GNAI2

Functionⁱ

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. May play a role in cell division.

1 Publication

Isoform sGi2:

Regulates the cell surface density of dopamine receptors DRD2 by sequestrating them as an intracellular pool.

1 Publication

Sites

Feature key	Position(s)	DescriptionActions	Length
Metal bindingⁱ	47	MagnesiumBy similarity	1
Metal bindingⁱ	182	MagnesiumBy similarity	1
Binding siteⁱ	327	GTP; via amide nitrogenBy similarity	1

Regions

Feature key	Position(s)	DescriptionActions	Length
Nucleotide bindingⁱ	40 – 47	GTPBy similarity	8
Nucleotide bindingⁱ	176 – 182	GTPBy similarity	7
Nucleotide bindingⁱ	201 – 205	GTPBy similarity	5
Nucleotide bindingⁱ	270 – 273	GTPBy similarity	4

GO - Molecular functionⁱ

Complete GO annotation on QuickGO ...

GO - Biological processⁱ

Complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Transducer
Biological process	Cell cycle, Cell division
Ligand	GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

PathwayCommonsⁱ	P04899
Reactomeⁱ	R-HSA-112043, PLC beta mediated events R-HSA-170670, Adenylate cyclase inhibitory pathway R-HSA-202040, G-protein activation R-HSA-392170, ADP signalling through P2Y purinoceptor 12 R-HSA-400042, Adrenaline,noradrenaline inhibits insulin secretion R-HSA-418555, G alpha (s) signalling events R-HSA-418594, G alpha (i) signalling events R-HSA-418597, G alpha (z) signalling events R-HSA-422356, Regulation of insulin secretion R-HSA-6814122, Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding R-HSA-9009391, Extra-nuclear estrogen signaling R-HSA-9660821, ADORA2B mediated anti-inflammatory cytokines production
SignaLinkⁱ	P04899
SIGNORⁱ	P04899

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Guanine nucleotide-binding protein G(i) subunit alpha-2 Alternative name(s): Adenylate cyclase-inhibiting G alpha protein
Gene namesⁱ	Name:GNAI2 Synonyms:GNAI2B
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 3

Organism-specific databases

Human Gene Nomenclature Database More...HGNCⁱ	HGNC:4385, GNAI2
MIMⁱ	139360, gene
neXtProtⁱ	NX_P04899
VEuPathDBⁱ	HostDB:ENSG00000114353.16

Keywords - Cellular componentⁱ

Cell membrane, Cytoplasm, Cytoskeleton, Membrane

Pathology & Biotechⁱ

Organism-specific databases

DisGeNET More...DisGeNETⁱ	2771
MalaCards human disease database More...MalaCardsⁱ	GNAI2
Open Targets More...OpenTargetsⁱ	ENSG00000114353
PharmGKBⁱ	PA24347

Miscellaneous databases

Pharosⁱ	P04899, Tbio

Pharosⁱ

P04899, Tbio

Chemistry databases

ChEMBLⁱ	CHEMBL4105887

ChEMBLⁱ

CHEMBL4105887

Genetic variation databases

BioMutaⁱ	GNAI2
DMDMⁱ	121023

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Initiator methionineⁱ		RemovedCombined sources3 Publications
ChainⁱPRO_0000203679	2 – 355	Guanine nucleotide-binding protein G(i) subunit alpha-2Add BLAST		354

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Lipidationⁱ	2	N-myristoyl glycine3 Publications	1
Lipidationⁱ	3	S-palmitoyl cysteineBy similarity	1
Modified residueⁱ	179	ADP-ribosylarginine; by cholera toxinBy similarity	1
Modified residueⁱ	205	Deamidated glutamine; by Photorhabdus PAU_022301 Publication	1
Modified residueⁱ	352	ADP-ribosylcysteine; by pertussis toxinBy similarity	1

Post-translational modificationⁱ

(Microbial infection) Deamidated at Gln-205 by Photorhabdus asymbiotica toxin PAU_02230, blocking GTP hydrolysis of heterotrimeric GNAQ or GNA11 and G-alphai (GNAI1, GNAI2 or GNAI3) proteins, thereby activating RhoA.1 Publication

