed:19211784).
By similarity3 PublicationsBinary interactionsi
Hide detailsP04899
GO - Molecular functioni
- G protein-coupled receptor binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 109033, 162 interactors |
CORUMi | P04899 |
DIPi | DIP-602N |
IntActi | P04899, 78 interactors |
MINTi | P04899 |
STRINGi | 9606.ENSP00000312999 |
Miscellaneous databases
RNActi | P04899, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 32 – 355 | G-alphaPROSITE-ProRule annotationAdd BLAST | 324 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 35 – 48 | G1 motifPROSITE-ProRule annotationAdd BLAST | 14 | |
Regioni | 174 – 182 | G2 motifPROSITE-ProRule annotation | 9 | |
Regioni | 197 – 206 | G3 motifPROSITE-ProRule annotation | 10 | |
Regioni | 266 – 273 | G4 motifPROSITE-ProRule annotation | 8 | |
Regioni | 325 – 330 | G5 motifPROSITE-ProRule annotation | 6 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0082, Eukaryota |
GeneTreei | ENSGT00940000155125 |
HOGENOMi | CLU_014184_6_0_1 |
InParanoidi | P04899 |
OMAi | PNKYEEA |
PhylomeDBi | P04899 |
TreeFami | TF300673 |
Family and domain databases
CDDi | cd00066, G-alpha, 1 hit |
Gene3Di | 1.10.400.10, 1 hit |
InterProi | View protein in InterPro IPR001408, Gprotein_alpha_I IPR001019, Gprotein_alpha_su IPR011025, GproteinA_insert IPR027417, P-loop_NTPase |
PANTHERi | PTHR10218, PTHR10218, 1 hit |
Pfami | View protein in Pfam PF00503, G-alpha, 1 hit |
PRINTSi | PR00318, GPROTEINA PR00441, GPROTEINAI |
SMARTi | View protein in SMART SM00275, G_alpha, 1 hit |
SUPFAMi | SSF47895, SSF47895, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51882, G_ALPHA, 1 hit |
s (6+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 6 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 6 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGCTVSAEDK AAAERSKMID KNLREDGEKA AREVKLLLLG AGESGKSTIV
60 70 80 90 100
KQMKIIHEDG YSEEECRQYR AVVYSNTIQS IMAIVKAMGN LQIDFADPSR
110 120 130 140 150
ADDARQLFAL SCTAEEQGVL PDDLSGVIRR LWADHGVQAC FGRSREYQLN
160 170 180 190 200
DSAAYYLNDL ERIAQSDYIP TQQDVLRTRV KTTGIVETHF TFKDLHFKMF
210 220 230 240 250
DVGGQRSERK KWIHCFEGVT AIIFCVALSA YDLVLAEDEE MNRMHESMKL
260 270 280 290 300
FDSICNNKWF TDTSIILFLN KKDLFEEKIT HSPLTICFPE YTGANKYDEA
310 320 330 340 350
ASYIQSKFED LNKRKDTKEI YTHFTCATDT KNVQFVFDAV TDVIIKNNLK
DCGLF
The sequence of this isoform differs from the canonical sequence as follows:
332-355: NVQFVFDAVTDVIIKNNLKDCGLF → SRKLFRETYLKLSGPDQHPHPSPAPAPPLSSDSVP
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF8WE78 | F8WE78_HUMAN | Guanine nucleotide-binding protein ... | GNAI2 | 142 | Annotation score: | ||
F8WBG4 | F8WBG4_HUMAN | Guanine nucleotide-binding protein ... | GNAI2 | 146 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_054788 | 1 – 52 | Missing in isoform 6. 1 PublicationAdd BLAST | 52 | |
Alternative sequenceiVSP_017497 | 1 – 39 | MGCTV…KLLLL → MEYAGHLPASSAQGTILACT SCT in isoform 2. 1 PublicationAdd BLAST | 39 | |
Alternative sequenceiVSP_043473 | 1 – 39 | MGCTV…KLLLL → MR in isoform 3. 1 PublicationAdd BLAST | 39 | |
