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Protein

Cyclomaltodextrin glucanotransferase

Gene

cgtM

Organism
Paenibacillus macerans (Bacillus macerans)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

Cyclizes part of a (1->4)-alpha-D-glucan chain by formation of a (1->4)-alpha-D-glucosidic bond.

Cofactori

Ca2+By similarityNote: Binds 2 calcium ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi54Calcium 1By similarity1
Metal bindingi59Calcium 1By similarity1
Metal bindingi60Calcium 1By similarity1
Metal bindingi78Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi80Calcium 1By similarity1
Metal bindingi166Calcium 2By similarity1
Binding sitei167SubstrateBy similarity1
Metal bindingi217Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi226Calcium 2By similarity1
Binding sitei254SubstrateBy similarity1
Active sitei256NucleophileBy similarity1
Metal bindingi260Calcium 2; via carbonyl oxygenBy similarity1
Active sitei285Proton donorBy similarity1
Binding sitei355SubstrateBy similarity1
Sitei356Transition state stabilizerBy similarity1
Binding sitei399SubstrateBy similarity1
Binding sitei403SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
LigandCalcium, Metal-binding

Enzyme and pathway databases

BRENDAi2.4.1.19 670

Protein family/group databases

CAZyiCBM20 Carbohydrate-Binding Module Family 20
GH13 Glycoside Hydrolase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclomaltodextrin glucanotransferase (EC:2.4.1.19)
Alternative name(s):
Cyclodextrin-glycosyltransferase
Short name:
CGTase
Gene namesi
Name:cgtM
OrganismiPaenibacillus macerans (Bacillus macerans)
Taxonomic identifieri44252 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesPaenibacillaceaePaenibacillus

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Add BLAST27
ChainiPRO_000000143428 – 714Cyclomaltodextrin glucanotransferaseAdd BLAST687

Interactioni

Subunit structurei

Monomer.

Structurei

Secondary structure

1714
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP04830
SMRiP04830
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini527 – 607IPT/TIGAdd BLAST81
Domaini609 – 714CBM20PROSITE-ProRule annotationAdd BLAST106

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni28 – 165A1Add BLAST138
Regioni127 – 128Substrate bindingBy similarity2
Regioni166 – 229BAdd BLAST64
Regioni220 – 223Substrate bindingBy similarity4
Regioni230 – 434A2Add BLAST205
Regioni259 – 260Substrate bindingBy similarity2
Regioni435 – 523CAdd BLAST89
Regioni524 – 610DAdd BLAST87
Regioni611 – 714EAdd BLAST104

Domaini

May consist of two protein domains: the one in the N-terminal side cleaves the alpha-1,4-glucosidic bond in starch, and the other in the C-terminal side catalyzes other activities, including the reconstitution of an alpha-1,4-glucosidic linkage for cyclizing the maltooligosaccharide produced.