Keywords - PTMⁱ

ADP-ribosylation, Lipoprotein, Myristate, Palmitate

Proteomic databases

The CPTAC Assay portal More...CPTACⁱ	CPTAC-1530 CPTAC-1531
EPDⁱ	P04899
jPOSTⁱ	P04899
MassIVEⁱ	P04899
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P04899
PaxDbⁱ	P04899
PeptideAtlas More...PeptideAtlasⁱ	P04899
PRIDEⁱ	P04899
ProteomicsDBⁱ	3695 51752 [P04899-1] 51753 [P04899-2] 51754 [P04899-3] 51755 [P04899-4] 5863

PTM databases

GlyGenⁱ	P04899, 1 site, 1 O-linked glycan (1 site)
iPTMnetⁱ	P04899
MetOSiteⁱ	P04899
PhosphoSitePlusⁱ	P04899
SwissPalmⁱ	P04899

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSG00000114353, Expressed in blood and 239 other tissues
ExpressionAtlasⁱ	P04899, baseline and differential
Genevisibleⁱ	P04899, HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	ENSG00000114353, Low tissue specificity

HPAⁱ

ENSG00000114353, Low tissue specificity

Interactionⁱ

Subunit structureⁱ

G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.

Interacts with GPSM1 (By similarity).

Interacts with RGS12 and RGS14 (By similarity).

Interacts with UNC5B (PubMed:12359238).

Interacts (inactive GDP-bound form) with NUCB1 (via GBA motif); the interaction leads to activation of GNAI3 (By similarity).

Interacts (inactive GDP-bound form) with CCDC88C/DAPLE (via GBA motif) (PubMed:26126266).

Interacts (inactive GDP-bound form) with CCDC8A/GIV (via GBA motif) (PubMed:19211784).

By similarity3 Publications

Binary interactionsⁱ

Hide details

P04899

With	#Exp.	IntAct
CXCR5 [P32302]	5	EBI-353997,EBI-2835269
GPSM3 [Q9Y4H4]	3	EBI-353997,EBI-347538
KRT31 [Q15323]	3	EBI-353997,EBI-948001
KRT40 [Q6A162]	3	EBI-353997,EBI-10171697
KRTAP10-8 [P60410]	3	EBI-353997,EBI-10171774
MDFI [Q99750]	5	EBI-353997,EBI-724076
NCF1 [P14598]	2	EBI-353997,EBI-395044
NCF2 [P19878]	4	EBI-353997,EBI-489611
NOTCH2NLA [Q7Z3S9]	3	EBI-353997,EBI-945833
PCP2 [Q8IVA1]	3	EBI-353997,EBI-12250122
RGS20 - isoform 5 [O76081-6]	3	EBI-353997,EBI-10178530
TRIP6 [Q15654]	3	EBI-353997,EBI-742327
Oprm1 [P42866] from Mus musculus.	2	EBI-353997,EBI-5282656

GO - Molecular functionⁱ

G protein-coupled receptor binding
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- "Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium."
  Gaudet P., Livstone M.S., Lewis S.E., Thomas P.D.
  Brief Bioinform 12:449-462(2011) [PubMed] [Europe PMC] [Abstract]

Complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGRIDⁱ	109033, 175 interactors
CORUMⁱ	P04899
Database of interacting proteins More...DIPⁱ	DIP-602N
IntActⁱ	P04899, 78 interactors
Molecular INTeraction database More...MINTⁱ	P04899
STRINGⁱ	9606.ENSP00000312999

Miscellaneous databases

RNActⁱ	P04899, protein

RNActⁱ

P04899, protein

Structureⁱ

3D structure databases

SMRⁱ	P04899
ModBaseⁱ	Search...
PDBe-KBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	32 – 355	G-alphaPROSITE-ProRule annotationAdd BLAST		324

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	35 – 48	G1 motifPROSITE-ProRule annotationAdd BLAST	14
Regionⁱ	174 – 182	G2 motifPROSITE-ProRule annotation	9
Regionⁱ	197 – 206	G3 motifPROSITE-ProRule annotation	10
Regionⁱ	266 – 273	G4 motifPROSITE-ProRule annotation	8
Regionⁱ	325 – 330	G5 motifPROSITE-ProRule annotation	6

Sequence similaritiesⁱ

Belongs to the G-alpha family. G(i/o/t/z) subfamily.Curated

Phylogenomic databases

eggNOGⁱ	KOG0082, Eukaryota
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00940000155125
HOGENOMⁱ	CLU_014184_6_0_1
InParanoidⁱ	P04899
OMAⁱ	PNKYEEA
PhylomeDBⁱ	P04899
TreeFamⁱ	TF300673

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00066, G-alpha, 1 hit
Gene3Dⁱ	1.10.400.10, 1 hit
InterProⁱ	View protein in InterPro IPR001408, Gprotein_alpha_I IPR001019, Gprotein_alpha_su IPR011025, GproteinA_insert IPR027417, P-loop_NTPase
PANTHERⁱ	PTHR10218, PTHR10218, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00503, G-alpha, 1 hit
PRINTSⁱ	PR00318, GPROTEINA PR00441, GPROTEINAI
SMARTⁱ	View protein in SMART SM00275, G_alpha, 1 hit
SUPFAMⁱ	SSF47895, SSF47895, 1 hit SSF52540, SSF52540, 1 hit
PROSITEⁱ	View protein in PROSITE PS51882, G_ALPHA, 1 hit

Sequences (6+)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry describes 6 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 6 described isoforms and 2 potential isoforms that are computationally mapped.Show all Align All

Isoform 1 (identifier: P04899-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the canonicalⁱ sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGCTVSAEDK AAAERSKMID KNLREDGEKA AREVKLLLLG AGESGKSTIV 
        60         70         80         90        100
KQMKIIHEDG YSEEECRQYR AVVYSNTIQS IMAIVKAMGN LQIDFADPSR 
       110        120        130        140        150
ADDARQLFAL SCTAEEQGVL PDDLSGVIRR LWADHGVQAC FGRSREYQLN 
       160        170        180        190        200
DSAAYYLNDL ERIAQSDYIP TQQDVLRTRV KTTGIVETHF TFKDLHFKMF 
       210        220        230        240        250
DVGGQRSERK KWIHCFEGVT AIIFCVALSA YDLVLAEDEE MNRMHESMKL 
       260        270        280        290        300
FDSICNNKWF TDTSIILFLN KKDLFEEKIT HSPLTICFPE YTGANKYDEA 
       310        320        330        340        350
ASYIQSKFED LNKRKDTKEI YTHFTCATDT KNVQFVFDAV TDVIIKNNLK 

DCGLF

Length:355

Mass (Da):40,451

Last modified:January 23, 2007 - v3

Checksum:ⁱBE6D6FF720CF3DCC

GO

Isoform 2 (identifier: P04899-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
1-39: MGCTVSAEDKAAAERSKMIDKNLREDGEKAAREVKLLLL → MEYAGHLPASSAQGTILACTSCT

« Hide

        10         20         30         40         50
MEYAGHLPAS SAQGTILACT SCTGAGESGK STIVKQMKII HEDGYSEEEC 
        60         70         80         90        100
RQYRAVVYSN TIQSIMAIVK AMGNLQIDFA DPSRADDARQ LFALSCTAEE 
       110        120        130        140        150
QGVLPDDLSG VIRRLWADHG VQACFGRSRE YQLNDSAAYY LNDLERIAQS 
       160        170        180        190        200
DYIPTQQDVL RTRVKTTGIV ETHFTFKDLH FKMFDVGGQR SERKKWIHCF 
       210        220        230        240        250
EGVTAIIFCV ALSAYDLVLA EDEEMNRMHE SMKLFDSICN NKWFTDTSII 
       260        270        280        290        300
LFLNKKDLFE EKITHSPLTI CFPEYTGANK YDEAASYIQS KFEDLNKRKD 
       310        320        330 
TKEIYTHFTC ATDTKNVQFV FDAVTDVIIK NNLKDCGLF

