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Protein

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2

Gene

RPN2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.By similarityCurated

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • aging Source: Ensembl
  • cellular protein modification process Source: ProtInc
  • protein N-linked glycosylation Source: GO_Central
  • protein N-linked glycosylation via asparagine Source: HGNC
  • response to drug Source: Ensembl

Enzyme and pathway databases

ReactomeiR-HSA-1799339 SRP-dependent cotranslational protein targeting to membrane
R-HSA-446203 Asparagine N-linked glycosylation
UniPathwayi
UPA00378

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2
Alternative name(s):
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa subunit
RIBIIR
Ribophorin II
Short name:
RPN-II
Ribophorin-2
Gene namesi
Name:RPN2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

EuPathDBiHostDB:ENSG00000118705.16
HGNCiHGNC:10382 RPN2
MIMi180490 gene
neXtProtiNX_P04844

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini23 – 540LumenalSequence analysisAdd BLAST518
Transmembranei541 – 561HelicalSequence analysisAdd BLAST21
Topological domaini562 – 571CytoplasmicSequence analysis10
Transmembranei572 – 592HelicalSequence analysisAdd BLAST21
Topological domaini593 – 596LumenalSequence analysis4
Transmembranei597 – 617HelicalSequence analysisAdd BLAST21
Topological domaini618 – 631CytoplasmicSequence analysisAdd BLAST14

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi6185
OpenTargetsiENSG00000118705
PharmGKBiPA34778

Polymorphism and mutation databases

BioMutaiRPN2
DMDMi9297108

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 221 PublicationAdd BLAST22
ChainiPRO_000002224423 – 631Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2Add BLAST609

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi106N-linked (GlcNAc...) asparagine1 Publication1
Cross-linki154Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Keywords - PTMi

Glycoprotein, Isopeptide bond, Ubl conjugation

Proteomic databases

EPDiP04844
MaxQBiP04844
PaxDbiP04844
PeptideAtlasiP04844
PRIDEiP04844
ProteomicsDBi51750
51751 [P04844-2]
TopDownProteomicsiP04844-1 [P04844-1]

PTM databases

GlyConnecti1187
iPTMnetiP04844
PhosphoSitePlusiP04844
SwissPalmiP04844

Expressioni

Tissue specificityi

Expressed in all tissues tested.

Gene expression databases

BgeeiENSG00000118705 Expressed in 244 organ(s), highest expression level in caput epididymis
CleanExiHS_RPN2
ExpressionAtlasiP04844 baseline and differential
GenevisibleiP04844 HS

Organism-specific databases

HPAiCAB019277
HPA008297
HPA025922

Interactioni

Subunit structurei

Component of the oligosaccharyltransferase (OST) complex. OST exists in two different complex forms which contain common core subunits RPN1, RPN2, OST48, OST4, DAD1 and TMEM258, either STT3A or STT3B as catalytic subunits, and form-specific accessory subunits. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes.By similarityCurated2 Publications

Protein-protein interaction databases

BioGridi112100, 105 interactors
CORUMiP04844
IntActiP04844, 54 interactors
MINTiP04844
STRINGi9606.ENSP00000237530

Structurei

3D structure databases

ProteinModelPortaliP04844
SMRiP04844
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SWP1 family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2447 Eukaryota
ENOG410XRF2 LUCA
GeneTreeiENSGT00390000002635
HOGENOMiHOG000231261
HOVERGENiHBG002365
InParanoidiP04844
KOiK12667
OMAiPKPEIQH
OrthoDBiEOG091G0H3U
PhylomeDBiP04844
TreeFamiTF106146

