P/V/C

Functionⁱ

The different isoforms prevent the establishment of cellular antiviral state by blocking the interferon-alpha/beta (IFN-alpha/beta) and IFN-gamma signaling pathways. They inhibit IFN-alpha/beta induced tyrosine phosphorylation of STAT1 and STAT2. Blocking the IFN-alpha/beta pathway requires binding to STAT1 in the cytoplasm. They inhibit IFN-gamma induced serine phosphorylation of STAT1. Block the IFN-gamma pathway by binding to and stabilizing the parallel form of the STAT1 dimer, further inducing high-molecular-weight complex (HMWC) formation and inhibition of transcription by IFN-gamma. May also have a role in preventing the cell to enter apoptosis. Modulate regulation of viral transcription and replication. Overexpression inhibits the viral RNA polymerase. The absence of all C', C, Y1 and Y2 proteins leads to viral delayed growth. Plays an important role in virion particles release. Modulates virion shape.8 Publications

Miscellaneous

The P/V/C gene has two overlapping open reading frames. One encodes the P/V/W proteins and the other the C/Y proteins.Curated

Caution

The C' protein uses an unusual ACG start codon.Curated

GO - Biological processⁱ

suppression by virus of host STAT1 activity
Source: UniProtKB
Inferred from direct assayⁱ
- 11821064
suppression by virus of host STAT2 activity Source: UniProtKB-KW
suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host STAT1 by virus, Inhibition of host STAT2 by virus, Interferon antiviral system evasion, Viral immunoevasion

Biological process

Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host STAT1 by virus, Inhibition of host STAT2 by virus, Interferon antiviral system evasion, Viral immunoevasion

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: C' protein
Gene namesⁱ	Name:P/V/C
Organismⁱ	Sendai virus (strain Z) (SeV) (Sendai virus (strain HVJ))
Taxonomic identifierⁱ	11198 [NCBI]
Taxonomic lineageⁱ	Viruses › ssRNA viruses › ssRNA negative-strand viruses › Mononegavirales › Paramyxoviridae › Respirovirus › Murine respirovirus
Virus hostⁱ	Cavia cutleri (Guinea pig) [TaxID: 10144] Cricetidae sp. (Hamster) [TaxID: 36483] Mus musculus (Mouse) [TaxID: 10090] Rattus norvegicus (Rat) [TaxID: 10116]
Proteomesⁱ	UP000006560 Componentⁱ: Genome

Subcellular locationⁱ

Isoform C' protein :

Host cytoplasm
2 Publications
Manual assertion based on experiment inⁱ
- Ref.2
  "Localization and characterization of Sendai virus nonstructural C and C' proteins by antibodies against synthetic peptides."
  Portner A., Gupta K.C., Seyer J.M., Beachey E.H., Kingsbury D.W.
  Virus Res. 6:109-121(1986) [PubMed] [Europe PMC] [Abstract]
  Cited for: SUBCELLULAR LOCATION.
- Ref.3
  "Association of the Sendai virus C protein with nucleocapsids."
  Yamada H., Hayata S., Omata-Yamada T., Taira H., Mizumoto K., Iwasaki K.
  Arch. Virol. 113:245-253(1990) [PubMed] [Europe PMC] [Abstract]
  Cited for: SUBCELLULAR LOCATION.

Note:

Protein C' seems to localize around the Golgi.1 Publication

Isoform C protein :

Note:

The C protein is found in virion at a ratio of approximately 40 molecules per virion, presumably associated with the nucleocapsid.1 Publication

GO - Cellular componentⁱ

host cell cytoplasm Source: UniProtKB-SubCell
virion Source: UniProtKB-SubCell

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Host cytoplasm, Virion

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	28 – 30	EDE → AAA: 11% loss of binding to L protein in protein C'. 1 Publication	3
Mutagenesisⁱ	88 – 91	KIID → AIIA: Complete loss of STAT1-binding and RNA synthesis inhibition by C protein. 80% loss of binding to L protein in protein C'. 2 Publications	4
Mutagenesisⁱ	125 – 126	EE → AA: Complete loss of STAT1-binding in protein C. No effect on binding to L protein in protein C'. 2 Publications	2
Mutagenesisⁱ	140 – 143	ETPE → ATPA: 60% loss of binding to L protein in protein C'. 1 Publication	4
Mutagenesisⁱ	150 – 153	MRED → AREA: Complete loss of STAT1-binding and RNA synthesis inhibition by C protein. 2 Publications	4
Mutagenesisⁱ	162 – 165	KTER → ATAA: Complete loss of STAT1-binding, anti-IFN activity and RNA synthesis inhibition by protein C. 67% loss of binding to L protein in C' protein. 2 Publications	4
Mutagenesisⁱ	168	R → A: 63% loss of binding to L protein in protein C'. 1 Publication	1
Mutagenesisⁱ	172 – 174	RGE → AGA: 10% loss of binding to L protein. 1 Publication	3
Mutagenesisⁱ	181	F → S: Complete loss of STAT1-binding by C protein. 49% loss of binding to L protein. 2 Publications	1
Mutagenesisⁱ	184 – 187	RYEE → AYAA: Complete loss of STAT1-binding and RNA synthesis inhibition by C protein. 62% loss of binding to L protein. 2 Publications	4
Mutagenesisⁱ	203 – 204	EE → AA: 17% loss of binding to L protein. 1 Publication	2

