UniProtKB - P04666 (NCAP_INBSI)
Protein
Nucleoprotein
Gene
NP
Organism
Influenza B virus (strain B/Singapore/222/1979)
Status
Functioni
Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the host nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals that are responsible for the active RNP import into the nucleus through cellular importin alpha/beta pathway. Later in the infection, nclear export of RNPs are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that nucleoprotein binds directly host exportin-1/XPO1 and plays an active role in RNPs nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmasks nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus.UniRule annotation
GO - Molecular functioni
- RNA binding Source: UniProtKB-UniRule
- structural molecule activity Source: UniProtKB-UniRule
GO - Biological processi
- viral entry into host cell Source: UniProtKB-KW
- viral penetration into host nucleus Source: UniProtKB-UniRule
Keywordsi
Molecular function | Ribonucleoprotein, RNA-binding, Viral nucleoprotein |
Biological process | Host-virus interaction, Viral penetration into host nucleus, Virus entry into host cell |
Names & Taxonomyi
Protein namesi | Recommended name: NucleoproteinUniRule annotationAlternative name(s): Nucleocapsid proteinUniRule annotation Short name: Protein NUniRule annotation |
Gene namesi | Name:NPUniRule annotation |
Organismi | Influenza B virus (strain B/Singapore/222/1979) |
Taxonomic identifieri | 107417 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Orthornavirae › Negarnaviricota › Polyploviricotina › Insthoviricetes › Articulavirales › Orthomyxoviridae › Betainfluenzavirus › |
Virus hosti | Homo sapiens (Human) [TaxID: 9606] |
Subcellular locationi
- Virion UniRule annotation
- Host nucleus UniRule annotation
GO - Cellular componenti
- helical viral capsid Source: UniProtKB-UniRule
- host cell nucleus Source: UniProtKB-SubCell
- viral nucleocapsid Source: UniProtKB-UniRule
Keywords - Cellular componenti
Capsid protein, Helical capsid protein, Host nucleus, VirionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000079144 | 1 – 560 | NucleoproteinAdd BLAST | 560 |
Post-translational modificationi
Late in virus-infected cells, may be cleaved from a 56-kDa protein to a 53-kDa protein by a cellular caspase. This cleavage might be a marker for the onset of apoptosis in infected cells or have a specific function in virus host interaction.UniRule annotation
Interactioni
Subunit structurei
Homomultimerizes to form the nucleocapsid. May bind host exportin-1/XPO1. Binds to viral genomic RNA. Protein-RNA contacts are mediated by a combination of electrostatic interactions between positively charged residues and the phosphate backbone and planar interactions between aromatic side chains and bases.
UniRule annotationFamily & Domainsi
Sequence similaritiesi
Belongs to the influenza viruses nucleoprotein family.UniRule annotation
Family and domain databases
HAMAPi | MF_04070, INFV_NCAP, 1 hit |
InterProi | View protein in InterPro IPR002141, Flu_NP |
Pfami | View protein in Pfam PF00506, Flu_NP, 1 hit |
i Sequence
Sequence statusi: Complete.
P04666-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSNMDIDGIN TGTIDKTPEE IISGTSGATR PIIRPATLAP PSNKRTRNPS
60 70 80 90 100
PERATTSSEA DVGRKTQKKQ TPTEIKKSVY NMVVKLGEFY NQMMVKAGLN
110 120 130 140 150
DDMERNLIQN AHAVERILLA ATDDKKTEFQ KKKNARDVKE GKEEIDHNKT
160 170 180 190 200
GGTFYKMVRD DKTIYFSPIR ITFLKEEVKT MYKTTMGSDG FSGLNHIMIG
210 220 230 240 250
HSQMNDVCFQ RSKALKRVGL DPSLISTFAG STLPRRSGAT GVAIKGGGTL
260 270 280 290 300
VAEAIRFIGR AMADRGLLRD IKAKTAYEKI LLNLKNKCSA PQQKALVDQV
310 320 330 340 350
IGSRNPGIAD IEDLTLLARS MVVVRPSVAS KVVLPISIYA KIPQLGFNVE
360 370 380 390 400
EYSMVGYEAM ALYNMATPVS ILRMGDDAKD KSQLFFMSCF GAAYEDLRVL
410 420 430 440 450
SALTGTEFKP RSALKCKGFH VPAKEQVEGM GAALMSIKLQ FWAPMTRSGG
460 470 480 490 500
NEVGGDGGSG QISCSPVFAV ERPIALSKQA VRRMLSMNIE GRDADVKGNL
510 520 530 540 550
LKMMNDSMAK KTNGNAFIGK KMFQISDKNK TNPVEIPIKQ TIPNFFFGRD
560
TAEDYDDLDY
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | K01139 Genomic RNA Translation: AAA43750.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | K01139 Genomic RNA Translation: AAA43750.1 |
3D structure databases
SMRi | P04666 |
ModBasei | Search... |
Family and domain databases
HAMAPi | MF_04070, INFV_NCAP, 1 hit |
InterProi | View protein in InterPro IPR002141, Flu_NP |
Pfami | View protein in Pfam PF00506, Flu_NP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NCAP_INBSI | |
Accessioni | P04666Primary (citable) accession number: P04666 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 13, 1987 |
Last sequence update: | August 13, 1987 | |
Last modified: | December 2, 2020 | |
This is version 80 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |