TYMS

Functionⁱ

Contributes to the de novo mitochondrial thymidylate biosynthesis pathway.1 Publication

Catalytic activityⁱ

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.

Pathwayⁱ: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	50	dUMPBy similarity	1
Active siteⁱ	195	NucleophileBy similarity	1
Binding siteⁱ	218	5,10-methylenetetrahydrofolateBy similarity	1
Binding siteⁱ	226	dUMPBy similarity	1
Binding siteⁱ	312	5,10-methylenetetrahydrofolate; via carbonyl oxygenBy similarity	1

Regions

Feature key	Position(s)	DescriptionActions	Length
Nucleotide bindingⁱ	175 – 176	dUMP; shared with dimeric partnerBy similarity	2
Nucleotide bindingⁱ	215 – 218	dUMPBy similarity	4
Nucleotide bindingⁱ	256 – 258	dUMPBy similarity	3

GO - Molecular functionⁱ

cofactor binding Source: Ensembl
folic acid binding
Source: BHF-UCL
Inferred by curatorⁱ
- 15093541
nucleotide binding Source: Ensembl
protein homodimerization activity Source: Ensembl
sequence-specific mRNA binding
Source: CAFA
Inferred from direct assayⁱ
- 1924359
thymidylate synthase activity
Source: CAFA
Inferred from direct assayⁱ
- 11278511
- 8845352
translation repressor activity, mRNA regulatory element binding
Source: CAFA
Inferred from direct assayⁱ
- 1924359

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

aging Source: Ensembl
cartilage development Source: Ensembl
circadian rhythm Source: Ensembl
developmental growth Source: Ensembl
DNA biosynthetic process
Source: BHF-UCL
Inferred by curatorⁱ
- 15093541
dTMP biosynthetic process
Source: CAFA
Inferred from direct assayⁱ
- 11278511
- 8845352
dTTP biosynthetic process Source: UniProtKB-UniPathway
dUMP metabolic process Source: Ensembl
immortalization of host cell by virus Source: Ensembl
intestinal epithelial cell maturation Source: Ensembl
liver regeneration Source: Ensembl
negative regulation of translation
Source: CAFA
Inferred from direct assayⁱ
- 1924359
nucleobase-containing small molecule interconversion Source: Reactome
regulation of transcription involved in G1/S transition of mitotic cell cycle Source: Reactome
response to cytokine Source: Ensembl
response to ethanol Source: Ensembl
response to folic acid Source: Ensembl
response to glucocorticoid Source: Ensembl
response to organophosphorus
Source: UniProtKB
Inferred from expression patternⁱ
- 16079077
response to progesterone Source: Ensembl
response to vitamin A Source: Ensembl
tetrahydrofolate interconversion
Source: BHF-UCL
Inferred from direct assayⁱ
- 15093541
uracil metabolic process Source: Ensembl

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Methyltransferase, Transferase
Biological process	Nucleotide biosynthesis

Enzyme and pathway databases

BioCycⁱ	MetaCyc:HS11096-MONOMER
BRENDAⁱ	2.1.1.45 2681
Reactomeⁱ	R-HSA-499943 Interconversion of nucleotide di- and triphosphates R-HSA-539107 Activation of E2F1 target genes at G1/S
SABIO-RKⁱ	P04818
SIGNORⁱ	P04818
UniPathwayⁱ	UPA00575

Protein family/group databases

MoonProtⁱ	P04818
UniLectinⁱ	P04818

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Thymidylate synthase (EC:2.1.1.45) Short name: TS Short name: TSase
Gene namesⁱ	Name:TYMS Synonyms:TS ORF Names:OK/SW-cl.29
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 18

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000176890.15
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:12441 TYMS
MIMⁱ	188350 gene
neXtProtⁱ	NX_P04818