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Initiator methionineⁱ		Removed; by hostBy similarity
ChainⁱPRO_0000039411	2 – 215	C' proteinAdd BLAST		214

Post-translational modificationⁱ

Y1 and Y2 proteins are produced not only by alternative initiation, but also by proteolytic cleavage of C'. Only alternative initiation is detected in vitro, whereas in vivo cleavage seems to be predominant.By similarity

Interactionⁱ

Subunit structureⁱ

The different isoforms interact (via C-terminus) with unphosphorylated and phosphorylated human STAT1 (via N-terminus), favoring the formation of parallel STAT1 homodimers (PubMed:11442634, PubMed:26339056). The different isoforms do not interact with host STAT2 (PubMed:11442634, PubMed:26339056). C protein interacts with L protein; this interaction has an inhibitory effect on viral transcription and replication (PubMed:9281506, PubMed:11543662).4 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
Chuk	Q60680	2	EBI-8848010,EBI-646245	From Mus musculus.

With

Entry

#Exp.

IntAct

Notes

Chuk

Q60680

2

EBI-8848010,EBI-646245

From Mus musculus.

Protein-protein interaction databases

IntActⁱ	P04862, 2 interactors
Molecular INTeraction database More...MINTⁱ	P04862

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	109 – 120	Combined sources	12
Helixⁱ	122 – 137	Combined sources	16
Helixⁱ	142 – 149	Combined sources	8
Helixⁱ	152 – 170	Combined sources	19
Helixⁱ	180 – 194	Combined sources	15
Helixⁱ	196 – 210	Combined sources	15

Show more details

3D structure databases

SMRⁱ	P04862
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	12 – 34	DisorderedBy similarityAdd BLAST		23
Regionⁱ	15 – 22	Involved in self-degradation and in host STAT1 degradationBy similarity		8

Domainⁱ

The disordered region at the N-terminus is involved in C protein self-degradation in trans. This self-degradation of C protein may play a role in the regulation of viral RNA synthesis. The disordered region at the N-terminus is also involved in the host STAT1 degradation in order to counteract the host innate antiviral response.By similarity

Sequence similaritiesⁱ

Belongs to the respirovirus protein C family.Curated

Phylogenomic databases

Database of Orthologous Groups More...OrthoDBⁱ	VOG090000QC

OrthoDBⁱ

VOG090000QC

Family and domain databases

InterProⁱ	View protein in InterPro IPR002608 Paramyxo_C
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01692 Paramyxo_C, 1 hit

Sequences (4)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsⁱ produced by alternative initiation. Align Add to basket

Isoform C' protein (identifier: P04862-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASATLTAWI KMPSFLKKIL KLRGRRQEDE SRSRMLSDSS MLSCRVNQLT 
        60         70         80         90        100
SEGTEAGSTT PSTLPKDQAL LIEPKVRAKE KSQHRRPKII DQVRRVESLG 
       110        120        130        140        150
EQASQRQKHM LETLINKIYT GPLGEELVQT LYLRIWAMEE TPESLKILQM 
       160        170        180        190        200
REDIRDQVLK MKTERWLRTL IRGEKTKLKD FQKRYEEVHP YLMKEKVEQV 
       210 
IMEEAWSLAA HIVQE

Note: The initiator methionine is coded by an unusual start codon ACG.

Length:215

Mass (Da):25,189

Last modified:January 23, 2007 - v3

Checksum:ⁱ506F01080EB9BEDC

GO

Isoform C protein (identifier: P04862-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
1-11: Missing.

