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Protein

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1

Gene

RPN1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.By similarityCurated

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • cellular protein modification process Source: ProtInc
  • protein N-linked glycosylation via asparagine Source: UniProtKB

Enzyme and pathway databases

ReactomeiR-HSA-1799339 SRP-dependent cotranslational protein targeting to membrane
R-HSA-446203 Asparagine N-linked glycosylation
SIGNORiP04843
UniPathwayi
UPA00378

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
Alternative name(s):
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit
Ribophorin I
Short name:
RPN-I
Ribophorin-1
Gene namesi
Name:RPN1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

EuPathDBiHostDB:ENSG00000163902.11
HGNCiHGNC:10381 RPN1
MIMi180470 gene
neXtProtiNX_P04843

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini24 – 438LumenalSequence analysisAdd BLAST415
Transmembranei439 – 457HelicalSequence analysisAdd BLAST19
Topological domaini458 – 607CytoplasmicSequence analysisAdd BLAST150

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi6184
MalaCardsiRPN1
OpenTargetsiENSG00000163902
Orphaneti402020 'Acute myeloid leukemia with inv3(p21;q26.2) or t(3;3)(p21;q26.2)'
PharmGKBiPA34777

Polymorphism and mutation databases

BioMutaiRPN1
DMDMi132559

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Add BLAST23
ChainiPRO_000002224124 – 607Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1Add BLAST584

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei187N6-acetyllysineCombined sources1
Glycosylationi299N-linked (GlcNAc...) asparagine1 Publication1
Modified residuei538N6-acetyllysine; alternateBy similarity1
Cross-linki538Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources

Keywords - PTMi

Acetylation, Glycoprotein, Isopeptide bond, Ubl conjugation

Proteomic databases

EPDiP04843
MaxQBiP04843
PaxDbiP04843
PeptideAtlasiP04843
PRIDEiP04843
ProteomicsDBi51749
TopDownProteomicsiP04843

PTM databases

GlyConnecti1186
iPTMnetiP04843
PhosphoSitePlusiP04843
SwissPalmiP04843

Expressioni

Tissue specificityi

Expressed in all tissues tested.1 Publication

Gene expression databases

BgeeiENSG00000163902 Expressed in 232 organ(s), highest expression level in corpus epididymis
CleanExiHS_RPN1
ExpressionAtlasiP04843 baseline and differential
GenevisibleiP04843 HS

Organism-specific databases

HPAiCAB009748
HPA026828
HPA051520

Interactioni

Subunit structurei

Component of the oligosaccharyltransferase (OST) complex. OST exists in two different complex forms which contain common core subunits RPN1, RPN2, OST48, OST4, DAD1 and TMEM258, either STT3A or STT3B as catalytic subunits, and form-specific accessory subunits. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes (By similarity).By similarityCurated2 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi112099, 160 interactors
CORUMiP04843
DIPiDIP-38152N
IntActiP04843, 55 interactors
MINTiP04843
STRINGi9606.ENSP00000296255

Structurei

3D structure databases

ProteinModelPortaliP04843
SMRiP04843
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the OST1 family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2291 Eukaryota
ENOG410XQVZ LUCA
GeneTreeiENSGT00390000009630
HOGENOMiHOG000247000
HOVERGENiHBG012864
InParanoidiP04843
KOiK12666
OMAiRYPIFGG
OrthoDBiEOG091G05K5
PhylomeDBiP04843
TreeFamiTF312988

Family and domain databases

InterProiView protein in InterPro
IPR007676 Ribophorin_I
PANTHERiPTHR21049 PTHR21049, 1 hit
PfamiView protein in Pfam
PF04597 Ribophorin_I, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

P04843-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEAPAAGLFL LLLLGTWAPA PGSASSEAPP LINEDVKRTV DLSSHLAKVT
60 70 80 90 100
AEVVLAHLGG GSTSRATSFL LALEPELEAR LAHLGVQVKG EDEEENNLEV
110 120 130 140 150
RETKIKGKSG RFFTVKLPVA LDPGAKISVI VETVYTHVLH PYPTQITQSE
160 170 180 190 200
KQFVVFEGNH YFYSPYPTKT QTMRVKLASR NVESYTKLGN PTRSEDLLDY
210 220 230 240 250
GPFRDVPAYS QDTFKVHYEN NSPFLTITSM TRVIEVSHWG NIAVEENVDL
260 270 280 290 300
KHTGAVLKGP FSRYDYQRQP DSGISSIRSF KTILPAAAQD VYYRDEIGNV
310 320 330 340 350
STSHLLILDD SVEMEIRPRF PLFGGWKTHY IVGYNLPSYE YLYNLGDQYA
360 370 380 390 400
LKMRFVDHVF DEQVIDSLTV KIILPEGAKN IEIDSPYEIS RAPDELHYTY
410 420 430 440 450
LDTFGRPVIV AYKKNLVEQH IQDIVVHYTF NKVLMLQEPL LVVAAFYILF
460 470 480 490 500
FTVIIYVRLD FSITKDPAAE ARMKVACITE QVLTLVNKRI GLYRHFDETV
510 520 530 540 550
NRYKQSRDIS TLNSGKKSLE TEHKALTSEI ALLQSRLKTE GSDLCDRVSE
560 570 580 590 600
MQKLDAQVKE LVLKSAVEAE RLVAGKLKKD TYIENEKLIS GKRQELVTKI

DHILDAL
Length:607
Mass (Da):68,569
Last modified:August 13, 1987 - v1
Checksum:iA2351A9CFABAEB6C
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B7Z4L4B7Z4L4_HUMAN
Dolichyl-diphosphooligosaccharide--...
RPN1
435Annotation score:
F8WF32F8WF32_HUMAN
Dolichyl-diphosphooligosaccharide--...
RPN1
121Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00281 mRNA Translation: CAA68392.1
AK312369 mRNA Translation: BAG35287.1
CR749284 mRNA Translation: CAH18139.1
CH471052 Genomic DNA Translation: EAW79306.1
CH471052 Genomic DNA Translation: EAW79307.1
CH471052 Genomic DNA Translation: EAW79308.1
BC010839 mRNA Translation: AAH10839.1
CCDSiCCDS3051.1
PIRiA26168
RefSeqiNP_002941.1, NM_002950.3
UniGeneiHs.518244
Hs.603636

Genome annotation databases

EnsembliENST00000296255; ENSP00000296255; ENSG00000163902
GeneIDi6184
KEGGihsa:6184
UCSCiuc003ekr.2 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00281 mRNA Translation: CAA68392.1
AK312369 mRNA Translation: BAG35287.1
CR749284 mRNA Translation: CAH18139.1
CH471052 Genomic DNA Translation: EAW79306.1
CH471052 Genomic DNA Translation: EAW79307.1
CH471052 Genomic DNA Translation: EAW79308.1
BC010839 mRNA Translation: AAH10839.1
CCDSiCCDS3051.1
PIRiA26168
RefSeqiNP_002941.1, NM_002950.3
UniGeneiHs.518244
Hs.603636

3D structure databases

ProteinModelPortaliP04843
SMRiP04843
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112099, 160 interactors
CORUMiP04843
DIPiDIP-38152N
IntActiP04843, 55 interactors
MINTiP04843
STRINGi9606.ENSP00000296255

PTM databases

GlyConnecti1186
iPTMnetiP04843
PhosphoSitePlusiP04843
SwissPalmiP04843

Polymorphism and mutation databases

BioMutaiRPN1
DMDMi132559

Proteomic databases

EPDiP04843
MaxQBiP04843
PaxDbiP04843
PeptideAtlasiP04843
PRIDEiP04843
ProteomicsDBi51749
TopDownProteomicsiP04843

Protocols and materials databases

DNASUi6184
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000296255; ENSP00000296255; ENSG00000163902
GeneIDi6184
KEGGihsa:6184
UCSCiuc003ekr.2 human

Organism-specific databases

CTDi6184
DisGeNETi6184
EuPathDBiHostDB:ENSG00000163902.11
GeneCardsiRPN1
HGNCiHGNC:10381 RPN1
HPAiCAB009748
HPA026828
HPA051520
MalaCardsiRPN1
MIMi180470 gene
neXtProtiNX_P04843
OpenTargetsiENSG00000163902
Orphaneti402020 'Acute myeloid leukemia with inv3(p21;q26.2) or t(3;3)(p21;q26.2)'
PharmGKBiPA34777
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2291 Eukaryota
ENOG410XQVZ LUCA
GeneTreeiENSGT00390000009630
HOGENOMiHOG000247000
HOVERGENiHBG012864
InParanoidiP04843
KOiK12666
OMAiRYPIFGG
OrthoDBiEOG091G05K5
PhylomeDBiP04843
TreeFamiTF312988

Enzyme and pathway databases

UniPathwayi
UPA00378

ReactomeiR-HSA-1799339 SRP-dependent cotranslational protein targeting to membrane
R-HSA-446203 Asparagine N-linked glycosylation
SIGNORiP04843

Miscellaneous databases

ChiTaRSiRPN1 human
GeneWikiiRPN1
GenomeRNAii6184
PROiPR:P04843
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000163902 Expressed in 232 organ(s), highest expression level in corpus epididymis
CleanExiHS_RPN1
ExpressionAtlasiP04843 baseline and differential
GenevisibleiP04843 HS

Family and domain databases

InterProiView protein in InterPro
IPR007676 Ribophorin_I
PANTHERiPTHR21049 PTHR21049, 1 hit
PfamiView protein in Pfam
PF04597 Ribophorin_I, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRPN1_HUMAN
AccessioniPrimary (citable) accession number: P04843
Secondary accession number(s): B2R5Z0, D3DNB6, Q68DT1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: October 10, 2018
This is version 182 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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