Family and domain databases

InterProiView protein in InterPro
IPR008814 Swp1
PANTHERiPTHR12640 PTHR12640, 1 hit
PfamiView protein in Pfam
PF05817 Ribophorin_II, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P04844-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAPPGSSTVF LLALTIIAST WALTPTHYLT KHDVERLKAS LDRPFTNLES
60 70 80 90 100
AFYSIVGLSS LGAQVPDAKK ACTYIRSNLD PSNVDSLFYA AQASQALSGC
110 120 130 140 150
EISISNETKD LLLAAVSEDS SVTQIYHAVA ALSGFGLPLA SQEALSALTA
160 170 180 190 200
RLSKEETVLA TVQALQTASH LSQQADLRSI VEEIEDLVAR LDELGGVYLQ
210 220 230 240 250
FEEGLETTAL FVAATYKLMD HVGTEPSIKE DQVIQLMNAI FSKKNFESLS
260 270 280 290 300
EAFSVASAAA VLSHNRYHVP VVVVPEGSAS DTHEQAILRL QVTNVLSQPL
310 320 330 340 350
TQATVKLEHA KSVASRATVL QKTSFTPVGD VFELNFMNVK FSSGYYDFLV
360 370 380 390 400
EVEGDNRYIA NTVELRVKIS TEVGITNVDL STVDKDQSIA PKTTRVTYPA
410 420 430 440 450
KAKGTFIADS HQNFALFFQL VDVNTGAELT PHQTFVRLHN QKTGQEVVFV
460 470 480 490 500
AEPDNKNVYK FELDTSERKI EFDSASGTYT LYLIIGDATL KNPILWNVAD
510 520 530 540 550
VVIKFPEEEA PSTVLSQNLF TPKQEIQHLF REPEKRPPTV VSNTFTALIL
560 570 580 590 600
SPLLLLFALW IRIGANVSNF TFAPSTIIFH LGHAAMLGLM YVYWTQLNMF
610 620 630
QTLKYLAILG SVTFLAGNRM LAQQAVKRTA H
Length:631
Mass (Da):69,284
Last modified:December 1, 2000 - v3
Checksum:iE24D7B3565141676
GO
Isoform 2 (identifier: P04844-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     70-101: Missing.
     627-627: K → KRIAAEQSSRLAKYRTL

Note: No experimental confirmation available.
Show »
Length:615
Mass (Da):67,723
Checksum:iFC967C1FA17A011E
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q5JYR7Q5JYR7_HUMAN
Dolichyl-diphosphooligosaccharide--...
RPN2
344Annotation score:
H0Y5M1H0Y5M1_HUMAN
Dolichyl-diphosphooligosaccharide--...
RPN2
147Annotation score:
Q5JYR3Q5JYR3_HUMAN
Dolichyl-diphosphooligosaccharide--...
RPN2
138Annotation score:
F2Z3K5F2Z3K5_HUMAN
Dolichyl-diphosphooligosaccharide--...
RPN2
162Annotation score:
Q5JYR4Q5JYR4_HUMAN
Dolichyl-diphosphooligosaccharide--...
RPN2
166Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti197V → L in CAA68393 (PubMed:3034581).Curated1
Sequence conflicti201F → C in CAA68393 (PubMed:3034581).Curated1
Sequence conflicti260A → S in CAA68393 (PubMed:3034581).Curated1
Sequence conflicti286A → S in CAG33180 (Ref. 4) Curated1
Sequence conflicti423V → M in CAA68393 (PubMed:3034581).Curated1
Sequence conflicti427A → V in CAG33180 (Ref. 4) Curated1
Sequence conflicti571T → I in AAH13028 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_054040597L → F. Corresponds to variant dbSNP:rs34951322Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04305170 – 101Missing in isoform 2. 1 PublicationAdd BLAST32
Alternative sequenceiVSP_043052627K → KRIAAEQSSRLAKYRTL in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00282 mRNA Translation: CAA68393.1
AJ237734
, AJ237735, AJ237733, AJ237736, AJ237737, AJ237738, AJ237739, AJ237740, AJ237741, AJ237742, AJ237743, AJ237744, AJ237745, AJ237746, AJ237747, AJ237748, AJ237749 Genomic DNA Translation: CAB54801.1
AK096243 mRNA Translation: BAG53237.1
CR456899 mRNA Translation: CAG33180.1
AL031659 Genomic DNA No translation available.
CH471077 Genomic DNA Translation: EAW76073.1
BC002380 mRNA Translation: AAH02380.2
BC003560 mRNA Translation: AAH03560.1
BC013028 mRNA Translation: AAH13028.2
BC020222 mRNA Translation: AAH20222.1
CCDSiCCDS13291.1 [P04844-1]
CCDS46599.1 [P04844-2]
PIRiB26168
RefSeqiNP_001129243.1, NM_001135771.2 [P04844-2]
NP_001311228.1, NM_001324299.1
NP_001311230.1, NM_001324301.1
NP_001311231.1, NM_001324302.1
NP_001311232.1, NM_001324303.1
NP_001311233.1, NM_001324304.1
NP_001311234.1, NM_001324305.1
NP_001311235.1, NM_001324306.1
NP_002942.2, NM_002951.4 [P04844-1]
UniGeneiHs.370895

