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Protein

C' protein

Gene

P/V/C

Organism
Sendai virus (strain Z) (SeV) (Sendai virus (strain HVJ))
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The different isoforms prevent the establishment of cellular antiviral state by blocking the interferon-alpha/beta (IFN-alpha/beta) and IFN-gamma signaling pathways. They inhibit IFN-alpha/beta induced tyrosine phosphorylation of STAT1 and STAT2. Blocking the IFN-alpha/beta pathway requires binding to STAT1 in the cytoplasm. They inhibit IFN-gamma induced serine phosphorylation of STAT1. Block the IFN-gamma pathway by binding to and stabilizing the parallel form of the STAT1 dimer, further inducing high-molecular-weight complex (HMWC) formation and inhibition of transcription by IFN-gamma. May also have a role in preventing the cell to enter apoptosis. Modulate regulation of viral transcription and replication. Overexpression inhibits the viral RNA polymerase. The absence of all C', C, Y1 and Y2 proteins leads to viral delayed growth. Plays an important role in virion particles release. Modulates virion shape.8 Publications

Miscellaneous

The P/V/C gene has two overlapping open reading frames. One encodes the P/V/W proteins and the other the C/Y proteins.Curated

Caution

The C' protein uses an unusual ACG start codon.Curated

GO - Biological processi

Keywordsi

Biological processHost-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host STAT1 by virus, Inhibition of host STAT2 by virus, Interferon antiviral system evasion, Viral immunoevasion

Names & Taxonomyi

Protein namesi
Recommended name:
C' protein
Gene namesi
Name:P/V/C
OrganismiSendai virus (strain Z) (SeV) (Sendai virus (strain HVJ))
Taxonomic identifieri11198 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesMononegaviralesParamyxoviridaeRespirovirus
Virus hostiCavia cutleri (Guinea pig) [TaxID: 10144]
Cricetidae sp. (Hamster) [TaxID: 36483]
Mus musculus (Mouse) [TaxID: 10090]
Rattus norvegicus (Rat) [TaxID: 10116]
Proteomesi
  • UP000006560 Componenti: Genome

Subcellular locationi

Isoform C' protein :
Isoform C protein :

GO - Cellular componenti

Keywords - Cellular componenti

Host cytoplasm, Virion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi28 – 30EDE → AAA: 11% loss of binding to L protein in protein C'. 1 Publication3
Mutagenesisi88 – 91KIID → AIIA: Complete loss of STAT1-binding and RNA synthesis inhibition by C protein. 80% loss of binding to L protein in protein C'. 2 Publications4
Mutagenesisi125 – 126EE → AA: Complete loss of STAT1-binding in protein C. No effect on binding to L protein in protein C'. 2 Publications2
Mutagenesisi140 – 143ETPE → ATPA: 60% loss of binding to L protein in protein C'. 1 Publication4
Mutagenesisi150 – 153MRED → AREA: Complete loss of STAT1-binding and RNA synthesis inhibition by C protein. 2 Publications4
Mutagenesisi162 – 165KTER → ATAA: Complete loss of STAT1-binding, anti-IFN activity and RNA synthesis inhibition by protein C. 67% loss of binding to L protein in C' protein. 2 Publications4
Mutagenesisi168R → A: 63% loss of binding to L protein in protein C'. 1 Publication1
Mutagenesisi172 – 174RGE → AGA: 10% loss of binding to L protein. 1 Publication3
Mutagenesisi181F → S: Complete loss of STAT1-binding by C protein. 49% loss of binding to L protein. 2 Publications1
Mutagenesisi184 – 187RYEE → AYAA: Complete loss of STAT1-binding and RNA synthesis inhibition by C protein. 62% loss of binding to L protein. 2 Publications4
Mutagenesisi203 – 204EE → AA: 17% loss of binding to L protein. 1 Publication2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved; by hostBy similarity
ChainiPRO_00000394112 – 215C' proteinAdd BLAST214

Post-translational modificationi

Y1 and Y2 proteins are produced not only by alternative initiation, but also by proteolytic cleavage of C'. Only alternative initiation is detected in vitro, whereas in vivo cleavage seems to be predominant.By similarity

