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Protein

Hexokinase-2

Gene

HXK2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Main glucose phosphorylating enzyme. May play a regulatory role in both induction and repression of gene expression by glucose.

Miscellaneous

In yeast there are three glucose-phosphorylating isoenzymes, designated hexokinase I, II and glucokinase.
Present with 114000 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + D-hexose = ADP + D-hexose 6-phosphate.

Activity regulationi

Subject to allosteric control. Substrate inhibition by ATP.

Pathwayi: hexose metabolism

This protein is involved in the pathway hexose metabolism, which is part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the pathway hexose metabolism and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei111ATPSequence analysis1
Binding sitei158Substrate; via carbonyl oxygenBy similarity1
Binding sitei237SubstrateBy similarity1
Binding sitei269SubstrateBy similarity1
Binding sitei302SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi86 – 91ATPBy similarity6
Nucleotide bindingi307 – 308ATPBy similarity2
Nucleotide bindingi344 – 348ATPBy similarity5
Nucleotide bindingi419 – 423ATPBy similarity5

GO - Molecular functioni

GO - Biological processi

  • cellular glucose homeostasis Source: GO_Central
  • fructose import Source: SGD
  • fructose metabolic process Source: SGD
  • glucose import Source: SGD
  • glucose metabolic process Source: SGD
  • glycolytic process Source: SGD
  • mannose metabolic process Source: SGD
  • regulation of transcription by glucose Source: SGD
  • replicative cell aging Source: SGD

Keywordsi

Molecular functionAllosteric enzyme, Kinase, Transferase
Biological processGlycolysis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YGL253W-MONOMER
BRENDAi2.7.1.1 984
SABIO-RKiP04807
UniPathwayi
UPA00242

Protein family/group databases

MoonProtiP04807

Names & Taxonomyi

Protein namesi
Recommended name:
Hexokinase-2 (EC:2.7.1.1)
Alternative name(s):
Hexokinase PII
Hexokinase-B
Gene namesi
Name:HXK2
Synonyms:HEX1, HKB
Ordered Locus Names:YGL253W
ORF Names:NRB486
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL253W
SGDiS000003222 HXK2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001976022 – 486Hexokinase-2Add BLAST485

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei15PhosphoserineCombined sources2 Publications1
Modified residuei38PhosphothreonineCombined sources1
Modified residuei158PhosphoserineCombined sources1 Publication1
Modified residuei245PhosphoserineCombined sources1
Modified residuei272PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP04807
PaxDbiP04807
PRIDEiP04807
TopDownProteomicsiP04807

2D gel databases

SWISS-2DPAGEiP04807

PTM databases

CarbonylDBiP04807
iPTMnetiP04807

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi33026, 280 interactors
DIPiDIP-2380N
IntActiP04807, 16 interactors
MINTiP04807
STRINGi4932.YGL253W

Structurei

Secondary structure

1486
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP04807
SMRiP04807
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04807

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini21 – 469HexokinasePROSITE-ProRule annotationAdd BLAST449

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni75 – 209Hexokinase small subdomainPROSITE-ProRule annotationAdd BLAST135
Regioni175 – 176Substrate bindingBy similarity2
Regioni210 – 458Hexokinase large subdomainPROSITE-ProRule annotationAdd BLAST249
Regioni210 – 211Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the hexokinase family.PROSITE-ProRule annotationCurated

Phylogenomic databases

GeneTreeiENSGT00910000145864
HOGENOMiHOG000162670
InParanoidiP04807
KOiK00844
OMAiERQFFRA
OrthoDBiEOG092C2JW4

