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Protein

Alpha-amylase 1

Gene

AMY1A

more
Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.1 Publication
Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.By similarity

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi115CalciumCombined sources2 Publications1
Metal bindingi173Calcium; via carbonyl oxygenCombined sources2 Publications1
Metal bindingi182CalciumCombined sources2 Publications1
Binding sitei210ChlorideCombined sources2 Publications1
Active sitei212Nucleophile1
Metal bindingi216Calcium; via carbonyl oxygenCombined sources2 Publications1
Active sitei248Proton donor1
Binding sitei313ChlorideCombined sources2 Publications1
Sitei315Transition state stabilizerBy similarity1
Binding sitei352ChlorideCombined sources2 Publications1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism
LigandCalcium, Chloride, Metal-binding

Enzyme and pathway databases

BRENDAi3.2.1.1 2681
ReactomeiR-HSA-189085 Digestion of dietary carbohydrate

Protein family/group databases

CAZyiGH13 Glycoside Hydrolase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase 1 (EC:3.2.1.11 Publication)
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase 1
Salivary alpha-amylase
Gene namesi
Name:AMY1A
Synonyms:AMY1
AND
Name:AMY1B
Synonyms:AMY1
AND
Name:AMY1C
Synonyms:AMY1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000174876.16
HostDB:ENSG00000187733.6
HostDB:ENSG00000237763.9
HGNCiHGNC:474 AMY1A
HGNC:475 AMY1B
HGNC:476 AMY1C
MIMi104700 gene
104701 gene
104702 gene
neXtProtiNX_P04745

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi276
277
278
OpenTargetsiENSG00000187733
PharmGKBiPA24783

Chemistry databases

ChEMBLiCHEMBL2478
DrugBankiDB03092 5-Hydroxymethyl-Chonduritol
DB02379 Beta-D-Glucose
DB03088 Pyroglutamic Acid
DB04237 Tris(Hydroxyethyl)Aminomethane

Polymorphism and mutation databases

BioMutaiAMY1A
DMDMi1351933

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 15Sequence analysisAdd BLAST15
ChainiPRO_000000140116 – 511Alpha-amylase 1Add BLAST496

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei16Pyrrolidone carboxylic acidBy similarity1
Disulfide bondi43 ↔ 101Combined sources2 Publications
Disulfide bondi85 ↔ 130Combined sources2 Publications
Disulfide bondi156 ↔ 175Combined sources2 Publications
Modified residuei365Deamidated asparagine; partial1 Publication1
Disulfide bondi393 ↔ 399Combined sources2 Publications
Modified residuei427Deamidated asparagine; partial; alternate1 Publication1
Glycosylationi427N-linked (GlcNAc...) asparagine2 Publications1
Disulfide bondi465 ↔ 477Combined sources2 Publications
Modified residuei474Deamidated asparagine; partial1 Publication1
Glycosylationi476N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, Pyrrolidone carboxylic acid

Proteomic databases

MaxQBiP04745
PaxDbiP04745
PeptideAtlasiP04745
PRIDEiP04745
ProteomicsDBi12608
51741

PTM databases

GlyConnecti28
iPTMnetiP04745
PhosphoSitePlusiP04745
SwissPalmiP04745
UniCarbKBiP04745

Expressioni

Gene expression databases

BgeeiENSG00000174876 Expressed in 50 organ(s), highest expression level in anatomical system
CleanExiHS_AMY1A
ExpressionAtlasiP04745 baseline and differential
GenevisibleiP04745 HS

Organism-specific databases

HPAiCAB004310
HPA045394
HPA045399
HPA046980

Interactioni

Subunit structurei

Monomer.

Binary interactionsi

WithEntry#Exp.IntActNotes
MUC7Q8TAX72EBI-738586,EBI-738582

Protein-protein interaction databases

BioGridi106773, 6 interactors
106774, 1 interactor
106775, 41 interactors
IntActiP04745, 3 interactors
STRINGi9606.ENSP00000359100

Chemistry databases

BindingDBiP04745

Structurei

Secondary structure

1511
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP04745
SMRiP04745
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04745

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2212 Eukaryota
COG0366 LUCA
GeneTreeiENSGT00390000002882
HOGENOMiHOG000253313
HOVERGENiHBG000061
InParanoidiP04745
KOiK01176
OMAiHPWWEVY
OrthoDBiEOG09370DCC
PhylomeDBiP04745
TreeFamiTF312850

Family and domain databases

Gene3Di2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR006048 A-amylase/branching_C
IPR031319 A-amylase_C
IPR006046 Alpha_amylase
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF02806 Alpha-amylase_C, 1 hit
PRINTSiPR00110 ALPHAAMYLASE
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SM00632 Aamy_C, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