Interactioni

Subunit structurei

The different isoforms interact (via C-terminus) with unphosphorylated and phosphorylated human STAT1 (via N-terminus), favoring the formation of parallel STAT1 homodimers (PubMed:11442634, PubMed:26339056). The different isoforms do not interact with host STAT2 (PubMed:11442634, PubMed:26339056). C protein interacts with L protein; this interaction has an inhibitory effect on viral transcription and replication (PubMed:9281506, PubMed:11543662).4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ChukQ606802EBI-8848010,EBI-646245From Mus musculus.

Protein-protein interaction databases

IntActiP04862, 2 interactors
MINTiP04862

Structurei

Secondary structure

1215
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SMRiP04862
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni12 – 34DisorderedBy similarityAdd BLAST23
Regioni15 – 22Involved in self-degradation and in host STAT1 degradationBy similarity8

Domaini

The disordered region at the N-terminus is involved in C protein self-degradation in trans. This self-degradation of C protein may play a role in the regulation of viral RNA synthesis. The disordered region at the N-terminus is also involved in the host STAT1 degradation in order to counteract the host innate antiviral response.By similarity

Sequence similaritiesi

Belongs to the respirovirus protein C family.Curated

Phylogenomic databases

OrthoDBiVOG090000QC

Family and domain databases

InterProiView protein in InterPro
IPR002608 Paramyxo_C
PfamiView protein in Pfam
PF01692 Paramyxo_C, 1 hit

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative initiation. AlignAdd to basket
Isoform C' protein (identifier: P04862-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MASATLTAWI KMPSFLKKIL KLRGRRQEDE SRSRMLSDSS MLSCRVNQLT
60 70 80 90 100
SEGTEAGSTT PSTLPKDQAL LIEPKVRAKE KSQHRRPKII DQVRRVESLG
110 120 130 140 150
EQASQRQKHM LETLINKIYT GPLGEELVQT LYLRIWAMEE TPESLKILQM
160 170 180 190 200
REDIRDQVLK MKTERWLRTL IRGEKTKLKD FQKRYEEVHP YLMKEKVEQV
210
IMEEAWSLAA HIVQE
Note: The initiator methionine is coded by an unusual start codon ACG.
Length:215
Mass (Da):25,189
Last modified:January 23, 2007 - v3
Checksum:i506F01080EB9BEDC
GO
Isoform C protein (identifier: P04862-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-11: Missing.

Note: Most abundant isoform in infected cells.
Show »
Length:204
Mass (Da):24,015
Checksum:i3F4AAEC228222857
GO
Isoform Y1 protein (identifier: P04862-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-34: Missing.

Note: No experimental confirmation available.
Show »
Length:181
Mass (Da):21,202
Checksum:i0817DE79BF6130E7
GO
Isoform Y2 protein (identifier: P04862-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-40: Missing.

Note: No experimental confirmation available.
Show »
Length:175
Mass (Da):20,581
Checksum:i2767937BC46BAA50
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0189461 – 40Missing in isoform Y2 protein. CuratedAdd BLAST40
Alternative sequenceiVSP_0189451 – 34Missing in isoform Y1 protein. CuratedAdd BLAST34
Alternative sequenceiVSP_0189441 – 11Missing in isoform C protein. CuratedAdd BLAST11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00087 Genomic RNA Translation: CAA24947.1
PIRiA04041 MNNZSV

Keywords - Coding sequence diversityi

Alternative initiation

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00087 Genomic RNA Translation: CAA24947.1
PIRiA04041 MNNZSV

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3WWTX-ray2.00B109-215[»]
SMRiP04862
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP04862, 2 interactors
MINTiP04862

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

OrthoDBiVOG090000QC

Family and domain databases

InterProiView protein in InterPro
IPR002608 Paramyxo_C
PfamiView protein in Pfam
PF01692 Paramyxo_C, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiC_SENDZ
AccessioniPrimary (citable) accession number: P04862
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: January 23, 2007
Last modified: February 28, 2018
This is version 70 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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