Show »

Length:339

Mass (Da):38,473

Checksum:ⁱF14AB734881535C5

GO

Isoform 3 (identifier: P04899-3) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
1-39: MGCTVSAEDKAAAERSKMIDKNLREDGEKAAREVKLLLL → MR

« Hide

        10         20         30         40         50
MRGAGESGKS TIVKQMKIIH EDGYSEEECR QYRAVVYSNT IQSIMAIVKA 
        60         70         80         90        100
MGNLQIDFAD PSRADDARQL FALSCTAEEQ GVLPDDLSGV IRRLWADHGV 
       110        120        130        140        150
QACFGRSREY QLNDSAAYYL NDLERIAQSD YIPTQQDVLR TRVKTTGIVE 
       160        170        180        190        200
THFTFKDLHF KMFDVGGQRS ERKKWIHCFE GVTAIIFCVA LSAYDLVLAE 
       210        220        230        240        250
DEEMNRMHES MKLFDSICNN KWFTDTSIIL FLNKKDLFEE KITHSPLTIC 
       260        270        280        290        300
FPEYTGANKY DEAASYIQSK FEDLNKRKDT KEIYTHFTCA TDTKNVQFVF 
       310 
DAVTDVIIKN NLKDCGLF

Show »

Length:318

Mass (Da):36,465

Checksum:ⁱA0131D7E46EC5370

GO

Isoform sGi2 (identifier: P04899-4) [UniParc]FASTA Add to basket

Also known as: sGalphai2

The sequence of this isoform differs from the canonical sequence as follows:
332-355: NVQFVFDAVTDVIIKNNLKDCGLF → SRKLFRETYLKLSGPDQHPHPSPAPAPPLSSDSVP

« Hide

        10         20         30         40         50
MGCTVSAEDK AAAERSKMID KNLREDGEKA AREVKLLLLG AGESGKSTIV 
        60         70         80         90        100
KQMKIIHEDG YSEEECRQYR AVVYSNTIQS IMAIVKAMGN LQIDFADPSR 
       110        120        130        140        150
ADDARQLFAL SCTAEEQGVL PDDLSGVIRR LWADHGVQAC FGRSREYQLN 
       160        170        180        190        200
DSAAYYLNDL ERIAQSDYIP TQQDVLRTRV KTTGIVETHF TFKDLHFKMF 
       210        220        230        240        250
DVGGQRSERK KWIHCFEGVT AIIFCVALSA YDLVLAEDEE MNRMHESMKL 
       260        270        280        290        300
FDSICNNKWF TDTSIILFLN KKDLFEEKIT HSPLTICFPE YTGANKYDEA 
       310        320        330        340        350
ASYIQSKFED LNKRKDTKEI YTHFTCATDT KSRKLFRETY LKLSGPDQHP 
       360 
HPSPAPAPPL SSDSVP

Show »

Length:366

Mass (Da):41,548

Checksum:ⁱEF7FED14C8A0A8B4

GO

Isoform 5 (identifier: P04899-5) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
1-39: MGCTVSAEDKAAAERSKMIDKNLREDGEKAAREVKLLLL → MTEGVKTLGWTKQKGGCHWGRSE

« Hide

        10         20         30         40         50
MTEGVKTLGW TKQKGGCHWG RSEGAGESGK STIVKQMKII HEDGYSEEEC 
        60         70         80         90        100
RQYRAVVYSN TIQSIMAIVK AMGNLQIDFA DPSRADDARQ LFALSCTAEE 
       110        120        130        140        150
QGVLPDDLSG VIRRLWADHG VQACFGRSRE YQLNDSAAYY LNDLERIAQS 
       160        170        180        190        200
DYIPTQQDVL RTRVKTTGIV ETHFTFKDLH FKMFDVGGQR SERKKWIHCF 
       210        220        230        240        250
EGVTAIIFCV ALSAYDLVLA EDEEMNRMHE SMKLFDSICN NKWFTDTSII 
       260        270        280        290        300
LFLNKKDLFE EKITHSPLTI CFPEYTGANK YDEAASYIQS KFEDLNKRKD 
       310        320        330 
TKEIYTHFTC ATDTKNVQFV FDAVTDVIIK NNLKDCGLF

Show »

Length:339

Mass (Da):38,737

Checksum:ⁱBFCDD8678696698F

GO

Isoform 6 (identifier: P04899-6) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
1-52: Missing.

« Hide

        10         20         30         40         50
MKIIHEDGYS EEECRQYRAV VYSNTIQSIM AIVKAMGNLQ IDFADPSRAD 
        60         70         80         90        100
DARQLFALSC TAEEQGVLPD DLSGVIRRLW ADHGVQACFG RSREYQLNDS 
       110        120        130        140        150
AAYYLNDLER IAQSDYIPTQ QDVLRTRVKT TGIVETHFTF KDLHFKMFDV 
       160        170        180        190        200
GGQRSERKKW IHCFEGVTAI IFCVALSAYD LVLAEDEEMN RMHESMKLFD 
       210        220        230        240        250
SICNNKWFTD TSIILFLNKK DLFEEKITHS PLTICFPEYT GANKYDEAAS 
       260        270        280        290        300
YIQSKFEDLN KRKDTKEIYT HFTCATDTKN VQFVFDAVTD VIIKNNLKDC 

GLF

Show »

Length:303

Mass (Da):34,935

Checksum:ⁱ9E676EE9177E166C

GO

Computationally mapped potential isoform sequencesⁱ

There are 2 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

F8WE78	F8WE78_HUMAN	Guanine nucleotide-binding protein ... Guanine nucleotide-binding protein G(i) subunit alpha-2	GNAI2	142	Annotation score:
F8WBG4	F8WBG4_HUMAN	Guanine nucleotide-binding protein ... Guanine nucleotide-binding protein G(i) subunit alpha-2	GNAI2	146	Annotation score:

Alternative sequence

Feature key	Position(s)	DescriptionActions	Length
Alternative sequenceⁱVSP_054788	1 – 52	Missing in isoform 6. 1 PublicationAdd BLAST	52
Alternative sequenceⁱVSP_017497	1 – 39	MGCTV…KLLLL → MEYAGHLPASSAQGTILACT SCT in isoform 2. 1 PublicationAdd BLAST	39
Alternative sequenceⁱVSP_043473	1 – 39	MGCTV…KLLLL → MR in isoform 3. 1 PublicationAdd BLAST	39
Alternative sequenceⁱVSP_054789	1 – 39	MGCTV…KLLLL → MTEGVKTLGWTKQKGGCHWG RSE in isoform 5. 1 PublicationAdd BLAST	39
Alternative sequenceⁱVSP_043903	332 – 355	NVQFV…DCGLF → SRKLFRETYLKLSGPDQHPH PSPAPAPPLSSDSVP in isoform sGi2. 1 PublicationAdd BLAST	24

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X04828 mRNA Translation: CAA28512.1 J03004 mRNA Translation: AAA52556.1 Sequence problems. M20593 M20592 Genomic DNA Translation: AAA35894.1 AY677118 mRNA Translation: AAT78421.1 AF493906 mRNA Translation: AAM12620.1 AK096299 mRNA Translation: BAG53252.1 AK096595 mRNA Translation: BAG53334.1 AK302330 mRNA Translation: BAG63662.1 AK304473 mRNA Translation: BAG65287.1 AC002077 Genomic DNA No translation available. U73166 Genomic DNA No translation available. U73169 Genomic DNA No translation available. CH471055 Genomic DNA Translation: EAW65053.1 CH471055 Genomic DNA Translation: EAW65055.1 CH471055 Genomic DNA Translation: EAW65056.1 BC012138 mRNA Translation: AAH12138.1 BC016995 mRNA Translation: AAH16995.1 X07854 Genomic DNA Translation: CAA30703.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS2813.1 [P04899-1] CCDS54587.1 [P04899-3] CCDS63642.1 [P04899-5] CCDS63644.1 [P04899-6]
PIRⁱ	S02319, RGHUI2
NCBI Reference Sequences More...RefSeqⁱ	NP_001159897.1, NM_001166425.1 [P04899-3] NP_001269546.1, NM_001282617.1 [P04899-6] NP_001269547.1, NM_001282618.1 NP_001269548.1, NM_001282619.1 [P04899-2] NP_001269549.1, NM_001282620.1 [P04899-5] NP_002061.1, NM_002070.3 [P04899-1]