Alternative sequenceiVSP_054789 | 1 – 39 | MGCTV…KLLLL → MTEGVKTLGWTKQKGGCHWG RSE in isoform 5. 1 PublicationAdd BLAST | 39 | |
Alternative sequenceiVSP_043903 | 332 – 355 | NVQFV…DCGLF → SRKLFRETYLKLSGPDQHPH PSPAPAPPLSSDSVP in isoform sGi2. 1 PublicationAdd BLAST | 24 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X04828 mRNA Translation: CAA28512.1 J03004 mRNA Translation: AAA52556.1 Sequence problems. M20593 , M20586, M20587, M20588, M20589, M20590, M20591, M20592 Genomic DNA Translation: AAA35894.1 AY677118 mRNA Translation: AAT78421.1 AF493906 mRNA Translation: AAM12620.1 AK096299 mRNA Translation: BAG53252.1 AK096595 mRNA Translation: BAG53334.1 AK302330 mRNA Translation: BAG63662.1 AK304473 mRNA Translation: BAG65287.1 AC002077 Genomic DNA No translation available. U73166 Genomic DNA No translation available. U73169 Genomic DNA No translation available. CH471055 Genomic DNA Translation: EAW65053.1 CH471055 Genomic DNA Translation: EAW65055.1 CH471055 Genomic DNA Translation: EAW65056.1 BC012138 mRNA Translation: AAH12138.1 BC016995 mRNA Translation: AAH16995.1 X07854 Genomic DNA Translation: CAA30703.1 |
CCDSi | CCDS2813.1 [P04899-1] CCDS54587.1 [P04899-3] CCDS63642.1 [P04899-5] CCDS63644.1 [P04899-6] |
PIRi | S02319, RGHUI2 |
RefSeqi | NP_001159897.1, NM_001166425.1 [P04899-3] NP_001269546.1, NM_001282617.1 [P04899-6] NP_001269547.1, NM_001282618.1 NP_001269548.1, NM_001282619.1 [P04899-2] NP_001269549.1, NM_001282620.1 [P04899-5] NP_002061.1, NM_002070.3 [P04899-1] |
Genome annotation databases
Ensembli | ENST00000266027; ENSP00000266027; ENSG00000114353 [P04899-6] ENST00000313601; ENSP00000312999; ENSG00000114353 [P04899-1] ENST00000422163; ENSP00000406871; ENSG00000114353 [P04899-5] ENST00000440628; ENSP00000395736; ENSG00000114353 [P04899-6] ENST00000451956; ENSP00000406369; ENSG00000114353 [P04899-3] |
GeneIDi | 2771 |
KEGGi | hsa:2771 |
UCSCi | uc003cyp.3, human [P04899-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6D9H | electron microscopy | 3.60 | A | 1-355 | [»] | |
SMRi | P04899 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 109033, 162 interactors |
CORUMi | P04899 |
DIPi | DIP-602N |
IntActi | P04899, 78 interactors |
MINTi | P04899 |
STRINGi | 9606.ENSP00000312999 |
Chemistry databases
ChEMBLi | CHEMBL4105887 |
PTM databases
GlyGeni | P04899, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | P04899 |
MetOSitei | P04899 |
PhosphoSitePlusi | P04899 |
SwissPalmi | P04899 |
Genetic variation databases
BioMutai | GNAI2 |
DMDMi | 121023 |
Proteomic databases
CPTACi | CPTAC-1530 CPTAC-1531 |
EPDi | P04899 |
jPOSTi | P04899 |
MassIVEi | P04899 |
MaxQBi | P04899 |
PaxDbi | P04899 |
PeptideAtlasi | P04899 |
PRIDEi | P04899 |
ProteomicsDBi | 3695 51752 [P04899-1] 51753 [P04899-2] 51754 [P04899-3] 51755 [P04899-4] 5863 |
Protocols and materials databases
Antibodypediai | 2180, 284 antibodies |
DNASUi | 2771 |
Genome annotation databases