Keywords - Cellular componentⁱ

Cytoplasm, Membrane, Mitochondrion, Mitochondrion inner membrane, Nucleus

Pathology & Biotechⁱ

Organism-specific databases

DisGeNET More...DisGeNETⁱ	7298
MalaCards human disease database More...MalaCardsⁱ	TYMS
Open Targets More...OpenTargetsⁱ	ENSG00000176890
Orphanetⁱ	240839 5-fluorouracil toxicity
PharmGKBⁱ	PA359

Chemistry databases

ChEMBLⁱ	CHEMBL1952
Drug and drug target database More...DrugBankⁱ	DB03800 2'-deoxyuridylic acid DB05308 ANX-510 DB01101 Capecitabine DB00322 Floxuridine DB00544 Fluorouracil DB00441 Gemcitabine DB00563 Methotrexate DB08478 N-[2-chloro-5-(trifluoromethyl)phenyl]imidodicarbonimidic diamide DB05116 NB1011 DB05457 OSI-7904L DB00642 Pemetrexed DB06813 Pralatrexate DB00293 Raltitrexed DB01643 Thymidine-5'-Phosphate DB00432 Trifluridine DB00440 Trimethoprim
IUPHAR/BPS Guide to PHARMACOLOGY More...GuidetoPHARMACOLOGYⁱ	2642

Polymorphism and mutation databases

BioMutaⁱ	TYMS
DMDMⁱ	136611

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Initiator methionineⁱ		Removed2 Publications
ChainⁱPRO_0000140901	2 – 313	Thymidylate synthaseAdd BLAST		312

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	114	PhosphoserineCombined sources	1
Cross-linkⁱ	287	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linkⁱ	292	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linkⁱ	308	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Keywords - PTMⁱ

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDⁱ	P04818
PaxDbⁱ	P04818
PeptideAtlas More...PeptideAtlasⁱ	P04818
PRIDEⁱ	P04818
ProteomicsDBⁱ	51747
TopDownProteomicsⁱ	P04818-1 [P04818-1]

PTM databases

iPTMnetⁱ	P04818
PhosphoSitePlusⁱ	P04818

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSG00000176890 Expressed in 204 organ(s), highest expression level in liver
CleanExⁱ	HS_TYMS
ExpressionAtlasⁱ	P04818 baseline and differential
Genevisibleⁱ	P04818 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	CAB002784

HPAⁱ

CAB002784

Interactionⁱ

Subunit structureⁱ

Homodimer.

GO - Molecular functionⁱ

protein homodimerization activity Source: Ensembl

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	113149, 56 interactors
IntActⁱ	P04818, 9 interactors
Molecular INTeraction database More...MINTⁱ	P04818
STRINGⁱ	9606.ENSP00000315644

Chemistry databases

BindingDBⁱ

P04818

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	30 – 43	Combined sources	14
Beta strandⁱ	45 – 48	Combined sources	4
Beta strandⁱ	50 – 52	Combined sources	3
Beta strandⁱ	54 – 66	Combined sources	13
Beta strandⁱ	68 – 70	Combined sources	3
Turnⁱ	75 – 78	Combined sources	4
Helixⁱ	81 – 92	Combined sources	12
Helixⁱ	98 – 101	Combined sources	4
Turnⁱ	103 – 105	Combined sources	3
Turnⁱ	108 – 110	Combined sources	3
Helixⁱ	111 – 113	Combined sources	3
Helixⁱ	117 – 120	Combined sources	4
Helixⁱ	123 – 127	Combined sources	5
Turnⁱ	132 – 134	Combined sources	3
Helixⁱ	136 – 141	Combined sources	6
Beta strandⁱ	149 – 151	Combined sources	3
Beta strandⁱ	156 – 158	Combined sources	3
Helixⁱ	160 – 170	Combined sources	11
Beta strandⁱ	178 – 181	Combined sources	4
Helixⁱ	184 – 186	Combined sources	3
Turnⁱ	187 – 189	Combined sources	3
Beta strandⁱ	190 – 192	Combined sources	3
Beta strandⁱ	196 – 204	Combined sources	9
Beta strandⁱ	207 – 218	Combined sources	12
Turnⁱ	219 – 221	Combined sources	3
Helixⁱ	222 – 241	Combined sources	20
Beta strandⁱ	244 – 258	Combined sources	15
Helixⁱ	259 – 261	Combined sources	3
Helixⁱ	262 – 268	Combined sources	7
Beta strandⁱ	278 – 281	Combined sources	4
Helixⁱ	288 – 290	Combined sources	3
Helixⁱ	293 – 295	Combined sources	3
Beta strandⁱ	296 – 300	Combined sources	5

