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Protein

Pilin

Gene

traA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Propilin is the precursor of the pilus subunit, pilin, that forms conjugative pili, the filamentous surface appendages required for cell-to-cell contact during the earlier stages of bacterial conjugation, and that retract after contact is established. Mature pilin is assembled with the help of TraQ and TraX (PubMed:1464628, PubMed:6090426). Functions as a receptor for CdiA-CT from E.cloacae and E.coli, although it is not clear if this is physiologically relevant (PubMed:24889811).3 Publications

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processConjugation

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Pilin
Alternative name(s):
F-pilin
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:traA
Ordered Locus Names:ECOK12F074
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates if the gene coding for the protein originates from the hydrogenosome, the mitochondrion, the nucleomorph, different plastids or a plasmid. The absence of this section means that the gene is located in one of the main chromosomal element(s).<p><a href='/help/encoded_on' target='_top'>More...</a></p>Encoded oniPlasmid F9 Publications
Plasmid IncFI R3861 Publication
Plasmid IncFI ColV2-K941 Publication
Plasmid IncFI ColVBtrp1 Publication
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular_location_section">'Subcellular location'</a> section describes the subcellular compartment where each non-membrane region of a membrane-spanning protein is found.<p><a href='/help/topo_dom' target='_top'>More...</a></p>Topological domaini1 – 75Periplasmic1 PublicationAdd BLAST75
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular_location_section">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei76 – 96HelicalAdd BLAST21
Topological domaini97 – 100Cytoplasmic1 Publication4
Transmembranei101 – 121HelicalAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane, Secreted

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the ‘Pathology and Biotech’ section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

Loss of sensitivity to toxin CdiA-CT.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi9G → S: No production of pili. 1 Publication1
Mutagenesisi13P → S: No production of pili. 1 Publication1
Mutagenesisi58D → A: No production of propilin or pilin. 1 Publication1
Mutagenesisi68K → A: No production of propilin or pilin. 2 Publications1
Mutagenesisi68K → E: Resistant to phage R17 attachment. 2 Publications1
Mutagenesisi69A → E: No effect on propilin synthesis. No effect on pilus formation. Resistant to phage R17 attachment. 1 Publication1
Mutagenesisi71F → V: No effect on propilin synthesis. Defective pilus tip assembly. 1 Publication1
Mutagenesisi72G → D: No effect on propilin synthesis. No effect on pilus formation. 1 Publication1
Mutagenesisi73K → A or T: No effect on propilin synthesis. No effect on pilus formation. 2 Publications1
Mutagenesisi74D → G: No effect on propilin synthesis, no effect on pilus formation, resistant to phage R17 attachment (Ref.11). About 67% conjugation efficieny, E.coli still sensitive to CdiA-CT toxin (Ref.12). 2 Publications1
Mutagenesisi79K → A: No effect on propilin synthesis. Resistant to phage R17 attachment. 1 Publication1
Mutagenesisi79K → I: No effect on propilin synthesis. Defective pilus tip assembly. 1 Publication1
Mutagenesisi81V → I: Slightly reduced phage R17 attachment, reduced ability to retract. 1 Publication1
Mutagenesisi85E → A or G: No effect on propilin synthesis. Defective pilus tip assembly. 1 Publication1
Mutagenesisi93Y → D: Production of small amounts of propilin and pilin. 1 Publication1
Mutagenesisi97K → A, R or V: No effect on propilin synthesis. No effect on pilus formation. Inability to support R17 phage eclipse. 1 Publication1
Mutagenesisi97K → A: Reduced cleavage of propilin to pilin; when associated with A-100. 1 Publication1
Mutagenesisi98N → A: No effect on propilin synthesis. No effect on pilus formation. 1 Publication1
Mutagenesisi99V → A: No effect on propilin synthesis. No effect on pilus formation. Inability to support R17 phage eclipse. 1 Publication1
Mutagenesisi100K → A, R or T: No effect on propilin synthesis. No effect on pilus formation. Inability to support R17 phage eclipse. Reduced DNA transfer. 1 Publication1
Mutagenesisi100K → A: Reduced cleavage of propilin to pilin; when associated with A-97. 1 Publication1
Mutagenesisi101F → A: No effect on propilin synthesis. No effect on pilus formation. Inability to support R17 phage eclipse. Reduced DNA transfer. 1 Publication1
Mutagenesisi105F → V: No effect on propilin synthesis. No effect on pilus formation. Inability to support R17 phage eclipse. Reduced DNA transfer. 1 Publication1
Mutagenesisi111F → V: No effect on propilin synthesis. No effect on pilus formation. Inability to support R17 phage eclipse. Reduced DNA transfer. 1 Publication1
Mutagenesisi120G → C: Resistant to phage R17, conjugation normal, provides partial resistance to CdiA-CT toxin. 1 Publication1
Mutagenesisi120G → D: Resistant to phages R17 and QB attachment. 1 Publication1
Mutagenesisi121L → S: No effect on propilin synthesis. Defective pilus tip assembly. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/ptm_processing_section">PTM / Processing</a> section describes a propeptide, which is a part of a protein that is cleaved during maturation or activation. Once cleaved, a propeptide generally has no independent biological function.<p><a href='/help/propep' target='_top'>More...</a></p>PropeptideiPRO_00000244871 – 51Leader peptide; cleaved by LepB1 PublicationAdd BLAST51
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_000002448852 – 121PilinAdd BLAST70