Genome annotation databases

Ensemblⁱ	ENST00000266027; ENSP00000266027; ENSG00000114353 [P04899-6] ENST00000313601; ENSP00000312999; ENSG00000114353 [P04899-1] ENST00000422163; ENSP00000406871; ENSG00000114353 [P04899-5] ENST00000440628; ENSP00000395736; ENSG00000114353 [P04899-6] ENST00000451956; ENSP00000406369; ENSG00000114353 [P04899-3]
GeneIDⁱ	2771
KEGGⁱ	hsa:2771
UCSC genome browser More...UCSCⁱ	uc003cyp.3, human [P04899-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P04899	G protein subunit alpha i2		MANLE		350	UniRef100_P04899
Guanine nucleotide-binding protein G(I) subunit alpha-2 isoform 1 (Fragment)		MACMU		350
guanine nucleotide-binding protein G(I) subunit alpha-2 isoform X1		CARSF		355
G protein subunit alpha i2		PANTR		355
Uncharacterized protein		RHIRO		355
+27
P04899-3	GNAI2 isoform 9		PONAB		318	UniRef100_A0A2J8TH72
Uncharacterized protein		RHIRO		318
GNAI2 isoform 9		PANTR		318
Uncharacterized protein		NOMLE		318
Uncharacterized protein		RHIBE		318
+2

Protein	Similar proteins		Species	Score	Length	Source
P04899	Guanine nucleotide-binding protein G(i) subunit alpha-2		MOUSE		355	UniRef90_P04899
Guanine nucleotide-binding protein G(i) subunit alpha-2		MACFA		355
Guanine nucleotide-binding protein G(i) subunit alpha-2		RAT		355
Guanine nucleotide-binding protein G(i) subunit alpha-2		CANLF		355
G protein subunit alpha i2		MANLE		350
+153
P04899-2	G protein subunit alpha i2		URSMA		408	UniRef90_P04899-2
guanine nucleotide-binding protein G(I) subunit alpha-2 isoform X2		ODORO		345
G protein subunit alpha i2		PAPAN		339
Uncharacterized protein		RHIRO		339
G protein subunit alpha i2		MANLE		339
+13
P04899-3	Isoform sGi2 of Guanine nucleotide-binding protein G(i) subunit alpha-2		HUMAN		366	UniRef90_P04899-4
Uncharacterized protein		CALJA		348
GNAI2 isoform 9		PONAB		318
Uncharacterized protein		RHIRO		318
GNAI2 isoform 9		PANTR		318
+19

Protein	Similar proteins		Species	Score	Length	Source
P04899	Guanine nucleotide-binding protein G(i) subunit alpha-2		MOUSE		355	UniRef50_P04899
Guanine nucleotide-binding protein G(i) subunit alpha-2		MACFA		355
Guanine nucleotide-binding protein G(i) subunit alpha-2		RAT		355
Guanine nucleotide-binding protein G(i) subunit alpha-2		CANLF		355
Guanine nucleotide-binding protein G(i) subunit alpha-2		CAVPO		355
+1416
P04899-3	Isoform sGi2 of Guanine nucleotide-binding protein G(i) subunit alpha-2		HUMAN		366	UniRef50_P04899-4
Guanine nucleotide-binding protein G(i) subunit alpha		HOMAM		355
Uncharacterized protein		CALJA		348
G protein subunit alpha i2		PANPA		318
G protein subunit alpha i2		GORGO		318
+132