« Hide

        10         20         30         40         50
MPSFLKKILK LRGRRQEDES RSRMLSDSSM LSCRVNQLTS EGTEAGSTTP 
        60         70         80         90        100
STLPKDQALL IEPKVRAKEK SQHRRPKIID QVRRVESLGE QASQRQKHML 
       110        120        130        140        150
ETLINKIYTG PLGEELVQTL YLRIWAMEET PESLKILQMR EDIRDQVLKM 
       160        170        180        190        200
KTERWLRTLI RGEKTKLKDF QKRYEEVHPY LMKEKVEQVI MEEAWSLAAH 

IVQE

Note: Most abundant isoform in infected cells.

Show »

Length:204

Mass (Da):24,015

Checksum:ⁱ3F4AAEC228222857

GO

Isoform Y1 protein (identifier: P04862-3) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
1-34: Missing.

« Hide

        10         20         30         40         50
MLSDSSMLSC RVNQLTSEGT EAGSTTPSTL PKDQALLIEP KVRAKEKSQH 
        60         70         80         90        100
RRPKIIDQVR RVESLGEQAS QRQKHMLETL INKIYTGPLG EELVQTLYLR 
       110        120        130        140        150
IWAMEETPES LKILQMREDI RDQVLKMKTE RWLRTLIRGE KTKLKDFQKR 
       160        170        180 
YEEVHPYLMK EKVEQVIMEE AWSLAAHIVQ E

Note: No experimental confirmation available.

Show »

Length:181

Mass (Da):21,202

Checksum:ⁱ0817DE79BF6130E7

GO

Isoform Y2 protein (identifier: P04862-4) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
1-40: Missing.

« Hide

        10         20         30         40         50
MLSCRVNQLT SEGTEAGSTT PSTLPKDQAL LIEPKVRAKE KSQHRRPKII 
        60         70         80         90        100
DQVRRVESLG EQASQRQKHM LETLINKIYT GPLGEELVQT LYLRIWAMEE 
       110        120        130        140        150
TPESLKILQM REDIRDQVLK MKTERWLRTL IRGEKTKLKD FQKRYEEVHP 
       160        170 
YLMKEKVEQV IMEEAWSLAA HIVQE

Note: No experimental confirmation available.

Show »

Length:175

Mass (Da):20,581

Checksum:ⁱ2767937BC46BAA50

GO

Alternative sequence

Feature key	Position(s)	DescriptionActions	Length
Alternative sequenceⁱVSP_018946	1 – 40	Missing in isoform Y2 protein. CuratedAdd BLAST	40
Alternative sequenceⁱVSP_018945	1 – 34	Missing in isoform Y1 protein. CuratedAdd BLAST	34
Alternative sequenceⁱVSP_018944	1 – 11	Missing in isoform C protein. CuratedAdd BLAST	11

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X00087 Genomic RNA Translation: CAA24947.1
PIRⁱ	A04041 MNNZSV

Keywords - Coding sequence diversityⁱ

Alternative initiation

Protein	Similar proteins		Species	Score	Length	Source
P04862	Isoform C protein of C' protein		SENDZ		204	UniRef100_P04862
Isoform Y1 protein of C' protein		SENDZ		181
P04862-4	Protein C'		SEND6		215	UniRef100_P14253
Isoform C of Protein C'		SEND6		204
Isoform Y2 of Protein C'		SEND6		175

Protein	Similar proteins		Species	Score	Length	Source
P04862	Protein C'		SEND6		215	UniRef90_P04862
Protein C'		SENDA		215
Protein C'		SENDF		215
Protein C'		SENDH		215
Protein C'		SENDN		215
+28

Protein	Similar proteins		Species	Score	Length	Source
P04862	Protein C'		SENDF		215	UniRef50_P04862
Protein C'		SENDA		215
Protein C'		SEND6		215
Protein C'		SENDO		215
Protein C'		SENDH		215
+104

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X00087 Genomic RNA Translation: CAA24947.1
PIRⁱ	A04041 MNNZSV

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	3WWT	X-ray	2.00	B	109-215	[»]
SMRⁱ	P04862
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

IntActⁱ	P04862, 2 interactors
MINTⁱ	P04862

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Phylogenomic databases

OrthoDBⁱ	VOG090000QC

OrthoDBⁱ

VOG090000QC

Family and domain databases

InterProⁱ	View protein in InterPro IPR002608 Paramyxo_C
Pfamⁱ	View protein in Pfam PF01692 Paramyxo_C, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	C_SENDZ
Accessionⁱ	P04862Primary (citable) accession number: P04862
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
	Last sequence update:	January 23, 2007
	Last modified:	February 28, 2018
	This is version 70 of the entry and version 3 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Viral Protein Annotation Program

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P04862 (C_SENDZ)

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C' protein

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

Caution

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Alternative sequence

Sequence databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Phylogenomic databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