Show more details

3D structure databases

Database of protein disorder More...DisProtⁱ	DP00073
ProteinModelPortalⁱ	P04818
SMRⁱ	P04818
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	P04818

EvolutionaryTraceⁱ

P04818

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the thymidylate synthase family.Curated

Phylogenomic databases

eggNOGⁱ	KOG0673 Eukaryota COG0207 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000014786
HOGENOMⁱ	HOG000257899
HOVERGENⁱ	HBG001934
InParanoidⁱ	P04818
KEGG Orthology (KO) More...KOⁱ	K00560
OMAⁱ	KQYLDLC
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0CV0
PhylomeDBⁱ	P04818
TreeFamⁱ	TF353027

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00351 TS_Pyrimidine_HMase, 1 hit
Gene3Dⁱ	3.30.572.10, 1 hit
HAMAPⁱ	MF_00008 Thymidy_synth_bact, 1 hit
InterProⁱ	View protein in InterPro IPR023451 Thymidate_synth/dCMP_Mease IPR036926 Thymidate_synth/dCMP_Mease_sf IPR000398 Thymidylate_synthase IPR020940 Thymidylate_synthase_AS
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00303 Thymidylat_synt, 1 hit
PRINTSⁱ	PR00108 THYMDSNTHASE
SUPFAMⁱ	SSF55831 SSF55831, 1 hit
TIGRFAMsⁱ	TIGR03284 thym_sym, 1 hit
PROSITEⁱ	View protein in PROSITE PS00091 THYMIDYLATE_SYNTHASE, 1 hit

Sequences (3)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsⁱ produced by alternative splicing. Align Add to basket

Isoform 1 (identifier: P04818-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPVAGSELPR RPLPPAAQER DAEPRPPHGE LQYLGQIQHI LRCGVRKDDR 
        60         70         80         90        100
TGTGTLSVFG MQARYSLRDE FPLLTTKRVF WKGVLEELLW FIKGSTNAKE 
       110        120        130        140        150
LSSKGVKIWD ANGSRDFLDS LGFSTREEGD LGPVYGFQWR HFGAEYRDME 
       160        170        180        190        200
SDYSGQGVDQ LQRVIDTIKT NPDDRRIIMC AWNPRDLPLM ALPPCHALCQ 
       210        220        230        240        250
FYVVNSELSC QLYQRSGDMG LGVPFNIASY ALLTYMIAHI TGLKPGDFIH 
       260        270        280        290        300
TLGDAHIYLN HIEPLKIQLQ REPRPFPKLR ILRKVEKIDD FKAEDFQIEG 
       310 
YNPHPTIKME MAV

Length:313

Mass (Da):35,716

Last modified:January 23, 2007 - v3

Checksum:ⁱ148D377F19915B6A

GO

Isoform 2 (identifier: P04818-2) [UniParc]FASTA Add to basket

Also known as: delta4

The sequence of this isoform differs from the canonical sequence as follows:
152-185: Missing.

« Hide

        10         20         30         40         50
MPVAGSELPR RPLPPAAQER DAEPRPPHGE LQYLGQIQHI LRCGVRKDDR 
        60         70         80         90        100
TGTGTLSVFG MQARYSLRDE FPLLTTKRVF WKGVLEELLW FIKGSTNAKE 
       110        120        130        140        150
LSSKGVKIWD ANGSRDFLDS LGFSTREEGD LGPVYGFQWR HFGAEYRDME 
       160        170        180        190        200
SDLPLMALPP CHALCQFYVV NSELSCQLYQ RSGDMGLGVP FNIASYALLT 
       210        220        230        240        250
YMIAHITGLK PGDFIHTLGD AHIYLNHIEP LKIQLQREPR PFPKLRILRK 
       260        270 
VEKIDDFKAE DFQIEGYNPH PTIKMEMAV

Note: Expressed both in normal and cancerous tissues.