Genome annotation databases

EnsembliENST00000237530; ENSP00000237530; ENSG00000118705 [P04844-1]
ENST00000373622; ENSP00000362724; ENSG00000118705 [P04844-2]
GeneIDi6185
KEGGihsa:6185
UCSCiuc002xgp.4 human [P04844-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00282 mRNA Translation: CAA68393.1
AJ237734
, AJ237735, AJ237733, AJ237736, AJ237737, AJ237738, AJ237739, AJ237740, AJ237741, AJ237742, AJ237743, AJ237744, AJ237745, AJ237746, AJ237747, AJ237748, AJ237749 Genomic DNA Translation: CAB54801.1
AK096243 mRNA Translation: BAG53237.1
CR456899 mRNA Translation: CAG33180.1
AL031659 Genomic DNA No translation available.
CH471077 Genomic DNA Translation: EAW76073.1
BC002380 mRNA Translation: AAH02380.2
BC003560 mRNA Translation: AAH03560.1
BC013028 mRNA Translation: AAH13028.2
BC020222 mRNA Translation: AAH20222.1
CCDSiCCDS13291.1 [P04844-1]
CCDS46599.1 [P04844-2]
PIRiB26168
RefSeqiNP_001129243.1, NM_001135771.2 [P04844-2]
NP_001311228.1, NM_001324299.1
NP_001311230.1, NM_001324301.1
NP_001311231.1, NM_001324302.1
NP_001311232.1, NM_001324303.1
NP_001311233.1, NM_001324304.1
NP_001311234.1, NM_001324305.1
NP_001311235.1, NM_001324306.1
NP_002942.2, NM_002951.4 [P04844-1]
UniGeneiHs.370895

3D structure databases

ProteinModelPortaliP04844
SMRiP04844
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112100, 105 interactors
CORUMiP04844
IntActiP04844, 54 interactors
MINTiP04844
STRINGi9606.ENSP00000237530

PTM databases

GlyConnecti1187
iPTMnetiP04844
PhosphoSitePlusiP04844
SwissPalmiP04844

Polymorphism and mutation databases

BioMutaiRPN2
DMDMi9297108

Proteomic databases

EPDiP04844
MaxQBiP04844
PaxDbiP04844
PeptideAtlasiP04844
PRIDEiP04844
ProteomicsDBi51750
51751 [P04844-2]
TopDownProteomicsiP04844-1 [P04844-1]

Protocols and materials databases

DNASUi6185
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000237530; ENSP00000237530; ENSG00000118705 [P04844-1]
ENST00000373622; ENSP00000362724; ENSG00000118705 [P04844-2]
GeneIDi6185
KEGGihsa:6185
UCSCiuc002xgp.4 human [P04844-1]

Organism-specific databases

CTDi6185
DisGeNETi6185
EuPathDBiHostDB:ENSG00000118705.16
GeneCardsiRPN2
HGNCiHGNC:10382 RPN2
HPAiCAB019277
HPA008297
HPA025922
MIMi180490 gene
neXtProtiNX_P04844
OpenTargetsiENSG00000118705
PharmGKBiPA34778
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2447 Eukaryota
ENOG410XRF2 LUCA
GeneTreeiENSGT00390000002635
HOGENOMiHOG000231261
HOVERGENiHBG002365
InParanoidiP04844
KOiK12667
OMAiPKPEIQH
OrthoDBiEOG091G0H3U
PhylomeDBiP04844
TreeFamiTF106146

Enzyme and pathway databases

UniPathwayi
UPA00378

ReactomeiR-HSA-1799339 SRP-dependent cotranslational protein targeting to membrane
R-HSA-446203 Asparagine N-linked glycosylation

Miscellaneous databases

ChiTaRSiRPN2 human
GeneWikiiRPN2
GenomeRNAii6185
PROiPR:P04844
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000118705 Expressed in 244 organ(s), highest expression level in caput epididymis
CleanExiHS_RPN2
ExpressionAtlasiP04844 baseline and differential
GenevisibleiP04844 HS

Family and domain databases

InterProiView protein in InterPro
IPR008814 Swp1
PANTHERiPTHR12640 PTHR12640, 1 hit
PfamiView protein in Pfam
PF05817 Ribophorin_II, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRPN2_HUMAN
AccessioniPrimary (citable) accession number: P04844
Secondary accession number(s): Q5JYR6
, Q6IBA5, Q96E21, Q9BUQ3, Q9UBE1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: December 1, 2000
Last modified: November 7, 2018
This is version 186 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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