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Protein

Guanine nucleotide-binding protein G(s) subunit alpha isoforms short

Gene

GNAS

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Guanine nucleotide-binding proteins (G proteins) function as transducers in numerous signaling pathways controlled by G protein-coupled receptors (GPCRs). Signaling involves the activation of adenylyl cyclases, resulting in increased levels of the signaling molecule cAMP (PubMed:2022671, PubMed:9395396, PubMed:11087399, PubMed:15591060, PubMed:16766715, PubMed:19243146). GNAS functions downstream of several GPCRs, including beta-adrenergic receptors. Stimulates the Ras signaling pathway via RAPGEF2 (By similarity).By similarity3 Publications3 Publications

Miscellaneous

The GNAS locus is imprinted in a complex manner, giving rise to distinct paternally, maternally and biallelically expressed proteins.
Crystal structures were determined for GNAS in complex with an adenylyl cyclase catalytic domain that was reconstructed from ADCY2 and ADCY5 N- and C-terminal domains.7 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi54Magnesium1 Publication6 Publications1
Metal bindingi204Magnesium1 Publication6 Publications1
Binding sitei366GTP; via amide nitrogen7 Publications1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi47 – 55GTP7 Publications9
Nucleotide bindingi197 – 204GTP7 Publications8
Nucleotide bindingi223 – 227GTP7 Publications5
Nucleotide bindingi292 – 295GTP7 Publications4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransducer
LigandGTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short
Alternative name(s):
Adenylate cyclase-stimulating G alpha protein
Gene namesi
Name:GNAS
Synonyms:GNAS1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00002037172 – 394Guanine nucleotide-binding protein G(s) subunit alpha isoforms shortAdd BLAST393

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-palmitoyl glycine1 Publication1
Lipidationi3S-palmitoyl cysteine1 Publication1
Cross-linki300Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei352PhosphoserineBy similarity1

Keywords - PTMi

Isopeptide bond, Lipoprotein, Palmitate, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP04896
PRIDEiP04896

PTM databases

SwissPalmiP04896

Interactioni

Subunit structurei

Heterotrimeric G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site (PubMed:9395396, PubMed:9417641, PubMed:10427002, PubMed:11087399, PubMed:15591060, PubMed:16766715, PubMed:19243146). Interacts with CRY1; the interaction may block GPCR-mediated regulation of cAMP concentrations. Interacts with ADCY6 and stimulates its adenylyl cyclase activity (By similarity). Interacts with ADCY2 and ADCY5 (PubMed:9395396, PubMed:9417641, PubMed:10427002, PubMed:11087399, PubMed:15591060, PubMed:16766715, PubMed:19243146). Stimulates the ADCY5 adenylyl cyclase activity (By similarity).By similarity7 Publications

GO - Molecular functioni

Protein-protein interaction databases

CORUMiP04896
DIPiDIP-588N
IntActiP04896, 3 interactors
STRINGi9913.ENSBTAP00000023246

Structurei

Secondary structure

1394
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP04896
SMRiP04896
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04896

Family & Domainsi

Sequence similaritiesi

Belongs to the G-alpha family. G(s) subfamily.Curated

Phylogenomic databases

eggNOGiKOG0099 Eukaryota
ENOG410XPC4 LUCA
HOGENOMiHOG000038729
HOVERGENiHBG063184
InParanoidiP04896
KOiK04632

Family and domain databases

CDDicd00066 G-alpha, 1 hit
Gene3Di1.10.400.10, 1 hit
InterProiView protein in InterPro
IPR000367 Gprotein_alpha_S
IPR001019 Gprotein_alpha_su
IPR011025 GproteinA_insert
IPR027417 P-loop_NTPase
PANTHERiPTHR10218 PTHR10218, 1 hit
PfamiView protein in Pfam
PF00503 G-alpha, 1 hit
PRINTSiPR00318 GPROTEINA
PR00443 GPROTEINAS
SMARTiView protein in SMART
SM00275 G_alpha, 1 hit
SUPFAMiSSF47895 SSF47895, 1 hit
SSF52540 SSF52540, 2 hits

Sequences (3+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.iShow all

Isoform Gnas-1 (identifier: P04896-1) [UniParc]FASTAAdd to basket
Also known as: Alpha-S2

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGCLGNSKTE DQRNEEKAQR EANKKIEKQL QKDKQVYRAT HRLLLLGAGE
60 70 80 90 100
SGKSTIVKQM RILHVNGFNG EGGEEDPQAA RSNSDGEKAT KVQDIKNNLK
110 120 130 140 150
EAIETIVAAM SNLVPPVELA NPENQFRVDY ILSVMNVPDF DFPPEFYEHA
160 170 180 190 200
KALWEDEGVR ACYERSNEYQ LIDCAQYFLD KIDVIKQDDY VPSDQDLLRC
210 220 230 240 250
RVLTSGIFET KFQVDKVNFH MFDVGGQRDE RRKWIQCFND VTAIIFVVAS
260 270 280 290 300
SSYNMVIRED NQTNRLQEAL NLFKSIWNNR WLRTISVILF LNKQDLLAEK
310 320 330 340 350
VLAGKSKIED YFPEFARYTT PEDATPEPGE DPRVTRAKYF IRDEFLRIST
360 370 380 390
ASGDGRHYCY PHFTCAVDTE NIRRVFNDCR DIIQRMHLRQ YELL
Length:394
Mass (Da):45,709
Last modified:August 13, 1987 - v1
Checksum:i4D07A734AFF2A6BC
GO
Isoform Gnas-2 (identifier: P04896-2) [UniParc]FASTAAdd to basket
Also known as: Alpha-S1

The sequence of this isoform differs from the canonical sequence as follows:
     71-84: Missing.

Show »
Length:380
Mass (Da):44,280
Checksum:i5B06C75B1A366920
GO
Isoform Nesp55 (identifier: O18979-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry O18979.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Shares no sequence similarity with other isoforms due to a novel first exon containing the entire reading frame spliced to shared exon 2 so that exons 2-13 make up the 3'-UTR.
Length:241
Mass (Da):27,494
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F1N0T0F1N0T0_BOVIN
Guanine nucleotide-binding protein ...
GNAS
347Annotation score:
G1K1V2G1K1V2_BOVIN
Guanine nucleotide-binding protein ...
GNAS
332Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti18A → G in AAA30560 (PubMed:3081893).Curated1
Sequence conflicti85D → S in AAA30560 (PubMed:3081893).Curated1
Sequence conflicti363F → S in AAA30560 (PubMed:3081893).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00183271 – 84Missing in isoform Gnas-2. CuratedAdd BLAST14

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03404 mRNA Translation: CAA27137.1
M13006 mRNA Translation: AAA30560.1
BC102905 mRNA Translation: AAI02906.1
BC122757 mRNA Translation: AAI22758.1
M14014 mRNA Translation: AAA30557.1
PIRiA23818 RGBOGA
I45904
RefSeqiNP_001258700.1, NM_001271771.1
NP_851364.1, NM_181021.3 [P04896-1]
UniGeneiBt.64619
Bt.91987

Genome annotation databases

GeneIDi281793
KEGGibta:281793

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiGNAS2_BOVIN
AccessioniPrimary (citable) accession number: P04896
Secondary accession number(s): Q0II85, Q3SZE7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: July 18, 2018
This is version 179 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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