Show »

Length:279

Mass (Da):31,759

Checksum:ⁱ10B3A53DE10AB763

GO

Isoform 3 (identifier: P04818-3) [UniParc]FASTA Add to basket

Also known as: delta2+3

The sequence of this isoform differs from the canonical sequence as follows:
69-151: Missing.

« Hide

        10         20         30         40         50
MPVAGSELPR RPLPPAAQER DAEPRPPHGE LQYLGQIQHI LRCGVRKDDR 
        60         70         80         90        100
TGTGTLSVFG MQARYSLRDY SGQGVDQLQR VIDTIKTNPD DRRIIMCAWN 
       110        120        130        140        150
PRDLPLMALP PCHALCQFYV VNSELSCQLY QRSGDMGLGV PFNIASYALL 
       160        170        180        190        200
TYMIAHITGL KPGDFIHTLG DAHIYLNHIE PLKIQLQREP RPFPKLRILR 
       210        220        230
KVEKIDDFKA EDFQIEGYNP HPTIKMEMAV

Note: Expressed only in cancerous tissues.

Show »

Length:230

Mass (Da):26,140

Checksum:ⁱD09F8316A504A02A

GO

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_047745	69 – 151	Missing in isoform 3. 1 PublicationAdd BLAST		83
Alternative sequenceⁱVSP_047746	152 – 185	Missing in isoform 2. 1 PublicationAdd BLAST		34

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X02308 mRNA Translation: CAA26178.1 D00596 Genomic DNA Translation: BAA00472.1 AB077207 mRNA Translation: BAB83676.1 AB077208 mRNA Translation: BAB83677.1 AB062290 mRNA Translation: BAB93473.1 AP001178 Genomic DNA No translation available. CH471113 Genomic DNA Translation: EAX01716.1 CH471113 Genomic DNA Translation: EAX01720.1 BC002567 mRNA Translation: AAH02567.1 BC013919 mRNA Translation: AAH13919.1 BC083512 mRNA Translation: AAH83512.1 D00517 Genomic DNA Translation: BAA00404.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS11821.1 [P04818-1] CCDS86658.1 [P04818-2] CCDS86659.1 [P04818-3]
PIRⁱ	A23047 YXHUT
NCBI Reference Sequences More...RefSeqⁱ	NP_001062.1, NM_001071.2 [P04818-1]
UniGeneⁱ	Hs.369762

Genome annotation databases

Ensemblⁱ	ENST00000323224; ENSP00000314727; ENSG00000176890 [P04818-2] ENST00000323250; ENSP00000314902; ENSG00000176890 [P04818-3] ENST00000323274; ENSP00000315644; ENSG00000176890 [P04818-1]
GeneIDⁱ	7298
KEGGⁱ	hsa:7298
UCSC genome browser More...UCSCⁱ	uc010dkb.2 human [P04818-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P04818	Thymidylate synthetase		HUMAN		313	UniRef100_P04818
UPI0000683214		HUMAN		355
UPI0000683213		HUMAN		288

Protein	Similar proteins		Species	Score	Length	Source
P04818	Thymidylate synthetase		HUMAN		313	UniRef90_P04818
Uncharacterized protein		PONAB		338
TYMS isoform 1		PONAB		313
UPI0000683214		HUMAN		355
UPI0000683213		HUMAN		288
+5
P04818-2	thymidylate synthase isoform X2		ODORO		284	UniRef90_P04818-2
Uncharacterized protein		FELCA		282
thymidylate synthase isoform X2		Enhydra lutris kenyoni		282
Uncharacterized protein		COLAP		279
Uncharacterized protein		RHIBE		279
+23
P04818-3	thymidylate synthase isoform X3		Enhydra lutris kenyoni		233	UniRef90_P04818-3
Uncharacterized protein		FELCA		233
TYMS isoform 4		PANTR		230
Uncharacterized protein		PANPA		230
Uncharacterized protein		PROCO		230
+2

Protein	Similar proteins		Species	Score	Length	Source
P04818	Thymidylate synthase		BOVIN		354	UniRef50_P04818
Thymidylate synthetase		HUMAN		313
Uncharacterized protein		PONAB		338
Thymidylate synthase (Fragment)		bluefin notho		372
Thymidylate synthase (Fragment)		Nothobranchius pienaari		379
+112
P04818-2	thymidylate synthase isoform X2		ODORO		284	UniRef50_P04818-2
Uncharacterized protein		FELCA		282
thymidylate synthase isoform X2		Enhydra lutris kenyoni		282
Uncharacterized protein		MACMU		279
Uncharacterized protein		RHIRO		279
+24
P04818-3	Uncharacterized protein		FELCA		233	UniRef50_P04818-3
thymidylate synthase isoform X3		Enhydra lutris kenyoni		233
Uncharacterized protein		PROCO		230
TYMS isoform 3		PONAB		230
TYMS isoform 4		PANTR		230
+8

Cross-referencesⁱ

Web resourcesⁱ

SHMPD

The Singapore human mutation and polymorphism database

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X02308 mRNA Translation: CAA26178.1 D00596 Genomic DNA Translation: BAA00472.1 AB077207 mRNA Translation: BAB83676.1 AB077208 mRNA Translation: BAB83677.1 AB062290 mRNA Translation: BAB93473.1 AP001178 Genomic DNA No translation available. CH471113 Genomic DNA Translation: EAX01716.1 CH471113 Genomic DNA Translation: EAX01720.1 BC002567 mRNA Translation: AAH02567.1 BC013919 mRNA Translation: AAH13919.1 BC083512 mRNA Translation: AAH83512.1 D00517 Genomic DNA Translation: BAA00404.1
CCDSⁱ	CCDS11821.1 [P04818-1] CCDS86658.1 [P04818-2] CCDS86659.1 [P04818-3]
PIRⁱ	A23047 YXHUT
RefSeqⁱ	NP_001062.1, NM_001071.2 [P04818-1]
UniGeneⁱ	Hs.369762

