UniProtKB - P04789 (TPIS_TRYBB)
Protein
Triosephosphate isomerase, glycosomal
Gene
N/A
Organism
Trypanosoma brucei brucei
Status
Functioni
Miscellaneous
The enzyme contains a high proportion of positively-charged residues in beta-barrels V and VII (compared to the homologous regions in other triose isomerase sequences). Since 2 clusters of positive charges located at precise distances on the molecular surface are common to 4 glycosomal enzymes, it has been speculated that this might represent a signal for entry into glycosomes (PubMed:3015595).1 Publication
Catalytic activityi
- EC:5.3.1.1
: gluconeogenesis Pathwayi
This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.
Pathwayi: glycolysis
This protein is involved in step 1 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate.Proteins known to be involved in this subpathway in this organism are:
- Triosephosphate isomerase, glycosomal
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate, the pathway glycolysis and in Carbohydrate degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 11 | Substrate | 1 | |
Binding sitei | 13 | Substrate | 1 | |
Active sitei | 95 | Electrophile | 1 | |
Active sitei | 167 | Proton acceptor | 1 |
GO - Molecular functioni
- triose-phosphate isomerase activity Source: GeneDB
GO - Biological processi
- gluconeogenesis Source: UniProtKB-UniPathway
- glycolytic process Source: GeneDB
Keywordsi
Molecular function | Isomerase |
Biological process | Gluconeogenesis, Glycolysis |
Enzyme and pathway databases
BRENDAi | 5.3.1.1, 6519 |
SABIO-RKi | P04789 |
UniPathwayi | UPA00109;UER00189 UPA00138 |
Names & Taxonomyi
Protein namesi | Recommended name: Triosephosphate isomerase, glycosomal (EC:5.3.1.1)Short name: TIM Short name: Triose-phosphate isomerase |
Organismi | Trypanosoma brucei brucei |
Taxonomic identifieri | 5702 [NCBI] |
Taxonomic lineagei | Eukaryota › Discoba › Euglenozoa › Kinetoplastea › Metakinetoplastina › Trypanosomatida › Trypanosomatidae › Trypanosoma › |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000090142 | 1 – 250 | Triosephosphate isomerase, glycosomalAdd BLAST | 250 |
Proteomic databases
PRIDEi | P04789 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P04789 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P04789 |
Family & Domainsi
Sequence similaritiesi
Belongs to the triosephosphate isomerase family.Curated
Family and domain databases
CDDi | cd00311, TIM, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
HAMAPi | MF_00147_B, TIM_B, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR035990, TIM_sf IPR022896, TrioseP_Isoase_bac/euk IPR000652, Triosephosphate_isomerase IPR020861, Triosephosphate_isomerase_AS |
PANTHERi | PTHR21139, PTHR21139, 1 hit |
Pfami | View protein in Pfam PF00121, TIM, 1 hit |
SUPFAMi | SSF51351, SSF51351, 1 hit |
TIGRFAMsi | TIGR00419, tim, 1 hit |
PROSITEi | View protein in PROSITE PS00171, TIM_1, 1 hit PS51440, TIM_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P04789-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSKPQPIAAA NWKCNGSQQS LSELIDLFNS TSINHDVQCV VASTFVHLAM
60 70 80 90 100
TKERLSHPKF VIAAQNAIAK SGAFTGEVSL PILKDFGVNW IVLGHSERRA
110 120 130 140 150
YYGETNEIVA DKVAAAVASG FMVIACIGET LQERESGRTA VVVLTQIAAI
160 170 180 190 200
AKKLKKADWA KVVIAYEPVW AIGTGKVATP QQAQEAHALI RSWVSSKIGA
210 220 230 240 250
DVAGELRILY GGSVNGKNAR TLYQQRDVNG FLVGGASLKP EFVDIIKATQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X03921 Genomic DNA Translation: CAA27559.1 |
Genome annotation databases
GeneDBi | Tb927.11.5520:pep |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X03921 Genomic DNA Translation: CAA27559.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1AG1 | X-ray | 2.36 | O/T | 1-250 | [»] | |
