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Protein

Pancreatic alpha-amylase

Gene

AMY2A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.4 Publications

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi115CalciumCombined sources4 Publications1
Metal bindingi173Calcium; via carbonyl oxygenCombined sources4 Publications1
Metal bindingi182CalciumCombined sources4 Publications1
Binding sitei210ChlorideCombined sources4 Publications1
Active sitei212Nucleophile3 Publications1
Metal bindingi216Calcium; via carbonyl oxygenCombined sources4 Publications1
Active sitei248Proton donor4 Publications1
Binding sitei313ChlorideCombined sources4 Publications1
Sitei315Transition state stabilizer3 Publications1
Binding sitei352ChlorideCombined sources4 Publications1

GO - Molecular functioni

GO - Biological processi

  • carbohydrate catabolic process Source: UniProtKB
  • carbohydrate metabolic process Source: ProtInc
  • polysaccharide digestion Source: Reactome

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism
LigandCalcium, Chloride, Metal-binding

Enzyme and pathway databases

BRENDAi3.2.1.1 2681
ReactomeiR-HSA-189085 Digestion of dietary carbohydrate
SABIO-RKiP04746

Protein family/group databases

CAZyiGH13 Glycoside Hydrolase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
Pancreatic alpha-amylase (EC:3.2.1.14 Publications)
Short name:
PA
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase
Gene namesi
Name:AMY2A
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000243480.7
HGNCiHGNC:477 AMY2A
MIMi104650 gene
neXtProtiNX_P04746

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi210R → A or Q: Abolishes chloride binding; strongly reduces activity. 1 Publication1
Mutagenesisi212D → A or N: Abolishes activity. 3 Publications1
Mutagenesisi248E → A or Q: Reduces activity. 2 Publications1
Mutagenesisi313N → S: Reduces affinity for chloride; reduces activity. 1 Publication1
Mutagenesisi315D → A or N: Strongly reduces activity. 2 Publications1
Mutagenesisi352R → A: Abolishes chloride binding; has only slight effect on activity. 1 Publication1

Organism-specific databases

DisGeNETi279
OpenTargetsiENSG00000243480
PharmGKBiPA24784

Chemistry databases

ChEMBLiCHEMBL2045
DrugBankiDB03092 5-Hydroxymethyl-Chonduritol
DB03773 6-Deoxy-Alpha-D-Glucose
DB00284 Acarbose
DB03971 Acarbose Derived Hexasaccharide
DB02379 Beta-D-Glucose
DB00702 Icodextrin
DB00491 Miglitol
DB03088 Pyroglutamic Acid

Polymorphism and mutation databases

BioMutaiAMY2A
DMDMi113803

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 15Add BLAST15
ChainiPRO_000000139716 – 511Pancreatic alpha-amylaseAdd BLAST496

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei16Pyrrolidone carboxylic acid2 Publications1
Disulfide bondi43 ↔ 101Combined sources4 Publications
Disulfide bondi85 ↔ 130Combined sources4 Publications
Disulfide bondi156 ↔ 175Combined sources4 Publications
Disulfide bondi393 ↔ 399Combined sources4 Publications
Disulfide bondi465 ↔ 477Combined sources4 Publications
Glycosylationi476N-linked (GlcNAc...) asparagine3 Publications1

Keywords - PTMi

Disulfide bond, Glycoprotein, Pyrrolidone carboxylic acid

Proteomic databases

MaxQBiP04746
PaxDbiP04746
PeptideAtlasiP04746
PRIDEiP04746
ProteomicsDBi51742

PTM databases

iPTMnetiP04746
PhosphoSitePlusiP04746

Expressioni

Tissue specificityi

Detected in pancreas (at protein level).1 Publication

Gene expression databases

BgeeiENSG00000243480 Expressed in 77 organ(s), highest expression level in body of pancreas
CleanExiHS_AMY2A
ExpressionAtlasiP04746 baseline and differential
GenevisibleiP04746 HS

Organism-specific databases

HPAiCAB045960
HPA045394
HPA045399
HPA046980

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi106776, 10 interactors
IntActiP04746, 3 interactors
STRINGi9606.ENSP00000397582

Chemistry databases

BindingDBiP04746

Structurei

Secondary structure

1511
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP04746
SMRiP04746
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04746

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2212 Eukaryota
COG0366 LUCA
GeneTreeiENSGT00390000002882
HOGENOMiHOG000253313
HOVERGENiHBG000061
InParanoidiP04746
KOiK01176
OMAiWSERYQP
OrthoDBiEOG091G07V6
PhylomeDBiP04746
TreeFamiTF312850

