UniProtKB - P04756 (ACHA_MOUSE)
Protein
Acetylcholine receptor subunit alpha
Gene
Chrna1
Organism
Mus musculus (Mouse)
Status
Functioni
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
GO - Molecular functioni
- acetylcholine-gated cation-selective channel activity Source: MGI
- neurotransmitter receptor activity Source: GO_Central
- transmembrane signaling receptor activity Source: InterPro
- transmitter-gated ion channel activity involved in regulation of postsynaptic membrane potential Source: MGI
GO - Biological processi
- cation transport Source: MGI
- chemical synaptic transmission Source: GO_Central
- ion transmembrane transport Source: GO_Central
- muscle cell cellular homeostasis Source: MGI
- musculoskeletal movement Source: MGI
- nervous system process Source: GO_Central
- neuromuscular junction development Source: MGI
- neuromuscular process Source: MGI
- neuromuscular synaptic transmission Source: MGI
- neuronal action potential Source: MGI
- neuron cellular homeostasis Source: MGI
- regulation of membrane potential Source: MGI
- signal transduction Source: GO_Central
- skeletal muscle contraction Source: MGI
- skeletal muscle tissue growth Source: MGI
- synaptic transmission, cholinergic Source: GOC
Keywordsi
Molecular function | Ion channel, Ligand-gated ion channel, Receptor |
Biological process | Ion transport, Transport |
Enzyme and pathway databases
Reactomei | R-MMU-629594, Highly calcium permeable postsynaptic nicotinic acetylcholine receptors R-MMU-629597, Highly calcium permeable nicotinic acetylcholine receptors |
Names & Taxonomyi
Protein namesi | Recommended name: Acetylcholine receptor subunit alpha |
Gene namesi | Name:Chrna1 Synonyms:Acra |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:87885, Chrna1 |
Subcellular locationi
Plasma Membrane
- acetylcholine-gated channel complex Source: MGI
- integral component of plasma membrane Source: GO_Central
- integral component of postsynaptic specialization membrane Source: SynGO
- plasma membrane Source: MGI
Other locations
- cell surface Source: MGI
- membrane Source: MGI
- neuromuscular junction Source: MGI
- neuron projection Source: GO_Central
- synapse Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 21 – 230 | ExtracellularAdd BLAST | 210 | |
Transmembranei | 231 – 255 | HelicalAdd BLAST | 25 | |
Transmembranei | 263 – 281 | HelicalAdd BLAST | 19 | |
Transmembranei | 297 – 316 | HelicalAdd BLAST | 20 | |
Topological domaini | 317 – 428 | CytoplasmicAdd BLAST | 112 | |
Transmembranei | 429 – 447 | HelicalAdd BLAST | 19 |
Keywords - Cellular componenti
Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, SynapsePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 20 | Add BLAST | 20 | |
ChainiPRO_0000000306 | 21 – 457 | Acetylcholine receptor subunit alphaAdd BLAST | 437 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 148 ↔ 162 | 1 Publication | ||
Glycosylationi | 161 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Disulfide bondi | 212 ↔ 213 | Associated with receptor activation1 Publication |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PaxDbi | P04756 |
PRIDEi | P04756 |
PTM databases
GlyGeni | P04756, 1 site |
iPTMneti | P04756 |
PhosphoSitePlusi | P04756 |
SwissPalmi | P04756 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000027107, Expressed in tarsal region and 119 other tissues |
ExpressionAtlasi | P04756, baseline and differential |
Genevisiblei | P04756, MM |
Interactioni
Subunit structurei
Pentamer of two alpha chains, and one each of the beta, delta, and gamma (in immature muscle) or epsilon (in mature muscle) chains (PubMed:17643119). The muscle heteropentamer composed of alpha-1, beta-1, delta, epsilon subunits interacts with the alpha-conotoxin ImII (By similarity).