P04745-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKLFWLLFTI GFCWAQYSSN TQQGRTSIVH LFEWRWVDIA LECERYLAPK
60 70 80 90 100
GFGGVQVSPP NENVAIHNPF RPWWERYQPV SYKLCTRSGN EDEFRNMVTR
110 120 130 140 150
CNNVGVRIYV DAVINHMCGN AVSAGTSSTC GSYFNPGSRD FPAVPYSGWD
160 170 180 190 200
FNDGKCKTGS GDIENYNDAT QVRDCRLSGL LDLALGKDYV RSKIAEYMNH
210 220 230 240 250
LIDIGVAGFR IDASKHMWPG DIKAILDKLH NLNSNWFPEG SKPFIYQEVI
260 270 280 290 300
DLGGEPIKSS DYFGNGRVTE FKYGAKLGTV IRKWNGEKMS YLKNWGEGWG
310 320 330 340 350
FMPSDRALVF VDNHDNQRGH GAGGASILTF WDARLYKMAV GFMLAHPYGF
360 370 380 390 400
TRVMSSYRWP RYFENGKDVN DWVGPPNDNG VTKEVTINPD TTCGNDWVCE
410 420 430 440 450
HRWRQIRNMV NFRNVVDGQP FTNWYDNGSN QVAFGRGNRG FIVFNNDDWT
460 470 480 490 500
FSLTLQTGLP AGTYCDVISG DKINGNCTGI KIYVSDDGKA HFSISNSAED
510
PFIAIHAESK L
Length:511
Mass (Da):57,768
Last modified:February 1, 1996 - v2
Checksum:i7710BCAC83EBE8B2
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q5T085Q5T085_HUMAN
Alpha-amylase 1
AMY1A AMY1B
226Annotation score:
Q5T084Q5T084_HUMAN
Alpha-amylase 1
AMY1A AMY1B
105Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti285N → T in AAA57345 (PubMed:2442579).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18786
, M18715, M18717, M18719, M18721, M18723, M18725, M18727, M18784 Genomic DNA Translation: AAA52279.1
AK292341 mRNA Translation: BAF85030.1
AL513482 Genomic DNA No translation available.
BC063129 mRNA Translation: AAH63129.1
BC069347 mRNA Translation: AAH69347.1
BC069463 mRNA Translation: AAH69463.1
BC092444 mRNA Translation: AAH92444.1
BC132985 mRNA Translation: AAI32986.1
BC132987 mRNA Translation: AAI32988.1
BC132995 mRNA Translation: AAI32996.1
BC132997 mRNA Translation: AAI32998.1
M18671 Genomic DNA Translation: AAA58368.1
M18674 Genomic DNA Translation: AAA16183.2
M19233, M17883 Genomic DNA Translation: AAA57345.1 Different termination.
M17884 Genomic DNA No translation available.
CCDSiCCDS30782.1
CCDS30783.1
CCDS30784.1
PIRiA91543 ALHUS
RefSeqiNP_001008219.1, NM_001008218.1
NP_001008220.1, NM_001008219.2
NP_001008222.1, NM_001008221.1
NP_001333709.1, NM_001346780.1
NP_004029.2, NM_004038.3
XP_011539564.1, XM_011541262.1
XP_016856547.1, XM_017001058.1
UniGeneiHs.599274
Hs.654437
Hs.655232
Hs.662103

Genome annotation databases

EnsembliENST00000330330; ENSP00000330484; ENSG00000174876
ENST00000370079; ENSP00000359096; ENSG00000187733
ENST00000370080; ENSP00000359097; ENSG00000174876
ENST00000370083; ENSP00000359100; ENSG00000237763
GeneIDi276
277
278
KEGGihsa:276
hsa:277
hsa:278
UCSCiuc001duv.4 human

Similar proteinsi

Cross-referencesi

Web resourcesi

Wikipedia

Amylase entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18786
, M18715, M18717, M18719, M18721, M18723, M18725, M18727, M18784 Genomic DNA Translation: AAA52279.1
AK292341 mRNA Translation: BAF85030.1
AL513482 Genomic DNA No translation available.
BC063129 mRNA Translation: AAH63129.1
BC069347 mRNA Translation: AAH69347.1
BC069463 mRNA Translation: AAH69463.1
BC092444 mRNA Translation: AAH92444.1
BC132985 mRNA Translation: AAI32986.1
BC132987 mRNA Translation: AAI32988.1
BC132995 mRNA Translation: AAI32996.1
BC132997 mRNA Translation: AAI32998.1
M18671 Genomic DNA Translation: AAA58368.1
M18674 Genomic DNA Translation: AAA16183.2
M19233, M17883 Genomic DNA Translation: AAA57345.1 Different termination.
M17884 Genomic DNA No translation available.
CCDSiCCDS30782.1
CCDS30783.1
CCDS30784.1
PIRiA91543 ALHUS
RefSeqiNP_001008219.1, NM_001008218.1
NP_001008220.1, NM_001008219.2
NP_001008222.1, NM_001008221.1
NP_001333709.1, NM_001346780.1
NP_004029.2, NM_004038.3
XP_011539564.1, XM_011541262.1
XP_016856547.1, XM_017001058.1
UniGeneiHs.599274
Hs.654437
Hs.655232
Hs.662103

