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Protein

Serine/threonine-protein kinase A-Raf

Gene

Araf

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi99Zinc 1By similarity1
Metal bindingi112Zinc 2By similarity1
Metal bindingi115Zinc 2By similarity1
Metal bindingi125Zinc 1By similarity1
Metal bindingi128Zinc 1By similarity1
Metal bindingi133Zinc 2By similarity1
Metal bindingi136Zinc 2By similarity1
Metal bindingi144Zinc 1By similarity1
Binding sitei334ATPPROSITE-ProRule annotation1
Active sitei427Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri98 – 144Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST47
Nucleotide bindingi314 – 322ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDAi2.7.10.2 3474
ReactomeiR-MMU-5673000 RAF activation
R-MMU-5674135 MAP2K and MAPK activation
R-MMU-5675221 Negative regulation of MAPK pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase A-Raf (EC:2.7.11.1)
Alternative name(s):
Proto-oncogene A-Raf
Gene namesi
Name:Araf
Synonyms:A-raf, Araf1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:88065 Araf

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000856231 – 604Serine/threonine-protein kinase A-RafAdd BLAST604

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei157PhosphoserineCombined sources1
Modified residuei162PhosphoserineBy similarity1
Modified residuei181PhosphothreonineCombined sources1
Modified residuei186PhosphoserineBy similarity1
Modified residuei255PhosphoserineBy similarity1
Modified residuei267PhosphoserineBy similarity1
Modified residuei316PhosphothreonineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP04627
MaxQBiP04627
PaxDbiP04627
PeptideAtlasiP04627
PRIDEiP04627

PTM databases

iPTMnetiP04627
PhosphoSitePlusiP04627

Expressioni

Gene expression databases

BgeeiENSMUSG00000001127 Expressed in 284 organ(s), highest expression level in dorsomedial nucleus of hypothalamus
CleanExiMM_ARAF
ExpressionAtlasiP04627 baseline and differential
GenevisibleiP04627 MM

Interactioni

Subunit structurei

Interacts with TH1L/NELFD.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198180, 1 interactor
DIPiDIP-1070N
IntActiP04627, 6 interactors
MINTiP04627
STRINGi10090.ENSMUSP00000001155

Structurei

3D structure databases

ProteinModelPortaliP04627
SMRiP04627
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini19 – 91RBDPROSITE-ProRule annotationAdd BLAST73
Domaini308 – 568Protein kinasePROSITE-ProRule annotationAdd BLAST261

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri98 – 144Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST47

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG0193 Eukaryota
ENOG410Y4UP LUCA
GeneTreeiENSGT00900000140880
HOGENOMiHOG000252972
HOVERGENiHBG001886
InParanoidiP04627
KOiK08845
OMAiQHRDPEH
OrthoDBiEOG091G09SB
PhylomeDBiP04627
TreeFamiTF317006

Family and domain databases

CDDicd00029 C1, 1 hit
InterProiView protein in InterPro
IPR020454 DAG/PE-bd
IPR011009 Kinase-like_dom_sf
IPR002219 PE/DAG-bd
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR003116 RBD_dom
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
IPR008271 Ser/Thr_kinase_AS
IPR029071 Ubiquitin-like_domsf
PfamiView protein in Pfam
PF00130 C1_1, 1 hit
PF07714 Pkinase_Tyr, 1 hit
PF02196 RBD, 1 hit
PRINTSiPR00008 DAGPEDOMAIN
SMARTiView protein in SMART
SM00109 C1, 1 hit
SM00455 RBD, 1 hit
SM00220 S_TKc, 1 hit
SUPFAMiSSF54236 SSF54236, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
PS50898 RBD, 1 hit
PS00479 ZF_DAG_PE_1, 1 hit
PS50081 ZF_DAG_PE_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All

P04627-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEPPRGPPVS GAEPSRAVGT VKVYLPNKQR TVVTVREGMS VYDSLDKALK
60 70 80 90 100
VRGLNQDCCV VYRLIKGRKT VTAWDTAIAP LDGEELIVEV LEDVPLTMHN
110 120 130 140 150
FVRKTFFSLA FCDFCLKFLF HGFRCQTCGY KFHQHCSSKV PTVCVDMSTN
160 170 180 190 200
RRQFYHSIQD LSGGSRQQEA PSNLSVNELL TPQGPSPFTQ QRDQEHFSFP
210 220 230 240 250
APANPPLQRI RSTSTPNVHM VSTTAPMDSS LMQFTAQSFS TDAAGRGGDG
260 270 280 290 300
APRGSPSPAS VSSGRKSPHS KLPSEQRERK SLADEKKKVK NLGYRDSGYY
310 320 330 340 350
WEVPPSEVQL LKRIGTGSFG TVFRGRWHGD VAVKVLKVAQ PTAEQAQAFK
360 370 380 390 400
NEMQVLRKTR HVNILLFMGF MTRPGFAIIT QWCEGSSLYH HLHVADTRFD
410 420 430 440 450
MVQLIDVARQ TAQGMDYLHA KNIIHRDLKS NNIFLHEGLT VKIGDFGLAT
460 470 480 490 500
VKTRWSGAQP LEQPSGSVLW MAAEVIRMQD PNPYSFQSDV YAYGVVLYEL
510 520 530 540 550
MTGSLPYSHI GSRDQIIFMV GRGYLSPDLS KIFSNCPKAM RRLLTDCLKF
560 570 580 590 600
QREERPLFPQ ILATIELLQR SLPKIERSAS EPSLHRTQAD ELPACLLSAA