By similarity1 PublicationProtein-protein interaction databases
BioGRIDi | 197931, 4 interactors |
ComplexPortali | CPX-252, Muscle-type nicotinic acetylcholine receptor complex, alpha1-beta1-delta-gamma CPX-257, Muscle-type nicotinic acetylcholine receptor complex, alpha1-beta1-delta-epsilon |
DIPi | DIP-59594N |
IntActi | P04756, 2 interactors |
MINTi | P04756 |
STRINGi | 10090.ENSMUSP00000028515 |
Chemistry databases
BindingDBi | P04756 |
Miscellaneous databases
RNActi | P04756, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P04756 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P04756 |
Family & Domainsi
Sequence similaritiesi
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-1/CHRNA1 sub-subfamily. [View classification]Curated
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3645, Eukaryota |
GeneTreei | ENSGT00940000156851 |
HOGENOMi | CLU_018074_1_0_1 |
InParanoidi | P04756 |
OMAi | STHIMPE |
OrthoDBi | 381858at2759 |
PhylomeDBi | P04756 |
TreeFami | TF315605 |
Family and domain databases
Gene3Di | 2.70.170.10, 1 hit |
InterProi | View protein in InterPro IPR006202, Neur_chan_lig-bd IPR036734, Neur_chan_lig-bd_sf IPR006201, Neur_channel IPR036719, Neuro-gated_channel_TM_sf IPR006029, Neurotrans-gated_channel_TM IPR018000, Neurotransmitter_ion_chnl_CS IPR002394, Nicotinic_acetylcholine_rcpt |
PANTHERi | PTHR18945, PTHR18945, 1 hit |
Pfami | View protein in Pfam PF02931, Neur_chan_LBD, 1 hit PF02932, Neur_chan_memb, 1 hit |
PRINTSi | PR00254, NICOTINICR PR00252, NRIONCHANNEL |
SUPFAMi | SSF63712, SSF63712, 1 hit SSF90112, SSF90112, 1 hit |
TIGRFAMsi | TIGR00860, LIC, 1 hit |
PROSITEi | View protein in PROSITE PS00236, NEUROTR_ION_CHANNEL, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P04756-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MELSTVLLLL GLCSAGLVLG SEHETRLVAK LFEDYSSVVR PVEDHREIVQ
60 70 80 90 100
VTVGLQLIQL INVDEVNQIV TTNVRLKQQW VDYNLKWNPD DYGGVKKIHI
110 120 130 140 150
PSEKIWRPDV VLYNNADGDF AIVKFTKVLL DYTGHITWTP PAIFKSYCEI
160 170 180 190 200
IVTHFPFDEQ NCSMKLGTWT YDGSVVAINP ESDQPDLSNF MESGEWVIKE
210 220 230 240 250
ARGWKHWVFY SCCPTTPYLD ITYHFVMQRL PLYFIVNVII PCLLFSFLTS
260 270 280 290 300
LVFYLPTDSG EKMTLSISVL LSLTVFLLVI VELIPSTSSA VPLIGKYMLF
310 320 330 340 350
TMVFVIASII ITVIVINTHH RSPSTHIMPE WVRKVFIDTI PNIMFFSTMK
360 370 380 390 400
RPSRDKQEKR IFTEDIDISD ISGKPGPPPM GFHSPLIKHP EVKSAIEGVK
410 420 430 440 450
YIAETMKSDQ ESNNAAEEWK YVAMVMDHIL LGVFMLVCLI GTLAVFAGRL
IELHQQG
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 13 | C → S in AAB53942 (Ref. 2) Curated | 1 | |
Sequence conflicti | 13 | C → S no nucleotide entry (PubMed:2993547).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X03986 mRNA Translation: CAA27624.1 M17640 mRNA Translation: AAB53942.1 AK029177 mRNA Translation: BAC26337.1 |
CCDSi | CCDS16132.1 |
PIRi | A24383 I49458 |
RefSeqi | NP_031415.2, NM_007389.5 |
Genome annotation databases
Ensembli | ENSMUST00000028515; ENSMUSP00000028515; ENSMUSG00000027107 |
GeneIDi | 11435 |
KEGGi | mmu:11435 |
UCSCi | uc008kcy.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X03986 mRNA Translation: CAA27624.1 M17640 mRNA Translation: AAB53942.1 AK029177 mRNA Translation: BAC26337.1 |