Family and domain databases

Gene3Di2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR006048 A-amylase/branching_C
IPR031319 A-amylase_C
IPR006046 Alpha_amylase
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF02806 Alpha-amylase_C, 1 hit
PRINTSiPR00110 ALPHAAMYLASE
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SM00632 Aamy_C, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: P04746-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MKFFLLLFTI GFCWAQYSPN TQQGRTSIVH LFEWRWVDIA LECERYLAPK
60 70 80 90 100
GFGGVQVSPP NENVAIYNPF RPWWERYQPV SYKLCTRSGN EDEFRNMVTR
110 120 130 140 150
CNNVGVRIYV DAVINHMCGN AVSAGTSSTC GSYFNPGSRD FPAVPYSGWD
160 170 180 190 200
FNDGKCKTGS GDIENYNDAT QVRDCRLTGL LDLALEKDYV RSKIAEYMNH
210 220 230 240 250
LIDIGVAGFR LDASKHMWPG DIKAILDKLH NLNSNWFPAG SKPFIYQEVI
260 270 280 290 300
DLGGEPIKSS DYFGNGRVTE FKYGAKLGTV IRKWNGEKMS YLKNWGEGWG
310 320 330 340 350
FVPSDRALVF VDNHDNQRGH GAGGASILTF WDARLYKMAV GFMLAHPYGF
360 370 380 390 400
TRVMSSYRWP RQFQNGNDVN DWVGPPNNNG VIKEVTINPD TTCGNDWVCE
410 420 430 440 450
HRWRQIRNMV IFRNVVDGQP FTNWYDNGSN QVAFGRGNRG FIVFNNDDWS
460 470 480 490 500
FSLTLQTGLP AGTYCDVISG DKINGNCTGI KIYVSDDGKA HFSISNSAED
510
PFIAIHAESK L
Length:511
Mass (Da):57,707
Last modified:July 1, 1989 - v2
Checksum:iA77B1A34EACB3C2A
GO
Isoform 2 (identifier: P04746-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     250-265: IDLGGEPIKSSDYFGN → HQYLYAYKISSYSLEN
     266-511: Missing.

Note: No experimental confirmation available.
Show »
Length:265
Mass (Da):30,302
Checksum:iEDF2723EC07BF231
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H7BZQ8H7BZQ8_HUMAN
Pancreatic alpha-amylase
AMY2A
209Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_055822250 – 265IDLGG…DYFGN → HQYLYAYKISSYSLEN in isoform 2. 1 PublicationAdd BLAST16
Alternative sequenceiVSP_055823266 – 511Missing in isoform 2. 1 PublicationAdd BLAST246

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18785
, M18714, M18716, M18718, M18720, M18722, M18724, M18726, M18783 Genomic DNA Translation: AAA52280.1
M28443 mRNA Translation: AAA51724.1
BC007060 mRNA Translation: AAH07060.1
BC146997 mRNA Translation: AAI46998.1
M18669 Genomic DNA Translation: AAA51723.1
X07056 Genomic DNA Translation: CAA30099.1
CCDSiCCDS783.1 [P04746-1]
PIRiA29614 ALHUP
RefSeqiNP_000690.1, NM_000699.3 [P04746-1]
UniGeneiHs.654437

Genome annotation databases

EnsembliENST00000414303; ENSP00000397582; ENSG00000243480 [P04746-1]
ENST00000622339; ENSP00000481450; ENSG00000243480 [P04746-1]
GeneIDi279
KEGGihsa:279
UCSCiuc001dut.4 human [P04746-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Web resourcesi

Wikipedia

Amylase entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18785
, M18714, M18716, M18718, M18720, M18722, M18724, M18726, M18783 Genomic DNA Translation: AAA52280.1
M28443 mRNA Translation: AAA51724.1
BC007060 mRNA Translation: AAH07060.1
BC146997 mRNA Translation: AAI46998.1
M18669 Genomic DNA Translation: AAA51723.1
X07056 Genomic DNA Translation: CAA30099.1
CCDSiCCDS783.1 [P04746-1]
PIRiA29614 ALHUP
RefSeqiNP_000690.1, NM_000699.3 [P04746-1]
UniGeneiHs.654437