RLVP
Length:604
Mass (Da):67,581
Last modified:August 2, 2002 - v2
Checksum:i05F8262F99DDD087
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9Q0P8E9Q0P8_MOUSE
Serine/threonine-protein kinase A-R...
Araf
419Annotation score:
Q8CAD1Q8CAD1_MOUSE
Ras-binding protein DA-Raf
Araf
186Annotation score:
B1AUN8B1AUN8_MOUSE
Serine/threonine-protein kinase A-R...
Araf
230Annotation score:
B1AUP0B1AUP0_MOUSE
Serine/threonine-protein kinase A-R...
Araf
203Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti169E → K in BAA00018 (PubMed:3491291).Curated1
Sequence conflicti186S → R in BAB23522 (PubMed:16141072).Curated1
Sequence conflicti186S → R in BAB26674 (PubMed:16141072).Curated1
Sequence conflicti326R → L in BAA00018 (PubMed:3491291).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL671885 Genomic DNA No translation available.
BC004757 mRNA Translation: AAH04757.1
AK004741 mRNA Translation: BAB23522.1
AK010060 mRNA Translation: BAB26674.1
AK020547 mRNA Translation: BAB32131.3
D00024 mRNA Translation: BAA00018.1
CCDSiCCDS40886.1
PIRiA25382 TVMSRF
RefSeqiNP_001153117.1, NM_001159645.1
NP_033833.1, NM_009703.2
XP_006527619.1, XM_006527556.3
XP_006527620.1, XM_006527557.3
UniGeneiMm.220946

Genome annotation databases

EnsembliENSMUST00000001155; ENSMUSP00000001155; ENSMUSG00000001127
GeneIDi11836
KEGGimmu:11836
UCSCiuc009stt.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL671885 Genomic DNA No translation available.
BC004757 mRNA Translation: AAH04757.1
AK004741 mRNA Translation: BAB23522.1
AK010060 mRNA Translation: BAB26674.1
AK020547 mRNA Translation: BAB32131.3
D00024 mRNA Translation: BAA00018.1
CCDSiCCDS40886.1
PIRiA25382 TVMSRF
RefSeqiNP_001153117.1, NM_001159645.1
NP_033833.1, NM_009703.2
XP_006527619.1, XM_006527556.3
XP_006527620.1, XM_006527557.3
UniGeneiMm.220946

3D structure databases

ProteinModelPortaliP04627
SMRiP04627
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198180, 1 interactor
DIPiDIP-1070N
IntActiP04627, 6 interactors
MINTiP04627
STRINGi10090.ENSMUSP00000001155

PTM databases

iPTMnetiP04627
PhosphoSitePlusiP04627

Proteomic databases

EPDiP04627
MaxQBiP04627
PaxDbiP04627
PeptideAtlasiP04627
PRIDEiP04627

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000001155; ENSMUSP00000001155; ENSMUSG00000001127
GeneIDi11836
KEGGimmu:11836
UCSCiuc009stt.2 mouse

Organism-specific databases

CTDi369
MGIiMGI:88065 Araf

Phylogenomic databases

eggNOGiKOG0193 Eukaryota
ENOG410Y4UP LUCA
GeneTreeiENSGT00900000140880
HOGENOMiHOG000252972
HOVERGENiHBG001886
InParanoidiP04627
KOiK08845
OMAiQHRDPEH
OrthoDBiEOG091G09SB
PhylomeDBiP04627
TreeFamiTF317006

Enzyme and pathway databases

BRENDAi2.7.10.2 3474
ReactomeiR-MMU-5673000 RAF activation
R-MMU-5674135 MAP2K and MAPK activation
R-MMU-5675221 Negative regulation of MAPK pathway

Miscellaneous databases

ChiTaRSiAraf mouse
PROiPR:P04627
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000001127 Expressed in 284 organ(s), highest expression level in dorsomedial nucleus of hypothalamus
CleanExiMM_ARAF
ExpressionAtlasiP04627 baseline and differential
GenevisibleiP04627 MM

Family and domain databases

CDDicd00029 C1, 1 hit
InterProiView protein in InterPro
IPR020454 DAG/PE-bd
IPR011009 Kinase-like_dom_sf
IPR002219 PE/DAG-bd
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR003116 RBD_dom
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
IPR008271 Ser/Thr_kinase_AS
IPR029071 Ubiquitin-like_domsf
PfamiView protein in Pfam
PF00130 C1_1, 1 hit
PF07714 Pkinase_Tyr, 1 hit
PF02196 RBD, 1 hit
PRINTSiPR00008 DAGPEDOMAIN
SMARTiView protein in SMART
SM00109 C1, 1 hit
SM00455 RBD, 1 hit
SM00220 S_TKc, 1 hit
SUPFAMiSSF54236 SSF54236, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
PS50898 RBD, 1 hit
PS00479 ZF_DAG_PE_1, 1 hit
PS50081 ZF_DAG_PE_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiARAF_MOUSE
AccessioniPrimary (citable) accession number: P04627
Secondary accession number(s): B1AUN9
, Q99J44, Q9CTT5, Q9D6R6, Q9DBU7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 2, 2002
Last modified: September 12, 2018
This is version 201 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  2. SIMILARITY comments
    Index of protein domains and families
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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