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1HVY	X-ray	1.90	A/B/C/D	26-313	[»]
	1HW3	X-ray	2.00	A	1-313	[»]
	1HW4	X-ray	2.06	A	1-313	[»]
	1HZW	X-ray	2.00	A/B	1-313	[»]
	1I00	X-ray	2.50	A/B	1-313	[»]
	1JU6	X-ray	2.89	A/B/C/D	1-313	[»]
	1JUJ	X-ray	3.00	A/B/C/D	1-313	[»]
	1YPV	X-ray	1.80	A	1-313	[»]
	2ONB	X-ray	2.70	A	1-313	[»]
	2RD8	X-ray	2.50	A/B	1-313	[»]
	2RDA	X-ray	2.67	A/B/C/D/E/F	1-313	[»]
	3EAW	X-ray	1.86	X	1-313	[»]
	3EBU	X-ray	2.05	A	1-313	[»]
	3ED7	X-ray	1.56	A	26-313	[»]
	3EDW	X-ray	1.75	X	1-313	[»]
	3EF9	X-ray	3.20	A	1-313	[»]
	3EGY	X-ray	2.18	X	1-313	[»]
	3EHI	X-ray	2.00	X	1-313	[»]
	3EJL	X-ray	3.20	A/B/C/D	1-313	[»]
	3GG5	X-ray	2.77	A/B/C/D	1-313	[»]
	3GH0	X-ray	1.56	A	1-313	[»]
	3GH2	X-ray	1.75	X	1-313	[»]
	3H9K	X-ray	2.65	A/B/C/D/E	1-313	[»]
	3HB8	X-ray	2.74	A/B/C/D/E	1-313	[»]
	3N5E	X-ray	2.26	A/B	1-313	[»]
	3N5G	X-ray	2.27	A	1-313	[»]
	3OB7	X-ray	2.75	A/B/C/D/E	1-313	[»]
	4E28	X-ray	2.30	A	1-313	[»]
	4FGT	X-ray	2.00	A	1-311	[»]
	4G2O	X-ray	2.25	X	1-313	[»]
	4G6W	X-ray	2.30	X	1-313	[»]
	4GD7	X-ray	2.29	A	1-313	[»]
	4GYH	X-ray	3.00	A	1-313	[»]
	4H1I	X-ray	3.10	A/B/C/D	1-313	[»]
	4JEF	X-ray	2.31	A	26-311	[»]
	4KPW	X-ray	2.03	A	1-313	[»]
	4O1U	X-ray	2.26	A/B	1-313	[»]
	4O1X	X-ray	2.32	A/B/C/D	1-313	[»]
	4UP1	X-ray	2.99	A/B/C/D	1-313	[»]
	5HS3	X-ray	3.10	A/B/C/D/E/F	26-313	[»]
	5WRN	X-ray	2.39	A/B/C/D/E/F	26-313	[»]
	5X4W	X-ray	2.10	A	1-313	[»]
	5X4X	X-ray	2.31	A	1-313	[»]
	5X4Y	X-ray	2.20	A	1-313	[»]
	5X5A	X-ray	2.39	A/B/C/D/E/F	26-313	[»]
	5X5D	X-ray	2.00	A/B/C/D/E/F	26-313	[»]
	5X5Q	X-ray	2.79	A/B/C/D/E/F	26-313	[»]
	5X66	X-ray	1.99	A/B/C/D/E/F	26-313	[»]
	5X67	X-ray	2.13	A/B	26-313	[»]
	5X69	X-ray	2.69	A/B/C/D/E/F	26-313	[»]
DisProtⁱ	DP00073
ProteinModelPortalⁱ	P04818
SMRⁱ	P04818
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	113149, 56 interactors
IntActⁱ	P04818, 9 interactors
MINTⁱ	P04818
STRINGⁱ	9606.ENSP00000315644

Chemistry databases

BindingDBⁱ	P04818
ChEMBLⁱ	CHEMBL1952
DrugBankⁱ	DB03800 2'-deoxyuridylic acid DB05308 ANX-510 DB01101 Capecitabine DB00322 Floxuridine DB00544 Fluorouracil DB00441 Gemcitabine DB00563 Methotrexate DB08478 N-[2-chloro-5-(trifluoromethyl)phenyl]imidodicarbonimidic diamide DB05116 NB1011 DB05457 OSI-7904L DB00642 Pemetrexed DB06813 Pralatrexate DB00293 Raltitrexed DB01643 Thymidine-5'-Phosphate DB00432 Trifluridine DB00440 Trimethoprim
GuidetoPHARMACOLOGYⁱ	2642

Protein family/group databases

MoonProtⁱ	P04818
UniLectinⁱ	P04818

PTM databases

iPTMnetⁱ	P04818
PhosphoSitePlusⁱ	P04818

Polymorphism and mutation databases

BioMutaⁱ	TYMS
DMDMⁱ	136611

Proteomic databases

EPDⁱ	P04818
PaxDbⁱ	P04818
PeptideAtlasⁱ	P04818
PRIDEⁱ	P04818
ProteomicsDBⁱ	51747
TopDownProteomicsⁱ	P04818-1 [P04818-1]

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	7298
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000323224; ENSP00000314727; ENSG00000176890 [P04818-2] ENST00000323250; ENSP00000314902; ENSG00000176890 [P04818-3] ENST00000323274; ENSP00000315644; ENSG00000176890 [P04818-1]
GeneIDⁱ	7298
KEGGⁱ	hsa:7298
UCSCⁱ	uc010dkb.2 human [P04818-1]