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1B2YX-ray3.20A16-511[»]
1BSIX-ray2.00A17-511[»]
1CPUX-ray2.00A16-511[»]
1HNYX-ray1.80A17-511[»]
1KB3X-ray2.10A16-511[»]
1KBBX-ray1.90A16-511[»]
1KBKX-ray1.90A16-511[»]
1KGUX-ray2.00A16-511[»]
1KGWX-ray2.10A16-511[»]
1KGXX-ray2.00A16-511[»]
1U2YX-ray1.95A16-511[»]
1U30X-ray1.90A16-511[»]
1U33X-ray1.95A16-511[»]
1XCWX-ray2.00A16-511[»]
1XCXX-ray1.90A16-511[»]
1XD0X-ray2.00A16-511[»]
1XD1X-ray2.20A16-511[»]
1XGZX-ray2.00A16-511[»]
1XH0X-ray2.00A16-511[»]
1XH1X-ray2.03A16-511[»]
1XH2X-ray2.20A16-511[»]
2CPUX-ray2.00A17-511[»]
2QMKX-ray2.30A16-511[»]
2QV4X-ray1.97A16-511[»]
3BAIX-ray1.90A16-511[»]
3BAJX-ray2.10A16-511[»]
3BAKX-ray1.90A16-511[»]
3BAWX-ray2.00A16-511[»]
3BAXX-ray1.90A16-511[»]
3BAYX-ray1.99A16-511[»]
3CPUX-ray2.00A17-511[»]
3IJ7X-ray2.00A17-511[»]
3IJ8X-ray1.43A17-511[»]
3IJ9X-ray1.85A17-511[»]
3OLDX-ray2.00A16-511[»]
3OLEX-ray1.55A16-511[»]
3OLGX-ray2.30A16-511[»]
3OLIX-ray1.50A16-511[»]
4GQQX-ray1.35A17-511[»]
4GQRX-ray1.20A17-511[»]
4W93X-ray1.35A17-511[»]
4X9YX-ray1.07A17-511[»]
5E0FX-ray1.40A17-511[»]
5EMYX-ray1.23A17-511[»]
5KEZX-ray1.83A17-511[»]
5TD4X-ray2.30A17-511[»]
5U3AX-ray0.95A16-511[»]
5VA9X-ray2.55A/B16-511[»]
ProteinModelPortaliP04746
SMRiP04746
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi106776, 10 interactors
IntActiP04746, 3 interactors
STRINGi9606.ENSP00000397582

Chemistry databases

BindingDBiP04746
ChEMBLiCHEMBL2045
DrugBankiDB03092 5-Hydroxymethyl-Chonduritol
DB03773 6-Deoxy-Alpha-D-Glucose
DB00284 Acarbose
DB03971 Acarbose Derived Hexasaccharide
DB02379 Beta-D-Glucose
DB00702 Icodextrin
DB00491 Miglitol
DB03088 Pyroglutamic Acid

Protein family/group databases

CAZyiGH13 Glycoside Hydrolase Family 13

PTM databases

iPTMnetiP04746
PhosphoSitePlusiP04746

Polymorphism and mutation databases

BioMutaiAMY2A
DMDMi113803

Proteomic databases

MaxQBiP04746
PaxDbiP04746
PeptideAtlasiP04746
PRIDEiP04746
ProteomicsDBi51742

Protocols and materials databases

DNASUi279
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000414303; ENSP00000397582; ENSG00000243480 [P04746-1]
ENST00000622339; ENSP00000481450; ENSG00000243480 [P04746-1]
GeneIDi279
KEGGihsa:279
UCSCiuc001dut.4 human [P04746-1]

Organism-specific databases

CTDi279
DisGeNETi279
EuPathDBiHostDB:ENSG00000243480.7
GeneCardsiAMY2A
HGNCiHGNC:477 AMY2A
HPAiCAB045960
HPA045394
HPA045399
HPA046980
MIMi104650 gene
neXtProtiNX_P04746
OpenTargetsiENSG00000243480
PharmGKBiPA24784
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2212 Eukaryota
COG0366 LUCA
GeneTreeiENSGT00390000002882
HOGENOMiHOG000253313
HOVERGENiHBG000061
InParanoidiP04746
KOiK01176
OMAiWSERYQP
OrthoDBiEOG091G07V6
PhylomeDBiP04746
TreeFamiTF312850

Enzyme and pathway databases

BRENDAi3.2.1.1 2681
ReactomeiR-HSA-189085 Digestion of dietary carbohydrate
SABIO-RKiP04746

Miscellaneous databases

EvolutionaryTraceiP04746
GeneWikiiAMY2A
GenomeRNAii279
PROiPR:P04746
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000243480 Expressed in 77 organ(s), highest expression level in body of pancreas
CleanExiHS_AMY2A
ExpressionAtlasiP04746 baseline and differential
GenevisibleiP04746 HS

Family and domain databases

Gene3Di2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR006048 A-amylase/branching_C
IPR031319 A-amylase_C
IPR006046 Alpha_amylase
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF02806 Alpha-amylase_C, 1 hit
PRINTSiPR00110 ALPHAAMYLASE
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SM00632 Aamy_C, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAMYP_HUMAN
AccessioniPrimary (citable) accession number: P04746
Secondary accession number(s): B9EJG1, Q9UBH3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: July 1, 1989
Last modified: November 7, 2018
This is version 199 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
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