Family and domain databases

InterProiView protein in InterPro
IPR001312 Hexokinase
IPR019807 Hexokinase_BS
IPR022673 Hexokinase_C
IPR022672 Hexokinase_N
PANTHERiPTHR19443 PTHR19443, 1 hit
PfamiView protein in Pfam
PF00349 Hexokinase_1, 1 hit
PF03727 Hexokinase_2, 1 hit
PROSITEiView protein in PROSITE
PS00378 HEXOKINASE_1, 1 hit
PS51748 HEXOKINASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04807-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVHLGPKKPQ ARKGSMADVP KELMQQIENF EKIFTVPTET LQAVTKHFIS
60 70 80 90 100
ELEKGLSKKG GNIPMIPGWV MDFPTGKESG DFLAIDLGGT NLRVVLVKLG
110 120 130 140 150
GDRTFDTTQS KYRLPDAMRT TQNPDELWEF IADSLKAFID EQFPQGISEP
160 170 180 190 200
IPLGFTFSFP ASQNKINEGI LQRWTKGFDI PNIENHDVVP MLQKQITKRN
210 220 230 240 250
IPIEVVALIN DTTGTLVASY YTDPETKMGV IFGTGVNGAY YDVCSDIEKL
260 270 280 290 300
QGKLSDDIPP SAPMAINCEY GSFDNEHVVL PRTKYDITID EESPRPGQQT
310 320 330 340 350
FEKMSSGYYL GEILRLALMD MYKQGFIFKN QDLSKFDKPF VMDTSYPARI
360 370 380 390 400
EEDPFENLED TDDLFQNEFG INTTVQERKL IRRLSELIGA RAARLSVCGI
410 420 430 440 450
AAICQKRGYK TGHIAADGSV YNRYPGFKEK AANALKDIYG WTQTSLDDYP
460 470 480
IKIVPAEDGS GAGAAVIAAL AQKRIAEGKS VGIIGA
Length:486
Mass (Da):53,942
Last modified:January 23, 2007 - v4
Checksum:iD55FF3F8992B2FEF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti29N → I in CAA27203 (PubMed:3003701).Curated1
Sequence conflicti33I → N in AAA34697 (PubMed:3905511).Curated1
Sequence conflicti33I → N in AAA34699 (PubMed:3905511).Curated1
Sequence conflicti61G → V in CAA27203 (PubMed:3003701).Curated1
Sequence conflicti197T → S in CAA27203 (PubMed:3003701).Curated1
Sequence conflicti202P → H in AAA34699 (PubMed:3905511).Curated1
Sequence conflicti421 – 422YN → ST in AAA34697 (PubMed:3905511).Curated2
Sequence conflicti421 – 422YN → ST in AAA34699 (PubMed:3905511).Curated2
Sequence conflicti444 – 445TS → PH in AAA34697 (PubMed:3905511).Curated2
Sequence conflicti444 – 445TS → PH in AAA34699 (PubMed:3905511).Curated2
Sequence conflicti453I → V in AAA34697 (PubMed:3905511).Curated1
Sequence conflicti453I → V in AAA34699 (PubMed:3905511).Curated1
Sequence conflicti462A → P in AAA34697 (PubMed:3905511).Curated1
Sequence conflicti462A → P in AAA34699 (PubMed:3905511).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03483 Genomic DNA Translation: CAA27203.1
M11181 Genomic DNA Translation: AAA34697.1
M14411 mRNA Translation: AAA34699.1
X94357 Genomic DNA Translation: CAA64134.1
Z72775 Genomic DNA Translation: CAA96973.1
X67787 Genomic DNA Translation: CAA48003.1
BK006941 Genomic DNA Translation: DAA07866.1
PIRiS61608 KIBYHB
RefSeqiNP_011261.1, NM_001181119.1

Genome annotation databases

EnsemblFungiiYGL253W; YGL253W; YGL253W
GeneIDi852639
KEGGisce:YGL253W

Similar proteinsi

Cross-referencesi

Web resourcesi

Worthington enzyme manual

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03483 Genomic DNA Translation: CAA27203.1
M11181 Genomic DNA Translation: AAA34697.1
M14411 mRNA Translation: AAA34699.1
X94357 Genomic DNA Translation: CAA64134.1
Z72775 Genomic DNA Translation: CAA96973.1
X67787 Genomic DNA Translation: CAA48003.1
BK006941 Genomic DNA Translation: DAA07866.1
PIRiS61608 KIBYHB
RefSeqiNP_011261.1, NM_001181119.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IG8X-ray2.20A1-486[»]
2YHXX-ray2.10A152-471[»]
5UWTX-ray2.34D14-36[»]
ProteinModelPortaliP04807
SMRiP04807
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33026, 280 interactors
DIPiDIP-2380N
IntActiP04807, 16 interactors
MINTiP04807
STRINGi4932.YGL253W

Protein family/group databases

MoonProtiP04807

PTM databases

CarbonylDBiP04807
iPTMnetiP04807

2D gel databases

SWISS-2DPAGEiP04807

Proteomic databases

MaxQBiP04807
PaxDbiP04807
PRIDEiP04807
TopDownProteomicsiP04807

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL253W; YGL253W; YGL253W
GeneIDi852639
KEGGisce:YGL253W

Organism-specific databases

EuPathDBiFungiDB:YGL253W
SGDiS000003222 HXK2

Phylogenomic databases

GeneTreeiENSGT00910000145864
HOGENOMiHOG000162670
InParanoidiP04807
KOiK00844
OMAiERQFFRA
OrthoDBiEOG092C2JW4

Enzyme and pathway databases

UniPathwayi
UPA00242

BioCyciYEAST:YGL253W-MONOMER
BRENDAi2.7.1.1 984
SABIO-RKiP04807

Miscellaneous databases

EvolutionaryTraceiP04807
PROiPR:P04807

Family and domain databases

InterProiView protein in InterPro
IPR001312 Hexokinase
IPR019807 Hexokinase_BS
IPR022673 Hexokinase_C
IPR022672 Hexokinase_N
PANTHERiPTHR19443 PTHR19443, 1 hit
PfamiView protein in Pfam
PF00349 Hexokinase_1, 1 hit
PF03727 Hexokinase_2, 1 hit
PROSITEiView protein in PROSITE
PS00378 HEXOKINASE_1, 1 hit
PS51748 HEXOKINASE_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiHXKB_YEAST
AccessioniPrimary (citable) accession number: P04807
Secondary accession number(s): D6VV82, Q05838
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: January 23, 2007
Last modified: September 12, 2018
This is version 193 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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