Organism-specific databases

CTDⁱ	7298
DisGeNETⁱ	7298
EuPathDBⁱ	HostDB:ENSG00000176890.15
GeneCardsⁱ	TYMS
H-InvDBⁱ	HIX0021150
HGNCⁱ	HGNC:12441 TYMS
HPAⁱ	CAB002784
MalaCardsⁱ	TYMS
MIMⁱ	188350 gene
neXtProtⁱ	NX_P04818
OpenTargetsⁱ	ENSG00000176890
Orphanetⁱ	240839 5-fluorouracil toxicity
PharmGKBⁱ	PA359
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG0673 Eukaryota COG0207 LUCA
GeneTreeⁱ	ENSGT00390000014786
HOGENOMⁱ	HOG000257899
HOVERGENⁱ	HBG001934
InParanoidⁱ	P04818
KOⁱ	K00560
OMAⁱ	KQYLDLC
OrthoDBⁱ	EOG091G0CV0
PhylomeDBⁱ	P04818
TreeFamⁱ	TF353027

Enzyme and pathway databases

UniPathwayⁱ	UPA00575
BioCycⁱ	MetaCyc:HS11096-MONOMER
BRENDAⁱ	2.1.1.45 2681
Reactomeⁱ	R-HSA-499943 Interconversion of nucleotide di- and triphosphates R-HSA-539107 Activation of E2F1 target genes at G1/S
SABIO-RKⁱ	P04818
SIGNORⁱ	P04818

Miscellaneous databases

ChiTaRSⁱ	TYMS human
EvolutionaryTraceⁱ	P04818
GeneWikiⁱ	Thymidylate_synthase
GenomeRNAiⁱ	7298
Protein Ontology More...PROⁱ	PR:P04818
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000176890 Expressed in 204 organ(s), highest expression level in liver
CleanExⁱ	HS_TYMS
ExpressionAtlasⁱ	P04818 baseline and differential
Genevisibleⁱ	P04818 HS

Family and domain databases

CDDⁱ	cd00351 TS_Pyrimidine_HMase, 1 hit
Gene3Dⁱ	3.30.572.10, 1 hit
HAMAPⁱ	MF_00008 Thymidy_synth_bact, 1 hit
InterProⁱ	View protein in InterPro IPR023451 Thymidate_synth/dCMP_Mease IPR036926 Thymidate_synth/dCMP_Mease_sf IPR000398 Thymidylate_synthase IPR020940 Thymidylate_synthase_AS
Pfamⁱ	View protein in Pfam PF00303 Thymidylat_synt, 1 hit
PRINTSⁱ	PR00108 THYMDSNTHASE
SUPFAMⁱ	SSF55831 SSF55831, 1 hit
TIGRFAMsⁱ	TIGR03284 thym_sym, 1 hit
PROSITEⁱ	View protein in PROSITE PS00091 THYMIDYLATE_SYNTHASE, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	TYSY_HUMAN
Accessionⁱ	P04818Primary (citable) accession number: P04818 Secondary accession number(s): Q8WYK3, Q8WYK4
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
	Last sequence update:	January 23, 2007
	Last modified:	October 10, 2018
	This is version 203 of the entry and version 3 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
PATHWAY comments
Index of metabolic and biosynthesis pathways
Human chromosome 18
Human chromosome 18: entries, gene names and cross-references to MIM
PDB cross-references
Index of Protein Data Bank (PDB) cross-references

UniProtKB

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UniRef

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Supporting data

UniProtKB - P04818 (TYSY_HUMAN)

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Thymidylate synthase

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: dTTP biosynthesis

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Mitochondrion

Nucleus

Other locations

Cytosol

Mitochondrion

Nucleus

Other locations

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Organism-specific databases

Chemistry databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

Chemistry databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

<p>This subsection of the <a href="http://www.uniprot.org/manual/cross_references_section">Cross-references</a> section provides links to various web resources that are relevant for a specific protein.<p><a href='/help/web_resource' target='_top'>More...</a></p>Web resourcesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

Protein family/group databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

Pathwayⁱ: dTTP biosynthesis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Web resourcesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