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1C8QX-ray2.30A16-511[»]
1JXJX-ray1.99A16-511[»]
1JXKX-ray1.90A16-511[»]
1MFUX-ray2.00A17-511[»]
1MFVX-ray2.00A17-511[»]
1NM9X-ray2.10A16-511[»]
1Q4NX-ray2.07X16-511[»]
1SMDX-ray1.60A17-511[»]
1XV8X-ray3.00A/B16-511[»]
1Z32X-ray1.60X16-511[»]
3BLKX-ray2.00A16-511[»]
3BLPX-ray1.60X16-511[»]
3DHPX-ray1.50A16-511[»]
ProteinModelPortaliP04745
SMRiP04745
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi106773, 6 interactors
106774, 1 interactor
106775, 41 interactors
IntActiP04745, 3 interactors
STRINGi9606.ENSP00000359100

Chemistry databases

BindingDBiP04745
ChEMBLiCHEMBL2478
DrugBankiDB03092 5-Hydroxymethyl-Chonduritol
DB02379 Beta-D-Glucose
DB03088 Pyroglutamic Acid
DB04237 Tris(Hydroxyethyl)Aminomethane

Protein family/group databases

CAZyiGH13 Glycoside Hydrolase Family 13

PTM databases

GlyConnecti28
iPTMnetiP04745
PhosphoSitePlusiP04745
SwissPalmiP04745
UniCarbKBiP04745

Polymorphism and mutation databases

BioMutaiAMY1A
DMDMi1351933

Proteomic databases

MaxQBiP04745
PaxDbiP04745
PeptideAtlasiP04745
PRIDEiP04745
ProteomicsDBi12608
51741

Protocols and materials databases

DNASUi277
278
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000330330; ENSP00000330484; ENSG00000174876
ENST00000370079; ENSP00000359096; ENSG00000187733
ENST00000370080; ENSP00000359097; ENSG00000174876
ENST00000370083; ENSP00000359100; ENSG00000237763
GeneIDi276
277
278
KEGGihsa:276
hsa:277
hsa:278
UCSCiuc001duv.4 human

Organism-specific databases

CTDi276
277
278
DisGeNETi276
277
278
EuPathDBiHostDB:ENSG00000174876.16
HostDB:ENSG00000187733.6
HostDB:ENSG00000237763.9
GeneCardsiAMY1A
AMY1B
AMY1C
HGNCiHGNC:474 AMY1A
HGNC:475 AMY1B
HGNC:476 AMY1C
HPAiCAB004310
HPA045394
HPA045399
HPA046980
MIMi104700 gene
104701 gene
104702 gene
neXtProtiNX_P04745
OpenTargetsiENSG00000187733
PharmGKBiPA24783
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2212 Eukaryota
COG0366 LUCA
GeneTreeiENSGT00390000002882
HOGENOMiHOG000253313
HOVERGENiHBG000061
InParanoidiP04745
KOiK01176
OMAiHPWWEVY
OrthoDBiEOG09370DCC
PhylomeDBiP04745
TreeFamiTF312850

Enzyme and pathway databases

BRENDAi3.2.1.1 2681
ReactomeiR-HSA-189085 Digestion of dietary carbohydrate

Miscellaneous databases

ChiTaRSiAMY1C human
EvolutionaryTraceiP04745
GeneWikiiAMY1A
PROiPR:P04745
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000174876 Expressed in 50 organ(s), highest expression level in anatomical system
CleanExiHS_AMY1A
ExpressionAtlasiP04745 baseline and differential
GenevisibleiP04745 HS

Family and domain databases

Gene3Di2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR006048 A-amylase/branching_C
IPR031319 A-amylase_C
IPR006046 Alpha_amylase
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF02806 Alpha-amylase_C, 1 hit
PRINTSiPR00110 ALPHAAMYLASE
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SM00632 Aamy_C, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAMY1_HUMAN
AccessioniPrimary (citable) accession number: P04745
Secondary accession number(s): A6NJS5
, A8K8H6, Q13763, Q5T083
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: February 1, 1996
Last modified: November 7, 2018
This is version 212 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
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