CCDSi | CCDS16132.1 |
PIRi | A24383 I49458 |
RefSeqi | NP_031415.2, NM_007389.5 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2QC1 | X-ray | 1.94 | B | 21-231 | [»] | |
5HBV | X-ray | 2.70 | B | 22-231 | [»] | |
SMRi | P04756 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 197931, 4 interactors |
ComplexPortali | CPX-252, Muscle-type nicotinic acetylcholine receptor complex, alpha1-beta1-delta-gamma CPX-257, Muscle-type nicotinic acetylcholine receptor complex, alpha1-beta1-delta-epsilon |
DIPi | DIP-59594N |
IntActi | P04756, 2 interactors |
MINTi | P04756 |
STRINGi | 10090.ENSMUSP00000028515 |
Chemistry databases
BindingDBi | P04756 |
ChEMBLi | CHEMBL3038460 CHEMBL3137264 |
PTM databases
GlyGeni | P04756, 1 site |
iPTMneti | P04756 |
PhosphoSitePlusi | P04756 |
SwissPalmi | P04756 |
Proteomic databases
PaxDbi | P04756 |
PRIDEi | P04756 |
Protocols and materials databases
ABCDi | P04756, 4 sequenced antibodies |
Antibodypediai | 19487, 412 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000028515; ENSMUSP00000028515; ENSMUSG00000027107 |
GeneIDi | 11435 |
KEGGi | mmu:11435 |
UCSCi | uc008kcy.2, mouse |
Organism-specific databases
CTDi | 1134 |
MGIi | MGI:87885, Chrna1 |
Phylogenomic databases
eggNOGi | KOG3645, Eukaryota |
GeneTreei | ENSGT00940000156851 |
HOGENOMi | CLU_018074_1_0_1 |
InParanoidi | P04756 |
OMAi | STHIMPE |
OrthoDBi | 381858at2759 |
PhylomeDBi | P04756 |
TreeFami | TF315605 |
Enzyme and pathway databases
Reactomei | R-MMU-629594, Highly calcium permeable postsynaptic nicotinic acetylcholine receptors R-MMU-629597, Highly calcium permeable nicotinic acetylcholine receptors |
Miscellaneous databases
BioGRID-ORCSi | 11435, 2 hits in 18 CRISPR screens |
EvolutionaryTracei | P04756 |
PROi | PR:P04756 |
RNActi | P04756, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000027107, Expressed in tarsal region and 119 other tissues |
ExpressionAtlasi | P04756, baseline and differential |
Genevisiblei | P04756, MM |
Family and domain databases
Gene3Di | 2.70.170.10, 1 hit |
InterProi | View protein in InterPro IPR006202, Neur_chan_lig-bd IPR036734, Neur_chan_lig-bd_sf IPR006201, Neur_channel IPR036719, Neuro-gated_channel_TM_sf IPR006029, Neurotrans-gated_channel_TM IPR018000, Neurotransmitter_ion_chnl_CS IPR002394, Nicotinic_acetylcholine_rcpt |
PANTHERi | PTHR18945, PTHR18945, 1 hit |
Pfami | View protein in Pfam PF02931, Neur_chan_LBD, 1 hit PF02932, Neur_chan_memb, 1 hit |
PRINTSi | PR00254, NICOTINICR PR00252, NRIONCHANNEL |
SUPFAMi | SSF63712, SSF63712, 1 hit SSF90112, SSF90112, 1 hit |
TIGRFAMsi | TIGR00860, LIC, 1 hit |
PROSITEi | View protein in PROSITE PS00236, NEUROTR_ION_CHANNEL, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ACHA_MOUSE | |
Accessioni | P04756Primary (citable) accession number: P04756 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 13, 1987 |
Last sequence update: | August 13, 1987 | |
Last modified: | December 2, 2020 | |
This is version 189 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families