Ensembli | ENST00000266027; ENSP00000266027; ENSG00000114353 [P04899-6] ENST00000313601; ENSP00000312999; ENSG00000114353 [P04899-1] ENST00000422163; ENSP00000406871; ENSG00000114353 [P04899-5] ENST00000440628; ENSP00000395736; ENSG00000114353 [P04899-6] ENST00000451956; ENSP00000406369; ENSG00000114353 [P04899-3] |
GeneIDi | 2771 |
KEGGi | hsa:2771 |
UCSCi | uc003cyp.3, human [P04899-1] |
Organism-specific databases
CTDi | 2771 |
DisGeNETi | 2771 |
GeneCardsi | GNAI2 |
HGNCi | HGNC:4385, GNAI2 |
HPAi | ENSG00000114353, Low tissue specificity |
MalaCardsi | GNAI2 |
MIMi | 139360, gene |
neXtProti | NX_P04899 |
OpenTargetsi | ENSG00000114353 |
PharmGKBi | PA24347 |
VEuPathDBi | HostDB:ENSG00000114353.16 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0082, Eukaryota |
GeneTreei | ENSGT00940000155125 |
HOGENOMi | CLU_014184_6_0_1 |
InParanoidi | P04899 |
OMAi | PNKYEEA |
PhylomeDBi | P04899 |
TreeFami | TF300673 |
Enzyme and pathway databases
PathwayCommonsi | P04899 |
Reactomei | R-HSA-112043, PLC beta mediated events R-HSA-170670, Adenylate cyclase inhibitory pathway R-HSA-202040, G-protein activation R-HSA-392170, ADP signalling through P2Y purinoceptor 12 R-HSA-400042, Adrenaline,noradrenaline inhibits insulin secretion R-HSA-418555, G alpha (s) signalling events R-HSA-418594, G alpha (i) signalling events R-HSA-418597, G alpha (z) signalling events R-HSA-422356, Regulation of insulin secretion R-HSA-6814122, Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding R-HSA-9009391, Extra-nuclear estrogen signaling R-HSA-9660821, ADORA2B mediated anti-inflammatory cytokines production |
SignaLinki | P04899 |
SIGNORi | P04899 |
Miscellaneous databases
BioGRID-ORCSi | 2771, 21 hits in 873 CRISPR screens |
ChiTaRSi | GNAI2, human |
GeneWikii | GNAI2 |
GenomeRNAii | 2771 |
Pharosi | P04899, Tbio |
PROi | PR:P04899 |
RNActi | P04899, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000114353, Expressed in blood and 238 other tissues |
ExpressionAtlasi | P04899, baseline and differential |
Genevisiblei | P04899, HS |
Family and domain databases
CDDi | cd00066, G-alpha, 1 hit |
Gene3Di | 1.10.400.10, 1 hit |
InterProi | View protein in InterPro IPR001408, Gprotein_alpha_I IPR001019, Gprotein_alpha_su IPR011025, GproteinA_insert IPR027417, P-loop_NTPase |
PANTHERi | PTHR10218, PTHR10218, 1 hit |
Pfami | View protein in Pfam PF00503, G-alpha, 1 hit |
PRINTSi | PR00318, GPROTEINA PR00441, GPROTEINAI |
SMARTi | View protein in SMART SM00275, G_alpha, 1 hit |
SUPFAMi | SSF47895, SSF47895, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51882, G_ALPHA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GNAI2_HUMAN | |
Accessioni | P04899Primary (citable) accession number: P04899 Secondary accession number(s): B3KTZ0 Q8IZ71 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 13, 1987 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 10, 2021 | |
This is version 229 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 3
Human chromosome 3: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families