1DKW | X-ray | 2.65 | A/B | 2-250 | [»] | |
1IIG | X-ray | 2.60 | A/B | 1-250 | [»] | |
1IIH | X-ray | 2.20 | A/B | 1-250 | [»] | |
1KV5 | X-ray | 1.65 | A/B | 1-250 | [»] | |
1ML1 | X-ray | 2.60 | A/C/E/G/I/K | 1-250 | [»] | |
1MSS | X-ray | 2.40 | A/B | 1-250 | [»] | |
1MTM | model | - | A | 1-250 | [»] | |
1TPD | X-ray | 2.10 | A/B | 1-250 | [»] | |
1TPE | X-ray | 2.10 | A | 1-250 | [»] | |
1TPF | X-ray | 1.80 | A/B | 1-250 | [»] | |
1TRD | X-ray | 2.50 | A/B | 1-250 | [»] | |
1TRI | X-ray | 2.40 | A | 1-250 | [»] | |
1TSI | X-ray | 2.84 | A/B | 1-250 | [»] | |
1TTI | X-ray | 2.40 | A | 1-250 | [»] | |
1TTJ | X-ray | 2.40 | A | 1-250 | [»] | |
2J24 | X-ray | 2.10 | A/B | 1-250 | [»] | |
2J27 | X-ray | 1.15 | A/B | 1-250 | [»] | |
2V0T | X-ray | 2.20 | A/B/C/D/E/F/G/H | 1-250 | [»] | |
2V2C | X-ray | 1.89 | A | 1-250 | [»] | |
2V2D | X-ray | 2.30 | A | 1-250 | [»] | |
2V2H | X-ray | 1.18 | A/B/C | 1-250 | [»] | |
2V5L | X-ray | 2.40 | A/B | 1-250 | [»] | |
2VEI | X-ray | 1.89 | A/B/C | 2-250 | [»] | |
2VEK | X-ray | 1.60 | A/B | 2-250 | [»] | |
2VEL | X-ray | 2.30 | A/B | 2-250 | [»] | |
2VEM | X-ray | 2.20 | A/B | 2-250 | [»] | |
2VEN | X-ray | 2.00 | A/B | 2-250 | [»] | |
2WSQ | X-ray | 2.10 | A/B/C/D | 2-67 | [»] | |
A/B/C/D | 84-250 | [»] | ||||
2WSR | X-ray | 1.65 | A | 2-67 | [»] | |
A | 84-250 | [»] | ||||
2X16 | X-ray | 2.13 | A/B | 2-250 | [»] | |
2X1R | X-ray | 1.98 | A/B | 2-250 | [»] | |
2X1S | X-ray | 1.93 | A/B | 2-250 | [»] | |
2X1T | X-ray | 1.83 | A/B | 2-250 | [»] | |
2X1U | X-ray | 1.84 | A/B | 2-250 | [»] | |
2X2G | X-ray | 1.90 | A/B | 2-250 | [»] | |
2Y6Z | X-ray | 2.60 | A | 1-250 | [»] | |
2Y70 | X-ray | 2.30 | A/B/C/D | 1-250 | [»] | |
3Q37 | X-ray | 1.65 | A/B/C/D | 2-35 | [»] | |
A/B/C/D | 92-119 | [»] | ||||
3TIM | X-ray | 2.80 | A/B | 1-250 | [»] | |
4JEQ | X-ray | 2.30 | A/B/C/D/E/F/G/H/I/J/K/L | 1-250 | [»] | |
4PC8 | X-ray | 1.55 | A | 1-250 | [»] | |
4PCF | X-ray | 2.71 | A/B/C | 1-250 | [»] | |
4TIM | X-ray | 2.40 | A/B | 1-250 | [»] | |
5I3F | X-ray | 1.72 | A/B/C/D | 1-250 | [»] | |
5I3G | X-ray | 1.96 | A/B/C/D | 1-250 | [»] | |
5I3H | X-ray | 2.25 | A/B | 1-250 | [»] | |
5I3I | X-ray | 2.20 | A/B/C/D | 1-250 | [»] | |
5I3J | X-ray | 1.80 | A/B | 1-250 | [»] | |
5I3K | X-ray | 2.21 | A/B/C/D | 1-250 | [»] | |
5TIM | X-ray | 1.83 | A/B | 1-250 | [»] | |
6TIM | X-ray | 2.20 | A/B | 1-250 | [»] | |
SMRi | P04789 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Chemistry databases
BindingDBi | P04789 |
Proteomic databases
PRIDEi | P04789 |
Genome annotation databases
GeneDBi | Tb927.11.5520:pep |
Enzyme and pathway databases
UniPathwayi | UPA00109;UER00189 UPA00138 |
BRENDAi | 5.3.1.1, 6519 |
SABIO-RKi | P04789 |
Miscellaneous databases
EvolutionaryTracei | P04789 |
Family and domain databases
CDDi | cd00311, TIM, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
HAMAPi | MF_00147_B, TIM_B, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR035990, TIM_sf IPR022896, TrioseP_Isoase_bac/euk IPR000652, Triosephosphate_isomerase IPR020861, Triosephosphate_isomerase_AS |
PANTHERi | PTHR21139, PTHR21139, 1 hit |
Pfami | View protein in Pfam PF00121, TIM, 1 hit |
SUPFAMi | SSF51351, SSF51351, 1 hit |
TIGRFAMsi | TIGR00419, tim, 1 hit |
PROSITEi | View protein in PROSITE PS00171, TIM_1, 1 hit PS51440, TIM_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TPIS_TRYBB | |
Accessioni | P04789Primary (citable) accession number: P04789 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 13, 1987 |
Last sequence update: | February 1, 1995 | |
Last modified: | June 17, 2020 | |
This is version 145 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencingDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families