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UniProtKB - P04693 (TYRB_ECOLI)
Protein
Aromatic-amino-acid aminotransferase
Gene
tyrB
Organism
Escherichia coli (strain K12)
Status
Functioni
Broad-specificity enzyme that catalyzes the transamination of 2-ketoisocaproate, p-hydroxyphenylpyruvate, and phenylpyruvate to yield leucine, tyrosine, and phenylalanine, respectively. In vitro, is able to catalyze the conversion of beta-methyl phenylpyruvate to the nonproteinogenic amino acid (2S,3S)-beta-methyl-phenylalanine, a building block of the antibiotic mannopeptimycin produced by Streptomyces hygroscopicus NRRL3085.
1 PublicationCatalytic activityi
- EC:2.6.1.571 Publication
- (2S,3S)-3-methylphenylalanine + 2-oxoglutarate = (3S)-2-oxo-3-phenylbutanoate + L-glutamate1 PublicationEC:2.6.1.1071 Publication
Cofactori
: L-phenylalanine biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes L-phenylalanine from phenylpyruvate (ArAT route). This subpathway is part of the pathway L-phenylalanine biosynthesis, which is itself part of Amino-acid biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-phenylalanine from phenylpyruvate (ArAT route), the pathway L-phenylalanine biosynthesis and in Amino-acid biosynthesis.
Pathwayi: L-tyrosine biosynthesis
This protein is involved in step 1 of the subpathway that synthesizes L-tyrosine from (4-hydroxyphenyl)pyruvate. This subpathway is part of the pathway L-tyrosine biosynthesis, which is itself part of Amino-acid biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tyrosine from (4-hydroxyphenyl)pyruvate, the pathway L-tyrosine biosynthesis and in Amino-acid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 34 | Substrate; via amide nitrogenBy similarity | 1 | |
Binding sitei | 66 | SubstrateBy similarity | 1 | |
Binding sitei | 131 | SubstrateBy similarity | 1 | |
Binding sitei | 184 | SubstrateBy similarity | 1 | |
Binding sitei | 281 | SubstrateBy similarity | 1 | |
Binding sitei | 375 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- aromatic-amino-acid:2-oxoglutarate aminotransferase activity Source: EcoCyc
- identical protein binding Source: GO_Central
- L-leucine:2-oxoglutarate aminotransferase activity Source: EcoCyc
- L-phenylalanine:2-oxoglutarate aminotransferase activity Source: UniProtKB-EC
- L-tyrosine:2-oxoglutarate aminotransferase activity Source: EcoCyc
- pyridoxal phosphate binding Source: EcoliWiki
- transaminase activity Source: GO_Central
GO - Biological processi
- aspartate biosynthetic process Source: EcoliWiki
- leucine biosynthetic process Source: EcoCyc
- L-phenylalanine biosynthetic process from chorismate via phenylpyruvate Source: EcoCyc
- tyrosine biosynthetic process from chorismate via 4-hydroxyphenylpyruvate Source: EcoCyc
Keywordsi
Molecular function | Aminotransferase, Transferase |
Biological process | Amino-acid biosynthesis, Aromatic amino acid biosynthesis |
Ligand | Pyridoxal phosphate |
Enzyme and pathway databases
BioCyci | EcoCyc:TYRB-MONOMER |
UniPathwayi | UPA00121;UER00347 UPA00122;UER00350 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:tyrB Ordered Locus Names:b4054, JW4014 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000123892 | 1 – 397 | Aromatic-amino-acid aminotransferaseAdd BLAST | 397 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 247 | N6-(pyridoxal phosphate)lysine | 1 |
Proteomic databases
jPOSTi | P04693 |
PaxDbi | P04693 |
PRIDEi | P04693 |
Interactioni
Subunit structurei
Homodimer.
GO - Molecular functioni
- identical protein binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 4262667, 9 interactors |
IntActi | P04693, 2 interactors |
STRINGi | 511145.b4054 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P04693 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P04693 |
Family & Domainsi
Sequence similaritiesi
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.Curated
Phylogenomic databases
eggNOGi | COG1448, Bacteria |
HOGENOMi | CLU_032440_1_2_6 |
InParanoidi | P04693 |
PhylomeDBi | P04693 |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
InterProi | View protein in InterPro IPR004839, Aminotransferase_I/II IPR000796, Asp_trans IPR004838, NHTrfase_class1_PyrdxlP-BS IPR015424, PyrdxlP-dep_Trfase IPR015421, PyrdxlP-dep_Trfase_major IPR015422, PyrdxlP-dep_Trfase_small |
PANTHERi | PTHR11879, PTHR11879, 1 hit |
Pfami | View protein in Pfam PF00155, Aminotran_1_2, 1 hit |
PRINTSi | PR00799, TRANSAMINASE |
SUPFAMi | SSF53383, SSF53383, 1 hit |
PROSITEi | View protein in PROSITE PS00105, AA_TRANSFER_CLASS_1, 1 hit |
i Sequence
Sequence statusi: Complete.
