UniProtKB - P04633 (UCP1_RAT)
Protein
Mitochondrial brown fat uncoupling protein 1
Gene
Ucp1
Organism
Rattus norvegicus (Rat)
Status
Functioni
Mitochondrial protein responsible for thermogenic respiration, a specialized capacity of brown adipose tissue and beige fat that participates in non-shivering adaptive thermogenesis to temperature and diet variations and more generally to the regulation of energy balance (By similarity). Functions as a long-chain fatty acid/LCFA and proton symporter, simultaneously transporting one LCFA and one proton through the inner mitochondrial membrane. However, LCFAs remaining associated with the transporter via their hydrophobic tails, it results in an apparent transport of protons activated by LCFAs (PubMed:12479871, PubMed:16814247). Thereby, dissipates the mitochondrial proton gradient and converts the energy of substrate oxydation into heat instead of ATP. Regulates the production of reactive oxygen species/ROS by mitochondria (By similarity).By similarity2 Publications
Activity regulationi
Has no constitutive proton transporter activity and has to be activated by long-chain fatty acids/LCFAs. Inhibited by purine nucleotides. Both purine nucleotides and LCFAs bind the cytosolic side of the transporter and directly compete to activate or inhibit it (PubMed:22952235). Activated by noradrenaline and reactive oxygen species (By similarity).By similarity1 Publication
GO - Molecular functioni
- cardiolipin binding Source: UniProtKB
- GDP binding Source: UniProtKB
- GTP binding Source: UniProtKB
- long-chain fatty acid binding Source: UniProtKB
- oxidative phosphorylation uncoupler activity Source: UniProtKB
- purine ribonucleotide binding Source: RGD
- transmembrane transporter activity Source: UniProtKB
GO - Biological processi
- adaptive thermogenesis Source: UniProtKB
- brown fat cell differentiation Source: RGD
- cellular response to dehydroepiandrosterone Source: RGD
- cellular response to fatty acid Source: UniProtKB
- cellular response to hormone stimulus Source: RGD
- cellular response to reactive oxygen species Source: UniProtKB
- diet induced thermogenesis Source: RGD
- generation of precursor metabolites and energy Source: RGD
- mitochondrial transmembrane transport Source: UniProtKB
- mitochondrial transport Source: RGD
- positive regulation of cold-induced thermogenesis Source: YuBioLab
- positive regulation of metabolic process Source: RGD
- proton transmembrane transport Source: UniProtKB
- regulation of reactive oxygen species biosynthetic process Source: RGD
- regulation of transcription by RNA polymerase II Source: RGD
- response to cold Source: GO_Central
- response to nutrient levels Source: UniProtKB
- response to temperature stimulus Source: UniProtKB
Keywordsi
Molecular function | Ion channel |
Biological process | Ion transport, Transport |
Enzyme and pathway databases
Reactomei | R-RNO-167826, The fatty acid cycling model R-RNO-167827, The proton buffering model |
Names & Taxonomyi
Protein namesi | Recommended name: Mitochondrial brown fat uncoupling protein 1CuratedShort name: UCP 1Curated Alternative name(s): Solute carrier family 25 member 7By similarity ThermogeninBy similarity |
Gene namesi | |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 3931, Ucp1 |
Subcellular locationi
Mitochondrion
- Mitochondrion inner membrane By similarity; Multi-pass membrane protein 1 Publication
Mitochondrion
- mitochondrial envelope Source: RGD
- mitochondrial inner membrane Source: UniProtKB
- mitochondrion Source: UniProtKB
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 10 | Mitochondrial intermembrane1 Publication | 10 | |
Transmembranei | 11 – 32 | Helical; Name=1Sequence analysisAdd BLAST | 22 | |
Topological domaini | 33 – 73 | Mitochondrial matrix1 PublicationAdd BLAST | 41 | |
Transmembranei | 74 – 96 | Helical; Name=2Sequence analysisAdd BLAST | 23 | |
Topological domaini | 97 – 116 | Mitochondrial intermembrane1 PublicationAdd BLAST | 20 | |
Transmembranei | 117 – 133 | Helical; Name=3Sequence analysisAdd BLAST | 17 | |