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X04828 mRNA Translation: CAA28512.1 J03004 mRNA Translation: AAA52556.1 Sequence problems. M20593 M20592 Genomic DNA Translation: AAA35894.1 AY677118 mRNA Translation: AAT78421.1 AF493906 mRNA Translation: AAM12620.1 AK096299 mRNA Translation: BAG53252.1 AK096595 mRNA Translation: BAG53334.1 AK302330 mRNA Translation: BAG63662.1 AK304473 mRNA Translation: BAG65287.1 AC002077 Genomic DNA No translation available. U73166 Genomic DNA No translation available. U73169 Genomic DNA No translation available. CH471055 Genomic DNA Translation: EAW65053.1 CH471055 Genomic DNA Translation: EAW65055.1 CH471055 Genomic DNA Translation: EAW65056.1 BC012138 mRNA Translation: AAH12138.1 BC016995 mRNA Translation: AAH16995.1 X07854 Genomic DNA Translation: CAA30703.1
CCDSⁱ	CCDS2813.1 [P04899-1] CCDS54587.1 [P04899-3] CCDS63642.1 [P04899-5] CCDS63644.1 [P04899-6]
PIRⁱ	S02319, RGHUI2
RefSeqⁱ	NP_001159897.1, NM_001166425.1 [P04899-3] NP_001269546.1, NM_001282617.1 [P04899-6] NP_001269547.1, NM_001282618.1 NP_001269548.1, NM_001282619.1 [P04899-2] NP_001269549.1, NM_001282620.1 [P04899-5] NP_002061.1, NM_002070.3 [P04899-1]

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	6D9H	electron microscopy	3.60	A	1-355	[»]
SMRⁱ	P04899
ModBaseⁱ	Search...
PDBe-KBⁱ	Search...

Protein-protein interaction databases

BioGRIDⁱ	109033, 175 interactors
CORUMⁱ	P04899
DIPⁱ	DIP-602N
IntActⁱ	P04899, 78 interactors
MINTⁱ	P04899
STRINGⁱ	9606.ENSP00000312999

Chemistry databases

ChEMBLⁱ	CHEMBL4105887

ChEMBLⁱ

CHEMBL4105887

PTM databases

GlyGenⁱ	P04899, 1 site, 1 O-linked glycan (1 site)
iPTMnetⁱ	P04899
MetOSiteⁱ	P04899
PhosphoSitePlusⁱ	P04899
SwissPalmⁱ	P04899

Genetic variation databases

BioMutaⁱ	GNAI2
DMDMⁱ	121023

Proteomic databases

CPTACⁱ	CPTAC-1530 CPTAC-1531
EPDⁱ	P04899
jPOSTⁱ	P04899
MassIVEⁱ	P04899
MaxQBⁱ	P04899
PaxDbⁱ	P04899
PeptideAtlasⁱ	P04899
PRIDEⁱ	P04899
ProteomicsDBⁱ	3695 51752 [P04899-1] 51753 [P04899-2] 51754 [P04899-3] 51755 [P04899-4] 5863

Protocols and materials databases

Antibodypediaⁱ	2180, 292 antibodies
The DNASU plasmid repository More...DNASUⁱ	2771

Genome annotation databases

Ensemblⁱ	ENST00000266027; ENSP00000266027; ENSG00000114353 [P04899-6] ENST00000313601; ENSP00000312999; ENSG00000114353 [P04899-1] ENST00000422163; ENSP00000406871; ENSG00000114353 [P04899-5] ENST00000440628; ENSP00000395736; ENSG00000114353 [P04899-6] ENST00000451956; ENSP00000406369; ENSG00000114353 [P04899-3]
GeneIDⁱ	2771
KEGGⁱ	hsa:2771
UCSCⁱ	uc003cyp.3, human [P04899-1]