P04693-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFQKVDAYAG DPILTLMERF KEDPRSDKVN LSIGLYYNED GIIPQLQAVA
60 70 80 90 100
EAEARLNAQP HGASLYLPME GLNCYRHAIA PLLFGADHPV LKQQRVATIQ
110 120 130 140 150
TLGGSGALKV GADFLKRYFP ESGVWVSDPT WENHVAIFAG AGFEVSTYPW
160 170 180 190 200
YDEATNGVRF NDLLATLKTL PARSIVLLHP CCHNPTGADL TNDQWDAVIE
210 220 230 240 250
ILKARELIPF LDIAYQGFGA GMEEDAYAIR AIASAGLPAL VSNSFSKIFS
260 270 280 290 300
LYGERVGGLS VMCEDAEAAG RVLGQLKATV RRNYSSPPNF GAQVVAAVLN
310 320 330 340 350
DEALKASWLA EVEEMRTRIL AMRQELVKVL STEMPERNFD YLLNQRGMFS
360 370 380 390
YTGLSAAQVD RLREEFGVYL IASGRMCVAG LNTANVQRVA KAFAAVM
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X03628 Genomic DNA Translation: CAA27278.1 M12047 Genomic DNA Translation: AAA24703.1 U00006 Genomic DNA Translation: AAC43148.1 U00096 Genomic DNA Translation: AAC77024.1 AP009048 Genomic DNA Translation: BAE78056.1 M17809 Genomic DNA Translation: AAA24704.1 |
PIRi | A30379, XNECY |
RefSeqi | NP_418478.1, NC_000913.3 WP_000486985.1, NZ_SSZK01000016.1 |
Genome annotation databases
EnsemblBacteriai | AAC77024; AAC77024; b4054 BAE78056; BAE78056; BAE78056 |
GeneIDi | 948563 |
KEGGi | ecj:JW4014 eco:b4054 |
PATRICi | fig|511145.12.peg.4173 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X03628 Genomic DNA Translation: CAA27278.1 M12047 Genomic DNA Translation: AAA24703.1 U00006 Genomic DNA Translation: AAC43148.1 U00096 Genomic DNA Translation: AAC77024.1 AP009048 Genomic DNA Translation: BAE78056.1 M17809 Genomic DNA Translation: AAA24704.1 |
PIRi | A30379, XNECY |
RefSeqi | NP_418478.1, NC_000913.3 WP_000486985.1, NZ_SSZK01000016.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3FSL | X-ray | 2.35 | A/B/C/D/E/F | 1-397 | [»] | |
3TAT | X-ray | 3.50 | A/B/C/D/E/F | 1-397 | [»] | |
SMRi | P04693 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262667, 9 interactors |
IntActi | P04693, 2 interactors |
STRINGi | 511145.b4054 |
Proteomic databases
jPOSTi | P04693 |
PaxDbi | P04693 |
PRIDEi | P04693 |
Genome annotation databases
EnsemblBacteriai | AAC77024; AAC77024; b4054 BAE78056; BAE78056; BAE78056 |
GeneIDi | 948563 |
KEGGi | ecj:JW4014 eco:b4054 |
PATRICi | fig|511145.12.peg.4173 |
Organism-specific databases
EchoBASEi | EB1033 |
Phylogenomic databases
eggNOGi | COG1448, Bacteria |
HOGENOMi | CLU_032440_1_2_6 |
InParanoidi | P04693 |
PhylomeDBi | P04693 |
Enzyme and pathway databases
UniPathwayi | UPA00121;UER00347 UPA00122;UER00350 |
BioCyci | EcoCyc:TYRB-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P04693 |
PROi | PR:P04693 |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
InterProi | View protein in InterPro IPR004839, Aminotransferase_I/II IPR000796, Asp_trans IPR004838, NHTrfase_class1_PyrdxlP-BS IPR015424, PyrdxlP-dep_Trfase IPR015421, PyrdxlP-dep_Trfase_major IPR015422, PyrdxlP-dep_Trfase_small |
PANTHERi | PTHR11879, PTHR11879, 1 hit |
Pfami | View protein in Pfam PF00155, Aminotran_1_2, 1 hit |
PRINTSi | PR00799, TRANSAMINASE |
SUPFAMi | SSF53383, SSF53383, 1 hit |
PROSITEi | View protein in PROSITE PS00105, AA_TRANSFER_CLASS_1, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TYRB_ECOLI | |
Accessioni | P04693Primary (citable) accession number: P04693 Secondary accession number(s): Q2M6Q0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 13, 1987 |
Last sequence update: | August 13, 1987 | |
Last modified: | February 23, 2022 | |
This is version 180 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families