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei52N-acetylalanine1 Publication1

Keywords - PTMi

Acetylation

Proteomic databases

PRoteomics IDEntifications database

More...
PRIDEi
P04737

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
P04737

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Monomer. Interacts with itself to form filaments; also interacts with TraQ.3 Publications

Protein-protein interaction databases

Protein interaction database and analysis system

More...
IntActi
P04737, 1 interactor

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

Protein Model Portal of the PSI-Nature Structural Biology Knowledgebase

More...
ProteinModelPortali
P04737

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P04737

Database of comparative protein structure models

More...
ModBasei
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the TraA family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR008873 TraA

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF05513 TraA, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR02758 TraA_TIGR, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

P04737-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNAVLSVQGA SAPVKKKSFF SKFTRLNMLR LARAVIPAAV LMMFFPQLAM
60 70 80 90 100
AAGSSGQDLM ASGNTTVKAT FGKDSSVVKW VVLAEVLVGA VMYMMTKNVK
110 120
FLAGFAIISV FIAVGMAVVG L
Length:121
Mass (Da):12,768
Last modified:August 13, 1987 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iC65EEA3C6E7EF2A2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti115G → V in AAA24910 (PubMed:6090426).Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
K01147 Genomic DNA Translation: AAA24910.1
U01159 Genomic DNA Translation: AAC44177.1
M11322 Genomic DNA Translation: AAA98308.1
AP001918 Genomic DNA Translation: BAA97944.1
K03086 Genomic DNA Translation: AAA92543.1
K03087 Genomic DNA Translation: AAA92545.1

Protein sequence database of the Protein Information Resource

More...
PIRi
A23106 YQECFX
A29332 YQECF

NCBI Reference Sequences

More...
RefSeqi
NP_061453.1, NC_002483.1
NP_862919.1, NC_004998.1
WP_000994779.1, NZ_CP014273.1
YP_003937617.1, NC_014615.1
YP_009060156.1, NC_024956.1
YP_009068351.1, NC_025139.1
YP_009070616.1, NC_025175.1
YP_009071230.1, NC_025179.1

Genome annotation databases

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
1263548
1446483
20466906
20491481
20492684
20493302
9846166

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|83333.107.peg.639

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K01147 Genomic DNA Translation: AAA24910.1
U01159 Genomic DNA Translation: AAC44177.1
M11322 Genomic DNA Translation: AAA98308.1
AP001918 Genomic DNA Translation: BAA97944.1
K03086 Genomic DNA Translation: AAA92543.1
K03087 Genomic DNA Translation: AAA92545.1
PIRiA23106 YQECFX
A29332 YQECF
RefSeqiNP_061453.1, NC_002483.1
NP_862919.1, NC_004998.1
WP_000994779.1, NZ_CP014273.1
YP_003937617.1, NC_014615.1
YP_009060156.1, NC_024956.1
YP_009068351.1, NC_025139.1
YP_009070616.1, NC_025175.1
YP_009071230.1, NC_025179.1

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...
PDBei

Protein Data Bank RCSB

More...
RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5LERelectron microscopy5.001A/1B/1C/1D/1E/1F/1G/1H/1I/1J/1K/1L/1M/1N/1O/2A/2B/2C/2D/2E/2F/2G/2H/2I/2J/2K/2L/2M/2N/2O57-121[»]
5LFBelectron microscopy5.001A/1B/1C/1D/1E/1F/1G/1H/1I/1J/1K/1L/1M/1N/1O/2A/2B/2C/2D/2E/2F/2G/2H/2I/2J/2K/2L/2M/2N/2O57-121[»]
ProteinModelPortaliP04737
SMRiP04737
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP04737, 1 interactor

PTM databases

iPTMnetiP04737

Proteomic databases

PRIDEiP04737

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1263548
1446483
20466906
20491481
20492684
20493302
9846166
PATRICifig|83333.107.peg.639

Miscellaneous databases

Protein Ontology

More...
PROi
PR:P04737

Family and domain databases

InterProiView protein in InterPro
IPR008873 TraA
PfamiView protein in Pfam
PF05513 TraA, 1 hit
TIGRFAMsiTIGR02758 TraA_TIGR, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiPIL1_ECOLI
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P04737
Secondary accession number(s): P14517
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: November 7, 2018
This is version 102 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
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