Organism-specific databases

CTDⁱ	2771
DisGeNETⁱ	2771
GeneCardsⁱ	GNAI2
HGNCⁱ	HGNC:4385, GNAI2
HPAⁱ	ENSG00000114353, Low tissue specificity
MalaCardsⁱ	GNAI2
MIMⁱ	139360, gene
neXtProtⁱ	NX_P04899
OpenTargetsⁱ	ENSG00000114353
PharmGKBⁱ	PA24347
VEuPathDBⁱ	HostDB:ENSG00000114353.16
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG0082, Eukaryota
GeneTreeⁱ	ENSGT00940000155125
HOGENOMⁱ	CLU_014184_6_0_1
InParanoidⁱ	P04899
OMAⁱ	PNKYEEA
PhylomeDBⁱ	P04899
TreeFamⁱ	TF300673

Enzyme and pathway databases

PathwayCommonsⁱ	P04899
Reactomeⁱ	R-HSA-112043, PLC beta mediated events R-HSA-170670, Adenylate cyclase inhibitory pathway R-HSA-202040, G-protein activation R-HSA-392170, ADP signalling through P2Y purinoceptor 12 R-HSA-400042, Adrenaline,noradrenaline inhibits insulin secretion R-HSA-418555, G alpha (s) signalling events R-HSA-418594, G alpha (i) signalling events R-HSA-418597, G alpha (z) signalling events R-HSA-422356, Regulation of insulin secretion R-HSA-6814122, Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding R-HSA-9009391, Extra-nuclear estrogen signaling R-HSA-9660821, ADORA2B mediated anti-inflammatory cytokines production
SignaLinkⁱ	P04899
SIGNORⁱ	P04899

Miscellaneous databases

BioGRID-ORCSⁱ	2771, 24 hits in 996 CRISPR screens
ChiTaRSⁱ	GNAI2, human
GeneWikiⁱ	GNAI2
GenomeRNAiⁱ	2771
Pharosⁱ	P04899, Tbio
Protein Ontology More...PROⁱ	PR:P04899
RNActⁱ	P04899, protein
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000114353, Expressed in blood and 239 other tissues
ExpressionAtlasⁱ	P04899, baseline and differential
Genevisibleⁱ	P04899, HS

Family and domain databases

CDDⁱ	cd00066, G-alpha, 1 hit
Gene3Dⁱ	1.10.400.10, 1 hit
InterProⁱ	View protein in InterPro IPR001408, Gprotein_alpha_I IPR001019, Gprotein_alpha_su IPR011025, GproteinA_insert IPR027417, P-loop_NTPase
PANTHERⁱ	PTHR10218, PTHR10218, 1 hit
Pfamⁱ	View protein in Pfam PF00503, G-alpha, 1 hit
PRINTSⁱ	PR00318, GPROTEINA PR00441, GPROTEINAI
SMARTⁱ	View protein in SMART SM00275, G_alpha, 1 hit
SUPFAMⁱ	SSF47895, SSF47895, 1 hit SSF52540, SSF52540, 1 hit
PROSITEⁱ	View protein in PROSITE PS51882, G_ALPHA, 1 hit
MobiDBⁱ	Search...

Entry informationⁱ

Entry nameⁱ	GNAI2_HUMAN
Accessionⁱ	P04899Primary (citable) accession number: P04899 Secondary accession number(s): B3KTZ0 , B4DYA0, B4E2X5, Q6B6N3, Q8IZ71
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
	Last sequence update:	January 23, 2007
	Last modified:	June 2, 2021
	This is version 231 of the entry and version 3 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Reference proteome

Documents

Human chromosome 3
Human chromosome 3: entries, gene names and cross-references to MIM
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
PDB cross-references
Index of Protein Data Bank (PDB) cross-references
SIMILARITY comments
Index of protein domains and families

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UniProtKB - P04899 (GNAI2_HUMAN)

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Guanine nucleotide-binding protein G(i) subunit alpha-2

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

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Keywords - PTMi

Proteomic databases

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P04899

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Protein-protein interaction databases

Miscellaneous databases

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3D structure databases

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Keywords - Coding sequence diversityi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