Topological domaini | 134 – 178 | Mitochondrial matrix1 PublicationAdd BLAST | 45 | |
Transmembranei | 179 – 195 | Helical; Name=4Sequence analysisAdd BLAST | 17 | |
Topological domaini | 196 – 212 | Mitochondrial intermembrane1 PublicationAdd BLAST | 17 | |
Transmembranei | 213 – 232 | Helical; Name=5Sequence analysisAdd BLAST | 20 | |
Topological domaini | 233 – 266 | Mitochondrial matrix1 PublicationAdd BLAST | 34 | |
Transmembranei | 267 – 289 | Helical; Name=6Sequence analysisAdd BLAST | 23 | |
Topological domaini | 290 – 307 | Mitochondrial intermembrane1 PublicationAdd BLAST | 18 |
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion inner membranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 25 | C → A: No effect on GTP-binding. Loss of fatty acid-induced proton transport; when associated with V-28 and A-31. 1 Publication | 1 | |
Mutagenesisi | 28 | D → V: No effect on GTP-binding. Decreased fatty acid-induced proton transport. Loss of fatty acid-induced proton transport; when associated with A-25 and A-31. 1 Publication | 1 | |
Mutagenesisi | 31 | T → A: No effect on GTP-binding. No effect on fatty acid-induced proton transport. Loss of fatty acid-induced proton transport; when associated with A-25 and V-28. 1 Publication | 1 | |
Mutagenesisi | 146 | H → L: No effect on GTP-binding. Decreased fatty acid-induced proton transport; when associated with L-148. 1 Publication | 1 | |
Mutagenesisi | 148 | H → L: No effect on GTP-binding. Decreased fatty acid-induced proton transport; when associated with L-146. 1 Publication | 1 | |
Mutagenesisi | 153 | R → L: No effect on GTP-binding. Decreased fatty acid-induced proton transport. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000090662 | 1 – 307 | Mitochondrial brown fat uncoupling protein 1Add BLAST | 307 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 254 | Cysteine sulfenic acid (-SOH)By similarity | 1 |
Post-translational modificationi
May undergo ubiquitin-mediated proteasomal degradation.1 Publication
May undergo sulfenylation upon cold exposure. May increase the sensitivity of UCP1 thermogenic function to the activation by noradrenaline probably through structural effects.By similarity
Keywords - PTMi
OxidationProteomic databases
PaxDbi | P04633 |
PRIDEi | P04633 |
PTM databases
iPTMneti | P04633 |
PhosphoSitePlusi | P04633 |
Expressioni
Tissue specificityi
Brown adipose tissue.1 Publication
Gene expression databases
Bgeei | ENSRNOG00000003580, Expressed in thymus and 8 other tissues |
Genevisiblei | P04633, RN |
Interactioni
Subunit structurei
Most probably functions as a monomer (By similarity). Binds one purine nucleotide per monomer (PubMed:12479871, PubMed:22952235). However, has also been suggested to function as a homodimer or a homotetramer (By similarity). Tightly associates with cardiolipin in the mitochondrion inner membrane; may stabilize and regulate its activity (By similarity).
By similarity2 PublicationsProtein-protein interaction databases
BioGRIDi | 246975, 1 interactor |
STRINGi | 10116.ENSRNOP00000004900 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 11 – 102 | Solcar 1Add BLAST | 92 | |
Repeati | 111 – 201 | Solcar 2Add BLAST | 91 | |
Repeati | 210 – 295 | Solcar 3Add BLAST | 86 |
Sequence similaritiesi
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0753, Eukaryota |
GeneTreei | ENSGT00940000160382 |
HOGENOMi | CLU_015166_14_2_1 |
InParanoidi | P04633 |
OMAi | NCAMKMF |
OrthoDBi | 984118at2759 |
PhylomeDBi | P04633 |
TreeFami | TF323211 |
Family and domain databases
Gene3Di | 1.50.40.10, 1 hit |
InterProi | View protein in InterPro IPR002030, Mit_uncoupling_UCP-like IPR018108, Mitochondrial_sb/sol_carrier IPR023395, Mt_carrier_dom_sf IPR040011, UCP1 |
PANTHERi | PTHR45618:SF19, PTHR45618:SF19, 1 hit |
Pfami | View protein in Pfam PF00153, Mito_carr, 3 hits |
PRINTSi | PR00784, MTUNCOUPLING |
SUPFAMi | SSF103506, SSF103506, 1 hit |
PROSITEi | View protein in PROSITE PS50920, SOLCAR, 3 hits |
i Sequence
Sequence statusi: Complete.
P04633-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVSSTTSEVQ PTMGVKIFSA GVSACLADII TFPLDTAKVR LQIQGEGQAS
60 70 80 90 100
STIRYKGVLG TITTLAKTEG LPKLYSGLPA GIQRQISFAS LRIGLYDTVQ
110 120 130 140 150
EYFSSGRETP ASLGSKISAG LMTGGVAVFI GQPTEVVKVR MQAQSHLHGI
160 170 180 190 200
KPRYTGTYNA YRVIATTESL STLWKGTTPN LMRNVIINCT ELVTYDLMKG
210 220 230 240 250
ALVNHHILAD DVPCHLLSAL VAGFCTTLLA SPVDVVKTRF INSLPGQYPS
260 270 280 290 300
VPSCAMTMYT KEGPAAFFKG FAPSFLRLGS WNVIMFVCFE QLKKELMKSR
QTVDCTT
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M11814 mRNA Translation: AAA19671.1 X03894 mRNA Translation: CAA27531.1 BC088156 mRNA Translation: AAH88156.1 X12925 Genomic DNA Translation: CAA31392.1 |
PIRi | A26294 |
RefSeqi | NP_036814.1, NM_012682.2 |
Genome annotation databases
Ensembli | ENSRNOT00000004900; ENSRNOP00000004900; ENSRNOG00000003580 |
GeneIDi | 24860 |
KEGGi | rno:24860 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M11814 mRNA Translation: AAA19671.1 X03894 mRNA Translation: CAA27531.1 BC088156 mRNA Translation: AAH88156.1 X12925 Genomic DNA Translation: CAA31392.1 |
PIRi | A26294 |
RefSeqi | NP_036814.1, NM_012682.2 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 246975, 1 interactor |
STRINGi | 10116.ENSRNOP00000004900 |
PTM databases
iPTMneti | P04633 |
PhosphoSitePlusi | P04633 |
Proteomic databases
PaxDbi | P04633 |
PRIDEi | P04633 |
Genome annotation databases
Ensembli | ENSRNOT00000004900; ENSRNOP00000004900; ENSRNOG00000003580 |
GeneIDi | 24860 |
KEGGi | rno:24860 |
Organism-specific databases
CTDi | 7350 |
RGDi | 3931, Ucp1 |
Phylogenomic databases
eggNOGi | KOG0753, Eukaryota |
GeneTreei | ENSGT00940000160382 |
HOGENOMi | CLU_015166_14_2_1 |
InParanoidi | P04633 |
OMAi | NCAMKMF |
OrthoDBi | 984118at2759 |
PhylomeDBi | P04633 |
TreeFami | TF323211 |
Enzyme and pathway databases
Reactomei | R-RNO-167826, The fatty acid cycling model R-RNO-167827, The proton buffering model |
Miscellaneous databases
PROi | PR:P04633 |
Gene expression databases
Bgeei | ENSRNOG00000003580, Expressed in thymus and 8 other tissues |
Genevisiblei | P04633, RN |
Family and domain databases
Gene3Di | 1.50.40.10, 1 hit |
InterProi | View protein in InterPro IPR002030, Mit_uncoupling_UCP-like IPR018108, Mitochondrial_sb/sol_carrier IPR023395, Mt_carrier_dom_sf IPR040011, UCP1 |
PANTHERi | PTHR45618:SF19, PTHR45618:SF19, 1 hit |
Pfami | View protein in Pfam PF00153, Mito_carr, 3 hits |
PRINTSi | PR00784, MTUNCOUPLING |
SUPFAMi | SSF103506, SSF103506, 1 hit |
PROSITEi | View protein in PROSITE PS50920, SOLCAR, 3 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | UCP1_RAT | |
Accessioni | P04633Primary (citable) accession number: P04633 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 13, 1987 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 153 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families