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.40.10, 2 hits
2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR031319 A-amylase_C
IPR006046 Alpha_amylase
IPR013784 Carb-bd-like_fold
IPR002044 CBM_fam20
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
IPR013783 Ig-like_fold
IPR014756 Ig_E-set
IPR002909 IPT_dom
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF00686 CBM_20, 1 hit
PF01833 TIG, 1 hit
PRINTSiPR00110 ALPHAAMYLASE
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SM00632 Aamy_C, 1 hit
SM01065 CBM_2, 1 hit
SUPFAMiSSF49452 SSF49452, 1 hit
SSF51445 SSF51445, 1 hit
SSF81296 SSF81296, 1 hit
PROSITEiView protein in PROSITE
PS51166 CBM20, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04830-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKSRYKRLTS LALSLSMALG ISLPAWASPD TSVDNKVNFS TDVIYQIVTD
60 70 80 90 100
RFADGDRTNN PAGDAFSGDR SNLKLYFGGD WQGIIDKIND GYLTGMGVTA
110 120 130 140 150
LWISQPVENI TSVIKYSGVN NTSYHGYWAR DFKQTNDAFG DFADFQNLID
160 170 180 190 200
TAHAHNIKVV IDFAPNHTSP ADRDNPGFAE NGGMYDNGSL LGAYSNDTAG
210 220 230 240 250
LFHHNGGTDF STIEDGIYKN LYDLADINHN NNAMDAYFKS AIDLWLGMGV
260 270 280 290 300
DGIRFDAVKH MPFGWQKSFV SSIYGGDHPV FTFGEWYLGA DQTDGDNIKF
310 320 330 340 350
ANESGMNLLD FEYAQEVREV FRDKTETMKD LYEVLASTES QYDYINNMVT
360 370 380 390 400
FIDNHDMDRF QVAGSGTRAT EQALALTLTS RGVPAIYYGT EQYMTGDGDP
410 420 430 440 450
NNRAMMTSFN TGTTAYKVIQ ALAPLRKSNP AIAYGTTTER WVNNDVLIIE
460 470 480 490 500
RKFGSSAALV AINRNSSAAY PISGLLSSLP AGTYSDVLNG LLNGNSITVG
510 520 530 540 550
SGGAVTNFTL AAGGTAVWQY TAPETSPAIG NVGPTMGQPG NIVTIDGRGF
560 570 580 590 600
GGTAGTVYFG TTAVTGSGIV SWEDTQIKAV IPKVAAGKTG VSVKTSSGTA
610 620 630 640 650
SNTFKSFNVL TGDQVTVRFL VNQANTNYGT NVYLVGNAAE LGSWDPNKAI
660 670 680 690 700
GPMYNQVIAK YPSWYYDVSV PAGTKLDFKF IKKGGGTVTW EGGGNHTYTT
710
PASGVGTVTV DWQN
Length:714
Mass (Da):76,960
Last modified:February 1, 1994 - v2
Checksum:iF8A9AE794D2F3054
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti152 – 174AHAHN…PADRD → LTLITSRSDRLRPQPHVSGR AGT in AAA22298 (PubMed:3011735).CuratedAdd BLAST23
Sequence conflicti183 – 184GM → AL in AAA22298 (PubMed:3011735).Curated2
Sequence conflicti260 – 261HM → QY in AAA22298 (PubMed:3011735).Curated2
Sequence conflicti643S → T in AAA22298 (PubMed:3011735).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59045 Genomic DNA Translation: CAA41773.1
M12777 Genomic DNA Translation: AAA22298.1
PIRiS31281 ALBSGR

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59045 Genomic DNA Translation: CAA41773.1
M12777 Genomic DNA Translation: AAA22298.1
PIRiS31281 ALBSGR

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3WMSX-ray2.30A28-714[»]
4JCLX-ray1.70A28-714[»]
ProteinModelPortaliP04830
SMRiP04830
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiCBM20 Carbohydrate-Binding Module Family 20
GH13 Glycoside Hydrolase Family 13

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi2.4.1.19 670

Family and domain databases

Gene3Di2.60.40.10, 2 hits
2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR031319 A-amylase_C
IPR006046 Alpha_amylase
IPR013784 Carb-bd-like_fold
IPR002044 CBM_fam20
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
IPR013783 Ig-like_fold
IPR014756 Ig_E-set
IPR002909 IPT_dom
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF00686 CBM_20, 1 hit
PF01833 TIG, 1 hit
PRINTSiPR00110 ALPHAAMYLASE
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SM00632 Aamy_C, 1 hit
SM01065 CBM_2, 1 hit
SUPFAMiSSF49452 SSF49452, 1 hit
SSF51445 SSF51445, 1 hit
SSF81296 SSF81296, 1 hit
PROSITEiView protein in PROSITE
PS51166 CBM20, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCDGT1_PAEMA
AccessioniPrimary (citable) accession number: P04830
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: February 1, 1994
Last modified: February 28, 2018
This is version 121 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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